Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (8): 1653-1660.doi: 10.3864/j.issn.0578-1752.2012.08.022

• RESEARCH NOTES • Previous Articles     Next Articles

Over-Expression of the Arabidopsis CBF1 Gene in Dongguandajiao (Musa spp.ABB group) and Detection of Its Cold Resistance

 LIU  Kai, HU  Chun-Hua, DU  Fa-Xiu, ZHANG  Yu-E, WEI  Yue-Rong, YI  Gan-Jun   

  1. 1.湖南农业大学园艺园林学院，长沙 410128
    2.广东省农业科学院果树研究所/农业部南亚热带果树生物学与遗传资源利用重点实验室，广州 510640
  • Received:2011-11-21 Online:2012-04-15 Published:2012-02-20

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Abstract: 【Objective】To elucidate the effects of over-expression of AtCBF1 gene on tolerance to low-temperature in Musa ABB cv. Dongguandajiao and establish a foundation for cloning cold resistance relative genes from Dongguandajiao.【Method】Transgenic plants transformed by AtCBF1 gene were generated based on Agrobacterium tumerficiens-mediated transformation of embryogenic cell suspensions (ECS) of Dongguandajiao. The transgenic plants were confirmed via GUS staining, PCR, RT-PCR and real-time fluorescence quantitative RT-PCR(RT-qPCR). The cold tolerance of transgenic plants were evaluated by the comparisons of plant morphology and physiological parameters such as chilling injury symptom, superoxide dismutase (SOD) activities, malonaldehyde (MDA) content and so on among the transgenic lines and non-transformed plants (CK) after low-temperature treatment.【Result】Six resistant lines were initiated in this experiment. Results from GUS staining showed that all resistant plants were positive except T1 line. PCR showed that AtCBF1 gene was detected in the six transformed lines, but gus gene not in T1 line. Expression of AtCBF1 gene in the six transgenic lines was confirmed using RT-PCR. Difference existed among T1, T2 and T3 for AtCBF1 expression in RT-qPCR analysis. After low-temperature stress treatment, the relative electric conductivity and MDA content of leaves of transgenic lines were lower than that of non-transformed plants. However, the superoxide dismutase (SOD) activities was higher in transgenic lines. The chilling injury symptom of leaves of transgenic lines were much slighter than that of the CK under low-temperature treatment.【Conclusion】 Over-expression of AtCBF1 gene increased SOD activities and decreased MDA content and electrolyte leakage rate caused by low -temperature. Thus the degree of over-oxidation of the cellular membrane is lessenned and the low-temperature stress tolerant capacities in transgenic plantain plants are improved.

Key words: Musa spp. ABB group, AtCBF1 gene, transgenic, cold resistance

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