Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (5): 966-972.doi: 10.3864/j.issn.0578-1752.2012.05.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Cloning and Analysis of MC1R Gene in Silky Fowl and Its Prokaryotic Expression

 CHI  Liang, LI  Lan, PAN  Qing-Jie, LIU  Huan-Qi   

  1. 1.青岛农业大学动物科技学院，山东青岛 266109
  • Received:2011-03-07 Online:2012-03-01 Published:2011-11-10

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Abstract: 【Objective】 MC1R (melanocortin 1-receptor) gene was analyzed and expressed in prokaryotic expression system.【Method】The CDs of silky fowl melanocortin 1-receptor gene was amplified and cloned from total RNA of silky fowl by RT-PCR according to the corresponding sequence in Gallus gallus. The biological information analysis was performed with it. The prokaryotic expression vector pET32a-MC1R was constructed and expressed in E. coli BL21 (DE3).【Result】The ORF of silky fowl MC1R gene consisted of 945 nucleotides and encoding 314 amino acids. The prokaryotic expression system of recombined vector pET32a-MC1R was constructed successfully and the protein MC1R was expressed in vitro by inducing with IPTG. 【Conclusion】 The MC1R gene of silky fowl was closed successfully and its relationship with melanin traits and its genetic relationship with other kinds of chicken were analyzed by constructing phylogenetic tree. At the same time, the MC1R protein of silky fowl was optimizedcy expressed in E. coli.

Key words: silky fowl, melanin, MC1R, prokaryotic expression

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