四倍体小麦胚大小性状QTL定位与分析

doi:10.3864/j.issn.0578-1752.2023.02.001

中国农业科学 ›› 2023, Vol. 56 ›› Issue (2): 203-216.doi: 10.3864/j.issn.0578-1752.2023.02.001

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

四倍体小麦胚大小性状QTL定位与分析

陈吉浩^1,²(),周界光¹(),曲翔汝¹,王素容¹,唐华苹¹,蒋云³,唐力为⁴,$\boxed{\hbox{兰秀锦}}$¹,魏育明¹,周景忠⁵(),马建¹()

¹四川农业大学小麦研究所，成都 611130
²四川农业大学农学院，成都 611130
³四川省农业科学院生物技术核技术研究所，成都 610000
⁴攀枝花市农林科学研究院，四川攀枝花 617061
⁵通辽市农牧科学研究所，内蒙古通辽 028015

收稿日期:2022-07-05 接受日期:2022-08-12 出版日期:2023-01-16 发布日期:2023-02-07
通讯作者: 周景忠，E-mail：nmklzhou@163.com。马建，E-mail：jianma@sicau.edu.cn
作者简介:陈吉浩，E-mail：317093793@qq.com。|周界光，E-mail：351062153@qq.com。
基金资助:
国家农业重大科技项目(NK20220607);四川省科技计划(2021YFH0083);四川省科技计划(2022NSFSC1729);四川省科技计划(2022YFH0053)

Mapping and Analysis of QTL for Embryo Size-Related Traits in Tetraploid Wheat

CHEN JiHao^1,²(),ZHOU JieGuang¹(),QU XiangRu¹,WANG SuRong¹,TANG HuaPing¹,JIANG Yun³,TANG LiWei⁴,$\boxed{\hbox{LAN XiuJin}}$¹,WEI YuMing¹,ZHOU JingZhong⁵(),MA Jian¹()

¹Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130
²College of Agronomy, Sichuan Agricultural University, Chengdu 611130
³Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, 610000
⁴PanZhiHua Academy of Agricultural and Forestry Sciences, Panzhihua 617061, Sichuan
⁵Tongliao Institute of Agriculture and Animal Husbandry Sciences, Tongliao 028015, Inner Mongolia

Received:2022-07-05 Accepted:2022-08-12 Online:2023-01-16 Published:2023-02-07

摘要/Abstract

摘要：

【目的】挖掘小麦胚大小性状相关的数量性状位点，解析胚大小与其他重要农艺性状之间的相关性，为胚相关性状QTL的精细定位及育种利用奠定基础。【方法】以四倍体小麦矮兰麦（Ailanmai）和野生二粒小麦（LM001）构建的121份F₈代重组自交系群体（AM群体）作为研究材料，将其分别种植于成都市崇州试验基地（2018、2019和2020年）、成都市温江区试验基地（2020年）和雅安市试验基地（2020年），调查5个环境下的胚长、胚宽、胚长/胚宽、胚长/粒长、胚宽/粒宽以及胚面积6个性状，结合基于小麦55K SNP芯片构建的遗传连锁图谱对上述6个性状进行QTL定位。【结果】胚大小性状呈近似正态分布，符合数量性状的遗传特征。QTL定位共检测到27个胚大小相关性状的QTL，其中，7个分别控制胚长和胚宽的QTL可解释7.75%—21.74%和7.67%—33.29%的表型变异，共检测到5个在多环境稳定表达的主效QTL：QEL.sicau-AM-3B、QEW.sicau-AM-2B、QEW/KW.sicau-AM-2B、QEL/EW.sicau-AM-2B-1和QEA.sicau-AM-2B，其贡献率分别为11.88%—21.74%、21.77%—33.29%、8.80%—24.92%、12.79%—31.13%和10.47%—20.67%。另外，上述胚相关的位点形成4个QTL簇，分别为1B控制胚长/粒长和胚长的QTL簇，2B控制胚宽、胚长/胚宽、胚宽/粒宽以及胚面积的QTL簇，3B控制胚长和胚面积的QTL簇，6B控制胚长/胚宽、胚宽/粒宽的QTL簇。确定胚大小与小麦籽粒大小具有显著正相关性，且发现胚长主效位点QEL.sicau-AM-3B与粒长主效位点共定位，但胚宽主效位点QEW.sicau-AM-2B独立于粒宽主效位点存在。在主效QTL所在物理区间鉴定获得4个可能参与胚大小调控的基因。【结论】鉴定到5个控制胚相关性状的稳定表达的主效QTL:QEL.sicau-AM-3B、QEW.sicau-AM-2B、QEW/KW.sicau-AM-2B、QEL/EW.sicau-AM-2B-1和QEA.sicau-AM-2B，其中，QEW.sicau-AM-2B可能为新的QTL。

关键词: 四倍体小麦, 胚长, 胚宽, 胚面积, QTL定位

Abstract:

【Objective】 This study is to excavate embryo-related quantitative trait loci (QTL) with potential breeding value, to explore the genetic relationship between embryo and other agronomic traits in tetraploid wheat, and finally to aim at laying an important foundation for the fine mapping and breeding utilization of embryo-related traits in the future. 【Method】A total of 121 F₈ recombinant inbred lines (RIL) constructed by crossing tetraploid durum wheat (Ailanmai) and wild emmer wheat (LM001) were used. This RIL population was planted in five different environments including Chongzhou (2018-2020), Wenjiang (2020), and Ya'an (2020) in Sichuan Province for phenotypic evaluation of embryo length (EL), embryo width (EW), embryo length/embryo width (EL/EW), embryo length/kernel length (EL/KL), embryo width/kernel width (EW/KW), and embryo area (EA). QTL mapping was performed based on a genetic linkage map constructed based on the wheat 55K SNP. 【Result】 The embryo size-related traits showed approximately normal distribution in the RIL population satisfying the genetic characteristics of quantitative traits. A total of 27 QTL for embryo size-related traits were detected in five environments over three years. Among them, seven ones controlling EL could contribute 7.75%-21.74% of phenotypic variation. Seven QTLs controlling EW could explain 7.67%-33.29% of phenotypic variation. Five stable and major QTLs (QEL.sicau-AM-3B, QEW.sicau-AM-2B, QEW/KW.sicau-AM-2B, QEL/EW.sicau- AM-2B-1 and QEA.sicau-AM-2B) were identified, and they explained 11.88%-18.99%, 21.77%-29.41%, 8.80%-24.92%, 12.79%- 25.69% and 10.47%-15.22% of phenotypic variation, respectively. In addition, four QTL-rich regions were identified in the embryo size-related loci mentioned above. The QTL controlling EL/KL and EL was located on chromosome 1B, that for EW, EL/EW, EW/KW, and EA was located on 2B, that controlling EL and EA was on 3B, and that controlling EL/EW and EW/KW was on 6B. Embryo size was significantly and positively correlated with kernel size. Further, the major QTL for EL, QEL.sicau-AM-3B was co-located with that for kernel length identified previously, but that for EW QEW.sicau-AM-2B was independent of that for kernel width. Four genes likely involved in regulation of embryo size were identified in intervals where major QTL were mapped. 【Conclusion】Five stable and major QTLs were identified: QEL.sicau-AM-3B, QEW.sicau-AM-2B, QEW/KW.sicau-AM-2B, QEL/EW.sicau-AM-2B-1, QEA.sicau-AM-2B, among which QEW.sicau-AM-2B may be novel.

Key words: tetraploid wheat, embryo length, embryo width, embryo area, QTL mapping

陈吉浩, 周界光, 曲翔汝, 王素容, 唐华苹, 蒋云, 唐力为, $\boxed{\hbox{兰秀锦}}$, 魏育明, 周景忠, 马建. 四倍体小麦胚大小性状QTL定位与分析[J]. 中国农业科学, 2023, 56(2): 203-216.

CHEN JiHao, ZHOU JieGuang, QU XiangRu, WANG SuRong, TANG HuaPing, JIANG Yun, TANG LiWei, $\boxed{\hbox{LAN XiuJin}}$, WEI YuMing, ZHOU JingZhong, MA Jian. Mapping and Analysis of QTL for Embryo Size-Related Traits in Tetraploid Wheat[J]. Scientia Agricultura Sinica, 2023, 56(2): 203-216.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2023.02.001

https://www.chinaagrisci.com/CN/Y2023/V56/I2/203

图/表 9

表1

AM群体亲本及其RIL胚相关性状表型变异"

性状 Trait	环境 Environment	亲本 Parents		重组自交系群体 RIL population
性状 Trait	环境 Environment	Ailanmai	LM001	范围 Range	平均值 Mean	标准差 SD	变异系数 CV	偏度 Skewness	峰度 Kurtosis	广义遗传力 H²
EL (mm)	2020WJ	2.58	3.09a	2.13—3.99	3.20	0.31	0.10	-0.38	0.81	0.75
	2020YA	2.55	3.08a	2.14—4.00	2.94	0.41	0.14	0.82	0.13
	2020CZ	2.51	3.08a	2.29—3.99	3.06	0.36	0.12	0.37	-0.22
	2019CZ	2.64	3.10a	2.13—4.00	3.07	0.35	0.11	0.12	0.01
	2018CZ	2.43	2.75a	2.43—4.00	3.13	0.31	0.10	0.21	0.04
	BLUP	2.62	3.02	2.45—3.37	3.00	0.17	0.06	0.59	0.81
EW (mm)	2020WJ	1.95a	1.88	1.48—2.95	2.02	0.33	0.16	0.84	0.05	0.76
	2020YA	2.14a	1.93	1.51—2.94	1.98	0.30	0.15	0.94	0.65
	2020CZ	1.98b	1.91	1.40—3.00	2.08	0.36	0.17	0.66	-0.12
	2019CZ	2.19a	1.99	1.43—3.00	2.06	0.32	0.16	1.03	1.19
	2018CZ	1.90a	1.54	1.45—3.00	2.01	0.30	0.15	1.16	1.48
	BLUP	2.02	1.86	1.64—3.00	1.95	0.14	0.07	0.14	0.01
EL/KL	2020WJ	0.39b	0.36	0.31—0.49	0.38	0.03	0.08	0.85	0.64	0.37
	2020YA	0.38b	0.36	0.27—0.41	0.34	0.03	0.09	0.12	-0.29
	2020CZ	0.40b	0.37	0.29—0.48	0.35	0.03	0.09	0.93	3.09
	2019CZ	0.40b	0.36	0.29—0.49	0.36	0.03	0.08	0.93	2.12
	2018CZ	0.39a	0.34	0.34—0.49	0.38	0.03	0.08	1.21	1.42
	BLUP	0.37	0.36	0.33—0.38	0.35	0.01	0.03	-0.66	1.88
EW/KW	2020WJ	0.50b	0.56	0.42—0.71	0.54	0.06	0.11	0.27	-0.29	0.64
	2020YA	0.56	0.57	0.44—0.81	0.56	0.07	0.13	0.76	0.76
	2020CZ	0.50b	0.57	0.39—0.76	0.57	0.07	0.12	0.18	0.23
	2019CZ	0.56	0.58	0.39—0.69	0.54	0.06	0.11	0.01	-0.15
	2018CZ	0.49b	0.46	0.42—0.65	0.55	0.06	0.11	0.26	-0.19
	BLUP	0.53	0.55	0.47—0.65	0.55	0.03	0.05	0.22	0.14
EL/EW	2020WJ	1.33b	1.65	1.20—1.99	1.65	0.16	0.10	-0.41	0.16	0.72
	2020YA	1.20	1.60a	1.15—1.99	1.49	0.16	0.11	0.71	0.68
	2020CZ	1.28	1.62a	1.12—2.52	1.54	0.2	0.13	1.78	7.93
	2019CZ	1.23	1.56	1.16—2.14	1.53	0.17	0.11	0.37	0.92
	2018CZ	1.28b	1.79	1.28—2.16	1.6	0.16	0.10	0.33	0.58
	BLUP	1.31	1.63	1.20—1.78	1.55	0.11	0.07	-0.52	0.47
EA (mm²)	2020WJ	3.62a	4.17	2.44—6.26	4.39	0.69	0.16	0.10	-0.01	0.75
	2020YA	3.93a	4.29	2.56—6.35	4.07	0.81	0.20	0.59	-0.22
	2020CZ	3.56a	4.24	2.65—6.18	4.27	0.72	0.17	-0.05	-0.16
	2019CZ	4.16a	4.44	2.74—6.17	4.35	0.73	0.17	0.25	-0.38
	2018CZ	3.33b	3.04	2.76—6.63	4.33	0.71	0.16	0.39	0.32
	BLUP	3.80	4.07	3.22—5.48	4.22	0.46	0.11	-0.12	-0.21

表1

表2

图1

表3

表4

胚相关性状的QTL定位结果"

性状 Trait	数量性状位点 QTL	环境Environment	染色体 Chromosome	左标记 Left marker	右标记 Right marker	位置 Position (cM)	阈值 LOD	表型变异率 PVE (%)	加性效应 Add
胚长 EL	QEL.sicau-AM-3B	2020WJ	3B	AX-110375013	AX-110569582	6.91—7.34	5.15	16.21	-0.11
		2020YA	3B	AX-110375013	AX-110569582	6.91—7.34	3.63	11.88	-0.10
		2020CZ	3B	AX-111112626	AX-110375013	6.91—7.34	4.60	14.95	-0.10
		2019CZ	3B	AX-110375013	AX-110569582	6.91—7.34	4.90	18.99	-0.12
		BLUP	3B	AX-110375013	AX-110569582	6.91—7.34	8.41	21.74	-0.09
	QEL.sicau-AM-1B-1	2020CZ	1B	AX-108913437	AX-111510846	46.84—47.27	2.78	8.28	0.08
	QEL.sicau-AM-1B-1	BLUP	1B	AX-108913437	AX-111510846	46.84—47.27	4.22	9.77	0.06
	QEL.sicau-AM-1B-2	2020YA	1B	AX-111720572	AX-110579523	50.30—51.15	4.14	13.37	0.11
	QEL.sicau-AM-7B	2020WJ	7B	AX-110995675	AX-109951040	171.56—172.01	2.61	7.75	0.07
胚宽 EW	QEW.sicau-AM-2B	2020WJ	2B	AX-109390001	AX-110571221	0.00—1.92	7.42	24.13	0.10
		2020CZ	2B	AX-109390001	AX-110571221	0.00—1.92	7.41	22.40	0.10
		2019CZ	2B	AX-109390001	AX-110571221	0.00—1.92	6.19	21.77	0.09
		2018CZ	2B	AX-109390001	AX-110571221	0.00—1.92	6.50	29.41	0.11
		BLUP	2B	AX-109390001	AX-110571221	0.00—1.92	12.89	33.29	0.08
	QEW.sicau-AM-2A-1	2020WJ	2A	AX-109455008	AX-109024104	70.71—74.53	2.55	7.67	0.06
		2019CZ	2A	AX-109455008	AX-109024104	70.71—74.53	2.75	9.12	0.06
		BLUP	2A	AX-109455008	AX-109024104	70.71—74.53	4.80	10.74	0.05
	QEW.sicau-AM-2A-2	2020CZ	2A	AX-109934383	AX-109314401	98.92—105.82	3.31	9.79	0.07
胚长/粒长 EL/KL	QEL/KL.sicau-AM-1B-1	2020YA	1B	AX-109379021	AX-111720572	49.00—50.30	2.78	12.45	0.01
	QEL/KL.sicau-AM-1B-2	2020CZ	1B	AX-108913437	AX-111510846	46.84—47.27	3.07	12.68	0.01
	QEL/KL.sicau-AM-4B	2019CZ	4B	AX-109052054	AX-109931786	11.56—12.43	2.77	11.29	0.01
	QEL/KL.sicau-AM-5B	2019CZ	5B	AX-109990234	AX-108886889	76.90—79.56	2.54	10.13	-0.01
	QEL/KL.sicau-AM-2A	BLUP	2A	AX-109455008	AX-109024104	70.71—74.53	3.70	9.03	0.01
	QEL/KL.sicau-AM-2B	BLUP	2B	AX-109390001	AX-110571221	0.00—1.92	6.66	20.67	0.01
胚宽/粒宽 EW/KW	QEW/KW.sicau-AM-2B	2020CZ	2B	AX-109390001	AX-110571221	0.00—1.92	4.05	12.49	0.02
		2019CZ	2B	AX-109390001	AX-110571221	0.00—1.92	2.57	8.80	0.02
		2018CZ	2B	AX-109390001	AX-110571221	0.00—1.92	5.16	24.92	0.02
		BLUP	2B	AX-109390001	AX-110571221	0.00—1.92	6.65	20.68	0.01
	QEW/KW.sicau-AM-4A	2019CZ	4A	AX-109272723	AX-110524110	11.88—31.77	2.78	13.49	-0.02
	QEW/KW.sicau-AM-6B	2020CZ	6B	AX-111012548	AX-108984460	136.24—162.46	3.47	10.94	-0.02
胚长/胚宽 EL/EW	QEL/EW.sicau-AM-2B-1	2020WJ	2B	AX-109390001	AX-110571221	0.00—1.92	8.00	21.55	-0.08
		2020YA	2B	AX-109390001	AX-110571221	0.00—1.92	4.04	16.41	-0.09
		2020CZ	2B	AX-109390001	AX-110571221	0.00—1.92	8.83	25.69	-0.08
		2019CZ	2B	AX-109390001	AX-110571221	0.00—1.92	4.19	12.79	-0.06
		2018CZ	2B	AX-109390001	AX-110571221	0.00—1.92	5.45	23.41	-0.07
		BLUP	2B	AX-109390001	AX-110571221	0.00—1.92	11.64	31.13	-0.06
性状 Trait	数量性状位点 QTL	环境Environment	染色体 Chromosome	左标记 Left marker	右标记 Right marker	位置 Position (cM)	阈值 LOD	表型变异率 PVE (%)	加性效应 Add
	QEL/EW.sicau-AM-2B-2	2018CZ	2B	AX-111525915	AX-109297362	64.00—66.68	3.01	12.12	-0.05
	QEL/EW.sicau-AM-2B-3	2020WJ	2B	AX-110447950	AX-108942420	33.21—39.07	3.03	7.89	-0.05
	QEL/EW.sicau-AM-6B	2020CZ	6B	AX-111012548	AX-108984460	136.24—162.46	3.38	9.10	0.05
	QEL/EW.sicau-AM-2A-1	2020WJ	2A	AX-109314401	AX-110069674	105.82—124.56	3.14	8.05	-0.05
	QEL/EW.sicau-AM-2A-2	BLUP	2A	AX-110492886	AX-109523500	92.49—95.29	2.76	6.33	-0.03
胚面积 EA	QEA.sicau-AM-2B	2020WJ	2B	AX-109390001	AX-110571221	0.00—1.92	4.23	11.34	0.25
		2019CZ	2B	AX-109390001	AX-110571221	0.00—1.92	2.88	10.47	0.21
		2018CZ	2B	AX-109390001	AX-110571221	0.00—1.92	3.01	15.22	0.25
		BLUP	2B	AX-109390001	AX-110571221	0.00—1.92	6.66	20.67	0.01
	QEA.sicau-AM-2A-1	2020WJ	2A	AX-109437513	AX-109974497	66.15—67.06	4.29	11.41	0.25
	QEA.sicau-AM-2A-2	BLUP	2A	AX-109455008	AX-109024104	70.71—74.53	3.07	9.03	0.01
	QEA.sicau-AM-3B	2019CZ	3B	AX-110375013	AX-110569582	6.91—7.34	4.65	17.65	-0.28
	QEA.sicau-AM-4B	2020CZ	4B	AX-108737791	AX-110544397	20.75—22.08	3.47	13.18	0.23

表4

图2

图3

表5

表6

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性状 Trait	环境 Environment	2020WJ	2020YA	2020CZ	2019CZ	2018CZ	BLUP
胚长 EL	2020WJ	1
	2020YA	0.34**	1
	2020CZ	0.49**	0.38**	1
	2019CZ	0.37**	0.32**	0.48**	1
	2018CZ	0.35**	0.15**	0.39**	0.36**	1
	BLUP	0.69**	0.58**	0.68**	0.68**	0.65**	1
胚宽 EW	2020WJ	1
	2020YA	0.39**	1
	2020CZ	0.38**	0.38**	1
	2019CZ	0.43**	0.25**	0.47**	1
	2018CZ	0.36**	0.41	0.34**	0.26*	1
	BLUP	0.63**	0.66**	0.65**	0.60**	0.59**	1
胚长/粒长 EL/KL	2020WJ	1
	2020YA	0.06	1
	2020CZ	0.46**	0.19	1
	2019CZ	0.45**	0.08	0.43**	1
	2018CZ	0.37**	0.10	0.27*	0.31**	1
	BLUP	0.68**	0.39**	0.64**	0.62**	0.51**	1
胚宽/粒宽 EW/KW	2020WJ	1
	2020YA	0.29**	1
	2020CZ	0.36**	0.30**	1
	2019CZ	0.39**	0.35**	0.32**	1
	2018CZ	0.54**	0.35**	0.42**	0.32**	1
	BLUP	0.72**	0.69**	0.72**	0.68**	0.69**	1
胚长/胚宽 EL/EW	2020WJ	1
	2020YA	0.42**	1
	2020CZ	0.48**	0.61**	1
	2019CZ	0.46**	0.42**	0.56**	1
	2018CZ	0.63**	0.44**	0.43**	0.37**	1
	BLUP	0.78**	0.78**	0.73**	0.71**	0.73**	1
胚面积 EA	2020WJ	1
	2020YA	0.44**	1
	2020CZ	0.62**	0.37**	1
	2019CZ	0.58**	0.32**	0.48**	1
	2018CZ	0.51**	0.41**	0.56**	0.54**	1
	BLUP	0.80**	0.69**	0.79**	0.75**	0.75**	1

性状 Trait	QTL	遗传标记区间 Flanking markers	野生二粒小麦参考基因组 v2.0物理位置 Wild Emmer v2.0 physical map (Mb)	中国春参考基因组v2.1物理位置 Chines Spring wheat v2.1 physical map (Mb)
胚长 EL	QEL.sicau-AM-3B	AX-110375013—AX-110569582	695.42—696.83	690.52—692.06
胚长 EL	QEW.sicau-AM-2B	AX-109390001—AX-110571221	6.41—53.55	3.69—9.15
胚宽 EW	QEL/KL.sicau-AM-1B-1	AX-108913437—AX-111510846	409.17—411.07	395.61—397.54
胚宽 EW	QEL.sicau-AM-1B-2	AX-111720572—AX-110579523	432.54—447.52	418.79—434.50
胚长/粒长 EL/KL	QEW.sicau-AM-2A-1	AX-109455008—AX-109024104	614.32—618.45	615.12—619.25
胚宽/粒宽 EW/KW	QEW/KW.sicau-AM-2B	AX-109390001—AX-110571221	6.41—53.55	3.69—9.15
胚宽/粒宽 EW/KW	QEA.sicau-AM-2B	AX-109390001—AX-110571221	6.41—53.55	3.69—9.15
胚面积 EA	QEW/KW.sicau-AM-6B	AX-111012548—AX-108984460	712.45—723.67	721.64—730.61

性状 Trait	QTL	染色体Chromosome	左标记 Left marker		右标记 Right marker
性状 Trait	QTL	染色体Chromosome	标记ID Marker ID	物理位置 WE v2.0/CS 2.1 (Mb)	标记ID Marker ID	物理位置 WE v2.0/CS 2.1(Mb)
粒长Kernel length	qKL.3B	3B	*	*	Xwmc754	21.66/14.4
	QGl.ccsu-3B.1	3B	Xgwm566	91.51/77.7	Xgwm376	64.63/38.9
	QKL.sicau-AM-3B	3B	AX-110375013	695.42/690.52	AX-111112626	675.62/655.4
胚长EL	QEL.sicau-AM-3B	3B	AX-110375013	695.42/690.52	AX-110569582	696.83/692.06
胚宽EW	QEW.sicau-AM-2B	2B	AX-109390001	6.41/3.69	AX-110571221	53.55/9.15
千粒重 Thousand grain weight	QTgw.crc-2B	2B	Xwmc661	6.49/9.53	*	*
粒重Kernel weight	qKLW2B-1	2B	Xgwm501	98.15/680.09	Xmag3512	780.69/771.06
粒宽Kernel width	QKw.ncl-2B.1	2B	Xbarc183	123.83/2.60	Xbarc13	134.83/125.20
粒宽Kernel width	QTkw.ncl-2B.1	2B	Xbarc183	123.83/2.60	Xbarc7	134.82/125.20

四倍体小麦胚大小性状QTL定位与分析

Mapping and Analysis of QTL for Embryo Size-Related Traits in Tetraploid Wheat

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性状 Trait	分蘖数 Tiller number	株高 Plant height	粒长 Kernel length	粒宽 Kernel width	千粒重 Thousand-kernel weight	开花期 Flowering period
胚长 EL	0.03	0.62**	0.71**	0.33**	0.71**	-0.11
胚宽 EW	-0.06	0.16	0.02	0.36**	0.31**	0.08
胚长/粒长 EL/KL	-0.27**	0.08	-0.19*	0.36**	0.08	0.11
胚宽/粒宽 EW/KW	-0.12	-0.01	-0.07	-0.15	0.17	0.13
胚长/胚宽 EL/EW	0.07	0.33**	0.53**	-0.08	0.23*	-0.13
胚面积 EA	-0.02	0.42**	0.38**	0.40**	0.57**	-0.02

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