Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (1): 45-53.doi: 10.3864/j.issn.0578-1752.2013.01.006

• PLANT PROTECTION • Previous Articles     Next Articles

NSvc4 and CP Proteins Contribute to the Pathogenicity of Rice Stripe Virus

 YUAN  Zheng-Jie, JIA  Dong-Sheng, WU  Zu-Jian, WEI  Tai-Yun, XIE  Lian-Hui   

  1. Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002
  • Received:2012-05-07 Online:2013-01-01 Published:2012-08-08

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Abstract: 【Objective】 The objective of this study is to investigate the roles of NSvc4 and CP proteins of Rice stripe virus (RSV) in pathogenicity to reveal the mechanisms underlying RSV pathogenesis. 【Method】 The subcellular localization patterns of six RSV proteins were studied by expressing them in epidermal cells of Nicotiana benthamiana through Agrobacterium inoculation. The interaction between NSvc4 and five other proteins of RSV was investigated using Bimolecular fluorescence complementation (BiFC), and the interaction between NSvc4 and CP was further confirmed by yeast two-hybrid experiments. The possible involvement of NSvc4 and CP in pathogenicity was exploited by expressing them individually or together in N. benthamiana employing the vector Potato virus X (PVX). 【Result】 NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N. benthamiana when co-expressed, both NSvc4 and CP could induce leaf mosaic symptoms when they were expressed using PVX. However, leaf mosaic symptoms caused by CP was recovered in systemic leaves of PVX inoculated plants. When expressed together or as a fused protein using PVX, NSvc4 and CP induced severe symptoms in both inoculated and systemic leaves of N. benthamiana. Real-time PCR experiments showed that NSvc4 and CP had no effects on the accumulation of PVX, and the severity of the symptoms induced by the recombinant virus correlated with the expression levels of NSvc4 and CP. 【Conclusion】 Both NSvc4 and CP have pathogenicity properties and they might have synergistical function in RSV pathogenesis.

Key words: Rice stripe virus , NSvc4 and CP protein , pathogenicity , Nicotiana benthamiana

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