Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (18): 3892-3898.doi: 10.3864/j.issn.0578-1752.2012.18.022

• RESEARCH NOTES • Previous Articles    

Cloning and Characteristics Analysis of Chemosensory Protein Gene BtabCSP1 in Bemisia tabaci Q Biotype

 BAI  Run-E, LI  Jing-Jing, TANG  Ya-Fei, XIONG  Da-Bin, LI  Shuai-Liang, YAN  Feng-Ming   

  1. 1.河南农业大学植物保护学院，郑州450002
  • Received:2011-11-22 Online:2012-09-15 Published:2012-02-22

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Abstract: 【Objective】 The objective of this study is to clone the full length cDNA of the gene encoding chemosensory proteins from Bemisia tabaci Q biotype, and to analyze its expression pattern at different developmental stages.【Method】Based on the amino acid sequence of aphids (Myzus persicae) chemosensory proteins OS-D2a (ABM5558) reported previously, a pair of primers were designed based on simulation of gene splicing and RT-PCR was used to clone the chemosensory protein gene BtabCSP1. 【Result】Sequencing and structural analysis showed that BtabCSP1 contains a 2 626 bp long complete reading frame (with 2 exons and 1 intron) and encodes 131 amino acid residues with a CX6CX18CX2 domain, the typical characteristics of a chemosensory protein gene. As displayed by semi-quantitative RT-PCR amplification from total RNA of B. tabaci of various stages, BtabCSP1 was expressed in all samples and the expressions were more apparent in the 2nd instar, 3rd instar and 4th instar (pupal stage) nymphs. The amino acid (encoded by BtabCSP1) sequence cluster analysis showed that B. tabaci was distantly related to fruit flies and aphids and chemosensory protein gene was active in the process of species evolution.【Conclusion】BtabCSP1 was isolated from B. tabaci Q biotype, and the expression pattern suggested the close relationship between the expression of this gene and the development of B. tabaci.

Key words: Bemisia tabaci Q biotype, CSP, gene cloning, characteristics analysis

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