Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (18): 3744-3754.doi: 10.3864/j.issn.0578-1752.2012.18.007

• PLANT PROTECTION • Previous Articles     Next Articles

Isolation, Screening and Identification of Bacterial Agents for Biological Control of Colorado Potato Beetle (Leptinotarsa decemlineata)

 LUO  Hua-Dong, YAN  Jia-Lin, YU  Yang, TAN  Wan-Zhong   

  1. 1.西南大学植物保护学院外来入侵生物研究中心，重庆 400716
  • Received:2011-12-29 Online:2012-09-15 Published:2012-05-10

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Abstract: 【Objective】 The objective of this study is to isolate and screen bacterial biocontrol agents against Colorado potato beetle (CPB) (Leptinotarsa decemlineata). 【Method】 Diseased CPB adult samples were collected from Xinjiang and bacterial isolates were obtained from the samples. These isolates were tested for CPB pathogenicity. Highly insecticidal isolates were screened through laboratory and field assays and identified morphologically and molecularly. 【Result】 A total of 126 bacterial isolates were obtained from diseased adults of CPBs collected from Xinjiang Autonomous Region of China and 36 isolates showed protein- and chitin-degrading activity. Among these isolates, 11 infected 1st instar and 2nd instar CPB larvae and resulted in lethal rates of 21.0%-77.9% within 7 days after inoculation when tested on detached potato leaves in the laboratory. The 11 infectious isolates were selected for field tests and 6 of them induced high CPB mortality rates. The CPB-lethal rates by the 3 isolates (CPB008, CPB012 and CPB016) reached 41.0%-49.9%. The other 3 isolates (CPB072, CPB108 and CPB111) also had significantly high CPB lethal rates (21.1%-37.8%). CPB008, CPB012, CPB016 and CPB111 were identified as Bacillus thuringiensis and CPB072, CPB108 were B. atrophaeus, respectively. 【Conclusion】 The screened 3 isolates (CPB008, CPB012 and CPB016) have strong CPB pathogenicity, and could be potential applications in biological control of this destructive pest in Xinjiang of China.

Key words: Colorado potato beetle, Bacillus thuringiensis, Bacillus atrophaeus, biological control, insecticidal efficiency

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