薏米多酚细胞抗氧化及HepG2细胞毒性和抗增殖作用

doi:10.3864/j.issn.0578-1752.2013.14.015

Abstract

Abstract: 【Objective】The objective of this study is to learn and analyze the antioxidant capacity of adlay extracts, especially that of the bound part , and to determine the contribution of the bound part to the total antioxidant capacity. The toxicity and anti-proliferative effect of adlay polyphenols on liver cancer cells were also studied. In the meanwhile, the functions of adlay extracts were evaluated and analyzed at cell level. 【Method】 Guizhou heigu adlay, Liaoning 5 adlay and Longyi 1 adlay were used as raw materials, the intracellular ORAC was evaluated by building cell models of free or bound polyphenols of adlay extracts. With human hepatoma cells HepG2 as the research object, CC50 for cytotoxicity and EC50 for anti-proliferative effect were determined and analyzed.【Result】The antioxidant capacity of bound polyphenol is significantly higher than that of the free part. The content of totel polyphenol and the antioxidant capacity are obviously different among different species. Liaoning 5 adlay and Longyi 1 adlay are significantly better than Guizhou Heigu adlay. The adlay extracts have an obvious effect on inhibition of proliferation of human liver cancer cells, and substantially in the experimental concentration range, the adlay sample itself has no cytotoxicity.【Conclusion】As a traditional Chinese medical and edible whole grain foods, adlay has abundant polyphenols and strong antioxidant capacity. The antioxidant value significantly improved after considering the existence of bound polyphenols. Besides, polyphenol extracts of adlay have an obvious anti-proliferative effects on tumor cells.

Key words: adlay , polyphenols , CAA , cytotoxicity , anti-proliferation

WANG Li-Feng-1, CHEN Jing-Yi-1, XIE Hui-Hui-1, WANG Yu-Mei-1, SHI Jia-Yi-1, LIANG Zheng-Chang-2, LIU Rui-Hai-2, JU Xing-Rong-1, YUAN Jian-1. Cytotoxicity and Anti-Proliferation Effect on HepG2 Cells and Cellular Antioxidant Activity(CAA) of Adlay Polyphenols[J].Scientia Agricultura Sinica, 2013, 46(14): 2990-3002.

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