栽培种花生株型相关性状的QTL定位

doi:10.3864/j.issn.0578-1752.2021.08.003

中国农业科学 ›› 2021, Vol. 54 ›› Issue (8): 1599-1612.doi: 10.3864/j.issn.0578-1752.2021.08.003

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

栽培种花生株型相关性状的QTL定位

孟鑫浩¹(),邓洪涛¹(),李丽²,崔顺立¹,Charles Y.Chen³,侯名语¹,杨鑫雷¹(),刘立峰¹()

¹河北农业大学农学院/华北作物改良与调控国家重点实验室/河北省种质资源实验室,中国河北保定 071001
²河北工程大学园林与生态工程学院,中国河北邯郸 056038
³奥本大学/作物、土壤与环境科学系,美国奥本 36849

收稿日期:2020-09-23 接受日期:2020-12-07 出版日期:2021-04-16 发布日期:2021-04-25
通讯作者: 杨鑫雷,刘立峰
作者简介:孟鑫浩,E-mail： mxinhao1994@126.com。|邓洪涛,E-mail： 3462096839@qq.com。
基金资助:
国家现代农业产业技术体系建设项目(CARS-13);国家自然科学基金(31701459,31771833);河北省科技计划(16226301D);河北省现代农业产业技术体系油料创新团队项目(HBCT2018090202);河北省青年拔尖人才资助项目(0602015);河北农业大学大学生创新创业训练计划(2018138)

QTL Mapping for Lateral Branch Angle Related Traits of Cultivated Peanut (Arachis hypogaea L.)

MENG XinHao¹(),DENG HongTao¹(),LI Li²,CUI ShunLi¹,Charles Y. CHEN³,HOU MingYu¹,YANG XinLei¹(),LIU LiFeng¹()

¹College of Agronomy, Hebei Agricultural University/State Key Laboratory of North China for Crop Improvement and Regulation/Key laboratory of Crop Germplasm Resources of Hebei Province, Baoding 071001, Hebei, China
²College of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, Hebei, China
³Department of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL 36849, USA

Received:2020-09-23 Accepted:2020-12-07 Online:2021-04-16 Published:2021-04-25
Contact: XinLei YANG,LiFeng LIU

摘要/Abstract

摘要：

【目的】栽培种花生是世界范围内重要的油料作物和经济作物,其株型相关性状是典型的数量性状,亦是重要的农艺性状,与产量和机械化收获密切相关。对花生株型相关性状进行遗传分析和QTL定位,筛选与之紧密连锁的分子标记,有助于花生的品种保护和品种鉴别,为花生株型分子育种提供重要的理论依据。【方法】以直立型花生品种冀花5号和匍匐型M130为亲本构建的包含321个家系的RIL群体为研究材料,于2016—2018年分别在海南市、邯郸市、保定市和唐山市等7个环境下种植,各个环境均在收获时调查统计花生侧枝夹角、主茎高、侧枝长、株型指数和扩展半径等5个株型相关性状的表型值。同时,利用SSR、AhTE、SRAP和TRAP等分子标记扫描亲本及群体的基因型用于构建分子遗传连锁图谱。最后结合多年多点的表型数据,采用QTL Icimapping V4.2中的完备区间作图法（inclusive composite interval mapping,ICIM）对7个环境下的株型相关性状进行加性QTL和上位性QTL分析。【结果】构建了一张包含363个多态性位点的分子遗传连锁图谱,所有标记被分配到20条染色体和1个未知连锁群;图谱总长度覆盖全基因组的1 360.38 cM,标记间平均距离为3.75 cM;单个连锁群长度为39.599—101.056 cM,包括4—50个分子标记。共检测到30个与株型相关性状的加性QTL,分布在A04、A05、A06、A08、A09、B02、B09等7条染色体上。其中,5个QTL与侧枝夹角相关,可解释的表型变异（phenotypic variance explained,PVE）为3.48%—11.22%;15个QTL与主茎高相关,PVE为3.58%—10.05%;2个QTL与侧枝长相关,PVE为6.03%—8.56%;4个QTL与株型指数相关,PVE为4.68%—15.08%;4个QTL与扩展半径相关,PVE为3.30%—9.33%。qLBAA05.1、qLBAA05.2、qMSHA04.2和qIOPTA05.1等4个主效QTL,可解释的表型变异分别为11.22%、10.59%、10.23%、10.05%和15.08%。此外,共检测到59对上位性QTL。其中,6对上位性QTL与侧枝长相关,PVE为2.23%—2.78%;50对上位性QTL与株型指数相关,PVE为0.25%—1.44%;3对上位性QTL与扩展半径相关,PVE为7.28%—12.25%。发现3个QTL聚集区,分别为A04染色体的GM1867—AHGS1967区间、A05染色体的me14em5-116—PM418区间和A08染色体的HBAUAh177—AhTE0658区间,涉及侧枝夹角、主茎高、株型指数和扩展半径等4个株型相关性状。【结论】构建了一张包含363个标记位点的分子遗传连锁图谱;获得30个与株型相关性状的加性QTL和59对上位性QTL;发现3个QTL聚集区。

关键词: 花生, 株型, QTL, 重组自交系

Abstract:

【Objective】Cultivated peanut (Arachis hypogaea L.) is an important oil and economic crop in worldwide. Plant type is a typical quantitative trait and an important agronomic trait, which is closely related to yield and mechanized harvesting in peanut. Genetic analysis, QTL mapping and identifying tightly linked molecular markers of plant type, will be conducive to the germplasm protection and cultivar identification, and provide an important theoretical basis for the molecular breeding of plant type in cultivated peanut. 【Method】In the present study, a RIL population as research material was established, which consisted of 321 families and derived from Jihua 5 with erect plant type and M130 with prostrate type. Two parents and RIL population were planted at Hainan city, Handan city, Baoding city and Tangshan city during the growing season (May to September) from 2016 to 2018. The phenotypic data of plant type related traits, such as lateral branch angle, main stem height, lateral branch length, index of plant type and extension radius, were investigated at harvesting season under seven environments. Meanwhile, SSR, AhTE, SRAP and TRAP were used to identify genotypic data of parents and RIL that was applied to construct the molecular genetic linkage map. Later, we combined phenotypic data of seven environments, and identified QTLs for plant type related traits using ICIM of QTL Icimapping V4.2. 【Result】A molecular genetic linkage map containing 363 polymorphism sites was constructed, and all markers were assigned to 20 chromosomes and an unknown linkage group. The total length of the map covered 1 360.38 cM of the whole genome, and the average distance between the markers was 3.75 cM. The length of a single linkage group was 39.599-101.056 cM, including 4-50 molecular markers. Subsequently, 30 additive QTLs for plant type related traits were detected by ICIM-ADD method, which were distributed on A04, A05, A06, A08, A09, B02 and B09 chromosomes. Among these QTLs, 5 QTLs for LBA with PVE was 3.48%-11.22%, 15 QTLs for MSH with PVE was 3.58%-10.05%, 2 QTLs for LBL with PVE was 6.03%-8.56%, 4 QTLs for IOPT with PVE was 4.68%-15.08%, 4 QTLs for ER with PVE was 3.30%-9.33%. Of these, qLBAA05.1, qLBAA05.2, qMSHA04.2, and qIOPTA05.1 were main-effect QTLs, explaining 11.22%, 10.59%, 10.23%, 10.05% and 15.08% of the phenotypic variance, respectively. In addition, 59 pairs epistatic QTLs were detected by ICIM-EPI method. Among them, 6 pairs of epistatic QTLs for LBL with PVE were 2.23% to 2.78%, 50 pairs of epistatic QTLs for IOPT with PVE were 0.25% to 1.44%, and 3 pairs of epistatic QTLs for ER with PVE were 7.28% to 12.25%. Finally, we also found 3 QTL clusters for LBA, MSH, IOPT and ER on GM1867-AHGS1967 interval of A04, me14em5-116-PM418 interval of A05 and HBAUAh177-AhTE0658 interval of A08, respectively. 【Conclusion】In brief, we constructed a molecular genetic linkage map containing 363 loci, and identified 30 additive QTLs and 59 pairs of epistatic QTLs for plant type related traits, and found 3 QTL clusters.

Key words: peanut, plant type, QTL, RILs

孟鑫浩,邓洪涛,李丽,崔顺立,Charles Y.Chen,侯名语,杨鑫雷,刘立峰. 栽培种花生株型相关性状的QTL定位[J]. 中国农业科学, 2021, 54(8): 1599-1612.

MENG XinHao,DENG HongTao,LI Li,CUI ShunLi,Charles Y. CHEN,HOU MingYu,YANG XinLei,LIU LiFeng. QTL Mapping for Lateral Branch Angle Related Traits of Cultivated Peanut (Arachis hypogaea L.)[J]. Scientia Agricultura Sinica, 2021, 54(8): 1599-1612.

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https://www.chinaagrisci.com/CN/Y2021/V54/I8/1599

图/表 8

图1

表1

亲本及RIL群体表型数据统计分析"

性状 Trait	环境 Env.	亲本 Parent		RIL群体RILs population
性状 Trait	环境 Env.	冀花5号 Jihua 5	M130	最小值 Minimum	最大值 Maximum	平均值 Mean	标准差 SD	变异系数 CV (%)	Shapiro- wilk (w)	偏度 Skewness	峰度 Kurtosis
侧枝夹角 LBA (°)	16BD	45.93	81.22	34.15	87.08	57.61	11.64	20.20	0.955***	0.330	-0.993
	16HN	37.41	77.05	34.03	80.07	55.90	11.75	21.02	0.953***	0.362	-1.094
	17BD	43.50	83.43	33.07	87.52	60.47	12.80	21.17	0.964***	0.277	-1.050
	17HD	39.20	79.01	35.35	90.10	58.63	13.09	22.33	0.949***	0.344	-1.128
	18BD	37.23	72.47	39.01	85.73	65.93	7.64	11.58	0.996	-0.344	0.408
	18HD	45.30	81.83	35.05	88.38	58.78	10.53	17.91	0.989	0.252	-0.246
	18TS	47.50	78.73	36.05	89.00	58.93	9.87	16.76	0.989	0.327	-0.136
主茎高 MSH (cm)	16BD	45.05	23.71	19.10	59.57	36.33	6.97	19.18	0.988**	0.348	0.221
	16HN	35.05	17.31	10.36	45.00	22.67	5.82	25.66	0.969***	0.702	0.415
	17BD	38.40	19.37	10.15	56.62	32.25	7.17	22.25	0.996	0.120	-0.038
	17HD	45.26	25.52	13.37	52.34	34.30	7.62	22.22	0.991	-0.039	-0.481
	18BD	38.00	19.70	10.71	52.52	27.96	7.94	28.38	0.977**	0.475	0.164
	18HD	45.05	23.36	12.28	55.76	34.89	8.04	23.04	0.992	-0.102	-0.087
	18TS	45.23	24.38	17.06	51.10	33.87	6.69	19.74	0.99	0.161	-0.297
侧枝长 LBL (cm)	16BD	49.76	61.42	24.56	75.71	49.20	7.56	15.36	0.995	0.144	0.642
	16HN	38.81	36.26	23.52	70.33	38.13	6.51	17.08	0.966***	0.689	2.532
	17BD	40.36	46.26	19.43	76.87	42.85	9.30	21.69	0.991	0.534	0.954
	17HD	47.68	55.30	23.00	83.00	49.71	8.74	17.59	0.979**	0.409	0.576
	18BD	41.00	76.71	14.27	87.81	39.25	10.13	25.80	0.996	0.462	1.660
	18HD	49.76	61.38	25.52	79.31	49.52	9.01	18.19	0.988	0.316	0.623
	18TS	51.53	61.67	24.05	90.00	44.83	8.29	18.50	0.955***	0.813	2.377
株型指数 IOPT	16BD	1.02	2.76	0.95	2.75	1.39	0.27	19.67	0.932***	1.174	1.470
	16HN	1.01	2.21	1.05	3.27	1.73	0.38	21.80	0.958***	0.892	1.533
	17BD	1.05	2.23	0.76	2.86	1.37	0.34	24.63	0.93***	1.197	1.632
	17HD	1.03	2.29	1.01	3.02	1.51	0.39	25.96	0.879***	1.479	2.665
	18BD	1.07	3.79	0.68	3.45	1.46	0.40	27.27	0.933***	1.297	2.645
	18HD	1.05	2.76	1.02	3.51	1.48	0.39	26.41	0.829***	1.961	5.759
	18TS	1.15	2.42	1.06	2.57	1.34	0.29	21.41	0.917***	0.654	3.582
扩展半径 ER (cm)	16BD	8.52	22.35	7.75	27.06	16.50	4.49	27.20	0.974***	0.411	-0.747
	16HN	16.13	31.57	8.05	33.08	18.52	5.80	31.34	0.965***	0.593	-0.562
	17BD	9.37	21.30	5.02	25.06	15.05	3.98	26.46	0.99	0.181	-0.517
	17HD	10.29	19.39	6.51	33.05	15.89	5.32	33.47	0.971***	0.52	-0.451
	18BD	9.33	34.71	5.38	36.38	21.00	5.01	23.83	0.996	-0.035	0.345
	18HD	8.67	21.17	5.01	30.02	15.11	5.16	34.15	0.978**	0.477	-0.283
	18TS	9.50	24.13	5.71	34.05	15.07	5.49	36.44	0.961***	0.742	0.357

表1

表2

表3

图2

表4

株型相关性状的QTL定位结果"

性状 Trait	QTL	环境 Env.	染色体 Chr.	位置 Position	标记区间 Marker interval	LOD	贡献率 PVE (%)	加性效应 Additive
侧枝夹角 LBA	qLBAA05.1	16BD	A05	6.00	me14em5-116—PM418	7.44	11.22	-3.9527
		17BD	A05	6.00	me14em5-116—PM418	6.99	10.59	-4.2307
		18HD	A05	6.00	me14em5-116—PM418	6.37	8.17	-3.2618
	qLBAA05.2	16HN	A05	5.00	me14em5-116—PM418	5.37	8.62	-3.6198
	qLBAA05.2	17HD	A05	5.00	me14em5-116—PM418	7.22	10.23	-4.8094
	qLBAA09.1	18BD	A09	20.98	GM66—GM1076	3.13	4.34	-2.0477
	qLBAA09.2	16HN	A09	24.98	RN27A10—AHTE0122	2.91	3.48	-2.3139
	qLBAB09.1	18HD	B09	50.00	me5em5-100—me13em8-142	2.89	4.51	2.4198
主茎高 MSH	qMSHA04.1	17BD	A04	21.00	GM1867—AHGS1967	4.94	6.23	1.9109
	qMSHA04.2	18BD	A04	22.00	GM1867—AHGS1967	7.49	10.05	2.7157
	qMSHA04.3	18HD	A04	23.00	GM1867—AHGS1967	3.55	5.22	1.9577
	qMSHA04.4	16BD	A04	24.00	GM1867—AHGS1967	3.11	3.58	1.6317
	qMSHA04.5	16HN	A04	26.00	GM1867—AHGS1967	3.99	5.24	1.4725
	qMSHA04.6	17HD	A04	28.00	GM1867—AHGS1967	6.54	6.34	2.4193
	qMSHA05.1	16BD	A05	4.00	me14em5-116 - PM418	4.54	6.43	2.1285
	qMSHA05.2	17HD	A05	5.00	me14em5-116—PM418	4.73	5.26	2.1268
	qMSHA06.1	16HN	A06	36.41	TC7C06—AHTE0372	4.54	4.90	1.3824
	qMSHA06.1	17BD	A06	36.41	TC7C06—AHTE0372	3.46	4.23	1.5366
	qMSHA06.2	18BD	A06	46.21	TC7C06—AHTE0372	3.21	3.67	1.5995
	qMSHA08.1	17BD	A08	30.00	Ah4-4—TC9B08	3.58	4.62	-1.6048
	qMSHA08.2	18BD	A08	35.00	TC9B08—AHGS1947b	3.36	4.47	-1.7650
	qMSHB02.1	17HD	B02	25.00	AHTE0398—CTW_NEW_38	4.00	4.48	-1.9683
	qMSHB02.2	18HD	B02	29.00	AHTE0398—CTW_NEW_38	2.96	3.74	-1.6183
	qMSHB09.1	16HN	B09	22.00	AHGS1576—me11em4-144	7.92	9.44	-1.9154
侧枝长 LBL	qLBLA08.1	18BD	A08	8.40	HBAUAh177—AhTE0658	4.86	6.03	-3.2587
	qLBLA08.2	17BD	A08	9.12	HBAUAh177—AhTE0658	5.27	7.53	-2.5945
	qLBLA08.2	18HD	A08	9.12	HBAUAh177—AhTE0658	6.13	8.56	-2.6868
株型指数 IOPT	qIOPTA05.1	16BD	A05	4.00	me14em5-116—PM418	10.15	15.08	-0.1151
	qIOPTA05.1	17HD	A05	4.00	me14em5-116—PM418	5.18	9.35	-0.1308
	qIOPTA05.2	18TS	A05	5.00	me14em5-116—PM418	3.84	5.85	-0.0757
	qIOPTA05.3	17BD	A05	6.00	me14em5-116—PM418	6.25	8.58	-0.1006
	qIOPTA05.3	18BD	A05	6.00	me14em5-116—PM418	3.23	4.68	-0.1121
	qIOPTA05.4	18HD	A05	7.00	PM418—pPGSSeq11E11	4.48	6.47	-0.1012
扩展半径 ER	qERA05.1	16BD	A05	5.00	me14em5-116—PM418	6.63	9.33	-1.5728
		16HN	A05	5.00	me14em5-116—PM418	5.03	7.41	-1.7923
		17BD	A05	5.00	me14em5-116—PM418	3.16	3.86	-0.9281
		17HD	A05	5.00	me14em5-116—PM418	6.15	8.55	-1.8270
		18HD	A05	5.00	me14em5-116—PM418	5.14	7.38	-1.5946
	qERA05.2	18TS	A05	6.00	me14em5-116—PM418	3.96	5.92	-1.3923
	qERA08.1	17BD	A08	7.69	HBAUAh177—AhTE0658	3.01	3.30	-0.8612
	qERA08.2	18BD	A08	8.40	HBAUAh177—AhTE0658	5.18	5.53	-1.3478

表4

表5

表6

株型相关性状的上位性QTL定位结果"

性状 Trait	上位性QTL Epi-QTL	环境 Env.	染色体 Chr.	位置 Position	标记区间 Marker interval	染色体 Chr.	位置 Position	标记区间 Marker interval	LOD	贡献率 PVE (%)
侧枝长 LBL	Epi-qLBLA04.1	18BD	A04	15.000	ARS772—GM1867	A04	20.000	GM1867—AHGS1967	13.760		2.420
	Epi-qLBLA02.1	18BD	A02	35.000	AHGS1163—AHGS1886	A02	40.000	AHGS1886—AHGS1159	9.620		2.230
	Epi-qLBLA05.1	18BD	A05	35.000	AhTE0588—T5me8-66	A05	40.000	T5me8-66—T2me4-75	10.420		2.250
	Epi-qLBLA03.1	18BD	A03	25.000	GM1883—AhTE0570	A03	30.000	AhTE0570—TC4G02	11.900		2.280
	Epi-qLBLB03.1	18BD	B03	7.165	AHBGSC1003E10—GM1996	B03	8.609	AHBGSC1003E10—GM1996	9.750		2.230
	Epi-qLBLA08.1	18BD	A08	6.255	HBAUAh177—AhTE0658	A08	10.000	AhTE0658—TC6H03	7.810		2.780
株型指数 IOPT	Epi-qIOPTB04.1	16BD	B04	64.814	me2em16-141—me9em4-125	B04	69.814	me2em16-141—me9em4-125	8.920		1.360
	Epi-qIOPTB07.1	16BD	B07	5.000	TC1A08—TC9H09	B07	10.000	TC1A08—TC9H09	7.280		1.200
	Epi-qIOPTA02.4	16BD	A02	45.000	AHGS1886—AHGS1159	A02	50.000	AHGS1886—AHGS1159	8.510		1.420
	Epi-qIOPTA03.4	16BD	A03	40.000	AhTE0570—TC4G02	A03	45.000	AhTE0570—TC4G02	8.530		1.160
	Epi-qIOPTB03.1	16BD	B03	7.165	AHBGSC1003E10—GM1996	B03	17.351	GM1996—seq2H08	8.760		1.440
	Epi-qIOPTA10.2	16BD	A10	20.000	AhTE0586—AHGS1192	A10	25.000	AHGS1192—seq3e10	5.500		0.850
	Epi-qIOPTA04.5	17BD	A04	50.000	GM1135—pPGSseq15C12a	A04	55.000	GM1135—pPGSseq15C12a	8.300		0.800
	Epi-qIOPTB07.3	17BD	B07	10.000	TC1A08—TC9H09	B07	15.000	TC1A08—TC9H09	9.440		0.860
	Epi-qIOPTA02.3	17BD	A02	40.000	AHGS1886—AHGS1159	A02	45.000	AHGS1886—AHGS1159	8.670		0.850
	Epi-qIOPTA08.1	17BD	A08	10.827	me3em14-196—Ah4-4	A08	15.544	me3em14-196—Ah4-4	8.720		0.920
	Epi-qIOPTA05.1	17BD	A05	5.000	me7em9-96—me13em5-112	A05	10.000	me7em9-96—me13em5-112	6.020		0.810
	Epi-qIOPTA03.1	17BD	A03	25.000	GM1883—AhTE0570	A03	35.000	AhTE0570—TC4G02	7.840		0.720
	Epi-qIOPTA06.1	17BD	A06	10.164	me4em15-95—me7em1-83	A06	15.078	me4em15-95—me7em1-83	7.270		0.830
	Epi-qIOPTB03.2	17BD	B03	7.165	AHBGSC1003E10—GM1996	B03	17.351	GM1996—seq2H08	8.660		0.820
	Epi-qIOPTA10.3	17BD	A10	20.000	AhTE0586—AHGS1192	A10	25.000	AHGS1192—seq3e10	7.930		0.780
性状 Trait	上位性QTL Epi-QTL	环境 Env.	染色体 Chr.	位置 Position	标记区间 Marker interval	染色体 Chr.	位置 Position	标记区间 Marker interval	LOD	贡献率 PVE (%)
	Epi-qIOPTB07.6	17HD	B09	10.000	T3me2-388—AHGS1576	B09	15.000	T3me2-388—AHGS1576	10.480		1.360
	Epi-qIOPTA04.4	17HD	A04	40.000	AHGS1967—GM1135	A04	50.000	GM1135—pPGSseq15C12a	11.580		1.280
	Epi-qIOPTB07.4	17HD	B07	10.000	TC1A08—TC9H09	B07	15.000	TC1A08—TC9H09	10.810		1.330
	Epi-qIOPTA02.5	17HD	A02	50.000	AHGS1886—AHGS1159	A02	55.000	AHGS1886—AHGS1159	10.100		1.410
	Epi-qIOPTA05.2	17HD	A05	5.000	me14em5-116—PM418	A05	10.000	PM418—seq11E11	9.100		1.380
	Epi-qIOPTA08.4	17HD	A08	15.543	me3em14-196—Ah4-4	A08	20.260	me3em14-196—Ah4-4	11.350		1.360
	Epi-qIOPTA05.3	17HD	A05	5.000	me7em9-96—me13em5-112	A05	10.000	me7em9-96—me13em5-112	11.390		1.300
	Epi-qIOPTB07.5	17HD	B08	20.000	me4em5-88—AI119H15	B08	25.000	me4em5-88—AI119H15	8.550		1.230
	Epi-qIOPTA03.2	17HD	A03	30.000	AhTE0570—TC4G02	A03	35.000	AhTE0570—TC4G02	11.840		1.280
	Epi-qIOPTB03.3	17HD	B03	7.165	AHBGSC1003E10—GM1996	B03	8.609	AHBGSC1003E10—GM1996	14.290		1.430
	Epi-qIOPTA08.2	17HD	A08	15.000	AhTE0658—TC6H03	A08	20.000	TC6H03—AhTE0477	5.460		1.000
	Epi-qIOPTA04.2	17HD	A04	25.000	GM1867—AHGS1967	A08	25.000	TC6H03—AhTE0477	5.640		0.480
	Epi-qIOPTA10.4	17HD	A10	20.000	AhTE0586—AHGS1192	A10	25.000	AHGS1192—seq3e10	11.310		1.200
	Epi-qIOPTA04.3	18BD	A04	25.000	GM1867—AHGS1967	A04	30.000	GM1867—AHGS1967	27.990		0.900
	Epi-qIOPTA02.1	18BD	A02	20.000	Ah3TC13E05—AHGS1463	A02	25.000	AHGS1463—AHGS1163	25.850		0.760
	Epi-qIOPTA05.5	18BD	A05	25.000	seq11E11—AhTE0588	A05	30.000	AhTE0588—T5me8-66	25.060		0.770
	Epi-qIOPTA03.5	18BD	A03	40.000	AhTE0570—TC4G02	A03	45.000	AhTE0570—TC4G02	25.170		1.230
	Epi-qIOPTA06.2	18BD	A06	15.078	me4em15-95—me7em1-83	A06	20.000	me7em1-83—me8em16-92	25.390		0.780
	Epi-qIOPTB03.5	18BD	B03	31.542	seq2H08—GM1954	B03	31.236	GM1954—IPAHM103	24.970		0.770
	Epi-qIOPTA10.1	18BD	A10	15.000	AhTE0586—AHGS1192	A10	20.000	AhTE0586—AHGS1192	21.990		0.770
	Epi-qIOPTB07.7	18HD	B09	15.000	T3me2-388—AHGS1576	B09	20.000	T3me2-388—AHGS1576	13.540		0.870
	Epi-qIOPTA04.6	18HD	A04	70.000	pPGSseq15C12a—me7em17-133	A04	75.000	pPGSseq15C12a—me7em17-133	18.600		0.850
	Epi-qIOPTB07.2	18HD	B07	5.000	TC1A08—TC9H09	B07	10.000	TC1A08—TC9H09	13.000		0.890
	Epi-qIOPTA02.2	18HD	A02	35.000	AHGS1163—AHGS1886	A02	45.000	AHGS1886—AHGS1159	14.530		0.850
	Epi-qIOPTA05.6	18HD	A05	50.000	T2me4-75—seq18C2	A05	55.000	T2me4-75—seq18C2	16.160		0.950
	Epi-qIOPTA08.5	18HD	A08	20.260	me3em14-196—Ah4-4	A08	30.000	Ah4-4—TC9B08	12.330		0.830
	Epi-qIOPTA05.4	18HD	A05	15.000	me7em9-96—me13em5-112	A05	20.000	me7em9-96—me13em5-112	15.600		1.120
	Epi-qIOPTA03.6	18HD	A03	45.000	AhTE0570—TC4G02	A03	50.000	TC4G02—IPAHM93	15.850		0.840
	Epi-qIOPTA06.3	18HD	A06	35.000	me7em1-83—me8em16-92	A06	40.000	me8em16-92—AHGS1337	15.200		0.840
	Epi-qIOPTB03.4	18HD	B03	31.236	GM1954—IPAHM103	B03	45.139	GM1954—IPAHM103	19.630		0.840
	Epi-qIOPTA08.3	18HD	A08	15.000	AhTE0658—TC6H03	A08	20.000	TC6H03—AhTE0477	17.200		0.830
	Epi-qIOPTA10.5	18HD	A10	20.000	AhTE0586—AHGS1192	A10	25.000	AHGS1192—seq3e10	19.290		0.830
	Epi-qIOPTA04.1	18TS	A04	5.000	ARS772—GM1867	A04	10.000	ARS772—GM1867	5.060		0.260
	Epi-qIOPTA08.6	18TS	A08	20.260	me3em14-196—Ah4-4	A08	25.000	Ah4-4—TC9B08	5.410		0.260
	Epi-qIOPTA03.3	18TS	A03	30.000	AhTE0570—TC4G02	A03	35.000	AhTE0570—TC4G02	6.150		0.250
扩展半径 ER	Epi-qERA09.1	17BD	A09	25.976	AHTE0122—HBAUAh098	A08	10.827	me3em14-196—Ah4-4	5.830		12.110
	Epi-qERB04.2	18HD	B04	64.814	me2em16-141—me9em4-125	B08	5.000	AHGS1286—TC20B05	5.880		12.250
	Epi-qERB04.1	18TS	B04	24.814	AHTE0445—PM36	B04	29.814	PM36—PMc348	6.590		7.280

表6

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环境Env.	性状Trait	侧枝夹角LBA	主茎高MSH	侧枝长LBL	株型指数IOPT
16BD	主茎高MSH	-0.323**
	侧枝长LBL	0.323**	0.456**
	株型指数IOPT	0.604**	-0.631**	0.374**
	扩展半径ER	0.820**	-0.262**	0.450**	0.646**
16HN	主茎高MSH	-0.127*
	侧枝长LBL	-0.009*	0.584**
	株型指数IOPT	0.205**	-0.706**	0.061*
	扩展半径ER	0.761**	0.086*	0.223**	0.129*
17BD	主茎高MSH	-0.278**
	侧枝长LBL	0.429**	0.450**
	株型指数IOPT	0.683**	-0.539**	0.473**
	扩展半径ER	0.736**	-0.093*	0.589**	0.651**
17HD	主茎高MSH	-0.372**
	侧枝长LBL	0.333**	0.394**
	株型指数IOPT	0.595**	-0.687**	0.349**
	扩展半径ER	0.820**	-0.270**	0.496**	0.638**
18BD	主茎高MSH	-0.118*
	侧枝长LBL	0.557**	0.547**
	株型指数IOPT	0.457**	-0.506**	0.408**
	扩展半径ER	0.815**	0.303**	0.748**	0.455**
18HD	主茎高MSH	-0.184**
	侧枝长LBL	0.458**	0.466**
	株型指数IOPT	0.537**	-0.666**	0.281**
	扩展半径ER	0.897**	-0.155**	0.546**	0.577**
18TS	主茎高MSH	-0.117*
	侧枝长LBL	0.380**	0.538**
	株型指数IOPT	0.465**	-0.532**	0.352**
	扩展半径ER	0.878**	-0.044	0.512**	0.518**

性状 Trait	变异来源 Variables	自由度 df	均方 MS	F值 F-value	P值 P-value	广义遗传力 h2_B
侧枝夹角 LBA	基因型Genotype	320	616.890	14.658	<0.01	0.86
	环境Environment	6	3124.760	74.247	<0.01
	基因型×环境G×E	1877	42.086	1.036	>0.05
主茎高 MSH	基因型Genotype	320	240.144	11.943	<0.01	0.92
	环境Environment	6	7341.320	365.118	<0.01
	基因型×环境G×E	1877	20.107	1.723	<0.01
侧枝长 LBL	基因型Genotype	320	270.164	6.924	<0.01	0.90
	环境Environment	6	7396.938	189.580	<0.01
	基因型×环境G×E	1779	39.017	1.836	<0.01
株型指数 IOPT	基因型Genotype	320	0.466	7.433	<0.01	0.91
	环境Environment	6	4.097	65.320	<0.01
	基因型×环境G×E	1779	0.063	1.896	<0.01
扩展半径 ER	基因型Genotype	320	121.227	12.600	<0.01	0.89
	环境Environment	6	1599.042	166.201	<0.01
	基因型×环境G×E	1881	9.621	2.265	<0.01

栽培种花生株型相关性状的QTL定位

QTL Mapping for Lateral Branch Angle Related Traits of Cultivated Peanut (Arachis hypogaea L.)

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染色体 Chr.	标记区间 Marker interval	QTL				LOD	贡献率 PVE (%)
A04	GM1867-A04—AHGS1967-A04	qMSHA04.1	qMSHA04.2	qMSHA04.3	qMSHA04.4	3.11—7.49	3.25—10.05
A04	GM1867-A04—AHGS1967-A04	qMSHA04.5	qMSHA04.6			3.11—7.49	3.25—10.05
A05	me14em5-116—PM418-A05	qLBAA05.1	qLBAA05.2	qMSHA05.1	qMSHA05.2	3.16—10.15	3.86—15.08
		qIOPTA05.1	qIOPTA05.2	qIOPTA05.3	qERA05.1
		qERA05.2
A08	HBAUAh177—AhTE0658-A08	qLBLA08.1	qLBLA08.2	qERA08.1	qERA08.2	3.01—6.13	3.30—8.56

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