Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4593-4599.doi: 10.3864/j.issn.0578-1752.2011.22.005

• PLANT PROTECTION • Previous Articles     Next Articles

Development of a SSR Molecular Marker for Puccinia graminis f. sp. tritici

 WANG  Xi, LIU  Tai-Guo, XIANG  Wen-Sheng, CHEN  Wan-Quan   

  1. 1.中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100193
    2.东北农业大学生命科学学院，哈尔滨 150030
  • Received:2011-03-04 Online:2011-11-15 Published:2011-05-20

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Abstract: 【Objective】The objective of this study is to build a simple, quick and accurate molecular technique to detect Puccinia graminis f. sp. tritici and forecast this destructive disease. 【Method】A microsatellite-enriched genomic library of Pgt was constructed by using the method of FIASCO. Based on the sequence of Pgt microsatellite, pairs of specific SSR primers were developed and screened. 【Result】The primer Pgtfssr1 (f/r) generated a polymorphic pattern displaying a 395 bp DNA fragment specific for Pgt, whereas no DNA fragment was obtained in other 24 non-target wheat fungal pathogens. The existence of specific DNA fragment was detected in the infected wheat tissues at 30 h post-inoculation, and the sensitivity of this molecular marker was Pgt DNA template of 1 ng•μL-1.【Conclusion】A specific SSR marker for the detection of wheat stem rust has been successfully developed, which could be used for the early diagnosis and forecast of wheat stem rust.

Key words: Puccinia graminis f. sp. tritici, microsatellite marker, FIASCO, molecular detection

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