一种分析土壤中酚酸类物质含量的新方法—以连作苹果园土壤为试材

doi:10.3864/j.issn.0578-1752.2013.21.025

Abstract

Abstract: 【Objective】To establish a new protocol based on the ASE-HPLC method, which could determine the content of phenolic acids in orchard soil rapidly and efficiently.【Method】Phlorizin was set as an sample for analysis, which is one of the characteristic phenolic acids in apple orchard soil. Accelerated solvent extraction (ASE) combined with high performance liquid chromatography (HPLC) was used to assess it. Specifically, the parameters of extraction solvent, extraction temperature, pressure and number of cycles were optimized, aiming at exploring the optimal conditions of ASE extraction for phenolic acids.【Result】The optimal conditions of ASE extraction for phenolic acids were as follows: Ethanol as extraction solvent initially, and methanol was the subsequent extraction solvent，extraction temperature at 120℃, a pressure of 10.3 MPa, 2 cycles, every 5 min static time, 60% purge volume, and purge time for 90 s. 【Conclusion】The process was more simple, and with good reproducibility and linearity, correlation coefficient up to 0.99, recovery efficiency between 83% and 98%, detection limit to the amount of 1.3 × 10-4 -2.5 × 10-2 μg•mL-1. ASE-HPLC is an accurate and rapid new protocol to mensurate phenolic acids, which is an improved and practical method.

Key words: replanted apple orchards , phenolic acids , accelerated solvent extraction , performance liquid chromatography

YIN Cheng-Miao-1, WANG Gong-Shuai-1, LI Yuan-Yuan-2, CHE Jin-Shui-3, SHEN Xiang-1, CHEN Xue-Sen-1, MAO Zhi-Quan-1, WU Shu-Jing-1. A New Method for Analysis of Phenolic Acids in the Soil —Soil from Replanted Apple Orchards was Investigated[J].Scientia Agricultura Sinica, 2013, 46(21): 4612-4619.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2013.21.025

https://www.chinaagrisci.com/EN/Y2013/V46/I21/4612

References

[1]Narwal S. Allelopathy in ecological sustainable organic agriculture. Allelopathy Journal, 2010, 25(1): 51-72.

[2]孔垂华, 胡飞. 植物化感相生相克作用及其应用. 北京: 中国农业出版社, 2001: 30-42.

Kong C H, Hu F. Allelopathic Allelopathy and Its Application. Beijing: China Agriculture Press, 2001: 30-42. (in Chinese)

[3]张江红. 酚类物质对苹果的化感作用及重茬障碍影响机理的研究[D]. 山东泰安:山东农业大学, 2005.

Zhang J H. Allelopathic effect of phenolics and its role on apple replant disease mechanism[D]. Taian, Shandong: Shandong Agricultural University, 2005. (in Chinese)

[4]Kummeler M. Investigations into the causes of soil sickness in fruit trees. Part I. Influence of soil treatment and soil sickness on vegetative growth of Bittenfelder seedling. Erwerbsobstbau, 1981, 23(7): 162-168.

[5]孙海兵, 毛志泉, 朱树华. 环渤海湾地区连作苹果园土壤中酚酸类物质变化. 生态学报, 2011, 31(1): 90-97.

Sun H B, Mao Z Q, Zhu S H. Changes of phenolic acids in the soil of replanted apple orchards surrounding Bohai Gulf. Acta Ecologica Sinica, 2011, 31(1): 90-97. (in Chinese)

[6]张淑香, 高子勤, 刘海玲. 连作障碍与根际微生态研究Ⅲ: 土壤酚酸物质及其生物学效应. 应用生态学报, 2000, 11(5): 741-744.

Zhang S X, Gao Z Q, Liu H L. Continuous cropping obstacle and rhizospheric micro-ecology. Ⅲ. Soil phenolic acids and their biological effect. Chinese Journal of Applied Ecology, 2000, 11(5): 741-744. (in Chinese)

[7]Baerson S R, Dayan F E, Rimando A M, Nanayakkara N P, Liu C J, Schroder J, Fishbein M, Pan Z, Kagan I A, Pratt L H, Cordonnier-Pratt M M, Duke S O. A functional genomics investigation of allelochemical biosynthesis in Sorghum bicolor root hairs. Journal of Biological Chemistry, 2008, 283(6): 3231-3247.

[8]Zhang J H, Mao Z Q, Wang L Q, Shu H R. Bioassay and identification of root exudates of three fruit tree species. Journal of Integrative Plant Biology, 2007, 49(3): 257-261.

[9]Politycka B, Adamska D. Release of phenolic compounds from apple residues decomposing in soil and the influence of temperature on their degradation. Polish Journal of Environmental Studies, 2003, 12(1): 95-98.

[10]Wu H W, Haig T, Pratley J, Lemerle D, An M. Allelochemicals in wheat (Triticum aestivum L.) : variation of phenolic acids in shoot tissues. Journal of Chemical Ecology, 2001, 27(1):125-135.

[11]Ogweno J, Yu J Q. Autotoxic potential in soil sickness: A re-examination. Allelopathy Journal, 2006, 18(1): 93-102.

[12]Jilani G, Mahmood S, Chaudhry A N, Hassan I, Akram M. Allelochemicals: sources, toxicity and microbial transformation in soil: a review. Annals of Microbiology, 2008, 58(3): 351-357.

[13]Hartley R D, Buchan H. High-performance liquid chromatography of phenolic acids and aldehydes derivied from the decomposition of organic matter in soil. Journal of Chromatography, 1979, 180: 139-143.

[14]Haghi G, Hatami A. Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach. Journal of Agricultural and Food Chemistry, 2010, 58: 10812-10816.

[15]Podsedek A, Wilska-Jeszka J, Anders B, Markowski J. Compositional characterisation of some apple varieties. European Food Research and Technology, 2000, 210: 268-272.

[16]Richter B E, Jones B A, Ezzell J L, Porter N L, Avdalovic A, Pohl C. Accelerated solvent extraction: a technique for sample preparation. Analytical Chemistry, 1996, 68(6): 1033-1039.

[17]崔艳红, 巨天珍, 曹军, 陶澍. 加速溶剂提取法测定蔬菜中的多环芳烃和有机氯化合物．农业环境科学学报, 2003, 22(3): 364-367.

Cui Y H, Ju T Z, Cao J, Tao S. Measurement of semi-volatile organic compounds in vegetables using accelerated solvent extraction. Journal of Agro-Environment Science, 2003, 22(3): 364-367. (in Chinese)

[18]赵海香, 袁光耀, 邱月明, 汪丽萍, 周志强. 加速溶剂萃取技术(ASE)在农药残留分析中的应用.农药, 2006, 45(1):15-21.

Zhao H X, Yuan G Y, Qiu Y M, Wang L P, Zhou Z Q. Use of accelerated solvent extraction techniques in pesticide residue analysis. Chinese Journal of Pesticides, 2006, 45(1): 15-21. (in Chinese)

[19]Brachet A, Rudaz S, Mateus L, Christen P, Veuthey J. Optimisation of accelerated solvent extraction of cocaine and benzoylecgonine from coca leaves. Journal of Separation Science, 2001, 24: 865-873.

[20]Eng S O, Shea M L. Pressurized hot water extraction of berbefine, baicalein and glyeyrrhizin in medicinal plants. Analytica Chemica Acta, 2003, 482: 81.

[21]王西奎, 姜建臣. 提取土壤中酚类化合物方法的研究.山东建材学院学报, 1992, 6(4):43-46.

Wang X K, Jiang J C. Study on a extraction method of phenolic compounds in soil. Journal of Shandong Institute of Building Materials, 1992, 6(4): 43-46. (in Chinese)

[22]常春艳, 王云风, 葛宝坤, 刘晹. 利用快速溶剂萃取(ASE)法检测水果和蔬菜中有机氯农药残留. 口岸卫生控制, 2004, 96(6): 25-26.

Chang C Y, Wang Y F, Ge B K, Liu Y. Accelerated solvent extraction (ASE) assay organochlorine pesticide residues in fruits and vegetables. Port health Control, 2004, 96(6): 25-26. (in Chinese)

[23]朱晓兰, 蔡继宝, 杨俊, 苏庆德. 加速溶剂萃取一气相色谱法测定土壤中的有机磷农药残留. 化学分析, 2005, 33(6): 821-824.

Zhu X L, Cai J B, Yang J, Su Q D. Determination of organophosphate pesticide residues in soil by accelerated solvent extraction-gas chromatographic. Chinese Journal of Analytical Chemistry, 2005, 33(6): 821-824. (in Chinese)

[24]王凌, 牟瑛琳, 黎先春. 加速溶剂萃取一气相色谱/质谱(ASE-GC/MS)法测定近海沉积物中的有机磷农药. 中国卫生检验杂志, 2007, 17(5):769-771.

Wang L, Mu Y L, Li X C. Determination of organ phosphorus pesticide in sea-sediment by accelerated solvent extraction-gas chromatography/mass spectrometry. Chinese Journal of Health Laboratory Technology, 2007, 17(5): 769-771. (in Chinese)

[25]龚迎莉, 孙玮琳, 汪双清, 沈斌. 生油岩中有机质加速溶剂萃取和索氏萃取方法对比．岩矿测试, 2009, 28(5): 416-422.

Gong Y L, Sun W L, Wang S Q, Shen B. A Comparative study on extraction of organic matters in source rocks by accelerated solvent extraction and soxhlet extraction. Rock and Mineral Analysis, 2009, 28(5): 416-422. (in Chinese)

[26]庞新安, 刘文杰, 孙红专, 晋玉霞, 万英, 马玲, 孔星芸. 均匀设计法优化巴旦杏仁油的加速溶剂萃取工艺. 中国食物与营养, 2007(4): 43-45.

Pang X A, Liu W J, Sun H Z, Jin Y X, Wan Y, Ma L, Kong X Y. Uniform design almond-oil accelerated solvent extraction process. Food and Nutrition in China, 2007(4): 43-45. (in Chinese)

[27]张玉, 吴慧明, 余建伟, 詹士立, 王建清. 加速溶剂萃取技术提取柑橘皮中总黄酮的工艺研究. 食品科技, 2007, 11: 213-215.

Zhang Y, Wu H M, Yu J W, Zhan S L, Wang J Q. Extraction of total flavonoid from orange peel by accelerated solvent extraction. Food Science and Technology, 2007, 11: 213-215. (in Chinese)

[28]宋文斌, 代英成, 许敏, 李献刚, 于杰. 加速溶剂萃取-液相色谱-紫外检测法测定人参中多种人参皂甙含量. 现代科学仪器, 2009, 6: 104-108.

Song W B, Dai Y C, Xu M, Li X G, Yu J. Determination of ginsenosides in ginseng by ASE-SPE-LC-UV method. Modern Scientific Instruments, 2009, 6: 104-108. (in Chinese)

[29]刘江云, 杨学东, 徐丽珍, 杨世林. 天然酚酸类化合物的反相高效液相色谱分析. 色谱, 2002, 20(3):245-248.

Liu J Y, Yang X D, Xu L Z, Yang S L. Studies on the separation and determination of natural phenolic acids by reversed-phase high performance liquid chromatography. Chinese Journal of Chromatography, 2002, 20(3):245-248. (in Chinese)

[30]Hilt P, Schieber A, Yildirim C. Detection of phloridzin in strawberries (Fragaria x ananassa Duch.) by HPLC-PDA-MS/MS and NMR spectroscopy. Journal of Agricultural and Food Chemistry, 2003, 51(10):2896-2899.

[31]Schieber A, Keller P, Carle R. Determination of phenolic acids and flavonoids of apple and pear by high-performance liquid chromatography. Journal of Chromatography A, 2001, 910:265-273.

Related Articles 15

[1]	HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[2]	Xue BAI,Teng HUI,ZhenYu WANG,YunGang CAO,DeQuan ZHANG. Determination of 5 Nitropolycyclic Aromatic Hydrocarbons in Roasted Meat Products by High Performance Liquid Chromatography- Fluorescence Detection [J]. Scientia Agricultura Sinica, 2021, 54(5): 1055-1062.
[3]	WANG YaHui, LIU XiaoHong, YONG MingLi, XIONG AiSheng, SU XiaoJun. Analysis of Changes in Phenolic Acids of Luffa cylindrica Pulp During Browning Based on Metabolomics [J]. Scientia Agricultura Sinica, 2021, 54(22): 4869-4879.
[4]	ZHAO Shan,ZHONG LingLi,ZHOU Hong,LI Xi,LEI XinYu,HUANG ShiQun,ZHENG XingGuo,FENG JunYan,LEI ShaoRong,GUO LingAn. Identification and Analysis of Phenolic Acids in Rice Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. Scientia Agricultura Sinica, 2020, 53(3): 612-631.
[5]	ZOU YanYu,FANG Yong,LI Peng,HUANG QinQin,XIA Ji,LIU Qin,XIE MinHao,HU QiuHui. Comparative Analysis of Alkylresorcinols Homologue Composition in Whole Wheat Flour and Refined Wheat Flour [J]. Scientia Agricultura Sinica, 2020, 53(10): 2055-2065.
[6]	MA Wen,LIU Jiao,ZHANG XueYao,SHEN GuoHua,QIN XueMei,ZHANG JianQin. Enzymatic Characteristics and Metabolic Analysis to Malathion and p,p’-DDT of LmGSTS2 from Locusta migratoria [J]. Scientia Agricultura Sinica, 2019, 52(8): 1389-1399.
[7]	YuanYuan ZHU,Yuan XU,XingQi ZOU,YanLi YANG,LiLi LIU,JianQing WAN,Cui LI,Lu XU,QianYi ZHANG,YingJu XIA,Zhao WANG,HongWu LANG,Qin WANG,SongPing ZHANG,QiZu ZHAO. Determination of 146S Antigen in Inactivated Foot-and-Mouth Disease Vaccine by Size-Exclusion High-performance Liquid Chromatography and Quality Evaluation of Vaccine [J]. Scientia Agricultura Sinica, 2019, 52(20): 3695-3704.
[8]	XIAO ZhiMing, WANG Jun, SUO DeCheng, WEI ShuLin, JIA Zheng, LIU ChengXin, FAN Xia. Quantitative Determination of Diludine in Animal Feeds by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. Scientia Agricultura Sinica, 2018, 51(9): 1806-1814.
[9]	ZHAO XiJuan, ZHAO WuJi, XU HuaChao. Analysis of the Fingerprints of Different Orange Varieties and Their Differential Metabolites Based on Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry and Progenesis QI [J]. Scientia Agricultura Sinica, 2018, 51(13): 2551-2560.
[10]	SU Fan, XUE Jia, YANG Xi, DENG Hong, MENG YongHong, GUO YuRong. Effects of Phenolic Acids on Copigmentation and Stability of Anthocyanins in Red-Fleshed Apple [J]. Scientia Agricultura Sinica, 2017, 50(4): 732-742.
[11]	PING Hua, LI Yang, LI BingRu, HE ZhaoYing, LIU JiPei, MA ZhiHong. Simultaneous Determination of Multi Herbicides Residues in Vegetables by Dispersive Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. Scientia Agricultura Sinica, 2017, 50(21): 4159-4169.
[12]	YIN Cheng-miao, HU Yan-li, WANG Gong-shuai, ZHANG Xian-fu, ZHOU Hui, SHEN Xiang, CHEN Xue-sen, MAO Zhi-quan. Effect of Main Phenolic Acids of the Apple Replanted Soil on the Roots of Malus hupehensis Rehd. Seedlings [J]. Scientia Agricultura Sinica, 2016, 49(5): 961-969.
[13]	LIU Shuo, LIU You-chun, LIU Ning, ZHANG Yu-ping, ZHANG Qiu-ping, XU Ming, ZHANG Yu-jun, LIU Wei-sheng. Sugar and Organic Acid Components in Fruits of Plum Cultivar Resources of Genus Prunus [J]. Scientia Agricultura Sinica, 2016, 49(16): 3188-3198.
[14]	ZHANG Na, WANG Guo-xiang, Abacar Jose Daniel, LIU Zheng-hui, DING Cheng-qiang,TANG She, LI Gang-hua, WANG Shao-hua, DING Yan-feng. Determination of Phenolic Acids in Rice by Ultra-High Performance Liquid Chromatography [J]. Scientia Agricultura Sinica, 2015, 48(9): 1718-1726.
[15]	HU Hai-Tao-1, JIN Xiao-Qin-1, CHENG Long-Ping-1, YANG Li-1, CHEN Jian-Hua-1, FAN Ru-Chang-2, YANG Ling-1. Determination of Carotenoids in the Fruit of Elaeagnus conferta Roxb. by High Performance Liquid Chromatography [J]. Scientia Agricultura Sinica, 2014, 47(8): 1652-1656.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

A New Method for Analysis of Phenolic Acids in the Soil —Soil from Replanted Apple Orchards was Investigated

PDF