Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (16): 3463-3468.doi: 10.3864/j.issn.0578-1752.2011.16.020

• VETERINARY SCIENCE • Previous Articles     Next Articles

Effects of Microbial Fermentation of Corn Straw Mattress Material on Immunological Function of Mice

GAO  Jin-Bo, NIU  Zhong-Xiang   

  1. 1. 山东农业大学动物医学院
    2. 山东农业大学动物科技学院/动物生物技术与疫病防治重点实验室
  • Received:2010-10-11 Revised:2011-03-17 Online:2011-08-15 Published:2011-04-14
  • Contact: Jin-Bo GAO E-mail:jinbogao1985@163.com

Abstract: 【Objective】 The aim of this experiment was to study the effect of microbes in fermented corn stalk on immunologic function of mice.【Method】 Forty mice at 4-week-old of age were divided into four groups randomly and raised to establish different kinds of fermention corn stalk with microbes group as well as control group (without addition of microbes). In the experiments, the antibody titer, contents of IL-4 and IFN-γ in serum, the total number of white blood cell (WBC), lymphocyte (LYM) and red blood cell (RBC), and mean corpuscular haemoglobin concentration (MCHC) in blood were determined. The count of Lactobacillus in cecal content and the anti-fatigue time of swimming of mice were examined.【Result】The results showed that microbes in fermented corn stalk significantly promoted the level of antibody and the count of Lactobacillus in cecal content, RBC and MCHC in blood (P<0.05). The WBC and LYM in blood, the content of IFN-γ, IL-4 were also increased (P>0.05). The result of anti-fatigue experiment showed that the microbes in fermented corn stalk significantly increased the time of swimming of mice (P<0.05). 【Conclusion】 Microbes in fermented corn stalk can significantly improve the immune function of mice and other indicators.

Key words: fermention corn stalk, microbes, mice, immunologic function, antibody, cytokines

[1]Tiquia S M, Tam N F Y, Hodgkiss I J. Microbial activities during composting of spent pig-manure sawdust litter at different moisture contents. Bioresource Technology, 1996, 55: 201-206.

[2]杨朝飞. 加强禽畜粪便污染防治迫在眉睫. 生态与自然保护, 2001, 2: 33-35.

Yang C F. Pollution control of livestock breeding wastes is extremely urgent. Ecology and Natural Conservation, 2001, 2: 33-35. (in Chinese)

[3]Tiquia S M, Tam N F Y, Hodgkiss I J. Effects of bacterial inoculum and misture adjustment on composting of pig manure. Environmental Pollution, 1997, 96(2): 161-171.

[4]李荣林, 杨胜林, 何  勇. 发酵床养猪及常规养猪的对比分析. 贵州农业科学, 2009, 37(11): 122-124. 

Li R L, Yang S L, He Y. Comparative analysis of pig raising between fermenting bed and conventional method. Guizhou Agricultural Sciences, 2009, 37(11): 122-124. (in Chinese)

[5]王远孝, 王 恬. 发酵床养猪技术的应用研究进展. 动物医学进展, 2010, 31: 193-196.

Wang Y X, Wang T. Progress on and application of biology bed in pigs raising. Progress in Veterinary Medicine, 2010, 31: 193-196. (in Chinese)

[6]Tam N F Y, Vrijmoed L L P. Effects of commercial bacterial products on nutrient transformations of pig manure in a pig-on-littersystem. Water Management and Research, 1990, 8(5): 363-373.

[7]Chan D K O, Chaw D, Lo C Y Y. Management of the sawdust litter in the ‘pig-on-litter’ system of pig waste treatment. Resources, Conservation and Recycling, 1994(11): 51-72.

[8]Morrison R S, Hemsworth P H, Cronin G M, Campbell R G. The social and feeding behaviour of growing pigs in deep-litter, group housing systems. Applied Animal Behaviour Science, 2003(82): 173-188.

[9]Karlen G A M, Hemsworth P H, Gonyou H W, Fabrega E, Strom A D, Smits R J. The welfare of gestating sows in conventional stalls and large groups on deep litter. Applied Animal Behaviour Science, 2007, 105(3): 87-101.

[10]朱  洪, 常志州, 叶小梅, 费辉盈. 基于畜禽废弃物管理的发酵床技术研究:Ⅲ高湿热季节养殖效果评价. 农业环境科学学报, 2008, 27(1): 354-358.

Zhu H, Chang Z Z, Ye X M, Fei H Y. Study on deep litter system for management of livestock manure evaluation on effects of deep litter system on pig growth in high temperature and RH season. Journal of Agro-Environment Science, 2008, 27(1): 354-358. (in Chinese)

[11]武华玉, 乔  木, 郭万正, 彭先文, 宋忠旭, 郭  锐, 梅书棋. 生物发酵床养猪效果研究. 湖北农业科学, 2009, 48(12): 3090-3094.

Wu H Y, Qiao M, Guo W Z, Peng X W, Song Z X, Guo R, Mei S Q. Study on the effect of raising pig on biological fermentation bed. Hubei Agricultural Sciences, 2009, 48(12): 3090-3094. (in Chinese)

[12]何明清, 程安春. 动物微生态学. 成都: 四川科技出版社, 2004.

He M Q, Cheng A C. Animal Microecology. Chengdu: Sichuan Science and Technology Press, 2004. (in Chinese)

[13]龙碧波. 海南野生绞股蓝抗运动性疲劳的实验研究. 现代预防医学, 2009, 36(4): 712-716.

Long B B. Experimental research on the anti-fatigue effect of extraction of gynostemma pen taphyllum. Modern Preventive Medicine, 2009, 36(4): 712-716. (in Chinese)

[14]Reiner S L, Seder R A. T helper cell differentiation in immune response. Current Opinion in Immunology, 1995, 7(3): 360-366.

[15]Guo L Y, Jane H L, William E. Probabilistic regulation of IL-4 production in Th2 cells: accessibility at the IL-4 locus. Immunity, 2004, 20: 193-203.

[16]Sun P, Wang J Q, Zhang H T. Effects of bacillus subtilis natto on performance and immune function of preweaning calves. Journal of Dairy Science, 2010, 12(93): 5851-5855.

[17]Sakka K, Kimura T, Karita S, Ohmiya K. Molecular breeding of cellulolytic microbes. Journal of Bioscience and Bioengineering, 2000, 90(3): 227-233.

[18]Guerra N P, Bernárdez P F, Castro L P. Production of four potentially probiotic lactic acid bacteria and their evaluation as feed additives for weaned piglets. Animal Feed Science and Technology, 2007(134): 89-107.

[19]Scharek L, Altherr B J, Tölke C. Influence of the probiotic Bacillus cereus var. Toyoi on the intestinal immunity of piglets. Veterinary Immunology and Immunopathology, 2007, 120(3-4): 136-147.

[20]Suzuki D, Furukawa K, Kimura F, Shimizu H, Yoshidome H, Ohtsuka M, Kato A, Yoshitomi H, Miyazaki M. Effects of perioperative immunonutrition on cell-mediated immunity, T helper type 1(Th1)/ Th2 differentiation and Th17 response after pancreaticoduodenectomy. Surgery, 2010, 148(3): 573-581.

[21]Dlugovitzky D, Fiorenza G, Farroni M, Bogue C, Stanford C, Stanford J. Immunological consequences of three doses of heat-killed mycobacterium vaccae in the immunotherapy of tuberculosis. Respiratory Medicine, 2006, 100(6): 1079-1087.

[22]Christos D. Nonsymbiotic hemoglobins and stress tolerance in plants. Plant Science, 2009, 176(4): 433-440.

[23]Siegel I, Liu T L, Gleicher V. The red cell immune system. The  Lancet, 1981(2): 556-559.

[24]Watanabe T, Takeda T, Omiya S, Hikoso S, Yamaguchi O, Nakano Y, Higuchi Y, Nakai A, Abe Y, Aki-Jin Y, Taniike M, Mizote I, Matsumura Y, Shimizu T, Nishida K, Imai K, Hori M, Shirasawa    T, Otsu K. Reduction in hemoglobin-oxygen affinity results in     the improvement of exercise capacity in mice with chronic heart failure. Journal of the American College of Cardiology, 2008, 52(9): 779-786.

[25]Hoy J A, Hargrove M S. The structure and function of plant hemoglobins. Plant Physiology and Biochemistry, 2008, 46(3): 371-379.

[26]何明清, 程安春. 动物微生态学. 成都: 四川科技出版社, 2004.

He M Q, Cheng A C. Animal Microecology. Chengdu: Sichuan Science and Technology Press, 2004. (in Chinese)

[27]Seegers F M L. Lactobacilli as live vaccine delivery vectors:progress and prospects. Trends Biotechnology, 2002, 20(12): 508-515.
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