中国农业科学 ›› 2007, Vol. 40 ›› Issue (7): 1543-1549 .

• 研究简报 • 上一篇    下一篇

甘蓝型油菜3种类型雄性不育系花药败育的细胞学研究

聂明建,王国槐,朱卫平   

  1. 湖南农业大学农学院
  • 收稿日期:2006-02-27 修回日期:2006-06-23 出版日期:2007-07-10 发布日期:2007-07-10

Cytology Research on the Anther Abortion of Three Male Sterility Lines in Rapeseed(Brassica napus L.)

  

  1. 湖南农业大学农学院
  • Received:2006-02-27 Revised:2006-06-23 Online:2007-07-10 Published:2007-07-10

摘要: 【目的】了解甘蓝型油菜不同类型雄性不育系的败育时期与细胞学特征。【方法】以细胞质雄性不育系392A、隐性核不育系86A与显性核不育系629A为材料,保持系392B作对照,采用常规石蜡切片技术,对甘蓝型油菜雄性不育系的花药败育过程进行细胞学研究。【结果】86A花粉母细胞不能进行减数分裂;629A花粉母细胞进入减数第一分裂后解体、不能完成减数分裂;而392A是否败育,则受温度的影响,在高温下(日均温≥10℃)不产生花粉囊,花药败育,低温条件下则发育正常,雄蕊可育。【结论】86A和 629A同属花粉母细胞败育型,392A属无花粉囊败育型,392A的温度敏感时期是在孢原细胞期前。392A、86A和629A的花药败育与绒毡层发育异常有密切的关系。

关键词: 甘蓝型油菜, 雄性不育系, 花药败育, 细胞解剖学

Abstract: With cytoplasmic male sterility line 392A, recessive double-family genic male sterility line 86A and dominant double-family genic male sterility line 629A in rapeseed(Brassica Napus L) as materials, we do cell anatomy research on the anther abortion processes of male sterility lines in rapeseed(Brassica Napus L) and make a comparison with 392B. The result indicates that 86A and 629A both belong to pollen female cell abortion and whether 392A will have an abortion is decided by temperature. When it is≥10℃ on the average during the day, there will be no pollen sacs and sterility arises. When the temperature is lower, fertility happens. We also find that 392A is influenced by temperature before the period of archesporical cells.

Key words: rapeseed(Brassica Napus L), male sterility line, anther abortion, cell anatomy