Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (17): 3669-3676.doi: 10.3864/j.issn.0578-1752.2012.17.026

• RESEARCH NOTES • Previous Articles    

Relaxant Effect of Sanguinarine on Ex vivo Intestine Smooth Muscle Movement in Rabbits

 WANG  Hui, YU  Chun-Hong, XU  Jian-Ping, WANG  Yue-Shang, LIU  Zhao-Ying, CHEN  Xiao-Jun, SUN  Zhi-Liang   

  1. 1.湖南农业大学动物医学院，长沙 410128
    2.山东省鲁南中药材资源开发工程技术研究中心，山东临沂276005
    3.山东华禧药业有限公司，山东禹城 251200
    4.湖南省兽药工程技术研究中心,长沙 410128
  • Received:2011-09-27 Online:2012-09-01 Published:2012-09-01

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Abstract: 【Objective】 The objective of this study was to investigate the relaxant effect of sanguinarine on intestinal smooth muscle contractions and the mechanisms involved. 【Method】 The experiments were performed in isolated intestinal smooth muscle of rabbits. Inhibitory effects of sanguinarine on contraction of normal intestinal smooth muscle and on that of the intestinal muscle treated with acetylcholine (ACh), phentolamine and Barium Chloride (BaCl2) were compared by analyzing the frequency and amplitude of contractions. 【Result】 Results showed that sanguinarine significantly inhibited the frequency and amplitude of spontaneous contraction of isolated intestinal smooth muscle in a dose-dependent manner. The intestinal smooth muscle contractions caused by Ach and Bacl2 was abolished by sanguinarine (0.4 mg•mL-1), and the frequency and amplitude of the intestinal smooth muscle contraction induced by phentolamine (α-receptor antagonist) decreased after incubation with sanguinarine. 【Conclusion】 These results suggest that the mechanism of relaxant effect of sanguinarine on intestinal smooth muscle contractions may be related to the blockage of M receptor by competing with ACh, to the excitement of α-receptor through competing with phentolamine and to the direct inhibit on of the motility of intestinal smooth muscle.

Key words: sanguinarine, rabbit, intestine smooth muscle, relaxation

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