FLP/FRT位点特异性重组系统在高等真核生物中的研究进展

doi:10.3864/j.issn.0578-1752.2011.15.021

Abstract

Abstract: FLP/FRT site-specific recombination system derived from 2 μm plasmid of yeast has been widely used in Arabidopsis thaliana, Oryza sativa Linnaeus, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans and other higher eukaryotic organisms, and gradually become one of the powerful tools of genetic manipulation in transgenic animals and plants research areas. This review introduced the recombination principles of FLP/FRT system and its application in higher eukaryotes, and systematically summarized the main achievements of the system in transgenic plants, mammals, insects and other higher eukaryotic model organisms. In addition, the main problem, application prospect and developmental trend of the FLP/FRT system were discussed in this review.

Key words: FLP/FRT system, site-specific recombination, transgene, higher eukaryotes

ZHAO Ai-Chun, LONG Ding-Pei, TAN Bing, XU Long-Xia, XIANG Zhong-Huai. Progress in Research of FLP/FRT Site-Specific Recombination System in Higher Eukaryotes[J].Scientia Agricultura Sinica, 2011, 44(15): 3252-3263.

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Progress in Research of FLP/FRT Site-Specific Recombination System in Higher Eukaryotes

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