施用生物炭和秸秆还田对农田温室气体排放及增温潜势的影响

doi:10.3864/j.issn.0578-1752.2024.05.009

摘要/Abstract

摘要：

【目的】农业生态系统增温潜势是影响全球气候变化的重要部分。本研究通过田间试验明确施用生物炭和秸秆还田对农田增温潜势的影响，以期为减缓气候变化和农业废弃物资源化利用提供理论依据。【方法】在山东省桓台县农业生态系统试验站的冬小麦-夏玉米轮作农田中开展了3年田间定位试验，试验共设置4个处理：① 对照（CK）；② 施用生物炭9.0 t·hm^-2·a^-1（C）；③ 全量秸秆还田（S）；④秸秆还田配施9.0 t·hm^-2·a^-1生物炭（CS）。4个处理均施等量的氮磷钾化肥，其中氮肥为尿素，用量为200 kg·hm^-2·a^-1，磷肥为过磷酸钙，用量为55 kg·hm^-2·a^-1，钾肥为硫酸钾，用量为40 kg·hm^-2·a^-1。采用静态暗箱-气相色谱法测定各处理温室气体（CO₂、N₂O和CH₄）的排放通量并计算净全球增温潜势（NGWP）和温室气体排放强度（GHGI），分析连续施用生物炭和秸秆还田对农田温室气体排放及增温潜势的影响。【结果】（1）综合3年的温室气体排放情况，与CK处理相比，S和CS处理的年平均生态呼吸排放量（Re）分别增加了47.8%和67.9%（P<0.05）；C处理的年平均N₂O累积排放量降低了20.3%（P<0.05），而S和CS处理的N₂O年平均累积排放量分别增加了23.6%和41.4%（P<0.05）。（2）与CK处理相比，C、S和CS处理的土壤有机碳年平均变化量（ΔSOC）均有显著增加，其中CS处理增幅最大，增加了150.6%（P<0.05）。与第1年相比，C、S和CS处理第3年的ΔSOC均有显著增加（P<0.05），分别增加了21.7%、20.8%和17.8%。各处理的NGWP和GHGI均存在显著差异，与CK相比，C、S和CS处理的年平均NGWP分别降低了163.5%、171.7%和273.0%（P<0.05），与第1年相比，C、S和CS处理第3年的NGWP均有显著降低（P<0.05），分别降低了73.4%、58.8%和54.7%。与CK相比，C、S和CS处理的年平均GHGI分别降低了236.2%、253.3%和388.9%（P<0.05），C、S和CS处理第3年的GHGI较第1年分别降低了126.3%、98.2%和108.6%（P<0.05）。就产量而言，C、S和CS处理的作物产量保持相对稳定，有轻微增长，但与CK相比无显著差异。【结论】与单施化肥相比，在施化肥的基础上添加生物炭、秸秆还田、秸秆还田配施生物炭的措施均能够在不影响作物产量的前提下抑制增温潜势，其中秸秆还田配施生物炭能够最大程度地降低农田增温潜势，因此秸秆还田配施生物炭是增加农田固碳、缓解气候变化的一项有效措施。

关键词: 生物炭, 秸秆还田, 温室气体, 冬小麦-夏玉米轮作, 土壤有机碳变化量, 净全球增温潜势, 温室气体排放强度

Abstract:

【Objective】The global warming potential of agro-ecosystem is an important part of affecting global climate change. This study clarified the effects of biochar and straw application on the global warming potential of farmland through field experiments, in order to provide theoretical basis for mitigating climate change and agricultural waste resource utilization. 【Method】In our study, a three-year field positioning experiment was carried out in the winter wheat-summer maize rotation farmland in the Agro-ecosystem Experimental Station in Huantai County, Shandong Province. Four treatments were set up in the experiment: ① control (CK); ② biochar (C); ③ straw return(S); ④ straw return combined with biochar (CS). The nitrogen, phosphorus and potassium fertilizers were applied in all treatments. The nitrogen fertilizer was 200 kg·hm^-2·a^-1 urea, the phosphate fertilizer was 55 kg·hm^-2·a^-1superphosphate and the potassium fertilizer was 40 kg·hm^-2·a^-1 potassium sulfate. We measured the flux of greenhouse gases (CO₂, N₂O and CH₄) by static chamber-gas chromatography method, calculated the net global warming potential (NGWP) and greenhouse gas emission intensity (GHGI), and analyzed the effect of biochar and straw on the greenhouse gas emissions and the net global warming potential. 【Result】(1) From the emission of greenhouse gases in the three-year experiment, the average annual cumulative Re emissions of S and CS treatments increased by 47.8% and 67.9% (P<0.05), respectively, compared with CK. The average annual cumulative N₂O emission under C treatment reduced by 20.3% than CK treatment (P<0.05), and the cumulative N₂O emission under S and CS treatments increased by 23.6% and 41.4% (P<0.05), respectively. (2) In comparison with CK treatment, soil organic carbon change (ΔSOC) of C, S and CS treatments increased significantly, and the largest increase was in the CS treatment by 150.6% (P<0.05). Compared with the first year, soil organic carbon change (ΔSOC) of C, S and CS treatments increased by 21.7%, 20.8% and 17.8% (P<0.05), respectively. There were significant differences in net global warming potential (NGWP) and greenhouse gas emission intensity (GHGI) among all treatments. Compared with CK treatment, the average annual NGWP of C, S and CS treatments decreased by 163.5%, 171.7% and 273.0% (P<0.05). Compared with the first year, the NGWP of C, S and CS treatments in the third year decreased by 73.4%, 58.8% and 54.7% (P<0.05), respectively. The annual average GHGI of C, S and CS treatments decreased by 236.2%, 253.3% and 388.9% than CK treatment (P<0.05), respectively. In comparison with the first rotation, the GHGI of C, S and CS treatments in the third rotation decreased significantly by 126.3%, 98.2% and 108.6% (P<0.05), respectively. The yield of C, S and CS treatments remained stable with a slight increase, but there was no significant difference between the yield of CK, C, S and CS treatments. 【Conclusion】Compared with applying chemical fertilizer alone, the methods of adding biochar, straw return and straw return combined with biochar could prevent the NGWP without reducing crop yield. Among these treatments, straw return combined with biochar could reduce the net global warming potential to the greatest extent. Therefore, straw return combined with biochar is an effective measure to enhance carbon sequestration and mitigate climate change.

Key words: biochar, straw return, greenhouse gas, winter wheat-summer maize rotation, soil organic carbon, net global warming potential (NGWP), greenhouse gas emission intensity (GHGI)

高尚洁, 刘杏认, 李迎春, 柳晓婉. 施用生物炭和秸秆还田对农田温室气体排放及增温潜势的影响[J]. 中国农业科学, 2024, 57(5): 935-949.

GAO ShangJie, LIU XingRen, LI YingChun, LIU XiaoWan. Effects of Biochar and Straw Return on Greenhouse Gas Emissions and Global Warming Potential in the Farmland[J]. Scientia Agricultura Sinica, 2024, 57(5): 935-949.

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参考文献 63

[1]	程琨, 潘根兴. 农业与碳中和. 科学, 2021, 73(6): 8-12.
	CHENG K, PAN G X. Agriculture and carbon neutrality. Science, 2021, 73(6): 8-12. (in Chinese) doi: 10.1126/science.73.1879.8
[2]	TAO F L, PALOSUO T, VALKAMA E, MÄKIPÄÄ R. Cropland soils in China have a large potential for carbon sequestration based on literature survey. Soil and Tillage Research, 2019, 186: 70-78. doi: 10.1016/j.still.2018.10.009
[3]	王小彬, 武雪萍, 赵全胜, 邓祥征, 蔡典雄. 中国农业土地利用管理对土壤固碳减排潜力的影响. 中国农业科学, 2011, 44(11): 2284-2293. doi: 10.3864/j.issn.0578-1752.2011.11.010.
	WANG X B, WU X P, ZHAO Q S, DENG X Z, CAI D X. Effects of cropland-use management on potentials of soil carbon sequestration and carbon emission mitigation in China. Scientia Agricultura Sinica, 2011, 44(11): 2284-2293. doi: 10.3864/j.issn.0578-1752.2011.11.010. (in Chinese)
[4]	阮俊梅, 宋振伟, 王全辉, 王利. 中国农田减缓气候变化的潜力与技术途径. 中国农学通报, 2020, 36(5): 98-102. doi: 10.11924/j.issn.1000-6850.casb20190800585
	RUAN J M, SONG Z W, WANG Q H, WANG L. The potential and technological approaches of China’s farmland to mitigate climate change. Chinese Agricultural Science Bulletin, 2020, 36(5): 98-102. (in Chinese) doi: 10.11924/j.issn.1000-6850.casb20190800585
[5]	SEMIDA W M, BEHEIRY H R, SÉTAMOU M, SIMPSON C R, ABD EL-MAGEED T A, RADY M M, NELSON S D. Biochar implications for sustainable agriculture and environment: A review. South African Journal of Botany, 2019, 127: 333-347. doi: 10.1016/j.sajb.2019.11.015
[6]	ELBASIOUNY H, ELBEHIRY F, EL-RAMADY H, HASANUZZAMAN M. Contradictory results of soil greenhouse gas emissions as affected by biochar application: special focus on alkaline soils. International Journal of Environmental Research, 2021, 15(5): 903-920. doi: 10.1007/s41742-021-00358-6
[7]	贾小玉, 闫伟明, 上官周平. 生物炭对农田土壤温室气体排放强度的调控机理研究进展. 陆地生态系统与保护学报, 2022(2): 62-73.
	JIA X Y, YAN W M, SHANGGUAN Z P. Research progress on the regulation mechanism of biochar on soil greenhouse gases emission intensity in farmland. Terrestrial Ecosystem and Conservation, 2022(2): 62-73. (in Chinese)
[8]	MUKHERJEE A, LAL R. Biochar impacts on soil physical properties and greenhouse gas emissions. Agronomy, 2013, 3(2): 313-339. doi: 10.3390/agronomy3020313
[9]	TAN G C, WANG H Y, XU N, JUNAID M, LIU H B, ZHAI L M. Effects of biochar application with fertilizer on soil microbial biomass and greenhouse gas emissions in a peanut cropping system. Environmental Technology, 2021, 42(1): 9-19. doi: 10.1080/09593330.2019.1620344
[10]	NTACYABUKURA T, UWIRINGIYIMANA E, ZHOU M H, ZHANG B W, ZHU B, HARERIMANA B, DE DIEU NAMBAJIMANA J, NSABIMANA G, NSENGUMUREMYI P. Effect of biochar and straw application on nitrous oxide and methane emissions from eutric regosols with different pH in Sichuan Basin: A mesocosm study. Atmosphere, 2021, 12(6): 729. doi: 10.3390/atmos12060729
[11]	ZHOU M R, YING S S, CHEN J H, JIANG P K, TENG Y X. Effects of biochar-based fertilizer on nitrogen use efficiency and nitrogen losses via leaching and ammonia volatilization from an open vegetable field. Environmental Science and Pollution Research, 2021, 28(46): 65188-65199. doi: 10.1007/s11356-021-15210-9
[12]	FIDEL R, LAIRD D, PARKIN T. Effect of biochar on soil greenhouse gas emissions at the laboratory and field scales. Soil Systems, 2019, 3(1): 8. doi: 10.3390/soilsystems3010008
[13]	LEHTINEN T, SCHLATTER N, BAUMGARTEN A, BECHINI L, KRÜGER J, GRIGNANI C, ZAVATTARO L, COSTAMAGNA C, SPIEGEL H. Effect of crop residue incorporation on soil organic carbon and greenhouse gas emissions in European agricultural soils. Soil Use and Management, 2014, 30(4): 524-538. doi: 10.1111/sum.2014.30.issue-4
[14]	LI Z J, WANG D, SUI P, LONG P, YAN L L, WANG X L, YAN P, SHEN Y W, DAI H C, YANG X L, CUI J X, CHEN Y Q. Effects of different agricultural organic wastes on soil GHG emissions: During a 4-year field measurement in the North China Plain. Waste Management, 2018, 81: 202-210. doi: S0956-053X(18)30618-4 pmid: 30527036
[15]	JIANG Y, QIAN H Y, HUANG S, ZHANG X Y, WANG L, ZHANG L, SHEN M X, XIAO X P, CHEN F, ZHANG H L, LU C Y, LI C, ZHANG J, DENG A X, VAN GROENIGEN K J, ZHANG W J. Acclimation of methane emissions from rice paddy fields to straw addition. Science Advances, 2019, 5(1): 9038. doi: 10.1126/sciadv.aau9038 pmid: 30746466
[16]	LIU C, LU M, CUI J, LI B, FANG C M. Effects of straw carbon input on carbon dynamics in agricultural soils: A meta-analysis. Global Change Biology, 2014, 20(5): 1366-1381. doi: 10.1111/gcb.12517 pmid: 24395454
[17]	ZHANG A F, CHENG G, HUSSAIN Q, ZHANG M, FENG H, DYCK M, SUN B H, ZHAO Y, CHEN H X, CHEN J, WANG X D. Contrasting effects of straw and straw-derived biochar application on net global warming potential in the Loess Plateau of China. Field Crops Research, 2017, 205: 45-54. doi: 10.1016/j.fcr.2017.02.006
[18]	WANG N, YU J G, ZHAO Y H, CHANG Z Z, SHI X X, MA L Q, LI H B. Straw enhanced CO₂ and CH₄ but decreased N₂O emissions from flooded paddy soils: Changes in microbial community compositions. Atmospheric Environment, 2018, 174: 171-179. doi: 10.1016/j.atmosenv.2017.11.054
[19]	程功, 刘廷玺, 李东方, 段利民, 王冠丽. 生物炭和秸秆还田对干旱区玉米农田土壤温室气体通量的影响. 中国生态农业学报(中英文), 2019(7): 1004-1014.
	CHENG G, LIU T X, LI D F, DUAN L M, WANG G L. Effects of biochar and straw on greenhouse gas fluxes of corn fields in arid regions. Chinese Journal of Eco-Agriculture, 2019(7): 1004-1014. (in Chinese)
[20]	LIU P, HE J, LI H W, WANG Q J, LU C Y, ZHENG K, LIU W Z, ZHAO H B, LOU S Y. Effect of straw retention on crop yield, soil properties, water use efficiency and greenhouse gas emission in China: A meta-analysis. International Journal of Plant Production, 2019, 13(4): 347-367. doi: 10.1007/s42106-019-00060-w
[21]	LIU X, MAO P N, LI L H, MA J. Impact of biochar application on yield-scaled greenhouse gas intensity: A meta-analysis. Science of the Total Environment, 2019, 656: 969-976. doi: 10.1016/j.scitotenv.2018.11.396
[22]	成功, 陈静, 刘晶晶, 张阿凤, 王旭东, 冯浩, 赵英. 秸秆/生物炭施用对关中地区小麦-玉米轮作系统净增温潜势影响的对比分析. 环境科学, 2017, 38(2): 792-801.
	CHENG G, CHEN J, LIU J J, ZHANG A F, WANG X D, FENG H, ZHAO Y. Comparative analysis on effect of wheat straw and its biochar amendment on net global warming potential under wheat- maize rotation ecosystem in the Guanzhong Plain. Environmental Science, 2017, 38(2): 792-801. (in Chinese)
[23]	向伟, 王雷, 刘天奇, 李诗豪, 翟中兵, 李成芳. 生物炭与无机氮配施对稻田温室气体排放及氮肥利用率的影响. 中国农业科学, 2020, 53(22): 4634-4645. doi: 10.3864/j.issn.0578-1752.2020.22.010.
	XIANG W, WANG L, LIU T Q, LI S H, ZHAI Z B, LI C F. Effects of biochar plus inorganic nitrogen on the greenhouse gas and nitrogen use efficiency from rice fields. Scientia Agricultura Sinica, 2020, 53(22): 4634-4645. doi: 10.3864/j.issn.0578-1752.2020.22.010. (in Chinese)
[24]	李桂花, 郭俊娒, 姜慧敏, 张建峰. 有机肥和秸秆炭分别替代部分尿素和秸秆降低黑土温室效应的效果. 植物营养与肥料学报, 2018, 24(6):1566-1573.
	LI G H, GUO J M, JIANG H M, ZHANG J F. Partal substitution of urea and maize straw with manure and straw biochar decrease net greenhouse effect in black soil. Journal of Plant Nutrition and Fertilizers, 2018, 24(6): 1566-1573. (in Chinese)
[25]	万小楠, 赵珂悦, 吴雄伟, 白鹤, 杨学云, 顾江新. 秸秆还田对冬小麦-夏玉米农田土壤固碳、氧化亚氮排放和全球增温潜势的影响. 环境科学, 2022, 43(1): 569-576.
	WAN X N, ZHAO K Y, WU X W, BAI H, YANG X Y, GU J X. Effects of stalk incorporation on soil carbon sequestration, nitrous oxide emissions, and global warming potential of a winter wheat-summer maize field in Guanzhong plain. Environmental Science, 2022, 43(1): 569-576. (in Chinese)
[26]	XIA L L, WANG S W, YAN X Y. Effects of long-term straw incorporation on the net global warming potential and the net economic benefit in a rice-wheat cropping system in China. Agriculture, Ecosystems & Environment, 2014, 197: 118-127. doi: 10.1016/j.agee.2014.08.001
[27]	蔡育蓉, 王立刚. 北方典型农业生态系统的固碳减排路径及模式. 中国生态农业学报(中英文), 2022(4): 641-650.
	CAI Y R, WANG L G. Carbon sequestration and greenhouse gas mitigation paths and modes in a typical agroecosystem in Northern China. Chinese Journal of Eco-Agriculture, 2022(4): 641-650. (in Chinese)
[28]	王玉英, 李晓欣, 董文旭, 张玉铭, 秦树平, 胡春胜. 华北平原农田温室气体排放与减排综述. 中国生态农业学报, 2018, 26(2): 167-174.
	WANG Y Y, LI X X, DONG W X, ZHANG Y M, QIN S P, HU C S. Review on greenhouse gas emission and reduction in wheat-maize double cropping system in the North China Plain. Chinese Journal of Eco-Agriculture, 2018, 26(2): 167-174. (in Chinese)
[29]	SHI Y L, LIU X R, ZHANG Q W, LI G C, WANG P H. Biochar rather than organic fertilizer mitigated the global warming potential in a saline-alkali farmland. Soil and Tillage Research, 2022, 219: 105337. doi: 10.1016/j.still.2022.105337
[30]	HUANG Y, ZHANG W, SUN W J, ZHENG X H. Net primary production of Chinese croplands from 1950 to 1999. Ecological Applications, 2007, 17(3): 692-701. pmid: 17494389
[31]	朱晓晴, 安晶, 马玲, 陈松岭, 李嘉琦, 邹洪涛, 张玉龙. 秸秆还田深度对土壤温室气体排放及玉米产量的影响. 中国农业科学, 2020, 53(5): 977-989. doi: 10.3864/j.issn.0578-1752.2020.05.010.
	ZHU X Q, AN J, MA L, CHEN S L, LI J Q, ZOU H T, ZHANG Y L. Effects of different straw returning depths on soil greenhouse gas emission and maize yield. Scientia Agricultura Sinica, 2020, 53(5): 977-989. doi: 10.3864/j.issn.0578-1752.2020.05.010. (in Chinese)
[32]	LIU L, SHEN G Q, SUN M X, CAO X D, SHANG G F, CHEN P. Effect of biochar on nitrous oxide emission and its potential mechanisms. Journal of the Air & Waste Management Association, 2014, 64(8): 894-902.
[33]	WANG Y J, GUO J H, VOGT R D, MULDER J, WANG J G, ZHANG X S. Soil pH as the chief modifier for regional nitrous oxide emissions: new evidence and implications for global estimates and mitigation. Global Change Biology, 2018, 24(2): e617-e626.
[34]	AAMER M, SHAABAN M, HASSAN M U, LIU Y, TANG H Y, MA Q Y, MUNIR H, RASHEED A, LI X M, LI P, HUANG G Q. N₂O emissions mitigation in acidic soil following biochar application under different moisture regimes. Journal of Soil Science and Plant Nutrition, 2020, 20(4): 2454-2464. doi: 10.1007/s42729-020-00311-0
[35]	NIU Y H, CHEN Z M, MÜLLER C, ZAMAN M M, KIM D, YU H Y, DING W X. Yield-scaled N₂O emissions were effectively reduced by biochar amendment of sandy loam soil under maize - wheat rotation in the North China Plain. Atmospheric Environment, 2017, 170: 58-70. doi: 10.1016/j.atmosenv.2017.09.050
[36]	DUAN P P, ZHANG X, ZHANG Q Q, WU Z, XIONG Z Q. Field-aged biochar stimulated N₂O production from greenhouse vegetable production soils by nitrification and denitrification. Science of the Total Environment, 2018, 642: 1303-1310. doi: 10.1016/j.scitotenv.2018.06.166
[37]	WU Z, ZHANG X, DONG Y B, LI B, XIONG Z Q. Biochar amendment reduced greenhouse gas intensities in the rice-wheat rotation system: Six-year field observation and meta-analysis. Agricultural and Forest Meteorology, 2019, 278: 107625. doi: 10.1016/j.agrformet.2019.107625
[38]	LIAO X, MÜLLER C, JANSEN-WILLEMS A, LUO J F, LINDSEY S, LIU D Y, CHEN Z M, NIU Y H, DING W X. Field-aged biochar decreased N₂O emissions by reducing autotrophic nitrification in a sandy loam soil. Biology and Fertility of Soils, 2021, 57(4): 471-483. doi: 10.1007/s00374-021-01542-8
[39]	WU Y Z, LI Y, WANG H H, WANG Z J, FU X Q, SHEN J L, WANG Y, LIU X L, MENG L, WU J S. Response of N₂O emissions to biochar amendment on a tea field soil in subtropical central China: A three-year field experiment. Agriculture, Ecosystems & Environment, 2021, 318: 107473. doi: 10.1016/j.agee.2021.107473
[40]	LIAO X, NIU Y H, LIU D Y, CHEN Z M, HE T H, LUO J F, LINDSEY S, DING W X. Four-year continuous residual effects of biochar application to a sandy loam soil on crop yield and N₂O and NO emissions under maize-wheat rotation. Agriculture, Ecosystems & Environment, 2020, 302: 107109. doi: 10.1016/j.agee.2020.107109
[41]	HU N J, CHEN Q, ZHU L Q. The responses of soil N₂O emissions to residue returning systems: A meta-analysis. Sustainability, 2019, 11(3): 748. doi: 10.3390/su11030748
[42]	方明, 任天志, 赖欣, 王知文, 宋婷婷, 李洁, 张贵龙. 花生壳生物炭对潮土和红壤理化性质和温室气体排放的影响. 农业环境科学学报, 2018, 37(6): 1300-1310.
	FANG M, REN T Z, LAI X, WANG Z W, SONG T T, LI J, ZHANG G L. Effects of peanut shell biochar on physico-chemical properties and greenhouse gas emission in fluvo-aquic soil and red soil. Journal of Agro-Environment Science, 2018, 37(6): 1300-1310. (in Chinese)
[43]	TANG Y, GAO W C, CAI K, CHEN Y, LI C B, LEE X Q, CHENG H G, ZHANG Q H, CHENG J Z. Effects of biochar amendment on soil carbon dioxide emission and carbon budget in the Karst region of southwest China. Geoderma, 2021, 385: 114895. doi: 10.1016/j.geoderma.2020.114895
[44]	张玉兰, 曹明明, 王俊宇, 于清军. 生物炭在土壤中矿化的研究进展. 沈阳农业大学学报, 2017, 48(4): 482-487.
	ZHANG Y L, CAO M M, WANG J Y, YU Q J. Research on mineralization characteristics of biochar in soil. Journal of Shenyang Agricultural University, 2017, 48(4): 482-487. (in Chinese)
[45]	THOMAZINI A, SPOKAS K, HALL K, IPPOLITO J, LENTZ R, NOVAK J. GHG impacts of biochar: Predictability for the same biochar. Agriculture, Ecosystems & Environment, 2015, 207: 183-191. doi: 10.1016/j.agee.2015.04.012
[46]	HE Y H, ZHOU X H, JIANG L L, LI M, DU Z G, ZHOU G Y, SHAO J J, WANG X H, XU Z H, BAI S H, WALLACE H, XU C Y. Effects of biochar application on soil greenhouse gas fluxes: A meta-analysis. GCB Bioenergy, 2017, 9(4): 743-755. doi: 10.1111/gcbb.2017.9.issue-4
[47]	SUN L Y, LI L, CHEN Z Z, WANG J Y, XIONG Z Q. Combined effects of nitrogen deposition and biochar application on emissions of N₂O, CO₂ and NH₃ from agricultural and forest soils. Soil Science and Plant Nutrition, 2014, 60(2): 254-265. doi: 10.1080/00380768.2014.885386
[48]	SHENG Y Q, ZHU L Z. Biochar alters microbial community and carbon sequestration potential across different soil pH. Science of the Total Environment, 2018, 622/623: 1391-1399. doi: 10.1016/j.scitotenv.2017.11.337
[49]	张叶叶, 莫非, 韩娟, 温晓霞, 廖允成. 秸秆还田下土壤有机质激发效应研究进展. 土壤学报, 2021, 58(6): 1381-1392.
	ZHANG Y Y, MO F, HAN J, WEN X X, LIAO Y C. Research progress on the native soil carbon priming after straw addition. Acta Pedologica Sinica, 2021, 58(6): 1381-1392. (in Chinese)
[50]	ZHANG X B, WU L H, SUN N, DING X S, LI J W, WANG B R, LI D C. Soil CO₂ and N₂O emissions in maize growing season under different fertilizer regimes in an upland red soil region of South China. Journal of Integrative Agriculture, 2014, 13(3): 604-614. doi: 10.1016/S2095-3119(13)60718-2
[51]	BAMMINGER C, POLL C, MARHAN S. Offsetting global warming-induced elevated greenhouse gas emissions from an arable soil by biochar application. Global Change Biology, 2018, 24(1): e318-e334.
[52]	XU H, CAI A D, WU D, LIANG G P, XIAO J, XU M G, COLINET G, ZHANG W J. Effects of biochar application on crop productivity, soil carbon sequestration, and global warming potential controlled by biochar C: N ratio and soil pH: A global meta-analysis. Soil and Tillage Research, 2021, 213: 105125. doi: 10.1016/j.still.2021.105125
[53]	WANG J Y, XIONG Z Q, KUZYAKOV Y. Biochar stability in soil: Meta-analysis of decomposition and priming effects. GCB Bioenergy, 2016, 8(3): 512-523. doi: 10.1111/gcbb.2016.8.issue-3
[54]	BLANCO-CANQUI H, LAIRD D A, HEATON E A, RATHKE S, ACHARYA B S. Soil carbon increased by twice the amount of biochar carbon applied after 6 years: Field evidence of negative priming. GCB Bioenergy, 2020, 12(4): 240-251. doi: 10.1111/gcbb.v12.4
[55]	BAI S J, LIAN L, MA X. A study on the net greenhouse gas emissions under intensive high-yielding cropland in north China-A case study of winter wheat-summer maize rotation system in Huantai County. Energy Procedia, 2011, 5: 785-792. doi: 10.1016/j.egypro.2011.03.138
[56]	LIU W S, LIU W X, KAN Z R, CHEN J S, ZHAO X, ZHANG H L. Effects of tillage and straw management on grain yield and SOC storage in a wheat-maize cropping system. European Journal of Agronomy, 2022, 137: 126530. doi: 10.1016/j.eja.2022.126530
[57]	WANG Y L, WU P N, MEI F J, LING Y, QIAO Y B, LIU C S, LEGHARI S J, GUAN X K, WANG T C. Does continuous straw returning keep China farmland soil organic carbon continued increase? A meta-analysis. Journal of Environmental Management, 2021, 288: 112391. doi: 10.1016/j.jenvman.2021.112391
[58]	LI Y E, SHI S W, WAQAS M A, ZHOU X X, LI J L, WAN Y F, QIN X B, GAO Q Z, LIU S, WILKES A. Long-term (≥20 years) application of fertilizers and straw return enhances soil carbon storage: A meta-analysis. Mitigation and Adaptation Strategies for Global Change, 2018, 23(4): 603-619. doi: 10.1007/s11027-017-9751-2
[59]	LIU Y J, BI Y C, XIE Y X, ZHAO X, HE D X, WANG S Q, WANG C Y, GUO T C, XING G X. Successive straw biochar amendments reduce nitrous oxide emissions but do not improve the net ecosystem economic benefit in an alkaline sandy loam under a wheat-maize cropping system. Land Degradation & Development, 2020, 31(7): 868-883. doi: 10.1002/ldr.v31.7
[60]	GUO T F, LUAN H A, SONG D L, ZHANG S Q, ZHOU W, LIANG G Q. Combined fertilization could increase crop productivity and reduce greenhouse gas intensity through carbon sequestration under rice-wheat rotation. Agronomy, 2021, 11(12): 2540. doi: 10.3390/agronomy11122540
[61]	QI L, POKHAREL P, CHANG S X, ZHOU P, NIU H D, HE X H, WANG Z F, GAO M. Biochar application increased methane emission, soil carbon storage and net ecosystem carbon budget in a 2-year vegetable-rice rotation. Agriculture, Ecosystems & Environment, 2020, 292: 106831. doi: 10.1016/j.agee.2020.106831
[62]	LIN X W, XIE Z B, ZHENG J Y, LIU Q, BEI Q C, ZHU J G. Effects of biochar application on greenhouse gas emissions, carbon sequestration and crop growth in coastal saline soil. European Journal of Soil Science, 2015, 66(2): 329-338. doi: 10.1111/ejss.2015.66.issue-2
[63]	LI N, WEN S Y, WEI S K, LI H Y, FENG Y Z, REN G X, YANG G H, HAN X H, WANG X J, REN C J. Straw incorporation plus biochar addition improved the soil quality index focused on enhancing crop yield and alleviating global warming potential. Environmental Technology & Innovation, 2021, 21: 101316.

试验处理 Treatment	产量 Yield (t·hm^-2·a^-1)	ΔSOC (t C·hm^-2·a^-1)	NGWP (t CO₂-eq·hm^-2·a^-1)	GHGI (t CO₂-eq·t^-1)
CK	12.32±0.09a	0.62±0.03d	-1.05±0.07a	-0.12±0.01a
C	12.93±0.44a	1.04±0.04c	-2.76±0.24b	-0.42±0.02b
S	13.35±0.31a	1.17±0.06b	-2.84±0.35b	-0.44±0.04b
CS	13.58±0.18a	1.55±0.04a	-3.90±0.17c	-0.61±0.02c