关键词: 红麻, 干旱胁迫, 差异蛋白, 双向电泳

Abstract: 【Objective】 The analysis of stress-responsiveness in kenaf is an important route to the discovery of genes conferring stress tolerance. Proteomic analysis provides a broad view of plant responses to stress at the level of proteins.【Method】A drought-resistant kenaf variety GA42 has been identified as materials by setting the normal water supply and water control comparison test at seeding stage, stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis.【Result】In total, 9 up-regulated protein spots reproducibly presented differential expression patterns on 2-DE maps. Six spots were identified and confirmed by MALDI-TOF-TOF MS, including 2 ribulose-1, 5-bisphosphate carboxylase or large chain (a key enzyme in the first major step of carbon fixation), 1 rubisco activase (a enzyme could regulate the activity of rubisco existing widely in plants), 1 dimethylmenaquinone methyltransferase (a coenzyme participates in methyl transfer reactions), 1 putative plastidic glutamine synthetase (a key enzyme participates in ammonia assimilation in higher plants), and 1 ATP synthase subunit β (an important enzyme that provides energy for the cell to use through the synthesis of ATP, which is the most commonly used “energy currency” of the cells from most organisms). 【Conclusion】 This study preliminarily showed these six protein spots are up-regulated which are associated with GA42 resistance under drought stress.

Key words: kenaf (Hibiscus cannabinus L.), drought stress, differential protein, 2-DE