Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (15): 3212-3219.doi: 10.3864/j.issn.0578-1752.2013.15.016

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

The Apoptosis of Bovine Primary Mammary Epithelial Cells Induced by Staphylococcus aureus

 CUI  Xin-Jie, HU  Qing-Liang, LI  Yi-Ping, TAO  Lin, XIU  Lei, LIU  Bing-Chun, CHEN  Yuan, WANG  Xiao   

  1. College of Life Sciences, Inner Mongolia University, Hohhot 010010
  • Received:2013-02-01 Online:2013-08-01 Published:2013-05-20

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Abstract: 【Objective】 The objective of this experiment is to observe if apoptosis of the primary bovine mammary epithelial cells could be induced by Staphylococcus aureus in vitro. 【Method】Bovine Mammary Epithelial Cells were separated from fresh milk and evaluated by RT-PCR and protein assaies of keratin8. After infection, light microscopy, scanning electron microscopy and Annexin V/PI staining were used to evaluate the apoptosis of bovine mammary epithelial cells, and the apoptosis ratios were calculated. The expression of caspase 3 and caspase 8 were detected by RT-PCR. 【Result】 The cells’ shape looked like typical paving stones and keratin 8 was also detected by immunohistochemistry and PCR. Bovine mammary epithelial cells were round shrink aged with the apoptosis morphologies, and the expression of caspase 3 and caspase 8 was up-regulated after infection. 【Conclusion】S. aureus could induce apoptosis of bovine mammary epithelial cells, and the apoptosis pathway could be associated with caspase 3 and caspase 8.

Key words: S. aureus , bovine mammary epithelial cells , apoptosis

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