Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (12): 2615-2624.doi: 10.3864/j.issn.0578-1752.2013.12.024

• RESEARCH NOTES • Previous Articles    

Effect of Different Ripening Stages on Antioxidant Activity of Soluble Extracts from Mozzarella Cheese with Various Strains

 MA  Ling, PENG  Deng-Feng, LI  Hui, WANG  Xiao-Wen, LIU  Hui-Ping   

  1. 1.College of Food Science and Technology, Shanxi Agricultural University, Taigu 030801, Shanxi
    2.College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457
  • Received:2012-12-02 Online:2013-06-15 Published:2013-04-02

Abstract: 【Objective】 This study was conducted to examine the antioxidant activity of extracts with sterile water, pH 4.6 acetic acid buffer solution and 12% TCA for Mozzarella cheese in different mature periods in order to investigate the proper ripening periods for eating from the facet of antioxidant activity.【Method】Extracts of different ripening periods of Mozzarella cheese with combined starter were prepared and their scavenging DPPH capacity, reduction power, scavenging hydroxyl radical and superoxide anion were determined by using sterile water, pH 4.6 acetic acid buffer solution and 12% TCA as extraction solvent, respectively. 【Result】The results showed that soluble extracts had a certain antioxidant capacity. In the ripening of 50 days, most of the antioxidant indexes of various extracts from Mozzarella cheese with starters AB, AB+LPC, AB+BI were higher than in other ripening days, but cheese with AB+LA showed higher antioxidant activity both in the ripening of 40 d and 50 d. The proper eating stage was various with distinctive starters, for Mozzarella cheese with AB, AB+LPC and AB+BI starter, at the age of 50 d, while AB+LA between 40 and 50 days of ripening. 【Conclusion】 Antioxidant activity was various with different extracts, peptide contents in the extracts of 12% TCA were the highest. The proper mature stage for eating of Mozzarella cheese was different with different starters in consideration of the antioxidant activity.

Key words: Mozzarella cheese , strains , ripening periods , extracts , antioxidant activity

[1]Farvin K H S, Baron C P, Nielsen N S, Otte J, Jacobsen C. Antioxidant activity of yoghurt peptides: Part 2 — Characterisation of peptide fractions. Food Chemistry, 2010, 123: 1090-1097.

[2]Gupta A, Mann B, Kumar R, Sangwan R B. Antioxidant activity of cheddar cheeses at different stages of ripening International Journal of Dairy Technology, 2009, 62(3): 339-347.

[3]Sakanaka S, Tachibana Y, Ishihara N, Juneja L R. Antioxidant properities of casein calcium peptides and their effects on lipid oxidation in beef homogenates. Journal of Agricultural and Food Chemistry, 2005, 53: 464-468.

[4]董晓斌, 付丽丽, 朴姗善, 王昕, 侯聚敏. 发酵乳清蛋白制取抗氧化肽的研究. 食品科学, 2011, 32(增刊): 172-175.

Dong X B,Fu L L,Piao S S,Wang X, Hou J M. Study on the anti-oxidative capability of the fermentation product of the whey protein by probiotics. Food Science, 2001, 32(Suppl.): 172-175.(in Chinese)

[5]毛学英, 吴思嘉, 范金波, 徐世平, 王晶, 谷俊男, 任发政. 牦牛乳酪蛋白酶解产物清除DPPH•自由基活性分析. 现代食品科技, 2007, 24(7): 624-626.

Mao X Y, Wu S J, Fan J B, Xu S P, Wang J, Gu J N, Ren F Z. Scavenging activity of enzymatic hydrolysate of yak casein on DPPH radical. Modern Food Science and Technology, 2007, 24(7): 624-626. (in Chinese)

[6]郁晓盼, 赵肖蓉, 牟光庆. 酪蛋白抗氧化肽的制备及初步分离. 食品科技, 2011, 36(5): 29-31.

Yu X P, Zhao X R, Mou G Q. Preparation and preliminary separation of antioxidant peptide from casein. Food Technology, 2011, 36(5): 29-31. (in Chinese)

[7]Pritchard S R,  Phillips M,  Kailasapathy K. Identification of bioactive peptides in commercial cheddar cheese. Food Research International, 2010, 43: 1545-1548.

[8]Rizzello C G, Losito I, Gobbetti M, Carbonara T, Bari M D, Zambonin P G. Antibacterial activitied of peptides from water-soluble extracts of Italian cheese varieties. Journal of Dairy Science, 2005, 88: 2348-2360.

[9]Meira S M M, Daroit D J, Helfer V E, Corrêa A P F, Segalin J, Carro S, Brandelli A. Bioactive peptides in water-soluble extracts of ovine cheeses from southern Brazil and Uruguay. Food Research International, 2012, 48: 322-329.

[10]刘会平, 南庆贤, 马长伟. Mozzarella干酪成熟中蛋白水解与功能特性的变化. 农业工程学报, 2006, 22(6): 182-187.

Liu H P, Nan Q X, Ma C W. Changes of proteolysis and functional properties during ripening of Mozzarella cheese. Transactions of the CSAE, 2006, 22(6):182-187.(in chinese)

[11]Kilcawley K N, Nongonierma A B, Hannon J A, Doolan I A, Wilkinson M G. Evaluation of commercial enzyme systems to accelerate Cheddar cheese ripening. International Dairy Journal, 2012, 26: 50-57.

[12]Dimitrellou D, Kandylis P, Mallouchos A, Komaitis M, Koutinas A A, Kourkoutas Y. Effect of freeze–dried kefir culture on proteolysis in feta-type and whey-cheeses. Food Chemistry, 2010,119: 795-800.

[13]Yamaguchi T, Takamura H, Matoba T A, Terao J. HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Bioscience, Biotechnology, Biochemistry, 1998, 62(6): 1201-1204.

[14]吕喜茹, 郭亮, 常明昌, 李振, 王国晋, 冯翠萍. 姬松茸粗多糖抗氧化作用. 食用菌学报, 2010,17(1): 69-71.

Lü X R, Guo L, Chang M C, Li Z, Wang G J, Feng C P. Antioxidant activity of polysaccharide from Agaricus blazei Murill. Journal of Edible Fungi, 2010, 17 (1): 69-71. (in Chinese)

[15]王辉. 绞股蓝乙酸乙酯提取物有效成分的分离及生物活性研究[D]. 山西太谷: 山西农业大学, 2010.

Wang H. Research of separation and bioactivity for effective ingredients extracted with ethyl acetate from gynostemma[D]. Taigu, Shanxi: Shanxi Agricultural University, 2010. (in Chinese)

[16]陈伟. 不同方法测定玫瑰花红色素抗氧化性. 食品科学, 2008, 29(1): 273-275.

Chen W. Determine of different ways of antioxidant of red rose pigment. Food Science, 2008, 29(1): 273-275.(in Chinese)

[17]林燕, 王鸿飞, 李和生, 刘飞, 毛方华. 鱿鱼皮酶解产物的功能特性. 中国食品学报, 2011,11(5): 83-88.

Lin Y, Wang H F, Li H S, Liu F, Mao F H. Functional properties of enzymatic hydrolysates from squid skin. Journal of Chinese Institute of Food Science and Technology, 2011,11(5): 83-88. (in Chinese)

[18]王晓闻, 张鲁, 任石涛. 酸浆宿萼抗氧化活性研究. 中国食品学报, 2010,10(4): 136-140.

Wang X W, Zhang L, Ren S T. Studies on the antioxidant activity of Physalis alkekengi Calyx. Journal of Chinese Institute of Food Science And Technology, 2010,10(4):136-140. (in Chinese)

[19]张艳萍, 戴志远, 张虹. 贻贝蛋白的酶解及其酶解物的抗氧化活性研究.中国食品学报, 2011,12(1):10-18.

Zhang Y P, Dai Z Y, Zhang H. Enzymatic hydrolysis of mussel (Mytilus edulis) protein and antioxidant activity of hydrolysates. Journal of Chinese Institute of Food Science and Technology, 2011, 12(1): 10-18. (in Chinese)

[20]鲁伟, 任国谱, 宋俊梅. 蛋白水解液中多肽含量的测定方法. 食品科学, 2005, 26(7):169-171.

Lu W, Ren G P, Song J M. Determination of content of peptides in protein hydrolysates. Food Science, 2005, 26(7):169-171.(in Chinese )

[21]丁利君, 黄小梅, 何颖基. 罗非鱼蛋白制取多肽酶解条件的优化. 食品与机械, 2008, 24(2): 15-18.

Ding L J, Huang X M, He Y J. Optimization of hydrolysis conditions for peptides producing from tilapia protein. Food Machinery, 2008, 24(2):15-18. (in Chinese)

[22]Virtanen T, Pihlanto A, Akkanen S, Korhonen. Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria. Journal of Applied Microbiology, 2006, 102: 106-115.

[23]Phanturat P, Benjakul S, Visessanguan W, Roytrakul S. Use of pyloric caeca extract from bigeye snapper(Priacanthus macracanthus) for the production of gelatin hydrolysate with antioxidant activity. LWT-Food Science and Technology, 2010, 43: 86-97. 

[24]Silva S V, Pihlanto A, Malcata F X. Bioactivity peptides in ovine and caprine cheese like systems prepared with proteases from Cynara cardunculus. Journal of Dairy Science, 2006, 89: 3336-3344.

[25]霍湘, 王安利, 杨建梅. 含硫氨基酸的抗氧化作用. 生物学通报, 2006, 41(4): 3-4.

Huo X, Wang A L, Yang J M. The antioxidant activity of sulfur-containing animo acids. Bulletin of Biology, 2006, 41(4):3-4. (in Chinese)

[26]丁晓雯, 李洪军, 章道明. 抗氧化肽研究进展. 食品研究与开发, 2003, 24(3): 36-38.

Ding X W, Li H J, Zhang D M. Research of the progress of antioxidant peptide. Food Research and Development, 2003, 24(3): 36-38. (in Chinese)

[27]刁静静, 孔保华, 刁新平. 骨蛋白水解物抗氧化活性及其作用模 式. 中国农业科学, 2009, 42(1): 238-244.

Diao J J, Kong B H, Diao X P. Antioxidant activity and the possible mode of action of bone protein hydrolysates. Scientia Agricultura Sinica, 2009, 42(1): 238-244. (in Chinese)
[1] LIU WangJing,TANG DeFu,AO ChangJin. Effect of Allium mongolicum Regel and Its Extracts on the Growth Performance, Carcass Characteristics, Meat Quality and Serum Biochemical Indices of Captive Small-Tailed Han Sheep [J]. Scientia Agricultura Sinica, 2022, 55(17): 3461-3472.
[2] HOU ChengLi,HUANG CaiYan,ZHENG XiaoChun,LIU WeiHua,YANG Qi,ZHANG DeQuan. Changes of Antioxidant Activity and Its Possible Mechanism in Tan Sheep Meat in Different Postmortem Time [J]. Scientia Agricultura Sinica, 2021, 54(23): 5110-5124.
[3] ZHAO Shan,ZHONG LingLi,QIN Lin,HUANG ShiQun,LI Xi,ZHENG XingGuo,LEI XinYu,LEI ShaoRong,GUO LingAn,FENG JunYan. Effects of Different Drying Methods on Functional Components and Antioxidant Activity in Sweet Potato Leaves [J]. Scientia Agricultura Sinica, 2021, 54(21): 4650-4663.
[4] LI Jie,JIA XuChao,ZHANG RuiFen,LIU Lei,CHI JianWei,HUANG Fei,DONG LiHong,ZHANG MingWei. Isolation, Structural Characterization and Antioxidant Activity of Black Sesame Melanin [J]. Scientia Agricultura Sinica, 2020, 53(12): 2477-2492.
[5] ZhengKe WU,GuoHua LIU,Yang LI,AiJuan ZHENG,WenHuan CHANG,ZhiMin CHEN,HuiYi CAI. Optimization of Solid State Fermentation for Rapeseed Meal with Mixed Strains [J]. Scientia Agricultura Sinica, 2019, 52(24): 4603-4612.
[6] WANG LiFang,ZHANG XingFu. Effects of Artemisia annua Extracts on CLA Synthesis and Mechanism [J]. Scientia Agricultura Sinica, 2019, 52(18): 3271-3278.
[7] YU Jing,ZHANG WeiXing,MA LanTing,XU BaoHua. Effect of Dietary α-Linolenic Acid Levels on Physiological Function of Apis mellifera ligustica Worker Bee Larvae [J]. Scientia Agricultura Sinica, 2019, 52(13): 2368-2378.
[8] WANG LiFang, ,AO ChangJin. The Effects of Artemisia annua Extracts on the Rumen Fermentation in Dairy Cows [J]. Scientia Agricultura Sinica, 2018, 51(23): 4548-4555.
[9] LI XiaoYing, XUE Mei, FAN WenQiao, LUO Jie. Analysis of Phenolic Compounds and Antioxidant Activities of Blueberry Leaves from Different Drying Methods [J]. Scientia Agricultura Sinica, 2018, 51(13): 2570-2578.
[10] LIU Yu, LIU ShengYu, LU JuanFang, YU QingFan, XI WanPeng. Evaluation of Flavor Quality and Antioxidant Capacity of Apple Fruits from Three Xinjiang Red-Flesh Lines [J]. Scientia Agricultura Sinica, 2017, 50(8): 1495-1504.
[11] DONG Li-hong, ZHANG Rui-fen, XIAO Juan, DENG Yuan-yuan, ZHANG Yan, LIU Lei, HUANG Fei, WEI Zhen-cheng, ZHANG Ming-wei. Separation and Antioxidant Activity of Different Phenolic Compound Fractions from Litchi Pulp [J]. Scientia Agricultura Sinica, 2016, 49(20): 4004-4015.
[12] NI Wei-ru, WANG An-ran, HE Xi-yan, XU Jin, WU Shu-jing, CHEN Xue-sen, MAO Zhi-quan, SHEN Xiang. Effects of After-Reap Soil on Physiological Indexes and Leaf Antioxidant Activity of the Different Apple Cultivars With the Same Rootstock [J]. Scientia Agricultura Sinica, 2016, 49(18): 3597-3607.
[13] LAI Ting, LIU Lei, ZHANG Ming-wei, ZHANG Rui-fen, ZHANG Yan, WEI Zhen-cheng, DENG Yuan-yuan. Effect of Lactic Acid Bacteria Fermentation on Phenolic Profiles and Antioxidant Activity of Dried Longan Flesh [J]. Scientia Agricultura Sinica, 2016, 49(10): 1979-1989.
[14] HU Li, WANG Jin-zhi, ZHANG Chun-hui, TANG Chun-hong, DU Gui-hong, LI Xia, LI Chun-hong. Characteristics of Maillard Reaction Products from Enzymatic Hydrolysate of Chicken Bone Extract as Influenced by pH and Temperature [J]. Scientia Agricultura Sinica, 2015, 48(18): 3689-3700.
[15] YIN Cheng-Miao, ZHANG Xian-Fu, HU Yan-Li, SHEN Xiang, CHEN Xue-Sen, WU Shu-Jing, MAO Zhi-Quan. Effect of Different Concentrations of Organic Matter Fermentation Fluid on the Young Apple Tree Leaf Photosynthesis Fluorescent Parameters and Root Antioxidant Activity Under Replant Conditions [J]. Scientia Agricultura Sinica, 2014, 47(9): 1847-1857.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!