北方强冬性甘蓝型冬油菜品种(系)抗寒性评价

doi:10.3864/j.issn.0578-1752.2019.19.002

中国农业科学 ›› 2019, Vol. 52 ›› Issue (19): 3291-3308.doi: 10.3864/j.issn.0578-1752.2019.19.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

北方强冬性甘蓝型冬油菜品种(系)抗寒性评价

蒲媛媛,赵玉红,武军艳,刘丽君,白静,马骊,牛早霞,金姣姣,方彦,李学才,孙万仓()

甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省油菜工程技术研究中心/甘肃省作物遗传改良与种质创新重点实验室,兰州 730070

收稿日期:2019-04-19 接受日期:2019-05-30 出版日期:2019-10-01 发布日期:2019-10-11
通讯作者: 孙万仓
作者简介:蒲媛媛,E-mail：vampirepyy@126.com。
基金资助:
国家现代农业产业技术体系(CARS-12);国家“973”计划(2015CB150206);科技部项目(2016YFD0101300);甘肃省重大专项(17ZD2NA016-4);甘肃省现代农业产业技术体系(GARS-TSZ-1)

Comprehensive Assessment on Cold Tolerance of the Strong Winter Brassica napus L. Cultivated in Northern China

PU YuanYuan,ZHAO YuHong,WU JunYan,LIU LiJun,BAI Jing,MA Li,NIU ZaoXia,JIN JiaoJiao,FANG Yan,LI XueCai,SUN WanCang()

College of Agronomy, Gansu Agricultural University/Gansu Provincial Key Laboratory of Arid Land Crop Sciences/Gansu Research Center of Rapeseed Engineering and Technology/Key Laboratory of Crop Genetics Improvement and Germplasm Enhancement of Gansu Province, Lanzhou, 730070

Received:2019-04-19 Accepted:2019-05-30 Online:2019-10-01 Published:2019-10-11
Contact: WanCang SUN

摘要/Abstract

摘要：

【目的】 通过对12个北方强冬性甘蓝型油菜的抗寒性进行比较,并运用3种鉴定方法对其抗寒性进行明确划分,为北方甘蓝型冬油菜抗寒性改良提供科学可靠的鉴定方法及优良的抗寒种质。【方法】 以12个北方强冬性甘蓝型油菜为材料,通过观察记载冬前植株形态、统计田间越冬率、计算半致死温度（LT₅₀）、测定冬前低温下生理指标和比较分析冬油菜春播后品种（系）间生长发育的差异、春化率的差异与抗寒性的关系,来比较分析品种间抗寒性的差异,接着运用LT₅₀、隶属函数综合评价法和春化差异比较的方法对参试材料的抗寒性强弱进一步进行了明确划分。【结果】 参试材料在甘肃天水（34°60′N,海拔1 084—1 650 m）越冬率为92.1%—97.8%,北移至兰州（36°73′N、海拔1 517 m）和上川（36°03′N、海拔2 150 m）,越冬率大幅度降低,兰州越冬率为36.0%—78.6%（地膜覆盖）,上川越冬率仅为0—14.4%（地膜覆盖）,甘肃农业大学新育成的16TS 309-4、16TS 306-3、16TS 309-10、15NS 45-4、2016 8(G)和2016TSG（10）强冬性甘蓝型冬油菜品系,平均越冬率为10.2%—14.4%（上川）。上述品系越冬前植株生长习性趋于匍匐生长,心叶色和幼茎色呈黄绿色或紫色,叶片颜色深绿,地下部干物质积累大于地上部,根冠比增加,介于0.23—0.95,且差异显著（P＜0.05）。低温条件下,叶片的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）酶活性、可溶性蛋白质（soluble protein）、可溶性糖（soluble sugar）和游离脯氨酸（proline）含量相对较高,且LT₅₀较低,在-13.4—-5.7℃。冬油菜春播后,12个参试材料的田间春化率介于4.05%—87.65%,2016TS(G)10春化率最低,为4.05%,小区平均株高为10.77 cm,未现蕾阶段的植株薹高10.50 cm,现蕾阶段的植株薹高17.10 cm,均为品种（系）间最低。相关性分析表明,春化率与平均株（薹）高、成熟期植株所占比例和LT₅₀极显著正相关,相关系数达0.90—0.96,与越冬率、综合评价(D)值、CAT、POD、SP呈极显著负相关,相关系数为-0.96—-0.63。【结论】 在中国北方,冬油菜适时春播,可通过田间春化率的差异、植株生育时期的差异及平均株（薹）高的差异来评价冬油菜抗寒性的强弱。甘肃农业大学新育成的7个甘蓝型冬油菜品系,在北纬36°03′,海拔2 150 m的地区可以越冬,抗寒性显著优于天油14和天油2288（天水市农业科学研究所选育）及新油23（新疆农业科学院选育）。

关键词: 甘蓝型油菜, 越冬率, 半致死温度, 春化率, 抗寒性评价

Abstract:

【Objective】 In this study, the cold tolerance of twelve strong winter Brassica napus, which planted in northern China, were compared. The degree of cold tolerance was clearly classified by three identification methods. It provides a scientific and reliable identification method and varieties (lines) with strong cold tolerance, for improvement of Brassica napus in northern China. 【Method】 Twelve strong winter Brassica napus varieties (lines) were used as the materials. In order to compare and analyze the difference of cold tolerance among these materials, we observed the morphology of seedlings before overwintering, recorded statistical overwintering rate and calculated the lethal temperature (LT₅₀), meanwhile, measured physiological indexes, and analyzed the relationship among cold tolerance, vernalization rate and the differences in growth stages after sowing in early spring. Then, we used LT₅₀, subordinate function and comparison of vernalization differences, respectively, to clarify the cold tolerance classification of twelve Brassica napus. 【Result】 The overwintering survival rate of materials in Tianshui area (34°60′N, altitude 1 084-1 650 m) was 92.1%-97.8%. However, the overwintering survival rate decreased rapidly after moving to Lanzhou (36°73′N, altitude 1 517 m) and Shangchuan (36°03′N, altitude 2 150 m), the overwintering survival rate was 0-14.4%, (plastic film mulch) and 36.0%-78.6% (plastic film mulch) respectively. The strong winter Brassica napus lines ‘16TS 309-4, 16TS 306-3, 16TS 309-10, 15NS 45-4, 2016 8(G) and 2016TSG (10)’ which were new bred by Gansu Agricultural University could survive over winter, with the average overwintering survival rate of 10.2%-14.4%. Before overwintering, the seedlings of these new materials tended to prostrate growth, the colors of heart leaves and young stems were yellow-green or purple, the color of leaves was dark green, the accumulation of underground dry matter was greater than aboveground dry matter, and the root shoot ratio increased which was between 0.23 to 0.95, with a significant difference (P＜0.05). Under cold stress, leaves kept relatively high enzyme activity of super oxide dismutase (SOD), peroxidase (POD) and catalase (CAT), moreover, the high content of soluble protein (SP), soluble sugar (SS) and free proline (Pro) was detected, and LT₅₀ was relatively lower (range from -13.4℃ to -5.7℃). In the field experiment, twelve winter rapeseeds were sowed in early spring, the results have shown that the vernalization rate was between 4.05% and 87.65%, and ‘2016TS (G) 10’ was the lowest one. However, ‘2016TS (G) 10’ not only has the lowest vernalization rate (4.05%), but also the average plant height (10.77 cm), the average blot height of plants in the not budding stage (10.50 cm) and the average blot height of plants in budding stage (17.10 cm) were all the lowest one. Correlation analysis showed that the vernalization rate was significantly positively correlated with the average plant (bolt) height, the proportion of mature plants and LT₅₀, with R ²of 0.90-0.96, and significantly negatively correlated with overwintering survival rate, LT₅₀, comprehensive evaluation value (D), CAT, POD and SP, with R ² of -0.96--0.63.【Conclusion】 In northern China, sowing winter rapeseeds in the early spring, according to the difference of vernalization rate, growth stage and average plant (blot) high, the cold tolerance of winter rapeseeds were evaluated. The seven new lines of Brassica napus bred by Gansu Agricultural University showed strong cold tolerance, could over winter in the area of 36°03′N, altitude 2 150 m, and the cold tolerance was significantly strong than Tianyou 14, Tianyou 2288 (bred by Tianshui Agricultural Science Research Institute) and Xinyou 23 (bred by Xinjiang Academy of Agricultural Sciences), that is an essential germplasm resource with strong cold tolerance for Brassica napus breeding in northern China.

Key words: Brassica napus L., overwintering survival rate, LT₅₀, vernalization rate, assessment of cold tolerance

蒲媛媛,赵玉红,武军艳,刘丽君,白静,马骊,牛早霞,金姣姣,方彦,李学才,孙万仓. 北方强冬性甘蓝型冬油菜品种(系)抗寒性评价[J]. 中国农业科学, 2019, 52(19): 3291-3308.

PU YuanYuan,ZHAO YuHong,WU JunYan,LIU LiJun,BAI Jing,MA Li,NIU ZaoXia,JIN JiaoJiao,FANG Yan,LI XueCai,SUN WanCang. Comprehensive Assessment on Cold Tolerance of the Strong Winter Brassica napus L. Cultivated in Northern China[J]. Scientia Agricultura Sinica, 2019, 52(19): 3291-3308.

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参考文献 51

[1]	孙万仓, 武军艳, 方彦, 刘秦, 杨仁义, 马维国, 李学才, 张俊杰, 张鹏飞, 曹建明, 孙佳 . 北方旱寒区北移冬油菜生长发育特性. 作物学报, 2010,36(12):2124-2134. doi: 10.3724/SP.J.1006.2010.02124
	SUN W C, WU J Y, FANG Y, LIU Q, YANG R Y, MA W G, LI X C, ZHANG J J, ZHANG P F, CAO J M, SUN J . Growth and development characteristics of winter rapeseed northern-extended from the cold and arid regions in China. Acta Agronomica Sinica, 2010,36(12):2124-2134. (in Chinese) doi: 10.3724/SP.J.1006.2010.02124
[2]	王晨光, 李思训 . 陕西省甘蓝型油菜再次北移的探讨. 陕西农业科学(自然科学版), 2000(3):32-33, 46.
	WANG C G, LI S X . Study on the north extension ofBrassica napus. Shaanxi Journal of Agricultural Sciences (Natural Science Edition), 2000(3):32-33, 46. (in Chinese)
[3]	殷艳, 廖星, 余波, 王汉中 . 我国油菜生产区域布局演变和成因分析. 中国油料作物报, 2010,32(1):147-151.
	YIN Y, LIAO X, YU B, WANG H Z . Regional distribution evolvement and development tendency of Chinese rapeseed production. Chinese Journal of Oilcrop Sciences, 2010,32(1):147-151. (in Chinese)
[4]	雷建明, 庞进平, 范提平, 张建学, 张岩, 张亚宏 . 强冬性甘蓝型单低杂交油菜02N杂2的选育. 中国种业, 2010(6):66-67.
	LEI J M, PANG J P, FAN T P, ZHANG J X, ZHANG Y, ZHANG Y H . Breeding of strong winter type hybrid rapeseed ‘02N hybrid 2’. Chinese Seed Industry, 2010(6):66-67. (in Chinese)
[5]	黄继英 . 甘蓝型冬油菜北移可行性初探. 中国油料, 1992(4):43-46.
	HUANG J Y . Preliminary study on the feasibility of north migration ofBrassica napus. Chinese Oil, 1992(4):43-46. (in Chinese)
[6]	宋良图 . 甘蓝型油菜抗寒性的形态生理学研究. 安徽农业科学, 1992(2):127-132.
	SONG L T . Morphological and physiological basis of the cold hardiness of winter rape( B. napus). Journal of Anhui Agricultural Sciences, 1992(2):127-132. (in Chinese)
[7]	史鹏辉 . 北方强冬性白菜型冬油菜抗寒性评价及抗寒机制研究[D]. 兰州: 甘肃农业大学, 2013: 14-15.
	SHI P H . Evaluation of cold tolerance and study on the cold tolerance mechanism on strong anti-cold north winter rapeseed (Brassica rapa)[D]. Lanzhou: Gansu Agricultural University, 2013: 14-15. (in Chinese)
[8]	马骊, 孙万仓, 刘自刚, 赵艳宁, 杨刚, 刘海卿, 武军艳, 方彦, 李学才, 刘林波, 钱武, 侯献飞 . 白菜型与甘蓝型冬油菜抗寒机理差异的研究. 华北农学报, 2016(1):147-154.
	MA L, SUN W C, LIU Z G, ZHAO Y N, YANG G, LIU H Q, WU J Y, FANG Y, LI X C, LIU L B, QIAN W, HOU X F . Study of difference in mechanism of cold resistance of winter rapeseed ofBrassica rape and Brassica napus. Acta Agriculturae Boreali-Sinica, 2016(1):147-154. (in Chinese)
[9]	杜春芳 . 甘蓝型油菜低温诱导的转录组和蛋白组分析[D]. 武汉: 华中农业大学, 2016: 29-32.
	DU C F . Analysis of transcriptomics and proteomics induced by cold stress in Brassica napus L.[D]. Wuhan: Huazhong Agricultural University, 2016: 29-32. (in Chinese)
[10]	王月, 孙万仓, 刘自刚, 杨宁宁, 方彦, 曾秀存, 孔德晶, 鲁美宏, 王丽萍, 董红业, 杨刚, 侯献飞, 刘林波, 种彦容 . 甘蓝型冬油菜在西北不同生态区适应性及生理生化反应. 干旱地区农业研究, 2015,33(4):197-205.
	WANG Y, SUN W C, LIU Z G, YANG N N, FANG Y, ZENG X C, KONG D J, LU M H, WANG L P, DONG H Y, YANG G, HOU X F, LIU L B, ZHONG Y R . Adaptation and physiological and biochemical characteristics of winter rapeseed ( Brassica napus L.) in different eco-regions of northwest China. Agricultural Research in the Arid Areas, 2015,33(4):197-205. (in Chinese)
[11]	孙万仓, 武军艳, 曾军, 朱慧霞, 刘雅丽, 张亚宏 . 8个白菜型冬油菜品种抗寒性的初步评价. 湖南农业大学学报(自然科学版), 2007,33(8):151-155.
	SUN W C, WU J Y, ZENG J, ZHU H X, LIU L Y, ZHANG Y H . Preliminary evaluation of cold resistance of 8 Chinese cabbage varieties. Journal of Hunan Agricultural University (Natural Science Edition), 2007,33(8):151-155. (in Chinese)
[12]	张晓红, 冯梁杰, 杨特武, 徐正华, 胡立勇 . 冬季低温胁迫对油菜抗寒生理特性的影响. 植物生理学报, 2015,51(5):737-746.
	ZHANG X H, FENG L J, YANG T W, XU Z H, HU L Y . Effects of chilling stress on physiological characteristics of rapeseed seedlings in winter. Plant Physiology Journal, 2015,51(5):737-746. (in Chinese)
[13]	闫蕾, 蔡俊松, 高立兵, 黄斌, 马海清, 刘清云, 戴熙燕, 张学昆, 程勇, 邹锡玲 . 甘蓝型油菜抗寒性鉴定方法的建立和种质资源筛选. 中国油料作物学报, 2018,40(1):74-83.
	YAN L, CAI J S, GAO L B, HUANG B, MA H Q, LIU Q Y, DAI X Y, ZHANG X K, CHENG Y, ZOU X L . Identification method and selection of cold tolerance in rapeseed ( Brassica napus L.). Chinese Journal of Oil Crop Sciences, 2018,40(1):74-83. (in Chinese)
[14]	杨宁宁, 孙万仓, 刘自刚, 史鹏辉, 方彦, 武军艳, 曾秀存, 孔德晶, 鲁美宏, 王月 . 北方冬油菜抗寒性的形态与生理机制. 中国农业科学, 2014,47(3):452-461. doi: 10.3864/j.issn.0578-1752.2014.03.005
	YANG N N, SUN W C, LIU Z G, SHI P H, FANG Y, WU J Y, ZENG X C, KONG D J, LU M H, WANG Y . Morphological characters and physiological mechanisms of cold resistance of winter rapeseed in northern China. Scientia Agricultura Sinica, 2014,47(3):452-461. (in Chinese) doi: 10.3864/j.issn.0578-1752.2014.03.005
[15]	李瑞雪, 金晓玲, 胡希军, 柴弋霞, 蔡梦颖, 罗峰, 张方静 . 6种含笑属植物抗寒性分析与综合评价. 应用生态学报, 2017,28(5):1464-1472.
	LI R X, JIN X L, HU X J, CHAI Y X, CAI M Y, LUO F, ZHANG F J . Analysis and comprehensive evaluation on cold resistance of six varieties of Michelia.Chinese Journal of Applied Ecology, 2017,28(5):1464-1472. (in Chinese)
[16]	张佳平, 李丹青, 聂晶晶, 夏宜平 . 高温胁迫下芍药的生理生化响应和耐热性评价. 核农学报, 2016,30(9):1848-1856.
	ZHANG J P, LI D Q, NIE J J, XIA Y P . Physiological and biochemical responses to the high temperature stress and heat resistance evaluation of Paeonia lactiflora Pall. cultivars. Journal of Nuclear Agricultural Sciences, 2016,30(9):1848-1856. (in Chinese)
[17]	朱菊华, 孙星, 许斌, 梁婷, 刘明, 缪建, 赵耕毛 . 不同基因型菊芋耐盐生理及其生态适应性研究. 草业学报, 2018,27(6):120-127.
	ZHU J H, SUN X, XU B, LIANG T, LIU M, MIU J, ZHAO G M . Physiological response and ecological adaptability of different Jerusalem artichoke genotypes to salt stress. Acta Prataculturae Sinica, 2018,27(6):120-127. (in Chinese)
[18]	孙东雷, 卞能飞, 陈志德, 邢兴华, 徐泽俊, 齐玉军, 王晓军, 王幸 . 花生萌发期耐盐性综合评价及耐盐种质筛选. 植物遗传资源学报, 2017,18(6):1079-1087.
	SUN D L, BIAN N F, CHEN Z D, XING X H, XU Z J, QI Y J, WANG X J, WANG X . Comprehensive evaluation of salt tolerance and screening for salt tolerant accessions of peanut ( Arachis hypogaea L.) at germination stage. Journal of Plant Genetic Resources, 2017,18(6):1079-1087. (in Chinese)
[19]	德英, 石凤翎, 赵来喜, 穆怀彬, 解继红, 赵海霞 . 老芒麦种质资源耐盐性评价. 中国草地学报, 2017,39(6):106-111.
	DE Y, SHI F L, ZHAO L X, MU H B, XIE J H, ZHAO H X . Evaluation of the salt resistance of Elymus sibiricus germplasm resources. Chinese Journal of Grassland, 2017,39(6):106-111. (in Chinese)
[20]	李长慧, 李淑娟, 刘艳霞, 董添财, 贾科, 梁德飞 . 低温胁迫对10份鹅观草属野生种抗寒生理指标的影响. 草业科学, 2018,35(1):123-132.
	LI C H, LI S J, LIU Y X, DONG T C, JIA K, LIANG D F . Effect of low temperature stress on the cold-resistance physiological indexes of 10 germplasm resources of Roegneria in Qinghai. Pratacultural Science, 2018,35(1):123-132. (in Chinese)
[21]	陈晶晶, 王英哲, 金艳, 郭强, 徐博 . 不同紫花苜蓿杂交组合对冻害胁迫的生理响应. 草业科学, 2018,35(5):1138-1144.
	CHEN J J, WANG Y Z, JIN Y, GUO Q, XU B . Physiological responses of different alfalfa hybrid combinations to freezing stress. Pratacultural Science, 2018,35(5):1138-1144. (in Chinese)
[22]	王兰芬, 武晶, 景蕊莲, 程须珍, 王述民 . 绿豆种质资源成株期抗旱性鉴定. 作物学报, 2015,41(8):1287-1294. doi: 10.3724/SP.J.1006.2015.01287
	WANG L F, WU J, JING R L, CHENG X Z, WANG S M . Identification of Mungbean germplasm resources resistant to drought at adult stage. Acta Agronomica Sinica, 2015,41(8):1287-1294. (in Chinese) doi: 10.3724/SP.J.1006.2015.01287
[23]	于天一, 林建材, 孙学武, 冯昊, 孙秀山, 吴正锋, 郑永美, 沈浦, 王才斌 . 花生幼苗耐酸鉴定指标筛选及综合评价. 中国油料作物学报, 2017,39(4):488-495.
	YU T Y, LIN J C, SUN X S, FENG H, SUN X S, WU Z F, ZHENG Y M, SHEN P, WANG C B . Screen of acid resistance evaluation indicators and comprehensive evaluation in peanut seedlings of different genotypes. Chinese Journal of Oil Crop Sciences, 2017,39(4):488-495. (in Chinese)
[24]	熊洁, 邹小云, 陈伦林, 李书宇, 邹晓芬, 宋来强 . 油菜苗期耐铝基因型筛选和鉴定指标的研究. 中国农业科学, 2015,48(16):3112-3120. doi: 10.3864/j.issn.0578-1752.2015.16.002
	XIONG J, ZOU X Y, CHEN L L, LI S Y, ZOU X F, SONG L Q . Screening of rapeseed genotypes with aluminum tolerance at seedling stage and evaluation of selecting indices. Scientia Agricultura Sinica, 2015,48(16):3112-3120. (in Chinese) doi: 10.3864/j.issn.0578-1752.2015.16.002
[25]	陈致富, 李勤菲, 张永晶, 崔艺馨, 许汪洁, 贺亚军, 万华方, 李晓荣, 钱伟 . 白菜型油菜品种萌发期的抗旱性鉴定与筛选. 植物遗传资源学报, 2015,16(1):15-22.
	CHEN Z F, LI Q F, ZHANG Y J, CUI Y X, XU W J, HE Y J, WAN H F, LI X R, QIAN W . Identification and screening of resources with tolerance against drought stress in Brassica rapa during germination stage. Journal of Plant Genetic Resources, 2015,16(1):15-22. (in Chinese)
[26]	鲜孟筑, 杨萍, 胡立勇, 徐正华 . 油菜种子萌发成苗期耐低温性评价. 作物杂志, 2015(5):116-122.
	XIAN M Z, YANG P, HU L Y, XU Z H . Comprehensive evaluation of low temperature tolerance in rapeseed during germination and emergence periods. Crops, 2015(5):116-122. (in Chinese)
[27]	CHOUARD P . Vernalization and its relations to dormancy. Annual Review of Plant Physiology, 1960,11(1):191-238.
[28]	HAWKINS G P, DENG Z A, KUBIK T J, JOHNSON-FLANAGAN A M . Characterization of freezing tolerance and vernalization in Vern-, a spring-type Brassica napus line derived from a winter cross. Planta (Berlin), 2002,216(2):220-226.
[29]	FOWLER D B, LIMIN A E, WANG S Y, WARD R W . Relationship between low-temperature tolerance and vernalization response in wheat and rye. Canadian Journal of Plant Science, 1996,76(1):37-42.
[30]	伍晓明, 陈碧云, 陆光远 . 油菜种质资源描述规范和数据标准. 北京: 中国农业出版社, 2007.
	WU X M, CHEN B Y, LU G Y. Description and Data Standard for Rapeseed Germplasm Resources. Beijing: China Agricultural Press, 2007. (in Chinese)
[31]	邹琦 . 植物生理学实验指导. 北京: 中国农业出版社, 2000.
	ZOU Q. Plant Physiology Experiment Guide. Beijing: China Agricultural Press, 2000. (in Chinese)
[32]	崔党群 . Logistic曲线方程的解析与拟合优度测验. 数理统计与管理, 2005(1):112-115.
	CUI D Q . Analysis and making good fitting degree test for logistic curve regression equation. Mathematical Statistics and Management, 2005(1):112-115. (in Chinese)
[33]	刘自刚, 孙万仓, 杨宁宁, 王月, 何丽, 赵彩霞, 史鹏飞, 杨刚, 李学才, 武军艳, 方彦, 曾秀存 . 冬前低温胁迫下白菜型冬油菜抗寒性的形态及生理特征. 中国农业科学, 2013,46(22):4679-4687. doi: 10.3864/j.issn.0578-1752.2013.22.005
	LIU Z G, SUN W C, YANG N N, WANG Y, HE L, ZHAO C X, SHI P F, YANG G, LI X C, WU J Y, FANG Y, ZENG X C . Morphology and physiological characteristics of cultivars with different levels of cold-resistance in winter rapeseed ( Brassica campestris L.) during cold acclimation. Scientia Agricultura Sinica, 2013,46(22):4679-4687. (in Chinese) doi: 10.3864/j.issn.0578-1752.2013.22.005
[34]	王丽萍, 杨刚, 孙万仓, 张正丽, 王月, 董红业, 赵艳宁, 孔德晶, 鲁美宏, 杨宁宁, 刘自刚, 方彦, 武军艳 . 白菜型冬油菜和春油菜杂交后代抗寒性与植物学特性. 干旱地区农业研究, 2015(4):291-296.
	WANG L P, YANG G, SUN W C, ZHANG Z L, WANG Y, DONG H Y, ZHAO Y N, KONG D J, LU M H, YANG N N, LIU Z G, FANG Y, WU J Y . Cold resistance and botanical characteristics of the hybrid progeny between winter and spring turnip rapes (Brassica campestris L.). Agricultural Research in Arid Regions, 2015(4):291-296. (in Chinese)
[35]	武军艳, 方彦, 张朋飞, 杨月蓉, 孙万仓, 刘自刚, 李学才 . 北方旱寒区冬油菜根系抗寒指标分析. 干旱地区农业研究, 2014(6):250-255.
	WU J Y, FANG Y, ZHANG P F, YANG Y R, SUN W C, LIU Z G, LI X C . Analysis of cold-tolerant characters of winter rape roots in northern arid-cold area. Agricultural Research in Arid Regions, 2014(6):250-255. (in Chinese)
[36]	CHEN Y, JIANG J F, CHANG Q S, GU C S, SONG A P, CHEN S M, DONG B, CHEN F D . Cold acclimation induces freezing tolerance via antioxidative enzymes, proline metabolism and gene expression changes in two chrysanthemum species. Molecular Biology Reports, 2014,41(2):815-822.
[37]	ZHANG X Z, WANG K H, ERVIN E H, WALTZ C, MURPHY T . Metabolic changes during cold acclimation and deacclimation in ﬁve bermudagrass varieties: I. Proline, total amino acid, protein, and dehydrin expression. Crop Science, 2011,51:838-846.
[38]	苏李维, 李胜, 马绍英, 戴彩虹, 时振振, 唐斌, 赵生琴, 蒲彦涛 . 葡萄抗寒性综合评价方法的建立. 草业学报, 2015,24(3):70-79.
	SU L W, LI S, MA S Y, DAI C H, SHI Z Z, TANG B, ZHAO S Q, PU Y T . A comprehensive assessment method for cold resistance of grape vines. Acta Prataculturae Sinica, 2015,24(3):70-79. (in Chinese)
[39]	闫彪 . 甘蓝型油菜响应低温胁迫的差异蛋白质组学分析[D]. 郑州: 郑州大学, 2012: 21-30.
	YAN B . Differential proteomics analysis of Brassica napus L. in response to low temperature stress[D]. Zhengzhou: Zhengzhou University, 2012: 21-30. (in Chinese)
[40]	WAALEN W M, STAVANG J A, OLSEN J E . The relationship between vernalization saturation and the maintenance of freezing tolerance in winter rapeseed. Environmental & Experimental Botany, 2014,106(1):164-173.
[41]	孙万仓, 侯献飞, 杨刚, 刘自刚, 曾秀存, 武军艳, 方彦, 李学才, 郭仁迪 . 一种利用高温诱导鉴定白菜型冬油菜抗寒性的方法: 中国, CN104982173A, 2015 -10-21.
	SUN W C, HOU X F, YANG G, LIU Z G, ZENG X C, WU J Y, FANG Y, LI X C, GUO R D . Method for identifying cold resistance of winter rapeseed by using high temperature induction: China,CN104982173A, 2015 -10-21. (in Chinese)
[42]	ARMATRONG J J, TAKEBAYASHI N, SFORMO T, WOLF D E . Cold tolerance in Arabidopsis kamchatica. American Journal of Botany, 2015,102:439-448.
[43]	PEIXTO M M, FRIESEN P C, SAGE R F . Winter cold tolerance thresholds in field-grown Miscanthus hybrid rhizomes. Journal of Experimental Botany, 2015,66:4415-4425.
[44]	张龙龙, 杨明明, 董剑, 赵万春, 高翔, 陈冬阳 . 三个小麦新品种不同生育阶段抗旱性的综合评价. 麦类作物学报, 2016,36(4):426-434.
	ZHANG L L, YANG M M, DONG J, ZHAO W C, GAO X, CHEN D Y . Comprehensive analysis of drought resistance of three new wheat cultivars at different growth stages. Journal of Wheat Crops, 2016,36(4):426-434. (in Chinese)
[45]	罗俊杰, 欧巧明, 叶春雷, 王方, 王镛臻, 陈玉梁 . 重要胡麻栽培品种的抗旱性综合评价及指标筛选. 作物学报, 2014,40(7):1259-1273. doi: 10.3724/SP.J.1006.2014.01259
	LUO J J, OU Q M, YE C L, WANG F, WANG Y Z, CHEN Y L . Comprehensive valuation of drought resistance and screening of indices of important flax cultivars. Journal of Crops, 2014,40(7):1259-1273. (in Chinese) doi: 10.3724/SP.J.1006.2014.01259
[46]	罗耀, 席嘉宾, 谭筱弘, 张巨明 . 9种暖季型草坪草耐阴性综合评价及其指标的筛选. 草业学报, 2013,22(5):239. doi: 10.11686/cyxb20130528
	LUO Y, XI J B, TAN X H, ZHANG J M . Evaluation of shade tolerance of nine warm-season turfgrass and selection of their shade tolerant indices. Journal of Grass Industry, 2013,22(5):239. (in Chinese) doi: 10.11686/cyxb20130528
[47]	孙万仓 . 北方旱寒区冬油菜栽培技术. 北京: 中国农业出版社, 2013.
	SUN W C. Techniques of Winter Rapeseed Cultivation in Arid and Cold Areas of North China. Beijing: China Agriculture Press, 2013. (in Chinese)
[48]	孙万仓 . 北方冬油菜北移与区划. 北京: 科学出版社, 2016.
	SUN W C. Migration and Regionalization of Winter Rapeseed in North China. Beijing: Science Press, 2016. (in Chinese)
[49]	孙万仓, 曾秀存, 刘自刚, 杨刚, 方彦, 武军艳, 李学才 . 轮回选择对白菜型冬油菜抗寒性及经济性状的影响. 中国油料作物学报, 2015,37(4):443-452. doi: 10.7505/j.issn.1007-9084.2015.04.003
	SUN W C, ZENG X C, LIU Z G, YANG G, FANG Y, WU J Y, LI X C . Effects of recurrent selection on cold tolerance and economic traits of Brassica rapa. Chinese Journal of Oil Crops, 2015,37(4):443-452. (in Chinese) doi: 10.7505/j.issn.1007-9084.2015.04.003
[50]	杨晓光, 刘志娟, 陈阜 . 全球气候变暖对中国种植制度可能影响: Ⅵ. 未来气候变化对中国种植制度北界的可能影响. 中国农业科学, 2011,44(8):1562-1570.
	YANG X G, LIU Z J, CHEN F . The possible effects of global warming on cropping systems in China: Ⅵ. Possible effects of future climate change on northern limits of cropping system in China. Scientia Agricultura Sinica, 2011,44(8):1562-1570. (in Chinese)
[51]	王栓全, 刘冬梅 . 甘蓝型油菜北移的栽培技术. 作物杂志, 1995(1):20-21.
	WANG S Q, LIU D M . Cultivation techniques ofBrassica napus L. Journal of Crops, 1995(1):20-21. (in Chinese)

品种（系） Variety (line)	类型 Type	选育单位 Source
新油23 Xinyou23	甘蓝型冬油菜 Winter Brassica napus	新疆农业科学院 Xinjiang Academy of Agricultural Sciences
16TS 309-4	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
16TS 306-3	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
16TS 312-2	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
16TS 309-10	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
16NPZ 269-1	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
16NTS 158	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
15NS 45-4	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
2016 8(G)	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
2016TS(G)10	甘蓝型冬油菜 Winter Brassica napus	甘肃农业大学 Gansu Agricultural University
天油14 Tianyou14	甘蓝型冬油菜 Winter Brassica napus	天水市农业科学研究所 Tianshui Agricultural Science Research Institute
天油2288 Tianyou2288	甘蓝型冬油菜 Winter Brassica napus	天水市农业科学研究所 Tianshui Agricultural Science Research Institute

环境因子 Environment factor	天水 Tianshui	上川 Shangchuan	兰州 Lanzhou
经度 Longitude	105°69′ E	103°40′ E	103°73′ E
纬度 Latitude	34°60′ N	36°03′ N	36°73′ N
海拔 Altitude (m)	1084—1650	2150	1517
年平均气温 Annual average temperature (℃)	11.5	6.5	10.3
最冷月平均最低气温 Lowest temperature in the coldest month (℃)	-2.0	-14.6	-5.0
极端气温 Extreme low temperature (℃)	-14.7	-26.5	-16.0
无霜期 Free frost days (d)	155	142	180
平均降雨量 Precipitation (mm)	491	175	327

品种（系） Variety (line)	越冬率Overwintering rate (%)									抗寒性分类 Cold tolerance classification
	天水Tianshui			兰州Lanzhou			上川Shangchuan
	2016—2018		平均值Mean	2016—2018		平均值 Mean	2016—2018		平均值 Mean
新油23 Xinyou23	94.1	90.1	92.1a	6.0	40.0	23.0g	0.0	0.0	0.0e	弱 Weak
16TS 309-4	98.4	96.2	97.3a	21.0	65.0	43.0bc	4.9	14.3	9.6a	强 Strong
16TS 306-3	98.9	96.5	97.7a	15.4	57.5	36.5d	1.8	13.6	7.7bc	中 Medium
16TS 312-2	94.1	93.7	93.9a	22.5	65.4	44.0ab	3.3	13.7	8.5ab	强 Strong
16TS 309-10	98.8	94.0	96.4a	15.6	44.2	30.0f	3.8	12.6	8.2abc	中 Medium
16NPZ 269-1	97.7	92.5	95.1a	19.5	54.8	37.2d	2.9	11.7	7.3bcd	中 Medium
16NTS 158	95.8	91.0	93.4a	16.0	50.0	33.0e	2.0	10.6	6.3cd	中 Medium
15NS 45-4	96.6	96.4	96.5a	12.0	78.6	45.3a	2.1	14.4	8.2abc	强 Strong
2016 8(G)	95.9	93.5	94.7a	20.8	63.0	42.0c	1.2	10.2	5.7d	强 Strong
2016TS(G)10	99.3	96.3	97.8a	18.5	70.0	44.3abc	4.4	11.0	7.7bc	强 Strong
天油14 Tianyou14	97.0	95.0	96.0a	9.2	36.0	22.6g	0.0	2.0	1.0e	弱 Weak
天油2288 Tianyou2288	95.9	89.5	92.7a	7.2	38.5	22.9g	0.0	0.0	0.0e	弱 Weak

品种（系） Variety(line)	相对电导率 Relative electrolytic leakage (%)					回归方程 Regression equation	拟合度 R²	半致死温度 LT₅₀(℃)	抗寒性排序 Cold tolerance ranking
品种（系） Variety(line)	5℃	0℃	-5℃	-10℃	-15℃	回归方程 Regression equation	拟合度 R²	半致死温度 LT₅₀(℃)	抗寒性排序 Cold tolerance ranking
新油23 Xinyou23	25.30b	32.54a	42.40ab	61.27a	80.45abc	y=100/（1+1.949e^0.1237^x）	0.96	-5.4	11
16TS 309-4	20.07cd	31.20b	31.83abc	46.67a	68.67c	y=100/（1+2.6e^0.0998^x）	0.92	-9.6	2
16TS 306-3	19.87de	22.03b	32.22cd	48.80a	80.55abc	y=100/（1+2.976e^0.1369^x）	0.89	-8.0	3
16TS 312-2	17.26de	28.57b	34.20a	53.07a	85.92ab	y=100/（1+2.775e^0.1558^x）	0.90	-6.6	7
16TS 309-10	15.14bc	21.75c	36.16cd	52.03a	85.97ab	y=100/（1+3.251e^0.1687^x）	0.93	-7.0	5
16NPZ 269-1	15.95e	25.16c	34.23bcd	66.31a	88.85a	y=100/（1+2.872e^0.1849^x）	0.94	-5.7	9
16NTS 158	19.60bc	26.33b	39.00abcd	54.63a	86.36ab	y=100（1+2.57e^0.1546^x）	0.91	-6.1	8
15NS 45-4	20.05e	35.76bc	37.73abcd	57.80a	84.99ab	y=100/（1+2.496e^0.1256^x）	0.94	-7.3	4
2016 8(G)	25.92bc	31.67a	41.23ab	52.13a	75.63bc	y=100/（1+2.04e^0.1044^x）	0.93	-6.8	6
2016TS(G)10	20.66bc	21.21b	29.37d	41.37a	57.63d	y=100/（1+3.134e^0.0854^x）	0.93	-13.4	1
天油14 Tianyou14	20.71bc	29.04a	32.90abcd	58.17a	76.61bc	y=100/（1+2.22e^0.1427^x）	0.91	-5.6	10
天油2288 Tianyou2288	22.71a	34.65a	40.22ab	73.63a	79.17abc	y=100/（1+1.734e^0.125^x）	0.91	-4.4	12

品种（系） Variety(line)	心叶色 Leaf color	幼茎色 Young stem color	叶柄色 Petiole color	生长习性 Growth habit	叶片数 Leaves amount
新油23 Xinyou23	黄绿/紫色 Yellow-green/Purple	黄绿/紫色 Yellow-green/Purple	黄绿/紫色 Yellow-green/Purple	半直立/匍匐 Semi-erect/Prostrate	12.0
16TS 309-4	紫色 Purple	绿色 Green	黄绿/紫色 Yellow-green/Purple	半直立/匍匐 Semi-erect/Prostrate	9.8
16TS 306-3	紫色 Purple	黄绿 Yellow-green	黄绿/紫色 Yellow-green/Purple	半直立/匍匐 Semi-erect/Prostrate	9.1
16TS 312-2	紫色 Purple	紫色 Purple	黄绿 Yellow-green	半直立 Semi-erect	10.7
16TS 309-10	紫色 Purple	紫色 Purple	黄绿/紫色 Yellow-green/Purple	半直立/匍匐 Semi-erect/Prostrate	9.8
16NPZ 269-1	紫色 Purple	黄绿 Yellow-green	黄绿 Yellow-green	半直立/匍匐 Semi-erect/Prostrate	11.0
16NTS 158	黄绿/紫色 Yellow-green/Purple	黄绿 Yellow-green	黄绿 Yellow-green	半直立/匍匐 Semi-erect/Prostrate	11.3
15NS 45-4	紫色 Purple	黄绿 Yellow-green	黄绿/紫色 Yellow-green/Purple	半直立/匍匐 Semi-erect/Prostrate	10.0
2016 8(G)	紫色 Purple	黄绿/紫色 Yellow-green/Purple	黄绿/紫色 Yellow-green/Purple	半直立/匍匐 Semi-erect/Prostrate	9.1
2016TS(G)10	紫色 Purple	黄绿 Yellow-green	黄绿 Yellow-green	半直立/匍匐 Semi-erect/Prostrate	10.8
天油14 Tianyou14	黄绿/紫色 Yellow-green/Purple	黄绿 Yellow-green	黄绿 Yellow-green	半直立 Semi-erect	12.6
天油2288 Tianyou2288	黄绿/紫色 Yellow-green/Purple	黄绿 Yellow-green	黄绿 Yellow-green	半直立 Semi-erect	12.8

北方强冬性甘蓝型冬油菜品种(系)抗寒性评价

Comprehensive Assessment on Cold Tolerance of the Strong Winter Brassica napus L. Cultivated in Northern China

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品种（系） Variety (line)	地上部干重 Above-ground dry matter		地下部干重 Under-ground dry matter		干根冠比 Dry root shoot ratio
品种（系） Variety (line)	9/26	10/29	9/26	10/29	9/26	10/29
新油23 Xinyou23	4.75a	10.98abcd	0.73a	3.86bcd	0.16a	0.36bcd
16TS 306-4	3.40ab	5.35d	0.82a	5.12abc	0.30a	0.95abc
16TS 306-3	3.29ab	6.31bcd	0.87a	2.56cd	0.27a	0.40cd
16TS 312-2	3.91ab	14.58ab	0.79a	3.67bcd	0.21a	0.24bcd
16TS 309-10	3.26ab	5.51cd	0.73a	2.69cd	0.23a	0.49cd
16NPZ269-1	2.69b	14.12abc	0.65a	6.08ab	0.24a	0.46ab
16NTS 158	4.58ab	12.24abcd	0.66a	2.27d	0.14a	0.19d
15NS 45-4	3.32ab	8.95abcd	0.89a	7.08a	0.27a	0.88a
2016 8(G)	3.78ab	5.69cd	0.88a	2.39d	0.24a	0.42d
2016TS(G)10	3.66ab	7.52abcd	0.73a	4.21bcd	0.20a	0.59bcd
天油14 Tianyou14	2.62b	14.72ab	0.58a	3.25cd	0.22a	0.23cd
天油2288 Tianyou2288	3.91ab	15.65a	0.78a	2.25d	0.28a	0.25d

品种（系） Variety (line)	春化率 Vernalization rate (%)	小区平均株数 Amount	比例Ratio (%)				抗寒性分类 Cold tolerance classification
品种（系） Variety (line)	春化率 Vernalization rate (%)	小区平均株数 Amount	未现蕾 Not budding	现蕾期 Squaring stage	花期 Flowering stage	成熟期 Maturing stage	抗寒性分类 Cold tolerance classification
新油23 Xinyou23	87.65a	162	12.35	39.51	—	48.15	Ⅴ
16TS 309-4	9.39g	181	90.61	9.39	—	—	Ⅰ
16TS 306-3	17.16f	204	82.84	17.16	—	—	Ⅱ
16TS 312-2	27.13e	387	72.87	27.13	—	—	Ⅱ
16TS 309-10	25.00e	216	75.00	24.54	—	0.46	Ⅱ
16NPZ 269-1	43.98c	241	56.43	39.00	0.41	4.15	Ⅲ
16NTS 158	38.71d	124	61.29	38.71	—	—	Ⅲ
15NS 45-4	16.40f	189	83.60	16.40	—	—	Ⅱ
2016 8(G)	24.37e	119	75.63	23.53	0.84	—	Ⅱ
2016TS(G)10	4.05h	222	95.95	4.05	—	—	Ⅰ
天油14 Tianyou14	55.00b	100	45.00	31.00	7.00	17.00	Ⅳ
天油2288 Tianyou2288	85.19a	108	14.81	10.19	2.78	72.22	Ⅴ

品种（系） Variety (line)	平均值Mean		平均株（薹）高 Average height of plant (bolt) (cm)
品种（系） Variety (line)	春化率 Vernalization rate (%)	平均株（薹）高 Average plant (bolt) height (cm)	未现蕾 Not budding	现蕾期 Squaring stage	花期 Flowering stage	成熟期 Maturing stage
新油23 Xinyou23	87.65	80.18	28.7	44.7	—	122.5
16TS 309-4	9.39	15.88	14.5	29.2	—	—
16TS 306-3	17.16	18.74	17.8	23.3	—	—
16TS 312-2	27.13	27.46	24.8	34.6	—	—
16TS 309-10	25.00	28.84	26.2	35.5	—	103.0
16NPZ 269-1	43.98	37.83	34.5	35.6	85.0	99.3
16NTS 158	38.71	33.42	32.3	35.2	—	—
15NS 45-4	16.4	20.73	16.6	41.8	—	—
2016 8(G)	24.37	20.21	17.6	28.1	34.0	—
2016TS(G)10	4.05	10.77	10.5	17.1	—	—
天油14 Tianyou14	55.00	38.79	23.1	28.7	49.4	94.4
天油2288 Tianyou2288	85.19	58.21	22.7	33.8	46.0	69.4

指标 Index	小区平均株高 Average plant height	未现蕾 Not budding		现蕾期 Squaring stage		成熟期 Maturing stage
指标 Index	小区平均株高 Average plant height	比例 Proportion	平均薹高 Average blot height	比例 Proportion	平均薹高 Average blot height	比例 Proportion	平均株高 Plant height
春化率 Vernalization rate	0.96**	-1.00**	0.57	0.46	0.53	0.89**	0.71**

品种（系） Variety(line)	耐寒系数（K） Anti-cold coefficiency
品种（系） Variety(line)	SOD	CAT	POD	SP	SS	Pro
新油23 Xinyou23	1.267b	1.267d	1.563c	1.478a	2.870ab	2.292c
16TS 309-4	1.377b	0.688d	3.369bc	1.316abcd	3.898a	3.633c
16TS 306-3	1.459b	0.486cd	2.765abc	1.278bcd	3.248ab	2.959bc
16TS 312-2	1.259ab	0.252bcd	1.844abc	1.174cd	2.889ab	2.356bc
16TS 309-10	1.629ab	0.407bcd	2.045abc	1.336abc	3.229ab	2.167bc
16NPZ 269-1	1.393ab	0.232bcd	2.064abc	1.243bcd	2.747ab	1.995bc
16NTS 158	1.291ab	0.184bcd	1.950abc	1.090d	2.794ab	2.573bc
15NS 45-4	1.242ab	0.155abc	2.846abc	1.395abc	3.267ab	2.307bc
2016 8(G)	1.426ab	0.158abc	2.818abc	1.243bcd	2.829ab	2.293ab
2016TS(G)10	1.565ab	0.157abc	2.641abc	1.426ab	3.325ab	2.318ab
天油14 Tianyou14	1.375ab	0.125ab	2.157ab	1.267bcd	2.688ab	2.007a
天油2288 Tianyou2288	1.198a	0.100a	1.542a	1.100d	2.338b	2.990a

品种（系） Variety (line)	隶属函数值 Subordinative function						综合评价值(D) Comprehensive evaluation value	抗寒性排序 Cold tolerance ranking
品种（系） Variety (line)	μ（1）	μ（2）	μ（3）	μ（4）	μ（5）	μ（6）	综合评价值(D) Comprehensive evaluation value	抗寒性排序 Cold tolerance ranking
新油23 Xinyou23	0.000	0.436	0.000	0.125	0.643	0.000	0.343	12
16TS 309-4	4.674	0.820	0.828	0.591	0.869	0.944	2.961	2
16TS 306-3	3.609	0.705	0.002	0.020	0.899	1.000	2.378	3
16TS 312-2	2.804	0.352	0.194	0.355	0.777	0.740	1.839	7
16TS 309-10	3.935	0.454	0.299	0.101	1.000	0.680	2.364	4
16NPZ 269-1	1.652	0.190	0.051	0.183	0.942	0.373	1.180	9
16NTS 158	2.304	0.271	1.000	0.754	0.409	0.540	1.544	8
15NS 45-4	3.413	1.000	0.935	1.000	0.122	0.949	2.345	5
2016 8(G)	3.022	0.520	0.528	0.138	0.918	0.853	2.090	6
2016TS(G)10	5.283	0.754	0.359	0.692	0.744	0.940	3.106	1
天油14 Tianyou14	1.674	0.242	0.302	0.583	0.460	0.401	1.143	10
天油2288 Tianyou2288	1.000	0.000	0.105	0.000	0.000	0.179	0.493	11
权重系数 Weighted value	0.249	0.209	0.068	0.051	0.174	0.248

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