Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (8): 1499-1504.doi: 10.3864/j.issn.0578-1752.2012.08.005

• PLANT PROTECTION • Previous Articles     Next Articles

Spatio-Temporal Localization Changes of Endogenous Hydrogen Peroxide During Cell Division Cycle of Xanthomonas oryzae pv. oryzae

 LI  Xin, LI  Xin-Ling, PANG  Xin-Yue, ZHU  Wen-Xue, FAN  Jin-Ling, LUO  Lei, DU  Lin, WANG  Na, WANG  Li-Ping   

  1. 1.河南科技大学食品与生物工程学院，河南洛阳 471003
    2.中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京100193
    3.河南科技大学医学技术与工程学院，河南洛阳 471003
  • Received:2011-08-25 Online:2012-04-15 Published:2011-09-20

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Abstract: 【Objective】The objective of this study is to investigate the changes of spatio-temporal localization of endogenous hydrogen peroxide (H2O2) in strains of Xanthomonas oryzae pv. oryzae, and to illustrate the physiological function of H2O2 in bacterial proliferation. 【Method】 Histochemical methods through transmission electron microscope were used to observe localization of endogenous H2O2. 【Result】 Even though the levels of endogenous H2O2 production were different among various strains, the produced H2O2 was localized in the cell wall of all Xanthomonas oryzae pv. oryzae strains tested. Interestingly, the accumulated H2O2 was also localized in the mesosome-like structure and nucleoids during the cell division cycle of strains. Furthermore, results revealed the quantitative and dimensional changes of H2O2 accumulation in the two additional locations. 【Conclusion】Additional locations of the accumulated H2O2 are closely associated with the process of cell division of all Xanthomonas oryzae pv. oryzae strains investigated. Spatio-temporal localization changes of endogenous H2O2 should be an ubiquitous phenomenon during cell division cycle of bacterium. H2O2 may play key roles in bacterial proliferation.

Key words: cell division, hydrogen peroxide, localization, mesosome-like structure, nucleoid, Xanthomonas oryzae pv. oryzae

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