黍稷种质资源芽、苗期耐中性混合盐胁迫评价与耐盐生理机制研究

doi:10.3864/j.issn.0578-1752.2012.18.006

Abstract

Abstract: 【Objective】 Salt-tolerance and physiological responses of broomcorn millet (Panicum miliaceum L.) were evaluated under a range of NaCl+Na2SO4 concentrations to explore and utilize the germplasm with high salt-tolerance and to provide physiological indicators for identifying salt-tolerance at seedling stage. 【Method】 The growth parameters of 16 genotypes of P. miliaceum L. at germination and seedling stages were measured under various salt stresses, and the genotypes were sorted out using the method of membership function. The physiological characteristics of the genotypes in terms of concentration of proline and Na+/K+ were analyzed.【Result】It was shown that the relative germination value, survival seedling number, shoot length, root length and biomass of above and underground all decreased with the increase of salt concentration, although genotypes differed in the degrees of response to salt stress. Based on the membership function on the means of parameters at germination and seedling stages for each level of salt stress, the 16 genotypes were finally ranked with Zhongwei Dahuangmi, Ningmi No4 and 64-4129 as the most tolerant genotypes, and Bameng 573 Huangmizi, Yimen Yidianzong and Wahui Ruanmi as the least tolerant ones among all tested materials. Proline content in shoot increased in response to increased salt stress, with Zhongwei Dahuangmi and Ningmi No.4 had significantly higher proline content, while lower relative Na+/K+ than the others when salt concentrations at 160 and 200 mmol.L-1 (P＜0.05). 【Conclusion】Remarkable variations in salt tolerance were detected among the 16 genotypes of P. miliaceum L., which have provided a basis for the utilization of salt tolerant broomcorn millet genetic resources.

Key words: Panicum miliaceum L., mixed salt stress, salinity tolerance, physiological response

LIU Min-Xuan, ZHANG Zong-Wen, WU Bin, LU Ping. Evaluation of Mixed Salt-Tolerance at Germination Stage and Seedling Stage and the Related Physiological Characteristics of Panicum miliaceum L.[J].Scientia Agricultura Sinica, 2012, 45(18): 3733-3743.

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Evaluation of Mixed Salt-Tolerance at Germination Stage and Seedling Stage and the Related Physiological Characteristics of Panicum miliaceum L.

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