Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (10): 2014-2021.doi: 10.3864/j.issn.0578-1752.2013.10.006

• PLANT PROTECTION • Previous Articles     Next Articles

Identification of the Lipopeptides from Bacillus amyloliquefaciens Lx-11 and Biocontrol Efficacy of Surfactin Against Bacterial Leaf Streak

 ZHANG  Rong-Sheng, WANG  Xiao-Yu, LUO  Chu-Ping, LIU  Yong-Feng, LIU  You-Zhou, CHEN  Zhi-Yi   

  1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
  • Received:2013-01-08 Online:2013-05-15 Published:2013-03-28

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Abstract: 【Objective】The objective of this study is to isolate and identify the lipopeptides produced by Bacillus amyloliquefaciens Lx-11 and their biocontrol efficacy against bacterial leaf streak (BLS). 【Method】 Three primers designed based on the known lipopeptide genes were used to amplify the corresponding genes from the genome of Lx-11 strain. Lipopeptides were identified by MALDI-TOF-MS techniques.【Result】The PCR products with three primer pairs were cloned and sequenced. The BLAST analysis showed that the sfp, fenB, ituA or bamA genes exist in the genome of B. amyloliquefaciens Lx-11. Three kinds of lipopeptides surfactin, bacillomycin D and fengycin were identified in the antimicrobial extract by the mass spectrum analysis. The antibacterial activity could be associated with surfactin-lipopeptides, and practically abolished in surfactin-deficient mutants. Lx-11 strain significantly reduced disease incidence of BLS.【Conclusion】The antibacterial activity of the surfactin- deficient mutant strain was practically abolished, which suggested that surfactin might play an important role in control of BLS.

Key words: Bacillus amyloliquefaciens , bacterial leaf streak (BLS) , lipopeptides , biological control

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