远红光补光对辣椒幼苗生长和非生物胁迫抗性的影响

doi:10.3864/j.issn.0578-1752.2022.06.011

摘要/Abstract

摘要：

【目的】研究补照适量远红光（FR）对辣椒幼苗生长发育和非生物胁迫抗性的调控作用,旨在为实际生产过程中利用精确的光环境调控手段培育壮苗提供理论依据。【方法】以辣椒‘博辣红帅’品种为研究材料,将苗龄7 d的辣椒幼苗置于LED光源对照光谱（NL;红R/蓝B=3/1,光量子通量密度PPFD为150 μmol·m^-2·s^-1）及在此基础上分别补充10 μmol·m ^-2·s^-1远红光（6% FR）、20 μmol·m ^-2·s^-1远红光（13% FR）和30 μmol·m ^-2·s^-1远红光（20% FR）的处理组光谱环境条件下培养,并于苗龄21 d时进行低温和干旱处理。通过测定生物量、抗性相关基因表达、抗氧化酶活性、激素含量、叶绿素荧光参数以及叶片相对电导率等,探究补充6% FR对辣椒幼苗生长和非生物胁迫抗性的影响。【结果】与对照光谱相比,补充6% FR显著提高了辣椒幼苗株高、茎粗、干鲜重以及壮苗指数（P<0.05）。低温胁迫下,相比于对照光谱组,补充6% FR显著提高了辣椒叶片冷响应基因CBF1和抗氧化酶相关基因Cu/Zn-SOD、GR、APX、CAT、DHAR的表达水平,超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）活性比对照分别增加25.2%、53.6%、55.8%、72.7%和33.4%,抗逆相关激素脱落酸（ABA）的含量提高69.5%。同时,低温胁迫下补充6% FR后辣椒叶片PSII最大光化学效率（Fv/Fm）较对照光谱组显著升高,而相对电导率（REL）显著降低,表明补充6% FR缓解了低温下PSII光抑制和叶片细胞的损伤,提高了辣椒幼苗的耐冷性。此外,干旱胁迫下,相比于对照光谱组,补充6% FR使辣椒幼苗的抗氧化酶SOD、GR、APX、CAT、DHAR活性分别增加13.7%、38.0%、37.2%、27.6%和23.7%,ABA含量和PSII实际光化学效率（Φ_PSII）也显著升高,而REL则明显降低,表明补充6% FR减轻了干旱胁迫引起的PSII光抑制和膜脂过氧化,提高了辣椒幼苗的耐旱性。【结论】补充6% FR不仅可促使辣椒壮苗的形成,还可通过增加抗氧化酶活性和ABA含量提高辣椒幼苗对低温胁迫和干旱胁迫的抗性。

关键词: 辣椒, 远红光, 壮苗指数, 低温胁迫, 干旱胁迫

Abstract:

【Objective】This study analyzed the effects of supplementary far-red light (FR) on the growth and abiotic stress tolerance of pepper seedlings, aiming to provide a theoretical basis on precise light environments for cultivating high quality vegetable seedlings.【Method】In this study, Bola Hongshuai pepper cultivar was used as the research material. The 7-day-old seedlings were cultivated under two LED light environments, including the control spectrum (NL; R/B = 3/1, 150 μmol∙m ^-2∙s^-1 PPFD) and the NL with an extra 10 μmol∙m ^-2∙s^-1far-red light (6% FR), 20 μmol∙m ^-2∙s^-1far-red light (13% FR), and 30 μmol∙m ^-2∙s^-1far-red light (20% FR). Chilling and drought were imposed when the seedlings were 21 days old. Biomass, resistance-related gene expression, antioxidant enzyme activity, hormone content, chlorophyll fluorescence parameters, and leaf relative electrolyte leakage (REL) were analyzed to explore the effects of supplemental FR on growth and abiotic stress tolerance of pepper seedlings. 【Result】 Compared with the control, the supplementation of 6% FR was beneficial to increase the height, stem thickness, dry weight, fresh weight and seedling indexes of pepper seedlings. Moreover, the supplementation of 6% FR significantly increased the expression of the cold response gene CBF1 and antioxidant enzyme-related genes, such as Cu/Zn-SOD, GR, APX, CAT and DHAR under chilling stress. The activity of SOD, APX, DHAR, CAT and GR as well as the ABA content of pepper seedlings under low temperature increased by 25.2%, 53.6%, 55.8%, 72.7%, 33.4% and 69.5%, respectively, following the treatment with supplemental FR. The PSII maximum photochemical efficiency (Fv/Fm) of pepper leaves after supplementation of 6% FR under low temperature stress significantly increased compared with the control, while REL decreased obviously, indicating that supplementation of 6% FR alleviated the low temperature-induced PSII photoinhibition and damage in leaves and enhanced the cold tolerance of pepper seedlings. Under the drought stress condition, compared with the control, the supplementation of 6% FR improved the activity of SOD, GR, APX, CAT and DHAR by 13.7%, 38.0%, 37.2%, 27.6% and 23.7%, respectively. The ABA content and PSII actual photochemical efficiency (Φ_PSII) were both increased while REL was decreased significantly. The results showed that the supplementation of 6% FR reduced the degree of PSII photoinhibition and membrane lipid peroxidation caused by drought stress, and improved the drought tolerance of pepper seedlings.【Conclusion】The study found that supplementation of 6% FR could not only improve the quality of pepper seedlings, but also enhance the resistance of pepper seedlings to low temperature and drought stresses by improving antioxidant defense and ABA homeostasis.

Key words: pepper (Capsicum annuum L.), far-red light, seedling index, low temperature stress, drought stress

董桑婕,姜小春,王羚羽,林锐,齐振宇,喻景权,周艳虹. 远红光补光对辣椒幼苗生长和非生物胁迫抗性的影响[J]. 中国农业科学, 2022, 55(6): 1189-1198.

DONG SangJie,JIANG XiaoChun,WANG LingYu,LIN Rui,QI ZhenYu,YU JingQuan,ZHOU YanHong. Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings[J]. Scientia Agricultura Sinica, 2022, 55(6): 1189-1198.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2022.06.011

https://www.chinaagrisci.com/CN/Y2022/V55/I6/1189

图/表 9

图1

表1

图2

表2

图3

图4

图5

图6

图7

参考文献 32

[1]	JIAO Y L, LAU O S, DENG X W. Light-regulated transcriptional networks in higher plants. Nature Reviews Genetics, 2007,8(3):217-230. doi: 10.1038/nrg2049. doi: 10.1038/nrg2049
[2]	YAVARI N, TRIPATHI R, WU B S, MACPHERSON S, SINGH J, LEFSRUD M. The effect of light quality on plant physiology, photosynthetic, and stress response in Arabidopsis thaliana leaves. PLoS ONE, 2021,16(3):e0247380. doi: 10.1371/journal.pone.0247380. doi: 10.1371/journal.pone.0247380
[3]	MUNEER S, KIM E J, PARK J S, LEE J H. Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.). International Journal of Molecular Sciences, 2014,15(3):4657-4670. doi: 10.3390/ijms15034657
[4]	JOSHI J, ZHANG G, SHEN S Q, SUPAIBULWATANA K, WATANABE C K A, YAMORI W. A combination of downward lighting and supplemental upward lighting improves plant growth in a closed plant factory with artificial lighting. Hortscience, 2017,52(6):831-835. doi: 10.21273/HORTSCI11822-17
[5]	KWON S Y, RYU S H, LIM J H. Design and implementation of an integrated management system in a plant factory to save energy. Cluster Computing, 2014,17(3):727-740. doi: 10.1007/s10586-013-0295-2. doi: 10.1007/s10586-013-0295-2
[6]	ZHENG L, HE H M, SONG W T. Application of light-emitting diodes and the effect of light quality on horticultural crops: A Review. Hortscience, 2019,54(10):1656-1661. doi: 10.21273/HORTSCI14109-19
[7]	WU B S, HITTI Y, MACPHERSON S, ORSAT V, LEFSRUD M G. Comparison and perspective of converntional and LED lighting for photobiology and industry applications. Environmental and Experimental Botany, 2020,171:103953. doi: 10.1016/j.envexpbot.2019.103953
[8]	PRATT L H, CORDONNIERPRATT M M, KELMENSON P M, LAZAROVA G I, KUBOTA T, ALBA R M. The phytochrome gene family in tomato (Solanum lycopersicum L.). Plant Cell and Environment, 1997,20(6):672-677. doi: 10.1046/j.1365-3040.1997.d01-119.x
[9]	JUAN I C, EDMUNDO P, TOMÁS B A, SCOTT A. F, JORGE J C. Stem transcriptome reveals mechanisms to reduce the energetic cost of shade-avoidance responses in tomato. Plant Physiology, 2012,160(2):1110-1119. doi: 10.1104/pp.112.201921
[10]	DIEGO A M, JAVIER F B. Manipulation of light environment to produce high-quality poinsettia plants. Hortscience, 2009,44(3):702-706. doi: 10.21273/HORTSCI.44.3.702
[11]	KUREPIN L V, EMERY R J N, PHARIS R P, REID D M. Uncoupling light quality from light irradiance effects in Helianthus annuus shoots: putative roles for plant hormones in leaf and internode growth. Journal of Experimental Botany, 2007,58(8):2145-2157. doi: 10.1093/jxb/erm068. doi: 10.1093/jxb/erm068
[12]	杨有新, 王峰, 蔡加星, 喻景权, 周艳虹. 光质和光敏色素在植物逆境响应中的作用研究进展. 园艺学报, 2014,41(9):1861-1872.
	YANG Y X, WANG F, CAI J X, YU J Q, ZHOU Y H. Recent advances in the role of light quality and phytochrome in plant defense resistance against environmental stresses. Acta Horticulturae Sinica, 2014,41(9):1861-1872. (in Chinese)
[13]	张晓梅, 胡超轶, 刘涛, 周艳虹. 不同光质对黄瓜幼苗抗旱性的影响. 浙江农业学报, 2017,29(1):58-63. doi: 10.3969/j.issn.1004-1524.2017.01.09. doi: 10.3969/j.issn.1004-1524.2017.01.09
	ZHANG X M, HU C Y, LIU T, ZHOU Y H. Effects of light quality on drought resistance of cucumber seedlings. Acta Agriculturae Zhejiangensis, 2017,29(1):58-63. doi: 10.3969/j.issn.1004-1524.2017.01.09. (in Chinese) doi: 10.3969/j.issn.1004-1524.2017.01.09
[14]	张云婷, 宋霞, 叶云天, 冯琛, 孙勃, 王小蓉, 汤浩茹. 光质对低温胁迫下草莓叶片生理生化特性的影响. 浙江农业学报, 2016,28(5):790-796. doi: 10.3969/j.issn.1004-1524.2016.05.13. doi: 10.3969/j.issn.1004-1524.2016.05.13
	ZHANG Y T, SONG X, YE Y T, FENG C, SUN B, WANG X R, TANG H R. Effects of light quality on physiological and biochemical indexes in strawberry leaves under low temperature stress. Acta Agriculturae Zhejiangensis, 2016,28(5):790-796. doi: 10.3969/j.issn.1004-1524.2016.05.13. (in Chinese) doi: 10.3969/j.issn.1004-1524.2016.05.13
[15]	WANG F, GUO Z X, LI H Z, WANG M M, ONAC E, ZHOU J, XIA X J, SHI K, YU J Q, ZHOU Y H. Phytochrome A and B function antagonistically to regulate cold tolerance via abscisic acid-dependent jasmonate signaling. Plant Physiology, 2015,170(1):459-471. doi: 10.1104/pp.15.01171. doi: 10.1104/pp.15.01171
[16]	刘晓英, 常涛涛, 郭世荣, 徐志刚, 陈文昊. 红蓝LED光全生育期照射对樱桃番茄果实品质的影响. 中国蔬菜, 2010(22):21-27.
	LIU X Y, CHANG T T, GUO S R, XU Z G, CHEN W H. Effect of irradiation with blue and red LED on fruit quality of cherry tomato during growth period. China Vegetables, 2010(22):21-27. (in Chinese)
[17]	LI Q, KUBOTA C. Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Environmental and Experimental Botany, 2009,67(1):59-64. doi: 10.1016/j.envexpbot.2009.06.011
[18]	JIANG X C, XU J, LIN R, SONG J N, SHAO S J, YU J Q, ZHOU Y H. Light-induced HY5 functions as a systemic signal to coordinate the photoprotective response to light fluctuation. Plant Physiology, 2020,184(2):1181-1193. doi: 10.1104/pp.20.00294. doi: 10.1104/pp.20.00294
[19]	ZHANG L, JIANG X, LIU Q, AHAMMED G J, LIN R, WANG L, SHAO S, YU J, ZHOU Y. The HY5 and MYB15 transcription factors positively regulate cold tolerance in tomato via the CBF pathway. Plant, Cell & Environment, 2020,43(11):2712-2726. doi: 10.1111/pce.13868. doi: 10.1111/pce.13868
[20]	XIA X J, HUANG L F, ZHOU Y H, MAO W H, SHI K, WU J X, ASAMI T, CHEN Z X, YU J Q. Brassinosteroids promote photosynthesis and growth by enhancing activation of Rubisco and expression of photosynthetic genes in Cucumis sativus. Planta, 2009,230(6):1185-1196. doi: 10.1007/s00425-009-1016-1. doi: 10.1007/s00425-009-1016-1
[21]	王峰. PhyA、HY5和PIF4在光质调控番茄低温抗性中的机制研究[D]. 杭州: 浙江大学, 2017.
	WANG F. Roles and mechanisms of PhyA-, HY5-, and PIF4- mediated light quality-regulated cold tolerance in tomato [D]. Hangzhou: Zhejiang University. 2017. (in Chinese)
[22]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT Method. Methods (San Diego, Calif), 2001,25(4):402-408. doi: 10.1006/meth.2001.1262. doi: 10.1006/meth.2001.1262
[23]	PARK S, LEE C M, DOHERTY C J, GILMOUR S J, KIM Y, THOMASHOW M F. Regulation of the Arabidopsis CBF regulon by a complex low-temperature regulatory network. The Plant Journal, 2015,82(2):193-207. doi: 10.1111/tpj.12796. doi: 10.1111/tpj.12796
[24]	HANYU H, SHOJI K. Combined effects of blue light and supplemental far-red light and effects of increasing red light with constant far-red light on growth of kidney bean under mixtures of narrow-band light sources. Environment Control in Biology, 2000,38:25-32. doi: 10.2525/ecb1963.38.25
[25]	DEMOTES M S, PÉRON T, COROT A, BERTHELOOT J, GOURRIEREC J L, PELLESCHI T S, CRESPEL L, MOREL P, HUCHÉ T L, BOUMAZA R, VIAN A, GUÉRIN V, LEDUC N, SAKR S. Plant responses to red and far-red lights, applications in horticulture. Environmental and Experimental Botany, 2016,121:4-21. doi: 10.1016/j.envexpbot.2015.05.010
[26]	PARK Y, RUNKLE E S. Far-red radiation promotes growth of seedlings by increasing leaf expansion and whole-plant net assimilation. Environmental and Experimental Botany, 2017,136:41-49. doi: 10.1016/j.envexpbot.2016.12.013
[27]	KUREPIN L V, JOO S H, KIM S K, PHARIS R P, BACK T G. Interaction of brassinosteroids with light quality and plant hormones in regulating shoot growth of young sunflower and Arabidopsis seedlings. Journal of Plant Growth Regulation, 2012,31(2):156-164. doi: 10.1007/s00344-011-9227-7. doi: 10.1007/s00344-011-9227-7
[28]	CASAL J J. Photoreceptor signaling networks in plant responses to shade. Annual Review of Plant Biology, 2013,64:403-427. doi: 10.1146/annurev-arplant-050312-120221. doi: 10.1146/annurev-arplant-050312-120221
[29]	杨再强, 张继波, 李永秀, 彭晓丹, 张婷华, 张静. 红光与远红光比值对温室切花菊形态指标、叶面积及干物质分配的影响. 生态学报, 2012,32(8):2498-2505. doi: 10.5846/stxb
	YANG Z Q, ZHANG J B, LI Y X, PENG X D, ZHANG T H, ZHANG J. Effects of red/far-red ratio on morphological index, leaf area and dry matter partitioning of cut chrysanthemum flower. Acta Ecologica Sinica, 2012,32(8):2498-2505. (in Chinese) doi: 10.5846/stxb
[30]	彭晓丹, 杨再强, 李伶俐, 张继波. 红光与远红光比值对温室切花菊花‘神马’花芽分化进程的影响. 生态学杂志, 2013,32(6):1471-1475.
	PENG X D, YANG Z Q, LI L L, ZHANG J B. Effects of red and far-red light ratio on the flower bud differentiation of greenhouse cut chrysanthemum cultivar ‘Jingba’. Chinese Journal of Ecology, 2013,32(6):1471-1475. (in Chinese)
[31]	OUEDRAOGO M, HUBAC C. Effect of far red light on drought resistance of cotton. Plant and Cell Physiology, 1982,23(7):1297-1303. doi: 10.1093/oxfordjournals.pcp.a076474. doi: 10.1093/oxfordjournals.pcp.a076474
[32]	ZHANG C, XIE Q G, ANDERSON R G, NG G, SEITZ N C, PETERSON T, MCCLUNG C R, MCDOWELL J M, KONG D D, KWAK J M, LU H, AUSUBEL F M. Crosstalk between the circadian clock and innate immunity in Arabidopsis. PLOS Pathogens, 2013,9(6):e1003370. doi: 10.1371/journal.ppat.1003370

基因名称 Gene name	正向引物（5^′-3^′）Forward primer	反向引物（5^′-3^′）Reverse primer
UBI-3	TGTCCATCTGCTCTCTGTTG	CACCCCAAGCACAATAAGAC
CBF1	CCGGCTTCCTCTGACACTAA	GTAATCCCGGCATGCAGAAG
Cu/Zu-SOD	ATGGAGATGCTCCAACCACA	CCAAGATCACCAGCATGACG
APX	TCTGTGAAGCGGTGAAGTCT	GTGGCACACCTTGTTTAGCA
CAT	AAGCAGGCTGGGGAGAGATA	CATGAGTGACTCGGGGATCG
GR	CTTGTTGATGCGCAGACAGA	CAAACTGCTCCTTGGTTGCT
DHAR	GAGGTGAACCCAGAAGGGAA	CCCACAGAGGCAAATTCAGG

处理 Treatment	株高 Plant height (cm)	茎粗 Stem thickness (mm)	第一节间距 First internode length (cm)	下胚轴长度 Hypocotyl length (cm)	叶片数 Leaf number	地上部鲜重 Aboveground FW (g)	地下部鲜重 Underground FW (g)	总鲜重 Total FW (g)	地上部干重 Aboveground DW (g)	地下部干重 Underground DW (g)	总干重 Total DW (g)	壮苗指数 Seedling index
自然光NL	9.88d	2.13bc	3.45c	3.10c	5.83a	1.72b	0.52b	2.21b	0.186bc	0.053bc	0.246b	0.118b
6% FR	11.37c	2.53a	3.33c	3.13c	6.00a	2.25a	0.65a	2.79a	0.223a	0.076a	0.297a	0.167a
13% FR	13.85b	2.28b	3.90b	3.40b	5.67a	1.72b	0.43c	2.19b	0.192b	0.061b	0.238b	0.110bc
20% FR	15.31a	1.96c	4.31a	3.90a	5.50a	1.65b	0.41c	0.173c	0.361b	0.047c	0.224b	0.096c