棉花营养物质和单宁含量与其对绿盲蝽抗性的关系

doi:10.3864/j.issn.0578-1752.2013.22.006

摘要/Abstract

摘要： 【目的】系统研究不同棉花品种对绿盲蝽的抗性水平，并在室内测定不同棉花品种不同生育期以及不同组织的蛋白质、可溶性糖和单宁物质的含量，旨在明确棉花体内这些物质与品种抗性的关系。【方法】以12个棉花品种为材料，通过连续2年田间抗性鉴定确定这些棉花品种的抗性水平；然后分别采用Bradford法、蒽酮-硫酸法和香草醛法通过室内试验测定不同棉花品种各生育期不同组织蛋白质、可溶性糖和单宁的含量；最后进行12个棉花品种蛋白质、可溶性糖和单宁含量与其对绿盲蝽抗性的相关性分析。【结果】不同棉花品种对绿盲蝽抗性水平存在明显差异，其中云南黄绒土棉和美中棉为高抗品种（综合抗性指数＜1），AH-510、苏联棉、荆7516-1和池州红叶棉为高感品种（综合抗性指数＞3）。棉蕾和棉铃中蛋白质含量随着棉花对绿盲蝽抗性的增强而降低；可溶性糖含量在苗期顶叶中随着棉花对绿盲蝽抗性的增强而增加，但在棉蕾中随着棉花对绿盲蝽抗性的增强而降低；单宁含量在棉蕾中随着棉花对绿盲蝽抗性的增强而降低，但在铃期顶叶中随着棉花对绿盲蝽抗性的增强而增加。【结论】12个棉花品种中云南黄绒土棉对绿盲蝽抗性最强，池州红叶棉对绿盲蝽抗性最弱。蕾期棉蕾及铃期棉铃中蛋白质含量与其对绿盲蝽抗性存在显著负相关；苗期顶叶中可溶性糖含量和其对绿盲蝽抗性呈显著正相关，而蕾期棉蕾中可溶性糖含量和其对绿盲蝽抗性呈显著负相关；蕾期棉蕾中单宁含量和其对绿盲蝽抗性呈显著负相关，而铃期顶叶中单宁含量和其对绿盲蝽抗性呈显著正相关。

关键词: 棉花 , 营养物质 , 单宁 , 绿盲蝽 , 抗虫性

Abstract: 【Objective】 The objective of this study is to clarify the relationship between the contents of protein, soluble sugar and tannin in different tissues along developmental stage of various cotton varieties and their resistance to Apolygus lucorum.【Method】 By using the 12 cotton varieties as materials, with the method of resistance appraising in the field for two consecutive years and with the Bradford method, Anthrone-sulfuric acid method and Vanillin method, tests were made in laboratory to make clear the relationship between the contents of protein, soluble sugar and tannin in different tissues along developmental stage of various cotton varieties and their resistance to A. lucorum.【Result】The resistant level of different cotton varieties was obviously different, Yunnanhuangrongtumian and Meizhongmian were high resistant varieties (integrate resistance index＜1), AH-510, Sulianmian, Jing 7516-1 and Chizhou red leaf were low resistant varieties (integrate resistance index＞3). The content of protein in bud in budding period and boll of bolling period decreased with the increase of resistance of the cotton varieties to A. lucorum. The content of soluble sugar in leaf in seeding period increased with the increase of resistance of the cotton varieties to A. lucorum, but in bud in budding period decreased with the increase of resistance of the cotton varieties to A. lucorum. The content of tannin in bud in budding period decreased with the increase of resistance of the cotton varieties to A. lucorum, but in boll in bolling period increased with the increase of resistance of the varieties to A. lucorum. 【Conclusion】Yunnanhuangrongtumian had the highest resistance levels to A. lucorum, and Chizhou red leaf had the lowest resistance levels to A. lucorum. The content of protein in bud in budding period and boll in bolling period indicated a clear negative linear correlation with the resistance to A. lucorum. The content of soluble sugar in leaf in seeding period indicated a clear positive linear correlation with the resistance to A. lucorum, but in bud in budding period indicated a clear negative linear correlation with the resistance to A. lucorum. The content of tannin in bud in budding period indicated a clear negative linear correlation with the resistance to A. lucorum, but in boll in bolling period indicated a clear positive linear correlation with the resistance to A. lucorum.

Key words: cotton , nutrients , tannin , Apolygus lucorum Meyer-Dür , resistance to pests

杨宇晖, 张青文, 刘小侠. 棉花营养物质和单宁含量与其对绿盲蝽抗性的关系[J]. 中国农业科学, 2013, 46(22): 4688-4697.

YANG Yu-Hui, ZHANG Qing-Wen, LIU Xiao-Xia. The Relationship Between the Contents of Nutrients and Tannins in Different Cotton Varieties and Their Resistance to Apolygus lucorum[J]. Scientia Agricultura Sinica, 2013, 46(22): 4688-4697.

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