苯磺隆胁迫下甘蓝型油菜萌发期关联性状的QTL定位及候选基因筛选

doi:10.3864/j.issn.0578-1752.2020.08.002

摘要/Abstract

摘要：

【目的】寻找苯磺隆胁迫下油菜种子萌发性状相关的QTL及其耐性基因,为筛选与培育耐苯磺隆油菜种质以及探究油菜种子萌发过程中苯磺隆耐性分子机理奠定基础。【方法】用0.15 mg·kg ^-1苯磺隆溶液处理由人工合成甘蓝型油菜10D130和甘蓝型油菜常规品种ZS11构建的包含175个株系的高世代重组自交系（RIL）群体,进行种子发芽试验,以蒸馏水为对照,分别测定其相对发芽势、相对发芽率、相对根长和相对干重。然后,利用油菜6K SNP芯片对该RIL群体进行基因分型,通过JoinMap4.0软件构建高密度遗传连锁图谱。基于该遗传图谱,利用MapQTL软件多QTL作图法对4个性状的相对值进行QTL定位,根据各QTL置信区间查找甘蓝型油菜的基因序列,并依次与拟南芥基因组序列进行BLAST,筛选可能与耐苯磺隆胁迫相关的候选基因。【结果】频数分布表明4个相对性状的变异范围较大,且呈连续性分布,符合数量性状表现特征,适宜进行QTL遗传分析。相关分析表明,相对发芽率和相对发芽势呈极显著正相关,相关系数为0.587。构建的遗传图谱包含1 897个多态性SNP标记,覆盖甘蓝型油菜基因组3 214.19 cM,标记之间的平均图距为1.69 cM。利用此图谱共检测到22个相关QTL,表型贡献率变幅为6.4%—12.6%。其中,与相对发芽势、相对发芽率相关的QTL分别有6个和3个,与相对根长和相对干重有关的QTL分别为8个和5个。在A01染色体64.857 cM、55.935 cM和56.645 cM处检测到的相对发芽势与相对发芽率QTL的置信区间完全或者部分重叠。通过分析QTL置信区间上甘蓝型油菜对应的区间序列,筛选到30个可能与油菜耐苯磺隆有关的候选基因,其中包括18个细胞色素P450家族成员、5个糖基转移酶家族基因、1个GSTF相关基因、1个ABC转运蛋白相关基因和1个ALS基因,这些基因均与除草剂抗性机制有关,尤其ALS为磺酰脲类除草剂靶位点酶;另外筛选到1个BHLH和1个JAZ6基因,BHLH与JAZ蛋白可通过相互作用来防御胁迫;检测到1个LSU2蛋白相关基因和1个MATE家族成员,前者参与细胞氧化剂解毒及植物防御反应,后者参与类黄酮、生物碱、金属离子、其他多种代谢物的转运及有毒物质引起的植物胁迫响应。【结论】检测到与相关QTL共22个,筛选出可能与苯磺隆耐性有关的候选基因30个。这些基因通过加速毒性分子的转运与代谢从而响应有毒物质引起的胁迫反应,可能参与植物对苯磺隆的抗性调节与反应机制。

关键词: 苯磺隆, 甘蓝型油菜, 萌发期, QTL, 候选基因

Abstract:

【Objective】The QTLs and tolerance genes related to the germination characters of rape seeds under the stress of tribenuron-methyl were studied, which laid the foundation for screening and cultivating the germplasm of tribenuron-methyl resistant rape and exploring the molecular mechanism of tribenuron-methyl tolerance during the germination of rape seeds.【Method】A high generation RIL population consisted of 175 lines, which were constructed from the synthetic Brassica napus 10D130 and the conventional variety Brassica napus ZS11, was treated with 0.15 mg·kg ^-1 tribenuron-methyl solution for seed germination test and the control was under the distilled water. The phenotypic data that including relative germination vigor (RGV), relative germination rate (RGR), relative root length (RRL) and relative dry weight (RDW) were analyzed by Excel software. Then, the RIL population was genotyped with 6K SNP chip, and the high-density genetic linkage map was constructed by JoinMap4.0 software. Based on the genetic map, the relative values of four characters were mapped by using Multiple QTL mapping method of MapQTL software. And the genes sequence of Brassica napus were searched according to the confidence interval of each QTL, next, blast with Arabidopsis genome sequence in turn and select the candidate genes that may be related to the tolerance to tribenuron-methyl stress.【Result】The frequency distribution of each traits for RIL population's was continuous with the large variation range, which were consistent with the characteristics of quantitative characters, so it were suitable for the detection of QTL. Correlation analysis showed that there was a significant positive correlation between RGR and RGV, and the correlation coefficient was 0.587. In addition, the constructed genetic map contained 1 897 polymorphic SNP markers covering 3 214.19 cM of the genome of Brassica napus with an average map distance of 1.69 cM. By this map, 22 QTLs related to 4 phenotypic traits were detected and the phenotypic contribution rate was between 6.4% and 12.6%. Among them, there were 6 and 3 QTLs related to RGV and RGR, 8 and 5 QTLs related to RRL and RDW, respectively. Also, the confidence intervals of QTLs for RGV and RGR were found to overlap completely or partially at 64.857 cM, 55.935 cM and 56.645 cM of chromosome A01. Through sequence alignment, 30 candidate genes were screened, including 18 cytochrome P450 family members, 5 glycosyltransferase family genes, 1 GSTF related gene, 1 ABC transporter related gene and 1 ALS gene, all of which were detoxified by accelerating metabolism, and related to the mechanism of herbicide resistance, especially ALS is the target enzyme of sulfonylurea herbicide. Furthermore, others genes were screened, including 1 BHLH gene and 1 JAZ6 gene which could interact to protect against stress; and 1 LSU2 protein gene which was involved in the detoxification of cell oxidants and plant defense response; and 1 MATE family member which was involved in the transport of flavonoids, alkaloids, metal ions, other metabolites and plant stress response caused by toxic substances.【Conclusion】22 QTLs that significantly associated with tribenuron-methyl tolerance related traits and 30 candidate genes for the tolerance to tribenuron-methyl were found. These genes are involved in the stress response caused by toxic substances by accelerating the transport and metabolism of toxic molecules, which may be related to the resistance regulation and response mechanism of plants to tribenuron-methyl.

Key words: tribenuron-methyl, Brassica napus L., germination, QTL, candidate genes

王刘艳,王瑞莉,叶桑,郜欢欢,雷维,陈柳依,吴家怡,孟丽姣,袁芳,唐章林,李加纳,周清元,崔翠. 苯磺隆胁迫下甘蓝型油菜萌发期关联性状的QTL定位及候选基因筛选[J]. 中国农业科学, 2020, 53(8): 1510-1523.

WANG LiuYan,WANG RuiLi,YE Sang,GAO HuanHuan,LEI Wei,CHEN LiuYi,WU JiaYi,MENG LiJiao,YUAN Fang,TANG ZhangLin,LI JiaNa,ZHOU QingYuan,CUI Cui. QTL Mapping and Candidate Genes Screening of Related Traits in Brassica napus L. During the Germination Under Tribenuron-Methyl Stress[J]. Scientia Agricultura Sinica, 2020, 53(8): 1510-1523.

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苯磺隆耐性相关性状的候选基因"

性状 Trait	位点 Loci	染色体 Chr.	物理区间 Physical interval (bp)	候选基因 Candidate gene	拟南芥基因 Arabidopsis gene	基因 Gene	描述 Description
相对发芽势 RGV	qRGVC07-1	C07	33957781—34814007	BnaC07g29830D	AT5G24660	LSU2	细胞氧化剂解毒;对防御反应调控 Cellular oxidant detoxification; Regulation of defense response
相对发芽势 RGV	qRGVA01-1	A01	6196365—6753945	BnaA01g12870D	AT4G23030	/	MATE家族蛋白 MATE efflux family protein
相对发芽率 RGR	qRGRA01-1	A01	6196365—6753945	BnaA01g12870D	AT4G23030	/	MATE家族蛋白 MATE efflux family protein
相对发芽率 RGR	qRGRA03-1	A03	14987907—15498700	BnaA03g31340D	AT3G10670	ABCI6	参与生物发生或修复氧化破坏的Fe-S簇 Involved in the biogenesis or repair of oxidatively damaged Fe-S clusters.
相对根长 RRL	qRRLA04-1	A04	14446411—15117001	BnaA04g19030D	AT2G32740	GT13	半乳糖基转移酶13 Galactosyltransferase 13
				BnaA04g17910D	AT2G30860	GSTF9	编码GST phi类谷胱甘肽转移酶 Encodes glutathione transferase belonging to the phi class of GSTs
				BnaA04g18440D	AT2G31790	UGT	UDP-糖基转移酶超家族蛋白 UDP-Glycosyltransferase superfamily protein
	qRRLA04-2	A04	15913314—16484458	BnaA04g21090D	AT2G36760	UGT73C2	UDP-葡萄糖基转移酶73C2 UDP-glucosyl transferase 73C2
	qRRLA04-3	A04	17814614—17923085	BnaA04g24370D	AT2G42250	CYP712A1	CYP712A的成员 Member of CYP712A
	qRRLA06-1	A06	21396095—21528844	BnaA06g31960D	AT4G39510	CYP96A12	CYP96A的成员 Member of CYP96A
				BnaA06g32050D	AT4G27710	CYP709B3	CYP709B的成员 Member of CYP709B
	qRRLA06-2	A06	21528844—22092492	BnaA06g32370D	AT3G25180	CYP82G1	编码细胞色素P450单加氧酶 Encodes a cytochrome P450 monooxygenase
				BnaA06g32970D	AT3G26290	CYP71B26	细胞色素P450成员 Putative cytochrome P450
				BnaA06g32980D	AT2G02580	CYP71B9	CYP71B的成员 Member of CYP71B
				BnaA06g32990D	AT3G26300	CYP71B34	细胞色P450 Putative cytochrome P450
				BnaA06g33000D	AT3G48560	ALS	催化乙酰乳酸的形成;被磺酰脲类除草剂抑制等 Catalyzing the formation of acetolactate; Inhibited by the sulphonylurea herbicide. etc
				BnaA06g33010D	AT3G26220	CYP71B3	细胞色素P450单加氧酶 Cytochrome P450 monooxygenase
				BnaA06g33020D	AT3G26210	CYP71B23	细胞色素P450成员 Putative cytochrome P450
				BnaA06g33030D	AT3G26200	CYP71B22	细胞色素P450成员 Putative cytochrome P450
				BnaA06g33040D	AT3G26190	CYP71B21	细胞色素P450成员 Putative cytochrome P450
				BnaA06g33060D	AT3G26170	CYP71B19	细胞色素P450成员 Putative cytochrome P450
				BnaA06g33050D	AT3G26180	CYP71B20	细胞色素P450成员 Putative cytochrome P450
				BnaA06g33070D	AT3G26165	CYP71B18	细胞色素P450成员 Putative cytochrome P450.
	qRRLC01-1	C01	3631449—3936101	BnaC01g06930D	AT1G13080	CYP71B2	细胞色素P450单加氧酶 Cytochrome P450 monooxygenase
相对干重 RDW	qRDWA02-1	A02	8524524—9460659	BnaA02g15990D	AT1G72450	JAZ6	调节防御反应及茉莉酸介导的信号传导途径Regulation of defense response and jasmonic acid mediated signaling pathway
相对干重 RDW				BnaA03g22720D	AT1G72210	BHLH96	BHLH DNA结合超家族蛋白 BHLH DNA-binding superfamily protein
				BnaA02g15580D	AT2G46960	CYP709B1	CYP709B的成员 Member of CYP709B
	qRDWA03-2	A03	10805287—12106754	BnaA03g22720D	AT2G26480	UGT76D1	UDP-葡萄糖基转移酶76D1 UDP-glucosyl transferase 76D1
				BnaA03g25050D	AT4G12320	CYP706A6	CYP706A的成员 Member of CYP706A
	qRDWA04-1	A04	1637957—2461363	BnaA04g03050D	AT3G57220	GT	糖基转移酶家族蛋白 Glycosyl transferase family 4 protein
	qRDWC03-1	C03	47920601—48510329	BnaC03g59160D	AT2G25160	CYP82F1	细胞色素P450成员 Cytochrome P450, family 82, subfamily F,

表6

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浓度Concentration (mg·kg^-1)	ZS11			10D130
浓度Concentration (mg·kg^-1)	均值 Mean (cm)	显著性检验 Significant (P<0.05)	降幅 Percentage reduction (%)	均值 Mean (cm)	显著性检验 Significant (P<0.05)	降幅 Percentage reduction (%)
CK	7.88	a	—	6.78	a	—
0.075	7.08	a	-10.15	5.71	a	-15.78
0.15	6.91	a	-12.31	4.16	b	-38.64
0.25	4.25	b	-46.07	2.12	c	-68.73
0.50	2.77	c	-64.85	0.86	c	-87.32

性状 Trait	亲本 Parent		F_2:7群体 F_2:7 populations
性状 Trait	ZS11	10D130	平均数±标准差Mean±SD	极差Range	变异系数CV (%)
相对发芽势RGV	1.00	0.97	0.98±0.18	1.33	18.7
相对发芽率RGR	1.00	0.97	1.00±0.14	1.04	13.5
相对根长RRL	0.88	0.61	0.53±0.19	0.92	36.0
相对干重RDW	0.99	0.87	0.98±0.15	1.89	15.1

性状 Trait	重复间差异性 Inter repetition difference			株系间差异性 Difference among strains
性状 Trait	自由度df	F	显著性Significant	自由度df	F	显著性Significant
相对发芽势RGV	2	0.597	0.551	174	3.356	3.8E^-22
相对发芽率RGR	2	1.136	0.322	174	3.707	1.2E^-25
相对根长RRL	2	0.009	0.991	174	36.920	2.5E^-157
相对干重RDW	2	0.766	0.466	174	3.175	2.5E^-20

性状 Trait	相对发芽势 RGV	相对发芽率 RGR	相对根长 RRL	相对干重 RDW
相对发芽势RGV	1
相对发芽率RGR	0.587**	1
相对根长RRL	-0.049	-0.110	1
相对干重RDW	-0.122	0.072	-0.107	1

性状 Trait	位点 Loci	左标记 Left marker	右标记 Right marker	染色体 Chr.	位置 Position (cM)	阈值 LOD	贡献率 Expl. (%)	置信区间 Confidence interval (cM)
相对发芽势 RGV	qRGVC02-1	/	AX-86224097	C02	0.000	3.04	7.7	0.000—0.468
	qRGVC03-1	AX-95665263	AX-177835191	C03	68.724	2.88	7.3	67.838—69.724
	qRGVC07-1	AX-177830550	AX-95503939	C07	79.903	2.82	7.1	72.776—80.784
	qRGVA01-1	AX-95656842	AX-182125504	A01	55.935	2.61	6.6	54.426—56.645
	qRGVA01-2	AX-182153395	AX-182155384	A01	64.857	2.65	6.7	62.861—67.428
	qRGVA06-1	AX-95501842	AX-177829720	A06	106.205	2.62	6.7	105.840—108.202
相对发芽率 RGR	qRGRA01-1	AX-95509232	AX-177830916	A01	56.645	4.31	10.7	55.935—57.420
	qRGRA01-2	AX-182153395	AX-182155384	A01	64.857	2.95	7.5	62.861—67.428
	qRGRA03-1	AX-95508795	AX-95509572	A03	127.616	2.82	7.2	125.682—129.203
相对根长 RRL	qRRLA04-1	AX-182176371	AX-95505772	A04	94.698	4.51	11.2	88.608—97.938
	qRRLA04-2	AX-95507645	AX-177833715	A04	113.308	5.12	12.6	108.264—114.008
	qRRLA04-3	AX-177827854	AX-95503983	A04	132.285	2.89	7.3	130.443—134.464
	qRRLA06-1	AX-95664740	AX-95506250	A06	98.667	3.46	8.7	97.389—101.050
	qRRLA06-2	AX-105306485	AX-95664864	A06	113.134	3.35	8.4	112.441—113.682
	qRRLA06-3	AX-95656835	AX-105307005	A06	124.073	4.01	10.0	123.077—130.636
	qRRLA09-1	AX-105307894	AX-177910950	A09	284.516	3.06	7.7	283.174—285.872
	qRRLC01-1	AX-105335237	AX-105335655	C01	45.715	2.51	6.4	42.779—47.043
相对干重 RDW	qRDWA03-1	AX-95663919	AX-95638137	A03	98.052	2.98	7.5	97.681—99.234
	qRDWA04-1	AX-95662645	AX-182137198	A04	17.105	3.2	8.1	12.178—19.860
	qRDWA04-2	AX-179307304	AX-95664963	A04	39.299	2.93	7.4	38.667—41.328
	qRDWA02-1	AX-179307763	AX-177832049	A02	125.116	2.65	6.7	121.653—129.830
	qRDWC03-1	AX-105338724	AX-105308542	C03	187.649	2.52	6.4	185.306—188.940