双去甲氧基姜黄素及其N-甲基吡唑衍生物对朱砂叶螨 生物活性和多种酶活性的影响

doi:10.3864/j.issn.0578-1752.2013.13.020

摘要/Abstract

摘要： 【目的】明确双去甲氧基姜黄素（bisdemethoxycurcumin，BDMC）及其N-甲基吡唑衍生物（N-methylpyrazolebisdemethoxycurcumin，N-MPBDMC）对朱砂叶螨（Tetranychus cinnabarinus Boisduval）的生物活性及其体内多种酶活性的影响，为进一步证实其作用机理提供依据。【方法】采用玻片浸渍法测定BDMC及N-MPBDMC对朱砂叶螨雌成螨的触杀毒力，观察两者对朱砂叶螨的致毒症状，并测定其对朱砂叶螨乙酰胆碱酯酶（AchE）、Ca2+-ATP酶（Ca2+-ATPase）、单胺氧化酶（MAO）、谷胱甘肽S-转移酶（GSTs）和羧酸酯酶（CarE）活性的影响。【结果】N-MPBDMC对朱砂叶螨雌成螨的触杀活性优于先导化合物BDMC，其中处理72 h后，N-MPBDMC对朱砂叶螨的LC50为21.77 mg•L-1，其毒力是BDMC的10.03倍，与95%哒螨灵原药的毒力相当。BDMC和N-MPBDMC分别处理朱砂叶螨雌成螨后，中毒试螨表现出类似神经毒剂的致毒症状，螨体内GSTs和CarE活性均有不同程度上升，AchE、Ca2+-ATPase和MAO活性均有不同程度下降，其中AchE活性平均分别下降了16.77%和36.57%，Ca2+-ATPase活性平均分别下降了17.55%和17.45%，MAO活性平均分别下降了8.04%和14.19%，GSTs活性平均分别上升了17.13%和18.93%，CarE活性平均分别上升了26.78%和25.98%。【结论】BDMC和N-MPBDMC对朱砂叶螨的致死作用可能与其显著降低螨体内神经系统相关酶活性有关，具体的作用靶标还有待进一步确证。

关键词: 双去甲氧基姜黄素 , 双去甲氧基姜黄素N-甲基吡唑 , 朱砂叶螨 , 酶活性

Abstract: 【Objective】The objectives of this study are to investigate the acaricidal activity of bisdemethoxycurcumin (BDMC) and N-methylpyrazolebisdemethoxycurcumin (N-MPBDMC) against Tetranychus cinnabarinus (Boisduval) and their effects on enzymes activity in the mite and to provide a basis for the further elucidation acaricidal mechanism of these two compounds.【Method】The acaricidal activity of BDMC and N-MPBDMC against female adults of T. cinnabarinus was tested using the slide dip method and the poisoning symptoms of BDMC and N-MPBDMC exposed mites were carefully observed. The activities of acetylcholinesterase (AchE), Ca2+-ATP enzyme (Ca2+-ATPase), monoamine oxidase enzyme (MAO), glutathione S-transferases (GSTs) and carboxylesterase (CarE) of T. cinnabarinus treated with these two compounds were also determined.【Result】The results of acaricidal bioassay indicated that N-MPBDMC exhibited more pronounced acaricidal activity than BDMC against female adults of T. cinnabarinus. After 72 h of T. cinnabrinus treatment, the LC50 of N-MPBDMC was 21.77 mg•L-1, the acaricidal potency was about 10.03-fold higher than BDMC and comparable with that of 95% pyridaben TC. Some similar neurotoxic symptoms were observed in BDMC and N-MPBDMC-exposed mites. After T. cinnabrinus treatment with BDMC and N-MPBDMC, respectively, the activities of GSTs and CarE were strongly rising whereas the activities of AchE, Ca2+-ATPase and MAO were declining, the average activities of AchE decreased by 16.77% and 36.57%, that of Ca2+-ATPase decreased by 17.55% and 17.45%, that of MAO decreased by 8.04% and 14.19%, while that of GSTs increased by 17.13% and 18.93%, and that of CarE increased by 26.78% and 25.98%, respectively.【Conclusion】The mortality of mites that caused by BDMC and N-MPBDMC related to the decline of neural system related enzyme activities, and the specific action target remains to be further confirmed.

Key words: bisdemethoxycurcumin , N-methylpyrazolebisdemethoxycurcumin , Tetranychus cinnabarinus , enzymes activity

罗金香, 丁伟, 张永强, 杨振国, 李阳, 丁丽娟. 双去甲氧基姜黄素及其N-甲基吡唑衍生物对朱砂叶螨生物活性和多种酶活性的影响[J]. 中国农业科学, 2013, 46(13): 2833-2844.

LUO Jin-Xiang, DING Wei, ZHANG Yong-Qiang, YANG Zhen-Guo, LI Yang, DING Li-Juan. Acaricidal Activity of Bisdemethoxycurcumin and N-methylpyrazolebisdemethoxycurcumin Against Tetranychus cinnabarinus (Boisduval) and Their Effects on Enzymes Activity in the Mite[J]. Scientia Agricultura Sinica, 2013, 46(13): 2833-2844.

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