Abstract:

【Objective】 The purpose of this experiment is to search all genes related to potassium absorption and transportation in major crops (except rice), and analyze their phylogenetic relationships. 【Method】 With the help of comparative genome analysis method, searching was conducted across GenBank, EMBL, DDBJ, PDB NR database. Phylogenetic analysis was implemented within the searching result. 【Result】 44 genes with complete cds related to potassium transporter families have been identified in wheat, barley, maze, tobacco etc (except rice). Among these genes, 8 from barley, maze, grape and broad bean fall into HAK/KUP/KT family, 27 from wheat, barley, maze, potato, tomato, tobacco etc into AKT family, 4 from wheat and barley into HKT family and 4 from barley, tobacco and potato into KCO family. KEA family is represented by only one gene from maize. In this research, ZM_BFb0024K15, ZM_BFc0028P03 and ZM_BFb0207I17 cloned in maize genome sequencing project were firstly identified as potassium transporters. The first two genes belong to HAK/KUP/KT family and the third to KEA family. Phylogenetic analysis showed that genes from each family are closely related to the query gene. The comparative identities are from 33% to 84%. 【Conclusion】 There are many genes related to potassium absorption and transportation in major crops (except rice), and many of them are closely related to arabidopsis potassium genes.

Key words: crops')">crops, potassium transporters, phylogenetic analysis, comparative genome analysis