中国农业科学 ›› 2009, Vol. 42 ›› Issue (1): 209-209 .

• 园艺 • 上一篇    下一篇

轮套作对黄瓜土壤微生物多样性及产量的影响

吴凤芝   

  1. 东北农业大学园艺学院
  • 收稿日期:2008-01-20 修回日期:2008-05-13 出版日期:2009-01-10 发布日期:2009-01-10
  • 通讯作者: 吴凤芝

Effect of Rotation and Interplanting on Cucumber Yield and Soil Microbial Diversity

  1. 东北农业大学园艺学院
  • Received:2008-01-20 Revised:2008-05-13 Online:2009-01-10 Published:2009-01-10
  • Contact: WU Feng-zhi

摘要:

【目的】研究轮套作对黄瓜各个时期的土壤微生物多样性及黄瓜产量的影响。【方法】采用随机扩增多态性DNA分析(random amplified polymorphism DNA,RAPD)方法。【结果】轮作处理中,小麦和大豆轮作处理可以显著提高黄瓜土壤微生物群落DNA序列多样性各指数(P<0.05),其黄瓜产量极显著高于对照(P<0.01);与对照相比,毛葱套作处理显著提高黄瓜土壤微生物群落DNA序列多样性各指数,其黄瓜产量极显著高于对照(P<0.01)。【结论】小麦、大豆轮作和毛葱套作有利于改善土壤微生态环境,提高黄瓜产量。

关键词: 黄瓜, 轮套作, 土壤微生物群落, DNA序列多样性, 产量, RAPD

Abstract:

【Objective】 The objective of the experiment is to study the effect of rotation and interplanting on microbial community diversity in different growth periods and cucumber yield. 【Method】 RAPD techniques were adopted as research methods. 【Result】 Wheat-soybean rotation significantly improved the cucumber microbial DNA sequence diversity index (P<0.05), and significantly increased yield of cucumber (P<0.01).Compared to control, onion interplanting treatment also significantly increased cucumber microbial DNA sequence diversity index and yield of cucumber (P<0.01).【Conclusion】 Wheat-soybean rotation and garlic interplanting could facilitate improving soil micro-ecological environment and increase the yield of cucumber.

Key words: cucumber, rotation-intercropping, soil microbial community, DNA sequence diversity, yield, RAPD