Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (18): 3676-3688.doi: 10.3864/j.issn.0578-1752.2015.18.011

• HORTICULTURE • Previous Articles     Next Articles

Study on the Molecular Mechanism Controlling Differences in Fruit Texture Formation of Apple Soft/Crisp Strains

ZHANG Rui, ZHANG Zong-ying, GAO Li-ping, JI Xiao-hao, MAO Zhi-quan, XU Hai-feng, WANG Nan, WU Shu-jing, CHEN Xue-sen   

  1. College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong
  • Received:2015-03-23 Online:2015-09-16 Published:2015-09-16

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Abstract: 【Objective】In order to perfect the breeding theory and technology system of functional apple, the molecular mechanism controlling differences in fruit texture formation of soft/crisp strains from the cross progenies of ‘Fuji’ and M. sieversii f. neidzwetzkyana was studied. 【Method】The ethylene production, fruit firmness, crispness, the relative expression intensity of 4 genes related to ethylene biosynthesis (ACS1 etc.) and 30 genes related to fruit softening (PG) were detected in fruits of different development stages of two cross progenies of ‘Fuji’ and M. sieversii f. neidzwetzkyana (‘Hongmian2’ and ‘Hongcui2’). 【Result】Fruit firmness and crispness both declined during development period in both ‘Hongmian2’ and ‘Hongcui2’, but fruit firmness and crispness of ‘Hongcui2’ was significantly higher than that in ‘Hongmian2’ all the time. The ethylene production of ‘Hongmian2’ increased at DAFB 120 d and presented a peak of ethylene production, while the ethylene production of ‘Hongcui2’ didn’t increase significantly at DAFB 120 d and had no obvious peak. The expression patterns of 4 ethylene biosynthesis genes were different. The expression of ACS1, ACO1 and ACO2 in ‘Hongmian2’ during late development period weighed over 94%, while ACS3a showed constitutive expression, occupying 50% of total expression during early and late development period, respectively. The expression periods of 30 genes related to fruit softening were different. With over 70% of the total expression, 15 genes (PL, AF1, EG2 and XET1 etc.) and 5 genes (PG, AF3, XET2, XET10 and XET11) mainly expressed during early development and late development period, respectively. Except 6 genes (PL, AF1 etc.), the expression of other 24 genes (PG etc.) in ‘Hongcui2’ was significantly lower than that in ‘Hongmian2’. 【Conclusion】The appearance of ethylene production peak during late development period and the up-regulated expression of genes related to ethylene biosynthesis and fruit softening during early and late development period resulted in the softening of ‘Hongmian2’ before harvest and the texture difference between ‘Hongmian2’ and ‘Hongcui2’.

Key words: M. sieversii f. neidzwetzkyana, cross progeny, soft/crisp strain, fruit texture difference, molecular mechanism

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