中国农业科学 ›› 2015, Vol. 48 ›› Issue (15): 2939-2951.doi: 10.3864/j.issn.0578-1752.2015.15.004

• 耕作栽培·生理生化·农业信息技术 • 上一篇    下一篇

施氮量对春谷农艺性状、光合特性和产量的影响

张艾英,郭二虎,王军,范惠萍,李瑜辉,王丽霞,王秀清,程丽萍   

  1. 山西省农业科学院谷子研究所/杂粮种质资源发掘与遗传改良山西省重点实验室,山西长治 046011
  • 收稿日期:2014-12-04 出版日期:2015-08-01 发布日期:2015-08-01
  • 通讯作者: 郭二虎,Tel:13994673899;E-mail:guoerhu2003@163.com;王军,Tel:13333550810;E-mail:128wan@163.com
  • 作者简介:张艾英,Tel:15534100338;E-mail:zay1012@126.com
  • 基金资助:
    国家现代农业产业体系建设专项(CARS-07-12.5-A10)

Effect of Nitrogen Application Rate on Agronomic, Photosynthetic Characteristics and Yield of Spring Foxtail Millet

ZHANG Ai-ying, GUO Er-hu, WANG Jun, FAN Hui-ping, LI Yu-hui, WANG Li-xia, WANG Xiu-qing, CHENG Li-ping   

  1. Millet Research Institute, Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops, Changzhi 046011, Shanxi
  • Received:2014-12-04 Online:2015-08-01 Published:2015-08-01

摘要: 【目的】通过分析不同氮素水平下春谷品种农艺性状、光合特性与产量的变化规律,确定春谷最佳施氮量,并探讨光合特性与产量相关性。【方法】以春谷品种长农35号和晋谷21号为试验材料,采用裂区设计,品种为主区、施氮量为副区,重复3次,小区面积15.0 m2(5 m×3 m),留苗30万株/hm2。共设0(N1)、45(N2)、90(N3)、135(N4)、180(N5)和 225 kg·hm-2(N6)6个氮素水平,40%氮肥作底施,60%在拔节至孕穗期追施。于谷子抽穗后,用日本产叶绿素测定仪SPAD-502(Konica Minolta)测定顶三叶SPAD值,用美国产CIRAS-2光合速率仪(PPSYSTEMS)测定谷子顶三叶的细胞间隙二氧化碳浓度(Ci)、光合速率(Pn)和蒸腾速率(E)。【结果】随着氮素水平的提高,春谷品种的株高、茎粗、穗长呈上升趋势,穗重、穗粒重、旗叶与顶三叶SPAD值、蒸腾速率(E)及光合速率(Pn)表现为先升高后降低,千粒重在各氮素处理间无显著差异,氮水平为90 kg·hm-2 时,以上各指标值(千粒重除外)达到或接近最大,产量趋于稳定,预示氮肥施用量90 kg·hm-2 为春谷最佳施氮量,进一步通过2个春谷品种产量随氮素施用量变化回归方程计算出最高理论产量,方差分析表明两品种最高理论产量和施氮90 kg·hm-2 时产量无显著差异(P长农35号=0.5571、P晋谷21号=0.6632)。综合以上结果,将90 kg·hm-2 施氮量确定为春谷最佳施氮量。春谷光合生理指标与产量相关性分析表明:谷子旗叶蒸腾速率(E)及光合速率(Pn)与产量相关系数分别为 0.87和0.86,顶三叶总蒸腾速率(E)及总光合速率(Pn)与产量相关系数分别为 0.82和0.83,4个相关系数值均达到显著水平。【结论】明确了春谷品种在山西中南部生态气候和土壤条件下的最佳施氮量为90 kg·hm-2,发现谷子开花后旗叶蒸腾速率(E)及光合速率(Pn)、顶三叶蒸腾速率(E)及光合速率(Pn)与春谷产量呈显著正相关。

关键词: 春谷, 施氮量, 光合特性, 产量, 相关分析

Abstract: 【Objective】The aim of the study is to determine the optimal amount of nitrogen application and to investigate the correlation between photosynthetic characteristics and yield of spring foxtail millet varieties through analyzing the performance of agronomic traits, photosynthetic characteristics and yield under different nitrogen levels.【Method】The experiment was carried out in a field-split plot design with three replicates with varieties as main plot and nitrogen fertilizer as secondary plot. Cultivars Changnong 35 and Jingu 21 were selected as the experimental materials, and the area of each plot was 15.0 m2(5 m×3 m) and the seedling density was 3×105 plants/hm2. The designed 6 levels of nitrogen were 0 kg·hm-2 (N1), 45 kg·hm-2 (N2), 90 kg·hm-2 (N3), 135 kg·hm-2 (N4), 180 kg·hm-2 (N5) and 225 kg·hm-2 (N6), respectively. Forty percent of the nitrogen was applied as base fertilizer, and 60% applied at jointing stage to booting stage. After plants heading, the SPAD (Soil and Plant Analyzer Development, SPAD) value of the top-three leaves was measured through SPAD-502 (Konica Minolta), and the Ci (intercellular CO2 concentration, Ci), Pn (net photosynthesis rate, Pn) and E (transpiration rates, E) of the top-three leaves were measured using CIRAS-2 (PPSYSTEMS). 【Result】As the nitrogen levels increased, plant height, stem diameter and panicle length of spring foxtail millet exhibited a rising trend, and weight per panicle, grains weight per panicle and SPAD, E and Pn values of the flag leaf and the top-three leaves increased first and then decreased, however, 1000-grain weight had no significant difference in various nitrogen treatments. All of the above traits excepting 1000-grain weight reached to or tended to the highest value, meanwhile the yield tended to be stable at N level of 90 kg·hm-2 for spring foxtail millet, which suggested that the N level of 90 kg·hm-2 may be as the optimal amount of nitrogen application for spring foxtail millet. Furthermore, a regression equation between yield and various N levels was established and the maximum theoretical yield of spring millet was calculated. The results showed that there was no significant difference between the maximum theoretical yield and the observed one at N level of 90 kg·hm-2 (PChangnong 35 = 0.5571, PJingu 21= 0.6632). Therefore, the N level of 90 kg·hm-2 was confirmed as the optimal amount of nitrogen application for spring foxtail millet. The correlation analysis showed that the correlation coefficients between E, Pn of the flag leaf and yield and E, Pn of the top-three leaves and yield were 0.87, 0.86, 0.82, 0.83, respectively, and all reached a significant level.【Conclusion】 The optimal amount of nitrogen application was 90 kg·hm-2 in South Central Shanxi ecological region and soil conditions, and E and Pn of the flag leaf and the top-three leaves were significantly positively correlated with the yield of spring foxtail millet.

Key words: spring foxtail millet, nitrogen application rate, photosynthetic characteristics, yield, correlation analysis