Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (10): 2041-2049.doi: 10.3864/j.issn.0578-1752.2013.10.009

• PLANT PROTECTION • Previous Articles     Next Articles

Effects of Nicotine on Feeding Behavior of Bemisia tabaci B and Q Biotypes Based on EPG and Liquid Diet Sac Technique

 LI  Xiao-Min, LI  Jing-Jing, TANG  Qing-Bo, YAN  Feng-Ming   

  1. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002
  • Received:2013-01-08 Online:2013-05-15 Published:2013-04-01

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Abstract: 【Objective】 The effects of nicotine on the feeding behaviors of the Bemisia tabaci B and Q biotypes were investigated, with the aim of establishing a corresponding evaluating system based on the electrical penetration graph (EPG) and the liquid diet sac (LDS) technique. 【Method】 EPG method combined with a LDS was used to quantify the effect of the concentrations of nicotine on the feeding behaviors of the B. tabaci. 【Result】 Relative amplitudes and frequencies in EPG waveforms of the B. tabaci in 10% sucrose solution, compared to those in 5% and 15% sucrose solutions, were analogous to the waveforms of B. tabaci on plants. The durations of ingestion waveforms decreased with the increase of nicotine levels in 10% sucrose solution. Salivation (E1) and passive ingestion (E2) durations were reduced and the penetration duration was prolonged even at the low concentration (0.001%) of nicotine. The passive ingestion from LDS was completely inhibited at a higher concentration of nicotine (0.01%). Although the major EPG pathway variables between B. tabaci B and Q biotypes were similar, the duration of passive ingestion of Q biotype was significantly longer than that of the B biotype on the diet without nicotine or with lower nicotine concentration. 【Conclusion】 The valuable EPG variables for EPG+LDS system include the durations of C, E1 and E2 patterns. Ten percent sucrose solution in LDS was more suitable for recording the feeding behaviors of B. tabaci using EPG technique. The feeding behaviors of B. tabaci B and Q biotypes were signi?cantly inhibited by nicotine, but Q biotype had higher nicotine adaptability than B biotype.

Key words: Bemisia tabaci , liquid diet sac (LDS) , electrical penetration graph (EPG) , nicotine , feeding behaviors

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