中国农业科学 ›› 2007, Vol. 40 ›› Issue (11): 2395-2402 .

• 作物遗传育种·种质资源 • 上一篇    下一篇

水稻铵转运蛋白基因OsAMT1;4 和OsAMT5的特征分析、功能和表达

邓若磊,谷俊涛,路文静,徐海荣,曹云飞,肖 凯   

  1. 河北农业大学农学院
  • 收稿日期:2006-10-09 修回日期:2007-01-11 出版日期:2007-11-10 发布日期:2007-11-10
  • 通讯作者: 肖凯

Characterization, Function and Expression Analysis of Ammonium TransporterGene OsAMT1;4 and OsAMT5 in Rice (Oryza sativa)

  

  1. 河北农业大学农学院
  • Received:2006-10-09 Revised:2007-01-11 Online:2007-11-10 Published:2007-11-10

摘要: 【目的】阐明水稻铵转运蛋白基因OsAMT1;4 和OsAMT5的编码蛋白特征、功能和表达。【方法】采用生物信息学技术,揭示目的基因的编码蛋白特征;通过铵离子吸收缺陷的酵母突变体功能互补技术,鉴定上述基因编码蛋白的功能;采用RT-PCR技术,研究基因的表达特征。【结果】OsAMT1;4和OsAMT5的编码阅读框为1 497 bp和1 377 bp,分别编码498和458个氨基酸残基。OsAMT1;4和OsAMT5均具有11个跨膜域,分别归属与AMT1和AMT2亚家族。OsAMT1;4和OsAMT5均具有使NH4+吸收缺陷的酵母突变体重新恢复吸收NH4+的能力。OsAMT5在叶中特异表达,随NH4+浓度增加表达增强;在不同NH4+浓度和时间处理中,根、叶均未检测到OsAMT1;4的转录本。【结论】OsAMT1;4和OsAMT5在水稻中具有吸收或转运NH4+的功能。OsAMT5为叶特异表达,OsAMT1;4的表达受到NH4+因子外其他因素的调节。

关键词: 铵转运蛋白, OsAMT1, OsAMT5, 异源互补, 基因功能, 基因表达

Abstract: 【Objective】To elucidade the characterization, function and expression pattern of two ammonium transporter genes OsAMT1;4 and OsAMT5 in rice (Oryza sativa). 【Method】The characterization of the encoded proteins by OsAMT1;4 and OsAMT5 was identified based on bioinformatics technology. The complementary analysis of function on NH4+ uptake for OsAMT1;4 and OsAMT5 was performed by genetic transformation of the yeast mutant lacking the ability of transporting NH4+. The gene expression patterns of OsAMT1;4 and OsAMT5 were analyzed by RT-PCR. 【Results】OsAMT1;4 and OsAMT5 had the open reading frame (ORF) 1497 bp and 1377 bp, encoded 498 amino acids and 458 amino acids, respectively. OsAMT1;4 and OsAMT5 belonged to AMT1 and AMT2 sub-family, all containing 11 conserved transmambrane domains, and could recovery the mutant lacking the ability of transporting NH4+ to re-uptake NH4+ in the growth medium, The transcripts of OsAMT5 were only in leaves and the expression levels were increase with the increase of NH4+ concentration in the medium, In all treatments, the transcripts of OsAMT1;4 in roots and leaves were all not detected.【Conclusion】OsAMT1;4 and OsAMT5 are involved in the uptake or transportation of NH4+ in the plants of rice. OsAMT5 is leaf specific in expression under NH4+ treatments. The expression of OsAMT1;4 is possibly regulated by other environmental conditions, rather than by NH4+.

Key words: Ammonium transporter, OsAMT1.4, OsAMT5, Gene function, Gene expression