piggyBac转座子介导的转基因家蚕丝腺生物反应器研究进展

doi:10.3864/j.issn.0578-1752.2011.21.018

Abstract

Abstract: This paper reviewed three key steps of recombinant protein expression by silk gland bioreactor of piggyBac- mediated transgenic silkworm. Firstly, the efficient insertion of foreign gene into the genome. Secondly, high-efficiency detection and identification of the transgenic silkworm. Finally, the expression and purification of the active recombinant protein. Meanwhile,it also introduced the advances in research of this kind of bioreactor. The review provided effective information for the future researches.

Key words: silkworm, piggyBac, transgene, silk gland bioreactor

ZHONG Bo-Xiong, WEI Hao, ZHUANG Lan-Fang. Advances in Research of Silk Gland Bioreactor of piggyback –Mediated Transgenic Silkworm[J].Scientia Agricultura Sinica, 2011, 44(21): 4488-4498.

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