Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (5): 1044-1053.doi: 10.3864/j.issn.0578-1752.2013.05.020

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Isolation and Identification of Tembusu Virus Strain SHYG from Goose

 PAN  Jin-Jin, ZOU  Xiao-Yan, LI  Xin, SU  Chun-Hu, CHAI  Mao, JIANG  Yi, HU  Yan-Fen, ZHAO  Guo, WANG  Yan-Hong, SHI  Huo-Ying, CHEN  Su-Juan, PENG  Da-Xin   

  1. 1.Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, Jiangsu
    2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu
  • Received:2012-02-24 Online:2013-03-01 Published:2013-01-17

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Abstract: 【Objective】 The objective of the study is to isolate Tembusu virus from goose and explore its pathogenicity to young goose.【Method】RT-PCR was applied to detect Tembusu virus in sick geese samples collected from three farms in Jiangsu, in which the positive samples were used to isolate Tembusu virus. The biological characteristics and sequence of Envelope (E) gene of isolated virus were determined.【Result】These samples were all PCR positive for Tembusu virus. One strain was isolated from a positive sample and named as SHYG. Sequence analysis based on E gene suggested that its sequence was homologous highly with that of Tembusu viruses isolated from ducks in China. The SHYG strain induced cytopathic effect in Vero cell. The results of animal experiment revealed that the SHYG strain caused depression, diarrhea, neural symptom, and even death when it was inoculated to 2-week-old geese, but no pathogenic to mice. The histological observation showed congestion, bile capillary expansion and steatosis in liver, hemorrhage, inflammatory exudate and hemosiderin pigmentation in lung, reticuloendothelial cell vacuolization in spleen, hemorrhage in kidney, and hyperemia and proliferation of gliocyte in brain. 【Conclusion】 The Tembusu virus strain SHYG is pathogenic to young goose.

Key words: goose , Tembusu virus , pathogenic

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