利用酵母双杂交系统筛选感病番茄中蔬四号与无毒蛋白AvrPto、AvrPtoB互作的蛋白

doi:10.3864/j.issn.0578-1752.2011.23.022

Abstract

Abstract: 【Objective】 The objective of this study is to research the function of AvrPto and AvrPtoB as virulence factor in the susceptible tomato by yeast two-hybrid system. 【Method】 The total RNA of Zhongshusihao was extracted, and dscDNA was amplified by long distance PCR. pGBKT7-AvrPto and pGBKT7-AvrPtoB were constructed. The AvrPto and AvrPtoB interactive proteins were screened by two-hybrid system. 【Result】 In the susceptible tomato cultivar Zhongshusihao cDNA library, 7 proteins interacting with AvrPto were identified, 2 proteins interacting with AvrPtoB were identified. Ribulose bisphosphate carboxylase small chain which can interact with both AvrPto and AvrPtoB showed that AvrPto and AvrPtoB may participate in destroying host photosynthesis. Other proteins such as chloroplastic protoporphyrinogen IX oxidase, chloroplast aldolase which participate in host photosynthesis, and chloroplast elongation factor TufA, transcription elongation factor were also participated in protein synthesis. 【Conclusion】According to the results, it was presumed that AvrPto, AvrPtoB interacting with photosynthesis proteins affected Calvin cycle, the synthesis of chlorophyl related protein. By this way, mesophyll cell of host invation site died.

Key words: Pseudomonas syringae pv. tomato, avirulence gene, yeast two-hybrid system

ZHANG Yue-Juan, ZHAO Ting-Chang, YANG Yu-Wen. Screening of the Interactive Proteins of Avirulent Proteins AvrPto or AvrPtoB in Susceptible Tomato Cultivar Zhongshusihao by Yeast Two-Hybrid System [J].Scientia Agricultura Sinica, 2011, 44(23): 4939-4944.

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Screening of the Interactive Proteins of Avirulent Proteins AvrPto or AvrPtoB in Susceptible Tomato Cultivar Zhongshusihao by Yeast Two-Hybrid System

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