以六倍体（AnAnCnCnCoCo）为桥梁创制抗旱新型甘蓝型油菜（AnArCnCo）

doi:10.3864/j.issn.0578-1752.2020.16.003

摘要/Abstract

摘要：

【目的】作为甘蓝型油菜的祖先种之一,白菜型油菜具有遗传多样性丰富、耐干旱、耐土壤瘠薄等优良特性。分析白菜型油菜的抗旱性,并以六倍体为桥梁,创造导入白菜型油菜优良抗旱性的新型甘蓝型油菜。【方法】以甘蓝型油菜和甘蓝为亲本,经杂交、胚挽救、染色体加倍,获得六倍体材料（AⁿAⁿCⁿCⁿC^oC^o）。以六倍体（AⁿAⁿCⁿCⁿC^oC^o）与白菜型油菜（A^rA^r）为亲本,杂交获得新型甘蓝型油菜（AⁿA^rCⁿC^o）;以PEG-6000溶液于萌发期模拟干旱处理新型甘蓝型油菜、白菜型油菜,测算种子萌发抗旱指数、相对萌发率、相对萌发势、相对根长和相对胚轴长,评价其抗旱性,并运用隶属函数法对抗旱性鉴定指标进行分析,筛选具有优良抗旱性的新型甘蓝型油菜。【结果】以11份六倍体材料和68份白菜型油菜为亲本,创建了124份新型甘蓝型油菜。新型甘蓝型油菜苗期表型介于六倍体和白菜型油菜之间。所选六倍体材料的染色体数目为56条,花粉育性约90%,合成的新型甘蓝型油菜的染色体数目为38条,花粉育性约80%。选取59份长势优良的新型甘蓝型油菜、7份六倍体材料、10份白菜型油菜以及20份自然甘蓝型油菜,进行主成分分析,四类材料分成三类,即六倍体材料、自然甘蓝型油菜和新型甘蓝型油菜、白菜型油菜,但自然甘蓝型油菜和新型甘蓝型油菜明显分开。以PEG-6000溶液模拟干旱,测定抗旱性指标,确定了用于白菜型油菜和新型甘蓝型油菜模拟干旱处理的PEG-6000溶液浓度,分别为200和250 g·L^-1。从59份新型甘蓝型油菜中选取9份进行抗旱性鉴定,发现其中有3份新型甘蓝型油菜的抗旱性优于对照甘蓝型油菜中双11号,而且这三份新型甘蓝型油菜的抗旱性与各自的父本白菜型油菜的抗旱性呈正相关性。【结论】以具备优良抗旱性的白菜型油菜为亲本,以六倍体材料为桥梁,可创制具备优良抗旱性的新型甘蓝型油菜。

关键词: 甘蓝型油菜, 白菜型油菜, 抗旱性, PEG-6000, 主成分分析

Abstract:

【Objective】As one parent of Brassica napus, B. rapa has a wide range of variation, as well as excellent performance against drought or poor soil. Employing hexaploid as a bridge, the drought resistance of B. rapa is transferred to develop a new-type B. napus with excellent drought tolerance.【Method】The hexaploid (AⁿAⁿCⁿCⁿC^oC^o) was created via interspecific cross between B. napus (AⁿAⁿCⁿCⁿ) and B. oleracea (C^oC^o) via embryo rescue and chrosome doubling. Next, a new-type B. napus（AⁿA^rCⁿC^o）was developed by crossing the hexaploid with B. rapa (A^rA^r). Under the treatment with PEG-6000 solution to imitate drought stress at germination stage, the indices related to drought resistance were measured in the experimental materials, including natural B. napus, new-type B. napus and B.rapa. All the datum were analyzed with subordinate function method.【Result】 Using 11 hexaploid materials and 68 B. rapa materials as parents, 124 new-type B. napus lines were created, which showed intermediate morphology between hexaploid and B. rapa. The hexaploid used in the experiment all had 56 chrosomes and the new-type B. napus had 38 chromsomes. The pollen fertility in the hexaploid and the new-type B. napus was approximately 90% and 80%, respectively. Based on the results of principal components analysis and SSR analysis, 7 hexaploid, 59 new-type B. napus, 10 B. rapa and 20 natural B. napus were clustered into three groups, namely B.rapa, hexaploid and B. napus. The new-type B. napus and natural B. napus were in the same group, but they clearly separated each other. Based on the drought resistance-related indices, 200 g·L^-1 and 250 g·L^-1 PEG-6000 solution were used to imitate drought stress to B. rapa and new-type B. napus, respectively. Nine new-type B. napus were tested, but only three were more resistant than the control, Zhongshuang 11. Additionally, the resistance were positively associated to the corresponding parent B. rapa, which indicated that the three new-type B. napus have been transfered excellent drought resistance from B. rapa.【Conclusion】 Using B. rapa as a drought resistance donor and the hexaploid as a bridge, new-type B. napus lines with excellent drought tolerance were developed.

Key words: Brassica napus, Brassica rapa, drought tolerance, PEG-6000, principal components analysis

万华方,魏帅,冯宇霞,钱伟. 以六倍体（AⁿAⁿCⁿCⁿC^oC^o）为桥梁创制抗旱新型甘蓝型油菜（AⁿA^rCⁿC^o）[J]. 中国农业科学, 2020, 53(16): 3225-3234.

WAN HuaFang,WEI Shuai,FENG YuXia,QIAN Wei. Creating a New-Type Brassica napus (AⁿA^rCⁿC^o) with High Drought-resistance Employing Hexaploid (AⁿAⁿCⁿCⁿC^oC^o) as a Bridge[J]. Scientia Agricultura Sinica, 2020, 53(16): 3225-3234.

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参考文献 42

[1]	NAGAHARU U. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Japan Journal of Botany, 1935,7:389-452.
[2]	CHALHOUB B, DENOEUD F, LIU S Y, PARKIN I A P, TANG H B, WANG X Y, CHIQUET J, BELCRAM H, TONG C B, SAMANS B, CORRÉA M, DA SILVA C, JUST J, FALENTIN C, KOH C S, LE CLAINCHE I, BERNARD M, BENTO P, NOEL B, LABADIE K, ALBERTI A, CHARLES M, ARNAUD D, GUO H, DAVIAUDC ALAMERY S, JABBARI K, ZHAO M X, EDGER P P, CHELAIFA H, TACK D, LASSALLE G, MESTIRI I, SCHNEL N, LE PASLIER M C, FAN G Y, RENAULT V, BAYER P E, GOLICZ A A, MANOLI S, LEE T H, THI D V H, CHALABI S, HU Q, FAN C C, TOLLENAERE R, LU Y H, BATTAIL C, SHEN J X, SIDEBOTTOM C H D, WANG X F, CANAGUIER A, CHAUVEAU A, BÉRARD A, DENIOT G, GUAN M, LIU Z S, SUN F M, LIM Y P, LYONS E, TOWN C D, BANCROFT I, WANG X W, MENG J L, MA J X, PIRES J C, KING G J, BRUNEL D, DELOURME R, RENARD M, AURY J M, ADAMS K L, BATLEY J, SNOWDON R J, TOST J, EDWARDS D, ZHOU Y M, HUA W, SHARPE A G, PATERSON A H, GUAN C Y, WINCKER P. Early allopolyploid evolution in the post- Neolithic Brassica napus oilseed genome. Science, 2014,345(6199):950-953. doi: 10.1126/science.1253435 pmid: 25146293
[3]	PRAKASH S, WU X, BHAT SR. History, evolution and domestication of Brassica crops. Plant Breeding Reviews, 2012,35:19-84.
[4]	VALLIYODAN B, NGUYEN H T. Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Current Opinion in Plant Biology, 2006,9(2):189-195. pmid: 16483835
[5]	TESTER M, LANGRIDGE P. Breeding technologies to increase crop production in a changing world. Science, 2010,327:818-822. pmid: 20150489
[6]	白鹏, 冉春燕, 谢小玉. 干旱胁迫对油菜蕾薹期生理特性及农艺性状的影响. 中国农业科学, 2014,47(18):3566-3776. doi: 10.3864/j.issn.0578-1752.2014.18.005
	BAI P, RAN C Y, XIE X Y. Influence of drought stress on physiological characteristics and agronomic traits at bud stage of rapeseed (Brassica napus L.). Scientia Agricultural Sinica, 2014,47(18):3566-3776. (in Chinese) doi: 10.3864/j.issn.0578-1752.2014.18.005
[7]	BECILER H C, ENGQVIST G M, ILARLSSON B. Comparison of rapeseed cultivars and resynthesized lines based on allozyme and RFLP markers. Theoretical and Applied Genetics, 1995,91(1):62-67. pmid: 24169668
[8]	ALLENDER C, KING G. Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers. BMC Plant Biology, 2010,10:54-62. pmid: 20350303
[9]	MEI J Q, LI Q F, QIAN L W, FU Y, LI J N, FRAUEN M, QIAN W. Genetic investigation of the origination of allopolyploid with virtually synthesized lines: application to the C subgenome of Brassica napus. Heredity, 2011,106(6):955-961. pmid: 21102622
[10]	BUS A, KÖRBER N, SNOWDON R J, STICH B. Patterns of molecular variation in a species-wide germplasm set of Brassica napus. Theoretical and Applied Genetics, 2011,123:1413-1423. pmid: 21847624
[11]	QIAN W, MENG J L, LI M T, FRAUEN M, SASS O, NOACIL J, JUNG C. Introgression of genomic components from Chinese Brassica rapa contributes to widening the genetic diversity in rapeseed(B. napus L.) with emphasis on the evolution of Chinese rapeseed. Theoretical and Applied Genetics, 2006,113:49-54. pmid: 16604336
[12]	GIRILE A, SCHIERHOLT A, BECILER H C. Extending the rapeseed gene pool with resynthesized Brassica napus I: Genetic diversity. Genetic Resources and Crop Evolution, 2012,59:1441-1447.
[13]	GIRILE A, SCHIERHOLT A, BECILER H C. Extending the rapeseed gene pool with resynthesized Brassica napns II: Heterosis. Theoretical and Applied Genetics, 2012,124(6):1017-1026. doi: 10.1007/s00122-011-1765-7
[14]	FU D H, QIAN W, ZOU J, MENG J L. Genetic dissection of inter- subgenomic heterosis in Brassica napus carrying genomic components of B. rapa. Euphytica, 2012,184:151-164. doi: 10.1007/s10681-011-0533-8
[15]	ZOU J, HU D D, MASON A S, SHEN X Q, WANG X H, WANG N, GRANDKE F, WANG M, CHAN S H, SNOWDON R J, MENG J L. Genetic changes in a novel breeding population of Brassica napus synthesized from hundreds of crosses between B. rapa and B. carinata. Plant Biotechnology Journal, 2018,16(2):507-519. pmid: 28703467
[16]	QIAN L W, QIAN W, SNOWDON R J. Sub-genomic selection patterns as a signature of breeding in the allopolyploid Brassica napus genome. BMC Genomics, 2014,15(1):1170-1186.
[17]	TALEBI R, HAGHNAZARI A, TABATABAEI I. Assessment of genetic variation within international collection of Brassica rapa genotypes using inter simple sequence repeat DNA markers. Biharean Biologist, 2010,4(2):145-153.
[18]	ZHAO J J, WANG X W, DENG B, LOU P, WU J, SUN R F, XU Z Y, VROMANS J, KOORNNEEF M, BONNEMA G. Genetic relationships within Brassica rapa as inferred from AFLP fingerprints. Theoretical and Applied Genetics, 2005,110(7):1301-1314. doi: 10.1007/s00122-005-1967-y pmid: 15806345
[19]	GUO Y M, CHEN S, LI Z Y, COWLING W A. Centre of origin and centres of diversity in an ancient crop,Brassica rapa (turnip rape). Journal of Heredity, 2014,105(4):555-565. pmid: 24714366
[20]	何余堂, 陈宝元, 傅廷栋, 李殿荣, 涂金星. 白菜型油菜在中国的起源与进化. 遗传学报, 2003,30(11):1003-1012.
	HE Y T, CHEN B Y, FU T D, LI D R, TU J X. Origin and evolution of Brassica campestris L. in China. Acta Genetica Sinica, 2003,30(11):1003-1012. (in Chinese)
[21]	GUO Y M, TURNER N C, CHEN S, NELSON M N SIDDIQUE K H M, COWLING W A. Genotypic variation for tolerance to transient drought during the reproductive phase of Brassica rapa. Journal of Agronomy and Crop Science, 2015,201(4):267-279. doi: 10.1111/jac.12107
[22]	CHEN S, ZOU J, COWLING W A, MENG J L. Allelic diversity in a novel gene pool of canola-quality Brassica napus enriched with alleles from B. rapa and B. carinata. Crop and Pasture Science, 2010,61(6):483-492.
[23]	GUO Y M, SAMANS B, CHEN S, KIBRET K B, HATZIG S, TURNER N C, NELSON M N, COWLING W A, SNOWDON R J. Drought-tolerant Brassica rapa shows rapid expression of gene networks for general stress responses and programmed cell death under simulated drought Stress. Plant Molecular Biology Report, 2017,35(4):416-430.
[24]	周庆红, 周灿, 范淑英. 远缘杂交在芸薹属作物育种中的应用研究进展. 北方园艺, 2015(2):165-170.
	ZHOU Q H, ZHOU C, FAN S Y. Research advance in application of distant hybridization on breeding of Brassica crops. Northern Horticulture, 2015(2):165-170. (in Chinese)
[25]	RIPLEY V L, BEVERSDORF W D. Development of self- incompatible Brassica napus:(I) Introgression of S alleles from Brassica oleracea through interspecific hybridization. Plant Breeding, 2010,122(1):1-5.
[26]	岳芳, 汪雷, 陈燕桂, 忻晓霞, 李勤菲, 梅家琴, 熊志勇, 钱伟. 利用异源六倍体(A^rA^rAⁿAⁿCⁿCⁿ)与甘蓝种间杂交合成甘蓝型油菜的新方法. 作物学报, 2019,45(2):188-195.
	YUE F, WANG L, CHEN Y G, JIN X X, LI Q F, MEI J Q, XIONG Z Y, QIAN W. A new method for the synthesis of Brassica napus by using inter-species hexaploid (A^rA^rAⁿAⁿCⁿCⁿ) and B. oleracea. Acta Agronomica Sinica, 2019,45(2):188-195. (in Chinese)
[27]	钱伟, 李勤菲, 梅家琴, 付东辉, 李加纳. 一种利用白菜型油菜拓宽甘蓝型油菜遗传变异的方法:中国, 2010106071854.3.2013.
	QIAN W, LI Q F, MEI J Q, FU D H, LI J N. A strategy of using Brassica rapa to widen genetic variance of B. napus: China , 201010607185. 4.2013. (in Chinese)
[28]	MEI J Q, LIU Y, WEI D Y, WITTKOP B, DING Y J, LI Q F, LI J N, WAN H F, LI Z Y, GE X H, FRAUEN M, SNOWDON , R J, QIAN W, FRIENT W. Transfer of sclerotinaia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step. Theoretical and Applied Genetics, 2015,128(4):639-644. doi: 10.1007/s00122-015-2459-3 pmid: 25628163
[29]	李勤菲, 陈致富, 刘瑶, 梅家琴, 钱伟. 六倍体(AⁿAⁿCⁿCⁿC^oC^o)与白菜型油菜杂交可交配性及后代菌核病抗性. 中国农业科学, 2017,50(1):123-133. doi: 10.3864/j.issn.0578-1752.2017.01.011
	LI Q F, CHEN Z F, LIU Y, MEI J Q, QIAN W. Crossability and sclerotinia resistance among hybrids between hexaploid (AⁿAⁿCⁿCⁿC^oC^o) and Brassica rapa. Scientia Agricultura Sinica, 2017,50(1):123-133.( in Chinese) doi: 10.3864/j.issn.0578-1752.2017.01.011
[30]	SHANGGUAN Z P, LEI T W, SHAO M A, XUE Q W. Effects of phosphorus nutrient on the hydraulic conductivity of Sorghum (Sorghum vulgare Pers.) seedling roots under water deficiency. Journal of Integrative Plant Biology, 2005,47(4):421-427.
[31]	陈郡雯, 吴卫, 郑有良, 侯凯, 徐应文, 翟娟园. 聚乙二醇(PEG-6000)模拟干旱条件下白芷苗期抗旱性研究. 中国中药杂志, 2010,35(2):149-153. pmid: 20394281
	CHEN J W, WU W, ZHENG Y L, HOU K, XU Y W, ZHAI J Y. Drought resistance of Angelica dahurica during seedling stage under polyethylene glycol (PEG-6000)-simulated drought stress. China Journal of Chinese Materia Medica, 2010,35(2):149-153. (in Chinese) pmid: 20394281
[32]	LI Q F, ZHOU Q H, MEI J Q, ZHANG Y J, LI J N, LI Z Y, GE X H, XIONG Z Y, HUANG Y J, QIAN W. Improvement of Brassica napus via interspecific hybridization between B. napus and B. oleracea. Molecular Breeding, 2014,34(4):1955-1963. doi: 10.1007/s11032-014-0153-9
[33]	QUAZI M. Interspecific hybrids between B. napus and B. oleracea developed by embryo culture. Theoretical and Applied Genetics, 1988,75(2):309-318. doi: 10.1007/BF00303970
[34]	DING Y J, MEI J Q, LI Q F, LIU Y, WAN H F, WANG L, BECKER H C, QIAN W. Improvement of Sclerotinia sclerotiorum resistance in Brassica napus by using B. oleracea. Genetic Resources and Crop Evolution, 2013,60(5):1615-1619. doi: 10.1007/s10722-013-9978-z
[35]	XIAO Y, CHEN L L, ZOU J, TIAN E T, XIA W, MENG J L. Development of a population for substantial new type Brassica napus diversified at both A/C genomes. Theoretical Applied Genetics, 2010,121(6):1141-1150.
[36]	刘瑶, 丁一娟, 汪雷, 万华方, 梅家琴, 钱伟. 甘蓝型油菜与AⁿAⁿCⁿCⁿC^oC^o六倍体可交配性及杂种菌核病抗性. 中国农业科学, 2015,48(24):4885-4891.
	LIU Y, DING Y J, WANG L, WAN H F, MEI J Q, QIAN W. Crossability between Brassica napus with hexaploid AⁿAⁿCⁿCⁿC^oC^o and sclerotinia resistance in the hybrids. Scientia Agricultura Sinica , 2015,48(24):4885-4891. (in Chinese)
[37]	FANG Y J, XIONG L Z. General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and Molecular Life Sciences, 2015,72(4):673-689. pmid: 25336153
[38]	SHINOZAKI K, YAMAGUCHI-SHINOZAKI K. Gene networks involved in drought stress response and tolerance. Journal of Experimental Botany, 2007,58(2):221-227. doi: 10.1093/jxb/erl164 pmid: 17075077
[39]	SHINOZAKI K, YAMAGUCHI-SHINOZAKI K, SEKI M. Regulatory network of gene expression in the drought and cold stress responses. Current Opinion in Plant Biology, 2003,6(5):410-417. pmid: 12972040
[40]	卢坤, 张琳, 曲存民, 梁颖, 唐章林, 李加纳. 利用RNA-Seq鉴定甘蓝型油菜叶片干旱胁迫应答基因. 中国农业科学, 2015,48(4):630-645. doi: 10.3864/j.issn.0578-1752.2015.04.02
	LU K, ZHANG L, QU C M, LINAG Y, TANG Z L, LI J N. Identification of drought stress-responsive genes in leaves of Brassica napus by RNA sequencing. Scientia Agricultura Sinica, 2015,48(4):630-645. (in Chinese) doi: 10.3864/j.issn.0578-1752.2015.04.02
[41]	CHEN L, REN F, ZHONG H, JIANG W M, LI X B. Identification and expression analysis of genes in response to high-salinity and drought stresses in Brassica napus. Acta Biochimica et Biophysica Sinica, 2010,42(2):154-164. doi: 10.1093/abbs/gmp113 pmid: 20119627
[42]	ZHANG J, MASON A S, WU J, LIU S, ZHANG X C, LUO T, REDDEN R, BATLEY J, HU L Y, YAN G J. Identification of putative candidate genes for water stress tolerance in Canola (Brassica napus). Frontiers in Plant Science, 2015,6:1058. pmid: 26640475

编号 Code	类型 Type	萌发抗旱指数 DRI	相对萌发率 RGR	相对萌发势 RSP	相对根长 RRL	相对胚轴长 RSL
9X006	新型甘蓝型油菜New-type B. napus	0.262	0.067	0.069	0.209	0.106
9X002	新型甘蓝型油菜New-type B. napus	0.543	0.067	0.000	0.690	0.133
9X025	新型甘蓝型油菜New-type B. napus	0.798	0.300	0.296	0.718	0.135
9X016	新型甘蓝型油菜New-type B. napus	0.392	0.600	0.615	0.319	0.173
9X018	新型甘蓝型油菜New-type B. napus	0.824	0.900	0.827	0.692	0.249
9X111	新型甘蓝型油菜New-type B. napus	0.483	0.633	0.393	0.748	0.162
9X028	新型甘蓝型油菜New-type B. napus	0.591	0.933	0.933	0.156	0.109
9X015	新型甘蓝型油菜New-type B. napus	0.637	0.600	0.533	0.228	0.151
9X026	新型甘蓝型油菜New-type B. napus	0.380	0.700	0.633	0.122	0.109
中双11 ZS11	甘蓝型油菜B. napus	0.800	0.867	0.700	0.185	0.117
8M664	白菜型油菜B. rapa	0.800	0.830	0.860	0.690	0.210
8M681	白菜型油菜B. rapa	0.670	0. 830	0.800	0.920	0.410
8M625	白菜型油菜B. rapa	0.530	0.570	0.530	0.310	0.260
8M623	白菜型油菜B. rapa	0.520	0.900	0.830	0.610	0.500
8M684	白菜型油菜B. rapa	0.290	0.470	0.410	0.560	0.130
8M624	白菜型油菜B. rapa	0.370	0.700	0.500	0.510	0.210
8M693	白菜型油菜B. rapa	0.450	0.730	0.730	0.690	0.300
8M191	白菜型油菜B. rapa	0.300	0.600	0.480	0.500	0.810
8M655	白菜型油菜B. rapa	0.420	0.730	0.660	0.690	0.250

主成分 Principal component	类别 Category	特征值 Eigen value	贡献率Contribution ratio (%)	累计贡献率Accumulated contribution ratio (%)	抗旱指标特征向量Eigen vector of measured indicators
主成分 Principal component	类别 Category	特征值 Eigen value	贡献率Contribution ratio (%)	累计贡献率Accumulated contribution ratio (%)	萌发抗旱指数DRI	相对萌发率RGR	相对萌发势RSP	相对根长RRL	相对胚轴长RSL
PC1	新型甘蓝型油菜 New-type B. napus	2.431	48.620	48.620	0.416	0.597	0.589	-0.024	0.350
PC2	新型甘蓝型油菜 New-type B. napus	1.774	35.470	84.090	0.332	-0.224	-0.274	0.720	0.497
PC3	新型甘蓝型油菜 New-type B. napus	0.597	11.930	96.020	-0.784	0.083	0.110	0.011	0.605
PC1	白菜型油菜 B. rapa	3.580	71.610	71.600	0.459	0.512	0.520	0.468	0.194
PC2	白菜型油菜 B. rapa	0.950	19.010	90.600	-0.313	0.08	-0.073	-0.038	0.946
PC3	白菜型油菜 B. rapa	0.320	6.430	97.000	0.599	-0.001	0.103	-0.775	0.175

编号 Code	类型 Type	萌发抗旱指数 DRI	相对萌发率 RGR	相对萌发势RSP	相对根长 RRL	相对胚轴长 RSL	均值 Means
9X006	新型甘蓝型油菜New-type B. napus	1.00	0.00	0.01	0.73	0.20	0.388
9X002	新型甘蓝型油菜New-type B. napus	0.78	0.09	0.00	1.00	0.19	0.412
9X025	新型甘蓝型油菜New-type B. napus	1.00	0.25	0.24	0.88	0.00	0.474
9X026	新型甘蓝型油菜New-type B. napus	0.46	1.00	0.89	0.02	0.00	0.474
9X028	新型甘蓝型油菜New-type B. napus	0.58	1.00	1.00	0.05	0.00	0.526
9X111	新型甘蓝型油菜New-type B. napus	0.55	0.80	0.39	1.00	0.00	0.548
中双11 ZS11	甘蓝型油菜B. napus	0.91	1.00	0.78	0.09	0.00	0.556
9X016	新型甘蓝型油菜New-type B. napus	0.50	0.96	1.00	0.33	0.00	0.558
9X015	新型甘蓝型油菜New-type B. napus	1.00	0.92	0.78	0.15	0.00	0.570
9X018	新型甘蓝型油菜New-type B. napus	0.88	1.00	0.89	0.68	0.00	0.690
8M623	白菜型油菜B. rapa	0.07	1.00	0.83	0.28	0.00	0.436
8M191	白菜型油菜B. rapa	0.00	0.61	0.35	0.40	1.00	0.472
8M624	白菜型油菜B. rapa	0.31	1.00	0.59	0.62	0.00	0.504
8M684	白菜型油菜B. rapa	0.37	0.78	0.66	1.00	0.00	0.562
8M625	白菜型油菜B. rapa	0.88	1.00	0.89	0.17	0.00	0.588
8M681	白菜型油菜B. rapa	0.51	0.83	0.76	1.00	0.00	0.620
8M655	白菜型油菜B. rapa	0.35	1.00	0.84	0.91	0.00	0.620
8M693	白菜型油菜B. rapa	0.36	1.00	1.00	0.90	0.00	0.652
8M664	白菜型油菜B. rapa	0.90	0.96	1.00	0.73	0.00	0.721

编号Code	9X006	9X002	9X025	9X026	9X028	9X111	中双11 ZS 11	9X016	9X015	9X018
8M623	-0.686	-0.583	-0.336	0.874*	0.833*	0.354	0.610	0.909*	0.522	0.666
8M191	-0.631	-0.497	-0.821*	-0.274	-0.362	-0.475	-0.563	-0.398	-0.665	-0.734
8M624	-0.309	-0.129	0.081	0.692	0.651	0.764	0.560	0.791	0.503	0.789
8M684	0.023	0.289	0.398	0.311	0.300	0.912*	0.216	0.539	0.201	0.695
8M625	-0.151	-0.317	0.122	0.923**	0.948**	0.265	0.992**	0.891*	0.986**	0.872*
8M681	0.064	0.28	0.455	0.400	0.402	0.906*	0.341	0.622	0.347*	0.793
8M655	-0.232	-0.005	0.177	0.569	0.547	0.805	0.409	0.748	0.372	0.775
8M693	-0.291	-0.089	0.122	0.624	0.611	0.721	0.440	0.805	0.404	0.784
8M664	0.067	0.052	0.441	0.742	0.778	0.651	0.800	0.864*	0.818*	0.991**

以六倍体（AⁿAⁿCⁿCⁿC^oC^o）为桥梁创制抗旱新型甘蓝型油菜（AⁿA^rCⁿC^o）

Creating a New-Type Brassica napus (AⁿA^rCⁿC^o) with High Drought-resistance Employing Hexaploid (AⁿAⁿCⁿCⁿC^oC^o) as a Bridge

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