Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (1): 77-84.doi: 10.3864/j.issn.0578-1752.2012.01.009

• PLANT PROTECTION • Previous Articles     Next Articles

Insecticidal Activities and Active Ingredients of Pronephrium megacuspe

 HUANG  Su-Qing, XU  Han-Hong, TIAN  Yong-Qing, WEI  Xiao-Yi   

  1. 1.华南农业大学天然农药与化学生物学教育部重点实验室，广州 510642
    2.中国科学院华南植物园，广州510650
  • Received:2011-01-24 Online:2012-01-01 Published:2011-10-08

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Abstract: 【Objective】 The objective of this study is to research the active ingredients of Pronephrium megacuspe.【Method】Insecticidal activities and action mode were determined by bioassay. The active ingredients were isolated from the extract by activity-guided fractionation with column chromatography, thin layer chromatography (TLC) and recrystallization, and the structures were identified predominantly on the basis of MS and NMR data.【Result】The results showed that the methanol extract of P. megacuspe possessed toxicity against the adult of Musca domestica, the 4th instar larva of Aedes albopictus and Myzus persicae, and had antifeedant activity to the 3rd instar lavae of Plutella xylostella, Ostrinia furnacalis and Pieris rapae. 7-hydroxy-5-methoxy -6,8-dimethyl-flavanone, 2′,4′-dihydroxy-3′-methyl-6′-methoxychalcone, 5,7-dihydroxy-6,8-dimethylflavanone and 5-hydroxy-6,7- dimethoxyflavone were isolated and identified from the methanol extract of P. megacuspe. The results of bioassay showed that all compounds possessed antifeedant activity to the 3rd instar lavae of O. furnacalis with the average antifeedant rate above 50% at a concentration of 50 mg•L-1 at 24 h after treatment. Four compounds exhibited no contact activity against the 3rd instar lavae of O. furnacalis at a dosage of 2 ?g/pest. 5,7-dihydroxy-6,8-dimethylflavanone possessed weak antifeedant activity against the 3rd instar lavae of P. xylostella with an average antifeedant rate of 40.51% at 24 h after treatment, and the mortality was 33.76% at a dosage of 1 ?g/pest at 48 h after treatment. The other three compounds had no contact toxicity to the 3rd instar lavae of P. xylostella. 【Conclusion】P. megacuspe has insecticidal activities against various insect pests. Four compounds were isolated and identified from the methanol extract of P. megacuspe. They have the similar mother structure, but different on the number of replaced methylium and its place, and have different activities. Therefore the other compounds and the structure-activity relationship of P. megacuspe need to be further investigated as a biopesticide.

Key words: Pronephriummegacuspe, insecticidalactivity, activitycompound, toxicity

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