亚慢性镉暴露对五指山猪肝脏生化指标的影响

doi:10.3864/j.issn.0578-1752.2012.03.019

Abstract

Abstract: 【Objective】 The objective of this study was to look for a sensitive monitoring index of liver injury induced by cadmium and provide a scientific basis for cadmium monitoring and prevention by studying the influences of subchronic cadmium exposure on liver biochemical indexes.【Method】Twenty healthy 3-month-old Wuzhishan Pig (WZSP )were divided randomly into 5 groups, and then fed with basal diet containing 0.0 mg•kg-1 (groupⅠ), 0.5 mg•kg-1 (group Ⅱ), 2.0 mg•kg-1 (group Ⅲ), 8.0 mg•kg-1 (group Ⅳ), 32.0 mg•kg-1 (group Ⅴ) doses of cadmium respectively over one hundred days. Blood were collected every 20 days to determine the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyltransferase (γ-GGT). Blood cadmium and liver cadmium were determined at the end of experiment. 【Result】 Compared with group Ⅰ, ALT activity in groups Ⅱ and Ⅲ increased significantly after exposing to cadmium for 80 days (P＜0.05), followed by 60 days for group Ⅳ and 20 days for group Ⅴ but not within 40 days. AST activities in groups Ⅱ and Ⅲ increased significantly after exposing to cadmium for 20 days (P＜0.05) but not within 40 days (P＞0.05), while groups Ⅳ and Ⅴ increased significantly within 100 days (P＜0.05). GGT activities in groups Ⅱ, Ⅳ, and Ⅴ all increased significantly after exposing to cadmium for 100 days (P＜0.05). Liver cadmium and blood cadmium in groups Ⅳ and Ⅴ increased significantly compared to group Ⅰ(P＜0.05). There was a significant correlation among cadmium exposure, blood cadmium and liver cadmium at the 0.01 level, ALT activity was significantly correlated with cadmium exposure and liver cadmium at the 0.05 level. 【Conclusion】 Liver injury can be induced by subchronic cadmium exposure at concentrations higher than 0.5 mg•kg-1. Serum ALT activity and blood cadmium can be used respectively as a liver effective biomarker and exposure biomarker of liver injury induced by subchronic cadmium exposure.

Key words: Wuzhishan Pig, cadmium, liver, subchronic toxicity

WU Xiao-Sheng, WEI Shuai, WEI Yi-Min, GUO Bo-Li, YANG Ming-Qi, LIU Xiao-Ling. Effect of Sub-chronic Cadmium Exposure on Liver Biochemical Index in Wuzhishan Pig[J].Scientia Agricultura Sinica, 2012, 45(3): 562-568.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2012.03.019

https://www.chinaagrisci.com/EN/Y2012/V45/I3/562

References

[1]Satarug S, Baker J R, Urbenjapol S, Haswell-Elkins M, Reilly P E, Williams D J, Moore M R. A global perspective on cadmium pollution and toxicity in non-occupationally exposed population. Toxicology Letters, 2003, 137: 65-83.

[2]Seixas T G, Kehrig H A, Fillmann G, Di Beneditto A P, Souza C M, Secchi E R, Moreira I, Malm O. Ecological and biological determinants of trace elements accumulation in liver and kidney of pontoporia blainvillei. Science of the Total Environment, 2007, 385: 208-220.

[3]Malik N, Biswas A K, Qureshi T A, Borana K, Virha R. Bioaccumulation of heavy metals in fish tissues of a freshwater lake of Bhopal. Environmental Monitoring Assessment, 2010, 160(4): 267-276.

[4]Roels H, Bernard A M, Cardenas A, Buchet J P, Lauwerys R R, Hotter G, Ramis I, Mutti A, Franchini I, Bundschuh I, Stolte H, De Broe M E, Nuyts G D, Taylor S A, Price R G. Markers of early renal changes induced by industrial pollutants. Ⅲ application to workers exposed to cadmium. British Journal of Industrial Medicine, 1993, 50: 37-38.

[5]Nordberg G F, Nogawa K, Nordberg M, Friberg L T. Cadmium// Nordberg G F, FowlerB A, Nordberg M, Friberg L T. Handbook on the Toxicology of Metals. 3rd edition. Amsterdam and Boston: Academic Press, 2007: 446-486.

[6]冯书堂, 褚武军, 王雅春. 中国五指山猪. 北京: 中国农业科技出版社, 1999: 30-50.

Feng S T, Chu W J, Wang Y C. China Wuzhishan Pig. Beijing: China Agricultural Science and Technology Press, 1999: 30-50. (in Chinese)

[7]Eklund G, Linden A, Tallkvist J, Oskarsson A. Bioavailability of cadmium from in vitro digested infant food studied in Caco-2 cells. Journal of Agricultural and Food Chemistry, 2003, 51(14): 4168-4174.

[8]Tzirogiannis K N, Panoutsopoulos G I, Demonakou M D, Papadimas G K, Kondyli V G, Kourentzi K T, Hereti R I, Mykoniatis M G. The hepatoprotective effect of putrescine against cadmium-induced acute liver injury. Archive Toxicology, 2004, 78(6): 321-329.

[9]Dudley R E, Svoboda D J, Klaassen C D. Acute exposure to cadmium causes severe liver injury in rats. Toxicology and Applied Pharmacology, 1982, 65: 302-313.

[10]Sani M J, Mahmoudabady A Z, Pur M A, Sharifabady R R, Bahadoran H, Jafari A, Rezavand B, Mohseni A, Montazeri S. The cytotoxicity and genotoxicity effect of cadmium on mice liver. Toxicology Letters, 2009, 189: S141.

[11]Renugadevi J, Prabu S M. Cadmium-induced hepatotoxicity in rats and the protective effect of naringenin. Expermental and Toxicologic Pathology, 2010, 62: 171-181.

[12]任文华, 陈龙, 谷宇, 高伟, 朱善良. 慢性镉暴露诱发大鼠肝脏几种酶活性的变化. 南京师大学报: 自然科学版, 2002, 25(4): 91-94.

Ren W H, Chen L, Gu Y, Gao W, Zhu S L. Change of some liver enzyme activities in rats induced by chronic cadmium-exposure. Journal of Nanjing Normal University: Nature Science, 2002, 25(4): 91-94. (in Chinese)

[13]GB/T 13078-2001, 饲料卫生标准. 北京: 中华人民共和国国家质量监督检验检疫局, 2001.

GB/T13078-2001, Hygienical Standard for Feeds. Beijing: General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, 2001. (in Chinese)

[14]严卫星, 丁晓雯. 食品毒理学. 北京: 中国农业大学出版社, 2009: 299.

Yan W X, Ding X W. Food Toxicology. Beijing: China Agricultural University Press, 2009: 299. (in Chinese)

[15]Thijssen S, Maringwa J, Faes C, Lambrichts I, Kerkhove E V. Chronic exposure of mice to environmentally relevant, low doses of cadmium leads to early renal damage, not predicted by blood or urine cadmium levels. Toxicology, 2007(229): 145-156.

[16]Shaikh Z A, Smith L M. Biological indicators of cadmium exposure and toxicity. Cellular and Molecular Life Sciences, 1984, 40: 36-43.

[17]金泰廙, 孔庆瑚, 叶葶葶, 王洪复, 蒋学之, 汪再娟, 朱国英, 吴训伟, 曾祥斌, 薄梅花. 镉致人体健康损害的环境流行病学研究. 环境与职业医学, 2002, 19(1): 10-20.

Jin T Y, Kong Q H, Ye T T, Wang H F, Jiang X Z, Wang Z J, Zhu G Y, Wu X W, Zeng X B, Bo M H. Environmental epidemiology: human health impacts caused by cadmium. Journal of Environmental and Occupational Medicine, 2002, 19(1): 10-20.

[18]陈文彬, 王有赤. 诊断学. 北京: 人民卫生出版社, 2002: 404-425.

Chen W B, Wang Y C. Diagnostics. Beijing: People’s Medical Publishing House, 2002: 404-425. (in Chinese)

[19]Sura P, Ristic N, Bronowicka P, Wrobel M. Cadmium toxicity related to cysteine metabolism and glutathione levels in frog Rana ridibunda tissues. Comparative Biochemistry and Physiology, 2006, C142: 128-135.

[20]Chan P K, Cheng S H. Cadmium-induced ectopic apoptosis in zebrafish embryos. Archive Toxicology, 2003, 77: 69-79.

[21]Isani G, Andreani G, Cocchioni F, Fedeli D, Carpene E, Falcioni G. Cadmium accumulation and biochemical responses in sparus aurata following sub-lethal cadmium exposure. Ecotoxicology Environmental Safety, 2009, 72: 224-230.

[22]汪纪仑, 张英, 裔传卉, 邹辉, 刘宗平. 镉对大鼠原代肝细胞的毒性损伤. 中国兽医学报, 2010, 30(4): 527-530.

Wang L J, Zhang Y, Yi C H, Zou H, Liu Z P. Cytoxicity induced by cadmium in primary cultured rat hepatocytes. Chinese Journal of Veterinary Science, 2010, 30(4): 527-530. (in Chinese)

[23]Klaassen C D. Casarett and Doull’S Toxicology: the Basic Science of Poisons. 6th edition. New York: McGraw-Hill, 2001: 716-720.

[24]卞建春, 郭恒杰, 王捍东, 刘学忠, 袁燕, 任建新, 刘宗平, 王宗元, 康世良, 武瑞. 镉暴露家兔睾丸和肝脏中小分子金属结合蛋白的分离与比较. 中国农业科学, 2009, 42(3): 1091-1099.

Bian J C, Guo H J, Wang H D, Liu X Z, Yuan Y, Ren J X, Liu Z P, Wang Z Y, Kang S L, Wu R. Isolation and comparison of low-molecular-weight metal binding protein in testis and liver of rabbits treated with cadmium. Scientia Agricultura Sinica, 2009, 42(3): 1091-1099. (in Chinese)

[25]金慧英, 胡惠民, 周雍, 李法卿, 谭维国, 李素芹, 陈华标. 急性镉中毒的肝脏损伤机制及金属硫蛋白的保护作用. 中华劳动卫生职业病杂志, 1998, 16(1): 43-46.

Jin H Y, Hu H M, Zhou Y, Li F Q, Tan W G, Li S Q, Chen H B. Mechanism of liver injury in cadmium toxication and protection of metallothionein. Chinese Journal of Industrial Hygiene and Occupational Diseases, 1998, 16(1): 43-46. (in Chinese)

[26]Nordberg G F. Historical perspectives on cadmium toxicology. Toxicology and Applied Pharmacology, 2009, 238: 192-200.

Related Articles 15

[1]	SHU JingTing,JI GaiGe,SHAN YanJu,ZHANG Ming,JU XiaoJun,LIU YiFan,TU YunJie,SHENG ZhongWei,TANG YanFei,JIANG HuaLian,ZOU JianMin. Expression Analysis of IGF1-PI3K-Akt-Dependent Pathway Genes in Skeletal Muscle and Liver Tissue of Yellow Feather Broilers [J]. Scientia Agricultura Sinica, 2021, 54(9): 2027-2038.
[2]	ZHOU Liang,XIAO Feng,XIAO Huan,ZHANG YuSheng,AO HeJun. Effects of Lime on Cadmium Accumulation of Double-Season Rice in Paddy Fields with Different Cadmium Pollution Degrees [J]. Scientia Agricultura Sinica, 2021, 54(4): 780-791.
[3]	PENG Ou,LIU YuLing,TIE BaiQing,YE ChangCheng,ZHANG Miao,LI YuanXingLu,ZHOU JunChi,XU Meng,ZHANG Yan,LONG Yong. Effects of Conditioning Agents and Agronomic Measures on Cadmium Uptake by Rice in Polluted Rice Fields [J]. Scientia Agricultura Sinica, 2020, 53(3): 574-584.
[4]	LI ShuaiLan,SHEN Xiao,ZOU ZhengRong. Effect of Petroleum Ether Extract from Solidago canadensis on Liver of Pomacea canaliculata [J]. Scientia Agricultura Sinica, 2019, 52(15): 2624-2635.
[5]	ZHU YaJing, ZHOU YaLi, LIU SuShuang, WEI JiaPing, LIU XiaoLin, SHEN YingZi, ZHAO HaiHong, MA Hao. GmHMADP Involved in Seed Vigor Formation of Soybean Under High Temperature and Humidity Stress and its Study Responsive to Copper and Cadmium Stress [J]. Scientia Agricultura Sinica, 2018, 51(14): 2642-2654.
[6]	Hua YU, YuXian SHANGGUAN, ShiHua TU, YuSheng QIN, Kun CHEN, DaoQuan CHEN, QianCong LIU. Sources of Cadmium Accumulated in Rice Grain [J]. Scientia Agricultura Sinica, 2018, 51(10): 1940-1947.
[7]	WANG Ting, ZHOU Cui, GU YanWen, MA WenChao, LIU Yuan, WEI Hong. Hyperspectral Estimation of Cadmium Content in Tumorous Stem Mustard Based on the Wavelet-Fractal Analysis [J]. Scientia Agricultura Sinica, 2018, 51(1): 71-81.
[8]	CHEN JiangMin, YANG YongJie, HUANG QiNa, HU PeiSong, TANG ShaoQing, WU LiQun, WANG JianLong, SHAO GuoSheng. Effects of Continuous Flooding on Cadmium Absorption and Its Regulation Mechanisms in Rice [J]. Scientia Agricultura Sinica, 2017, 50(17): 3300-3310.
[9]	XU Chuang, ZHU Kui-ling, CHEN Yuan-yuan, YANG Wei, XIA Cheng, ZHANG Hong-you, WU Ling, SHU Shi, SHEN Tai-yu, YU Hong-jiang, XU Qiu-shi, ZHANG Zi-yang . Isolation Identification and Bioinformatics of Differences Protein in Plasma of Cows Suffer from Fatty Liver with SELDI-TOF-MS Techniques [J]. Scientia Agricultura Sinica, 2016, 49(8): 1585-1598.
[10]	WANG Xue-hua, DAI Li. Immobilization Effect and Its Physiology and Biochemical Mechanism of the Cadmium in Crop Roots [J]. Scientia Agricultura Sinica, 2016, 49(22): 4323-4341.
[11]	LU Mei-bin, CHEN Zhi-jun, LI Wei-xi, HU Xue-xu, LI Jing-mei, WANG Bu-jun. Survey and Dietary Exposure Assessment of Cadmium in Wheat from Two Main Wheat-Producing Regions in China [J]. Scientia Agricultura Sinica, 2015, 48(19): 3866-3876.
[12]	WANG Lin-feng, JIA Shao-dan, YANG Gai-qing, ZHU He-shui, LIU Ru-yi, YAN Ping, LI Ming, YANG Guo-yu. Study on the Effect of Lipopolysaccharide on Hepatic Metabolism in Dairy Goat Liver [J]. Scientia Agricultura Sinica, 2015, 48(18): 3701-3710.
[13]	ZHU Zhi-wei, CHEN Ming-xue, MOU Ren-xiang, CAO Zhao-yun, ZHANG Wei-xing, LIN Xiao-yan. Advances in Research of Cadmium Metabolism and Control in Rice Plants [J]. Scientia Agricultura Sinica, 2014, 47(18): 3633-3640.
[14]	SONG Wen-en, CHEN Shi-bao. The Toxicity Thresholds (ECx) of Cadmium (Cd) to Rice Cultivars as Determined by Root-Elongation Tests in Soils and Its Predicted Models [J]. Scientia Agricultura Sinica, 2014, 47(17): 3434-3443.
[15]	SUN Cong, CHEN Shi-Bao, SONG Wen-恩, LI Ning. Accumulation Characteristics of Cadmium by Rice Cultivars in Soils and Its Species Sensitivity Distribution [J]. Scientia Agricultura Sinica, 2014, 47(12): 2384-2394.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effect of Sub-chronic Cadmium Exposure on Liver Biochemical Index in Wuzhishan Pig

PDF

Cited