中国农业科学 ›› 2019, Vol. 52 ›› Issue (21): 3733-3747.doi: 10.3864/j.issn.0578-1752.2019.21.002
叶桑,崔翠,郜欢欢,雷维,王刘艳,王瑞莉,陈柳依,曲存民,唐章林,李加纳,周清元()
收稿日期:
2019-05-28
接受日期:
2019-07-11
出版日期:
2019-11-01
发布日期:
2019-11-12
通讯作者:
周清元
作者简介:
叶桑,Tel:13068300612;E-mail: 基金资助:
YE Sang,CUI Cui,GAO HuanHuan,LEI Wei,WANG LiuYan,WANG RuiLi,CHEN LiuYi,QU CunMin,TANG ZhangLin,LI JiaNa,ZHOU QingYuan()
Received:
2019-05-28
Accepted:
2019-07-11
Online:
2019-11-01
Published:
2019-11-12
Contact:
QingYuan ZHOU
摘要:
目的 菜籽油在烹饪、食品加工及工业生产中广泛应用,因此,根据生产需要改善菜籽油脂肪酸组分是油菜育种的重要目标。通过对2种环境下甘蓝型油菜主要脂肪酸组成进行QTL定位分析,寻找甘蓝型油菜脂肪酸组分的QTL及影响本群体脂肪酸组分的候选基因。方法以人工合成甘蓝型油菜10D130和甘蓝型油菜常规品种中双11构建高世代重组自交系(RIL)为研究材料,分别于2016-2017年和2017-2018年2个年度在重庆市北碚区2个不同的环境中设置田间试验,收获自交种子,采用气相色谱法3次重复对种子的脂肪酸组分进行分析。利用油菜6K SNP芯片对该RIL群体进行基因分型,DNA样品预处理及芯片处理严格按照Illumina Inc 公司Infinium HD Assay Ultra操作说明进行。取最小阈值LOD 2.0利用JoinMap4.0软件构建高密度遗传连锁图谱。通过QTL IciMapping V4.1完备区间作图法对油菜主要脂肪酸组成进行QTL定位。结果 2种环境中,两亲本各性状间差异及RIL群体各性状在株系间差异均达到显著或极显著水平,且6种脂肪酸含量在2个环境中均表现为连续分布,适合进行QTL检测。构建用于QTL定位的遗传图谱包含1 897个多态性SNP标记,覆盖甘蓝型油菜基因组3 214.19 cM,平均图距1.69 cM。利用此图谱,在2个环境共检测到位于8条染色体上的23个控制脂肪酸组分QTL位点,与硬脂酸、油酸、亚油酸、亚麻酸、廿碳烯酸和芥酸含量相关的QTL位点分别为6、3、4、5、2和3个,其中在A05、A08和C03染色体上发现多种脂肪酸含量的QTL“富集区”。在A05染色体上检测到亚油酸和亚麻酸含量重叠的主效QTL,亚油酸与亚麻酸表现加性效应相同;在A08和C03上都检测到油酸、廿碳烯酸和芥酸含量重叠的主效QTL,油酸与廿碳烯酸及芥酸表现加性效应相反。与拟南芥脂肪酸代谢基因进行同源性比对分析,在17个QTL置信区间内筛选到22个候选基因,主要通过编码脂肪酸去饱和酶、全羧化酶合酶、碳链延长酶和参与酰基辅酶A生物合成等途径调控脂质的生物合成和代谢。结论 利用甘蓝型油菜6K SNP芯片准确定位了2种环境条件脂肪酸组成的QTL位点,筛选到位于A05、A08和C03染色体上多种脂肪酸QTL的“富集区”,并与拟南芥脂肪酸代谢基因比对出该群体油菜脂肪酸代谢基因,可作为改善油菜籽脂肪酸组成的重要区段及候选基因。
叶桑,崔翠,郜欢欢,雷维,王刘艳,王瑞莉,陈柳依,曲存民,唐章林,李加纳,周清元. 基于SNP遗传图谱对甘蓝型油菜部分脂肪酸 组成性状的QTL定位[J]. 中国农业科学, 2019, 52(21): 3733-3747.
YE Sang,CUI Cui,GAO HuanHuan,LEI Wei,WANG LiuYan,WANG RuiLi,CHEN LiuYi,QU CunMin,TANG ZhangLin,LI JiaNa,ZHOU QingYuan. QTL Identification for Fatty Acid Content in Brassica napus Using the High Density SNP Genetic Map[J]. Scientia Agricultura Sinica, 2019, 52(21): 3733-3747.
"
环境 Environment | 性状 Trait | 亲本 Parents | RIL群体 RIL populations | ||||||
---|---|---|---|---|---|---|---|---|---|
10D130 | ZS11 | 最大值 Max | 最小值 Min | 均值 Average | 标准差 SE | 变异系数 CV (%) | |||
17Cq | C18:0 | 1.21a | 2.84b | 6.17 | 1.00 | 1.77** | 0.61 | 34.47 | |
C18:1 | 18.83A | 64.27B | 83.15 | 13.17 | 38.25** | 19.11 | 49.95 | ||
C18:2 | 10.86A | 23.27B | 31.18 | 6.15 | 14.49** | 4.50 | 31.04 | ||
C18:3 | 7.68a | 9.55b | 12.79 | 4.04 | 8.65* | 1.59 | 18.35 | ||
C20:1 | 8.08A | 0.07B | 19.33 | 0.00 | 10.26** | 5.86 | 57.12 | ||
C22:1 | 53.34A | 0.00B | 57.96 | 0.00 | 26.56** | 17.70 | 66.62 | ||
18Cq | C18:0 | 1.52a | 2.68b | 4.73 | 0.47 | 1.98** | 0.56 | 28.13 | |
C18:1 | 17.91A | 63.03B | 78.79 | 12.69 | 39.14** | 18.03 | 46.07 | ||
C18:2 | 12.08A | 22.94B | 26.41 | 9.27 | 15.47** | 3.94 | 25.46 | ||
C18:3 | 8.92a | 10.44b | 12.20 | 6.14 | 8.89* | 1.36 | 15.27 | ||
C20:1 | 8.86A | 0.91B | 19.99 | 0.70 | 11.20** | 6.43 | 57.44 | ||
C22:1 | 50.71A | 0.00B | 51.62 | 0.00 | 23.02** | 15.79 | 68.59 |
表2
2个环境下主要脂肪酸含量相关分析"
测定指标 Index | C18:0 | C18:1 | C18:2 | C18:3 | C20:1 | C22:1 |
---|---|---|---|---|---|---|
C18:0 | 0.58** | 0.18* | -0.32** | -0.28** | -0.59** | |
C18:1 | 0.65** | 0.36** | -0.13 | -0.68** | -0.95** | |
C18:2 | 0.03 | 0.43** | 0.45** | -0.50** | -0.52** | |
C18:3 | -0.29** | 0.11 | 0.49** | -0.12 | -0.01 | |
C20:1 | -0.31** | -0.77** | -0.57** | -0.28** | 0.56** | |
C22:1 | -0.64** | -0.96** | -0.54** | -0.20** | 0.64** |
表3
油菜主要脂肪酸含量在2个环境中的QTL位点"
性状 Trait | 环境 Envi. | QTL | 染色体 Chr. | 标记区间 Position | 置信区间1) Confidence interval | LOD | 加性效应 Additive effect | 贡献率 R2(%) |
---|---|---|---|---|---|---|---|---|
C18:0 | 17Cq | qA08ST-1 | A08 | S-95505568-S-95506507 | 43.27-48.50 | 6.13 | -0.22 | 14.00 |
qC03ST | C03 | S-177827612-S-95636886 | 216.25-219.73 | 5.11 | -0.20 | 11.59 | ||
18Cq | qA01ST | A01 | S-95637833-S-95664701 | 116.00-118.62 | 4.94 | 0.13 | 5.08 | |
qA05ST | A05 | S-177830309-S-86232724 | 107.59-110.16 | 6.07 | 0.15 | 6.27 | ||
qA08ST-2 | A08 | S-95506569-S-95506120 | 29.90-31.07 | 20.45 | -0.29 | 25.49 | ||
qA09ST | A09 | S-182142581-S-182167880 | 224.50-229.51 | 8.76 | -0.18 | 9.45 | ||
qC03ST | C03 | S-95665809-S-95636886 | 215.70-219.73 | 8.31 | -0.17 | 9.12 | ||
C18:1 | 17Cq | qA05OL | A05 | S-182087654-S-86232724 | 106.33-110.16 | 4.59 | 3.81 | 3.80 |
qA08OL | A08 | S-95507415-S-95663297 | 26.79-30.00 | 31.31 | -11.42 | 34.39 | ||
qC03OL | C03 | S-105305847-S-182158964 | 218.48-222.84 | 29.65 | -10.95 | 31.80 | ||
18Cq | qA05OL | A05 | S-182087654-S-86232724 | 106.33-110.16 | 8.40 | 3.91 | 5.70 | |
qA08OL | A08 | S-95507415-S-95663297 | 26.79-30.00 | 31.83 | -8.66 | 28.28 | ||
qC03OL | C03 | S-105338742-S-182138971 | 219.51-224.50 | 42.44 | -10.75 | 43.68 | ||
C18:2 | 17Cq | qA05LI | A05 | S-182087654-S-86232724 | 106.33-110.16 | 18.43 | -2.37 | 22.67 |
qA08LI | A08 | S-177633794-S-95506569 | 25.61-29.50 | 8.45 | -1.48 | 8.91 | ||
qC03LI-1 | C03 | S-105306222-S-95637726 | 235.80-239.65 | 14.65 | -2.06 | 17.00 | ||
qC03LI-2 | C03 | S-105309588-S-105307276 | 250.03-253.95 | 6.28 | 1.28 | 6.69 | ||
18Cq | qA05LI | A05 | S-182087654-S-86232724 | 106.33-110.16 | 19.71 | -1.84 | 25.25 | |
qA08LI | A08 | S-95507415-S-95663297 | 26.79-30.00 | 9.44 | -1.18 | 10.49 | ||
qC03LI-1 | C03 | S-95637910-S-105307365 | 237.50-239.83 | 11.09 | -1.30 | 12.56 | ||
C18:3 | 17Cq | qA02LN | A02 | S-95666343-S-95638378 | 55.50-64.85 | 4.96 | -0.44 | 8.49 |
qC07LN | C07 | S-105339086-S-179307020 | 6.50-11.97 | 5.84 | 0.51 | 11.66 | ||
18Cq | qA05LN | A05 | S-182087654-S-86232724 | 106.33-110.16 | 33.12 | -1.12 | 23.66 | |
qA09LN | A09 | S-182142581-S-182167880 | 224.50-229.51 | 8.28 | 0.47 | 4.24 | ||
qA10LN | A10 | S-177910390-S-95636447 | 59.94-63.16 | 4.90 | 0.35 | 2.37 | ||
C20:1 | 17Cq | qC03EI | C03 | S-105338742-S-182138971 | 219.51-224.50 | 7.45 | 2.27 | 17.23 |
18Cq | qA08EI | A08 | S-95507415-S-95663297 | 26.79-30.00 | 6.07 | 2.14 | 11.18 | |
qC03EI | C03 | S-105338742-S-182138971 | 219.51-224.50 | 11.44 | 3.05 | 22.69 | ||
C22:1 | 17Cq | qA08ER-1 | A08 | S-95506362-S-95664454 | 10.05-12.58 | 11.30 | 5.00 | 9.39 |
qA08ER-2 | A08 | S-95507415-S-95663297 | 26.79-30.00 | 27.90 | 9.13 | 32.21 | ||
qC03ER | C03 | S-105305847-S-182158964 | 218.48-222.84 | 32.17 | 9.62 | 36.01 | ||
18Cq | qA08ER-2 | A08 | S-95507415-S-95663297 | 26.79-30.00 | 96.68 | 17.53 | 67.26 | |
qC03ER | C03 | S-105305847-S-182158964 | 218.48-222.84 | 50.41 | 8.64 | 16.45 |
表4
在甘蓝型油菜脂肪酸含量QTL置信区间比对拟南芥相关基因获得的候选基因"
染色体 Chr. | 预测基因 Gene prediction | 物理位置 Physical position (Mb) | 拟南芥相关基因 Related genes in A. thaliana | |||
---|---|---|---|---|---|---|
基因名称 Gene name | 基因登录号 Gene accession | E值 E-value | 描述 Description | |||
A01 | BnaA01g27100D | 18.94 | HAL3A | AT3G18030 | E-60 | 辅酶A生物合成过程 Coenzyme A biosynthetic process |
BnaA01g27120D | 18.94 | HAL3B | AT1G48605 | 5E-23 | 辅酶A生物合成过程 Coenzyme A biosynthetic process | |
A02 | BnaA02g13270D | 7.29 | LACS9 | AT1G77590 | 2E-52 | 长链酰基辅酶A合成酶9 Long chain acyl-CoA synthetase 9 |
BnaA02g13310D | 7.31 | KCR1 | AT1G67730 | 2E-74 | β-酮酰辅酶A还原酶1 Beta-ketoacyl-CoA reductase 1 | |
BnaA02g13630D | 7.49 | HCS1 | AT2G25710 | 2E-58 | 全羧化酶合成酶1 Holocarboxylase synthase 1 | |
A05 | BnaA05g26430D | 19.37 | ALIS1 | AT3G12740 | 2E-93 | 参与磷脂转运 Involved in phospholipid transport |
BnaA05g26900D | 19.54 | FAD2 | AT3G12120 | 0 | 不饱和脂肪酸生物合成过程 Unsaturated fatty acid biosynthetic process | |
BnaA05g25110D | 18.65 | ATOBL1 | AT3G14360 | E-156 | 脂质代谢过程 Lipid metabolic process | |
BnaA05g25210D | 18.70 | GLIP3 | AT1G53990 | 4E-17 | 脂质分解过程 Lipid catabolic process | |
BnaA05g25220D | 18.70 | GLIP4 | AT3G14225 | E-117 | 脂质分解过程 Lipid catabolic process | |
BnaA05g26460D | 19.38 | DALL4 | AT1G06800 | 2E-27 | 脂质代谢过程 Lipid metabolic process | |
A08 | BnaA08g09510D | 9.09 | OGOX2 | AT4G20840 | 0 | FAD-结合小檗碱家族蛋白 FAD-binding Berberine family protein |
BnaA08g11130D | 10.19 | FAE1 | AT4G34520 | 0 | 3-酮酰辅酶A合成酶18 3-ketoacyl-CoA synthase 18 | |
BnaA08g11140D | 10.19 | KCS17 | AT4G34510 | 0 | 3-酮酰辅酶A合成酶17 3-ketoacyl-CoA synthase 17 | |
BnaA08g11440D | 10.39 | FAR3 | AT4G33790 | 8E-46 | 脂肪酸还原酶3 FATTY ACID REDUCTASE 3 | |
BnaA08g11650D | 10.51 | MCCB | AT4G34030 | E-119 | 3-甲基巴豆酰辅酶A羧化酶 3-methylcrotonyl-CoA carboxylase | |
BnaA08g12370D | 11.04 | WIN2 | AT4G31750 | 3E-71 | 编码HopW1-1-相互作用蛋白2 Encodes HopW1-1-Interacting protein 2 | |
A09 | BnaA09g39290D | 27.87 | SLD1 | AT3G61580 | 0 | 脂肪酸去饱和酶 Fatty acid desaturase |
A10 | BnaA10g10590D | 9.01 | 未知Unknown | AT5G56350 | 0 | 丙酮酸激酶家族蛋白 Pyruvate kinase family protein |
BnaA10g10730D | 9.08 | END2 | AT5G56480 | 4E-62 | 脂质转移蛋白 Lipid-transfer protein | |
C03 | BnaC03g63920D | 53.41 | OGOX2 | AT4G20840 | 0 | FAD-结合小檗碱家族蛋白 FAD-binding Berberine family protein |
BnaC03g63930D | 53.42 | BBE22 | AT4G20860 | 5E-23 | FAD-结合小檗碱家族蛋白 FAD-binding Berberine family protein | |
BnaC03g64130D | 53.56 | SPHK2 | AT4G21534 | 4E-55 | 二酰基甘油激酶家族蛋白 Diacylglycerol kinase family protein | |
BnaC03g65980D | 55.68 | FAE1 | AT4G34520 | 0 | 3-酮酰辅酶A合成酶18 3-ketoacyl-CoA synthase 18 | |
BnaC03g66040D | 55.81 | KCS17 | AT4G34510 | 0 | 3-酮酰辅酶A合成酶17 3-ketoacyl-CoA synthase 17 | |
BnaC03g66380D | 56.21 | FAR3 | AT4G33790 | 3E-49 | 脂肪酸还原酶3 FATTY ACID REDUCTASE 3 | |
BnaC03g67410D | 57.10 | WIN2 | AT4G31750 | 2E-73 | 编码HopW1-1-相互作用蛋白2 Encodes HopW1-1-Interacting protein 2 |
[1] | 官春云 . 中国油菜产业发展方向. 粮食科技与经济,2011,36(2):5-6. |
GUAN CY. Development direction of rapeseed industry in China. Grain Science and Technology and Economy , 2011,36(2):5-6. (in Chinese) | |
[2] | 沈金雄, 傅廷栋, 涂金星, 马朝芝. 中国油菜生产及遗传改良潜力与油菜生物柴油发展前景.华中农业大学学报 , 2007,26(6):894-899. |
SHEN JX, FU T D,TU J X,MA C Z . Potential in production and genetic improvement of rapeseed and prospect for rape oil-based biodiesel in China.Journal of Huazhong Agricultural University, 2007,26(6):894-899. (in Chinese) | |
[3] | CHANG NW ,HUANG P C. Effects of the ratio of polyunsaturated and monounsaturated fatty acid to saturated fatty acid on rat plasma and liver lipid concentrations. Lipids, 1998,33: 481-487. |
[4] | PETUKHOVI, MALCOBNSON LJ, PRZYBYLSKIR, ARMSTRONG L. Frying performance of genetically modified canola oils. Journal of the American Oil Chemists Society, 1999,76: 627-632. |
[5] | MILLER MR ,BRIDLE A R,NICHOLS P D,CARTER C G . Increased elongase and desaturase gene expression with stearidonic acid enriched diet does not enhance long-chain (n-3) content of seawater Atlantic salmon (Salmo salar L.). The Journal of Nutrition , 2008,138(11):2179-2185. |
[6] | PIAZZA GJ , FOGLIA T A. Rapeseed oil for oleochemical uses. European Journal of Lipid Science and Technology, 2001,103: 405-454. |
[7] | 傅寿仲, 张洁夫 戚存扣,浦惠明,高建芹,陈新军,工业专用型高芥酸油菜新品种选育. 作物学报, 2004,30(5):409-412. |
FU SZ,ZHANG J F,QI C K,PU H M,GAO J Q,CHEN X J . Breeding of high erucic acid rapeseed (B. napus L.) for industrial use.Acta Agronomica Sinica , 2004,30(5):409-412. (in Chinese) | |
[8] | MARTINEZ-RIVAS JM ,SPERLING P,LUHS W,HEINZE.Spatial and temporal regulation of three different microsomal oleate desaturase genes (FAD2) from normal-type and high-oleic varieties of sunflower (Helianthus annuus L.). Molecular Breeding , 2001,8(2):159-168. |
[9] | PIDKOWITCH MS ,GUYEN H T, HEILMANN I, ISCHEBECK T,SHANKLIN . Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil. Proceedings of the National Academy of Sciences of the United States of America, 2007,104(11):4742-4747. |
[10] | SAHAS, ENUGUTTIB, RAJAKUMARIS ,RAJASEKHARAN R.Cytosolic triacylglycerol biosynthetic pathway in oilseeds: molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase.Plant Physiology, 2006,141: 1533-1543. |
[11] |
BAUDS ,LEPINIEC L .Physiological and developmental regulation of seed oil production.Progress in Lipid Research , 2010,49(3):235-249.
doi: 10.1097/WNQ.0b013e3181644e82 pmid: 20102727 |
[12] | HU XY, SULLIVAN-GILBERTM, GUPTAM ,THOMPSON S A. Mapping of the loci controlling oleic and linoleic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.). Theoretical and Applied Genetics, 2006,113: 497-507. |
[13] |
PHAM AT ,SHANNON G J,BILYEU K D. Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil. Theoretical and Applied Genetics , 2012,125(3):503-515.
doi: 10.1007/s00122-012-1849-z |
[14] | YONG HY ,WANGC, BANCROFT I,LIF, WU X, KITASHIBA H,NISHIO T. Identification of a gene controlling variation in the salt tolerance of rapeseed (Brassica napus L.). Planta, 2015,242(1):313-26. |
[15] | FIETCHER RS, HERRMANN D,MULLEN J L, LI Q F, SCHRIDER D R, PRICE N, LIN J J, GROGAN K, KERN A,MCKAY J K. Identification of polymorphisms associated with drought adaptation QTL in Brassica napus by resequencing. G3 Genesgenetics, 2016,6(4):793-803. |
[16] | BEHLAR ,HIRANI A H,ZELMER C D,YU F Q, DILANTHA- FERNANDO W G, MCVETTY P,LI G Y .Identification of common QTL for resistance to Sclerotinia sclerotiorum in three doubled haploid populations of Brassica napus(L.). Euphytica, 2017: 213-260. |
[17] |
HUANG JX , XIONG H X, PAN B, NI X Y, ZHANG X Y,ZHAO J Y. Mapping QTL of flowering time and their genetic relationships with seed weight in Brassica napus. Scientia Agricultura Sinica, 2016,49(16):3073-3083.
doi: 10.3864/j.issn.0578-1752.2016.16.002 |
[18] | YEJ ,YANG Y H,CHEN B, SHI J Q, LUO M Z, ZHAN J P, WANG X F, LIU G H,WANG H Z. An integrated analysis of QTL mapping and RNA sequencing provides further insights and promising candidates for pod number variation in rapeseed (Brassica napus L.). BMC Genomics, , 2017,18(1):18-71. |
[19] | WENJ ,XU J F,LONG Y, WU J G, XU H M, MENG J L,SHI C H . QTL mapping based on the embryo and maternal genetic systems for non-essential amino acids in rapeseed (Brassica napus L.) meal. Journal of the Science of Food and Agriculture , 2016,96(2):465-473 |
[20] | HUANG JX ,CHEN F,ZHANG H Z, NI X Y, WANG Y L, LIU H, YAO X T, XU H M, WANG H, MENG J L, ZHAO J Y. Dissection of additive,epistatic and QTL × environment effects involved in oil content variations in rapeseed.Plant Breeding,2017,136(4):728-737. |
[21] | RABOANATAHIRYN, CHAO HB, DALINH, PUS, YANW, YU LJ, WANG BS ,LI M T. QTL alignment for seed yield and yield related traits in Brassica napus.Frontiers in Plant Science, 2018,9: 1127. |
[22] | BURNS MJ ,BARNES S R,BOWMAN J G, CLARKE M H, WERNER C P,KEARSEY M J. QTL analysis of an intervarietal set of substitution lines in Brassica napus: (i)Seed oil content and fatty acid composition.Heredity ,2003,90(1):39-48. |
[23] | 张洁夫, 戚存扣 浦惠明, 陈松, 陈锋, 高建芹, 陈新军, 顾慧, 傅寿仲 ,甘蓝型油菜主要脂肪酸组成的QTL定位.作物学报, 2008,34(1):54-60. |
ZHANG JF ,QI C K,PU H M, CHEN S, CHEN F, GAO J Q, CHEN X J, GU H,FU S Z .QTL identification for fatty acid content in rapeseed (Brassica napus L.). Acta Agronomica Sinica ,2008,34(1):54-60. (in Chinese) | |
[24] |
ZHAO JY, DIMOV Z,BECKER H C, ECKE W,MOLLERS C . Mapping QTL controlling fatty acid composition in a doubled haploid rapeseed population segregating for oil content. Molecular Breeding ,2008,21(1):115-125.
doi: 10.1007/s11032-007-9113-y |
[25] | LIU LZ, QU CM, WITTKOPB, YIB, XIAOY, HE YJ, SNOWDON RJ ,LI J N. A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L.. PLoS ONE, 2013,8: e83052. |
[26] | SHEN YS ,YANGY,XU E S, GE X H, XIANG Y,LI Z Y. Novel and major QTL for branch angle detected by using DH population from an exotic introgression in rapeseed (Brassica napus L.). Theoretical and Applied Genetics ,2018,131(1):67-78. |
[27] | QU CM ,JIA L D,FU F Y, ZHAO H Y, LU K, WEI L J, XU X F, LIANG Y, LI S M, WANG R,LI J N . Genome-wide association mapping and Identification of candidate genes for fatty acid composition in Brassica napus L . using SNP markers. BMC Genomics,2017,18(1): 232 |
[28] | 王健康 . 数量性状基因的完备区间作图方法 . 作物学报, 2009,35(2):1-7. |
WANG JK. Inclusive composite interval mapping of quantitative trait genes . Acta Agronomica,Sinica 2009,35(2):1-7. (in Chinese) | |
[29] | RUCKERB ,ROBBELEN G. Impact of low linolenic acid content on seed yield of winter oilseed rape (Brassica napus L.). Plant Breed ,1996,115(4):226-230. |
[30] | 刘列钊, 李加纳 ,. 利用甘蓝型油菜高密度SNP遗传图谱定位油酸、亚麻酸及芥酸含量QTL位点. 中国农业科学, 2014,47(1):24-32. |
LIU LZ LI J N. QTL Mapping of oleic acid,linolenic acid and erucic acid content in Brassica napus by using the high density SNP genetic map. Scientia Agricultura Sinica ,2014,47(1):24-32. (in Chinese) | |
[31] | VAN OOIJEN J W. JoinMap******4: Software for the calculation of genetic linkage maps in experimental populations. Netherlands: WagningenUniversity 2006. . |
[32] | KOSAMBI D D. The estimation of map distances from recombination values. Annals ofEugenics , 1944,12: 172-175. |
[33] | LI HH ,YE G Y, WANG J K. A modified algorithm for the improvement of composite interval mapping. Genetics , 2007,175(1):361-374. |
[34] | BOTELLAC, SAUTRON E,BOUDIERE L, MICHAUD M, DUBOTS E, YAMARYO-BOTTE Y, ALBRIEUX C, MARECHAL E, BLOCK M A, JOUHET J. ala10, a phospholipid flippase,controls fad2/fad3 desaturation of phosphatidylcholine in the ER and affects chloroplast lipid composition in Arabidopsis thaliana. Plant Physiology ,2016,170(3):1300-1314. |
[35] |
QIUD ,MORGAN J,SHI J, LONG Y, LIU J, LI R, ZHUANG X, WANG Y, TAN X, DIETRICH E, WEIHMANN T, EVERETT C, VANSTRAELEN S, BECKETT P, FRASER F, TRICK M, BARNES S, WILMER J, SCHMIDT R, LI J, LI D, MENG J,BANCROFTIv. A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theoretical and Applied Genetics , 2006,114(1):67-80.
doi: 10.1007/s00122-006-0411-2 |
[36] | 王欣娜, 阎星颖 李加纳,刘列钊,. 油菜种子油脂分子标记及QTL定位的研究进展. 中国农学通报, 2012,28(24):1-7. |
WANG XN ,YAN X Y, LI J N,LIU L Z. The research progress of lipid molecular markers and QTL mapping in rapeseed. Chinese Agricultural Science Bulletin ,2012,28(24):1-7. (in Chinese) | |
[37] | 张宏军, 肖钢, 谭太龙, 李栒, 官春云, . EMS处理甘蓝型油菜(Brassica napus)获得高油酸材料. 中国农业科学, 2008,41(12):4016-4022. |
ZHANG HJ, XIAO G,TAN T L, LI X,GUAN C Y. High oleate material of rapeseed (Brassica napus) produced by EMS treatment. Scientia Agricultura Sinica ,2008,41(12):4016-4022. (in Chinese) | |
[38] | 陈伟, 范楚川 钦洁, 郭振华, 傅廷栋, 周永明. 分子标记辅助选择改良甘蓝型油菜种子油酸和亚麻酸含量. 分子植物育种, 2011,9(2):190-197. |
CHEN W , FAN C C,QIN J, GUO Z H, FU T D,ZHOU Y M . Genetic improvement of oleic and linolenic acid content through marker- assisted selection in Brassica napus seeds. Molecular Plant Breeding , 2011,9(2):190-197. (in Chinese) | |
[39] | OKULEYJ ,LIGHTNERJ,FELDMANN K, YADAV N, LARK E, BROWSE J .Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. The Plant Cell , 1994,6(1):147-58. |
[40] | LIF , CHEN B,XU K, WU J, SONG W, BANCROFT I, HARPER A L, TRICK M, LIU S,GAO G. Genome-wide association study dissects the genetic architecture of seed weight and seed quality in rapeseed (Brassica napus L.). DNA Research , 2014,21(4):355-367. |
[41] |
WUG , WU Y,XIAO L, LI X, LUC . Zero erucic acid trait of rapeseed (Brassica napus L.) results from a deletion of four base pairs in the fatty acid elongase 1 gene. Theoretical and Applied Genetics ,2008,116(4):491-499.
doi: 10.1007/s00122-007-0685-z |
[42] | WANGN ,WANG YJ,TIAN F, KING G J, ZHANG C Y, LONG Y, SHI L,MENG J L. A functional genomics resource for Brassica napus: Development of an EMS mutagenized population and discovery of FAE1 point mutations by TILLING.New Phytologist , 2008,180(4):751-765. |
[43] | TRESCHS, HEILMANNM, CHRISTIANSENN, LOOSERR, GROSSMANNK. Inhibition of saturated very-long-chain fatty acid biosynthesis by mefluidide andperfluidone,selective inhibitors of 3-ketoacyl-CoA synthases.Phytochemistry, 2012,76 162-171. |
[44] | ROWLANDO,ZHENG H,HEPWORTH S R, LAM P, JETTER R,KUNST L. FAR3 encodes an alcohol-forming fatty acyl-coenzyme A reductase involved in cuticular wS production in Arabidopsis. Plant Physiology , 2006,142(3):866-877. |
[45] | NIKOLAU BJ , OHLROGGE J B, WURTELE E S . Plant biotin- containing carboxylases. Archives of Biochemistry and Biophysics ,2003,414(2):211-222. |
[46] | ZHAO LF ,KATAVIC V,LI F L, HAUGHN G W, KUNST L . Insertional mutant analysis reveals that long-chain acyl-CoA synthetase 1 (LACS1),but not LACS8,functionally overlaps with LACS9 in Arabidopsis seed oil biosynthesis. The Plant Journal, 2010,64(6):1048-1058. |
[1] | 陈吉浩, 周界光, 曲翔汝, 王素容, 唐华苹, 蒋云, 唐力为, $\boxed{\hbox{兰秀锦}}$, 魏育明, 周景忠, 马建. 四倍体小麦胚大小性状QTL定位与分析[J]. 中国农业科学, 2023, 56(2): 203-216. |
[2] | 胡盛,李阳阳,唐章林,李加纳,曲存民,刘列钊. 干旱胁迫下甘蓝型油菜籽粒含油量和蛋白质含量变化的全基因组关联分析[J]. 中国农业科学, 2023, 56(1): 17-30. |
[3] | 唐华苹,陈黄鑫,李聪,苟璐璐,谭翠,牟杨,唐力为,兰秀锦,魏育明,马建. 基于55K SNP芯片的普通小麦穗长非条件和条件QTL分析[J]. 中国农业科学, 2022, 55(8): 1492-1502. |
[4] | 职蕾,者理,孙楠楠,杨阳,Dauren Serikbay,贾汉忠,胡银岗,陈亮. 小麦苗期铅耐受性的全基因组关联分析[J]. 中国农业科学, 2022, 55(6): 1064-1081. |
[5] | 巢成生,王玉乾,沈欣杰,代晶,顾炽明,李银水,谢立华,胡小加,秦璐,廖星. 甘蓝型油菜苗期氮高效吸收转运特征研究[J]. 中国农业科学, 2022, 55(6): 1172-1188. |
[6] | 赵凌, 张勇, 魏晓东, 梁文化, 赵春芳, 周丽慧, 姚姝, 王才林, 张亚东. 利用高密度Bin图谱定位水稻抽穗期剑叶叶绿素含量QTL[J]. 中国农业科学, 2022, 55(5): 825-836. |
[7] | 王慧玲, 闫爱玲, 孙磊, 张国军, 王晓玥, 任建成, 徐海英. 鲜食葡萄果实单萜合成关键基因的eQTL分析[J]. 中国农业科学, 2022, 55(5): 977-990. |
[8] | 谢伶俐,韦丁一,章子爽,徐劲松,张学昆,许本波. 甘蓝型油菜发育进程中赤霉素动态变化及其与产量的关系[J]. 中国农业科学, 2022, 55(24): 4793-4807. |
[9] | 李恒,字向东,王会,熊燕,吕明杰,刘宇,蒋旭东. 基于全基因组重测序的山羊产羔数性状关键调控基因的筛选[J]. 中国农业科学, 2022, 55(23): 4753-4768. |
[10] | 刘进,胡佳晓,马小定,陈武,勒思,Jo Sumin,崔迪,周慧颖,张立娜,Shin Dongjin,黎毛毛,韩龙植,余丽琴. 水稻RIL群体高密度遗传图谱的构建及苗期耐热性QTL定位[J]. 中国农业科学, 2022, 55(22): 4327-4341. |
[11] | 谢晓宇, 王凯鸿, 秦晓晓, 王彩香, 史春辉, 宁新柱, 杨永林, 秦江鸿, 李朝周, 马麒, 宿俊吉. 陆地棉吐絮率的限制性两阶段多位点全基因组关联分析及候选基因预测[J]. 中国农业科学, 2022, 55(2): 248-264. |
[12] | 邹林翰,周新颖,张泽源,蔚睿,袁梦,宋晓朋,简俊涛,张传量,韩德俊,宋全昊. 小麦周8425B×小偃81重组自交系群体千粒重相关性状的QTL定位及单倍型分析[J]. 中国农业科学, 2022, 55(18): 3473-3483. |
[13] | 常立国,何坤辉,刘建超. 多环境下玉米保绿相关性状遗传位点的挖掘[J]. 中国农业科学, 2022, 55(16): 3071-3081. |
[14] | 郭淑青,宋慧,柴少华,郭岩,石兴,杜丽红,邢璐,解慧芳,张扬,李龙,冯佰利,刘金荣,杨璞. 谷子生育期及穗相关性状的QTL定位[J]. 中国农业科学, 2022, 55(15): 2883-2898. |
[15] | 李婷,董远,张君,冯志前,王亚鹏,郝引川,张兴华,薛吉全,徐淑兔. 玉米杂交种穗部性状的全基因组关联分析[J]. 中国农业科学, 2022, 55(13): 2485-2499. |
|