高压液相色谱-二极管阵列,酚酸,鉴定 ," /> 高压液相色谱-二极管阵列,酚酸,鉴定 ,"/> HPLC-PAD,phenolic acids,identification,"/> <font face="Verdana">Identification and Determination of Phenolic Acids in Purple Yam#br# </font>

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (12): 2527-2532 .doi: 10.3864/j.issn.0578-1752.2010.12.017

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Identification and Determination of Phenolic Acids in Purple Yam#br#

YU Dong, FANG Zhong-xiang, YANG Hai-hua, ZHANG Jin-jie, YE Xing-qian, WU Dan#br#   

  1. (浙江大学食品科学与营养系)
  • Received:2010-01-12 Revised:2010-04-05 Online:2010-06-15 Published:2010-06-15
  • Contact: YE Xing-qian

Abstract:

【Objective】 As a kind of multipurpose bioactive compound, phenolic acids are widely existed in the plant kingdom. Phenolic acids from purple yam (from Huangyan district, Taizhou city, Zhejiang province) were identified in order to provide references for subsequent analysis on other natural products. 【Method】 Phenolic acids in the purple yam were identified and determined by the method of high performance liquid chromatography (HPLC), photodiode array detection (PAD). 【Result】 The phenolic acids were mainly present in soluble form. Sinapic acid and ferulic acid dominated in both soluble and insolulble fraction and the content of sinapic acid was higher than that of ferulic acid. The total content of soluble phenolic acid was 356.71 μg&#8226;g-1 (dry weight) and that of insoluble phenolic acid was 26.92 μg&#8226;g-1 (dry weight). 【Conclusion】 HPLC-PAD-MS is a fast and effective method to analyze phenolic acids in purple yam.

Key words: HPLC-PAD')">HPLC-PAD, phenolic acids, identification

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