中国桃主要品种资源及其野生近缘种的分子身份证构建

doi:10.3864/j.issn.0578-1752.2011.10.013

摘要/Abstract

摘要： 【目的】以国家果树种质郑州葡萄、桃圃保存的237份中国桃地方品种、育成品种及其野生近缘种为试材，进行种质分子身份证构建工作，并对构建方法进行探讨。【方法】采用筛选后的SSR标记对品种进行区分，然后根据引物对不同品种扩增条带分子量的大小进行编码、组合。【结果】从80对引物中筛选出来自桃8条染色体上的16对SSR引物，在供试种质间共检测出等位基因203个，每对引物平均检测到等位基因数为12.7个。根据等位基因既位于地方品种、育成品种，又位于近缘野生种的选择方法，筛选出123对等位基因并赋值后可用于构建种质的分子身份证。【结论】在237份种质中有202份具有的可辨分子身份证编码，继续对不同引物组合对种质的筛选效率进行分析，筛选出8对核心引物使176份种质具有可辨的分子身份证，平均每对引物区分种质达22.1份，即在保证每对引物区分效率较高的基础上，兼顾了总的区分数量。最后，为使区分品种简便化，依据引物的多样性指数先后选择引物组合，达到用最少的引物组合区分不同品种的目的。

关键词: 桃, 近缘种, SSR, 分子身份证

Abstract: 【Objective】 A total of 237 peach germplasms including China landraces, bred varieties, and related species selected from national grape and peach repository of Zhengzhou were studied with SSR(simple sequence repeat) markers. Analysis was made to establish the 237 peach germplasms molecular ID and to study the methods for distinguishing the germplasms more simply.【Method】Germplasms were distinguished with selected SSR markers, bands that amplified by each marker were coded, then combined the code as a molecular ID. 【Result】The results showed that after screening of the 80 primers, 203 alleles were detected using 16 selected SSR markers located on each chromosome of peach, with a mean value of 12.7 alleles/locus. There were 123 selected alleles were coded to establish germplasms molecular ID based on strategy that alleles located both in landraces, bred and related germplasms. 【Conclusion】There were 202 out of 237 germplasms could be identified with an particular combined code numbers. Moreover, the identifying efficiency of different combinations of the primers were analyzed, 176 germplasms could be identified using 8 reduced core primers, with an average value of 22.1 germplasms/primers. The remaining primers have a higher identifying efficiency and more germplasms could be identified separately. At last, for distinguish germplasms more simply, through selection of the primer pairs according to its PIC step by step, the germplasms could be distinguished by a practical primer choice.

Key words: peaches (Prunus persica (L.) Batsch), related species, SSR, molecular ID

陈昌文, 曹珂, 王力荣, 朱更瑞, 方伟超. 中国桃主要品种资源及其野生近缘种的分子身份证构建[J]. 中国农业科学, 2011, 44(10): 2081-2093.

CHEN Chang-Wen, CAO Ke, WANG Li-Rong, ZHU Geng-Rui, FANG Wei-Chao. Molecular ID Establishment of Main China Peach Varieties and Peach Related Species[J]. Scientia Agricultura Sinica, 2011, 44(10): 2081-2093.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.10.013

https://www.chinaagrisci.com/CN/Y2011/V44/I10/2081

参考文献

[1]FAO. Peaches and nectarines statistics, 2008. http://faostat.fao.org/ site/567/DesktopDefault.aspx?PageID=567#ancor.

[2]Association for Agriculture and Culture. Encyclopedia of Fruits-Prunus Persica. Tokyo: Agriculture Mountain and Fish Village Association in Japan, 1985: 83-91.

[3]Okie W R. Handbook of Peach and Nectarine Varieties. Springfield: The National Technical Information Service, 1998.

[4]汪祖华, 庄恩及. 中国果树志——桃卷. 北京: 中国林业出版社, 2001.

Wang Z H, Zhuang E J. Encyclopedia of Fruit in China—Scroll of Peach. Beijing: China Forestry Press, 2001. (in Chinese)

[5]Byrne D H. Trends in stone fruit cultivar development. Hort Technology, 2005(3): 494-500.

[6]宋婉, 续九如. 果树种质资源鉴定及DNA指纹图谱应用研究进展. 北京林业大学学报，2000, 22(1): 76-80.

Song W, Xu J R. Progress in application of germplasm identification and fingerprinting of fruit trees. Journal of Beijing Forestry University, 2000, 22(1): 76-80. (in Chinese)

[7]Morgante M, Olivieri A. PCR-amplified microsatellites as markers in plant genetics. The Plant Journal, 1993, 3(1): 175-182.

[8]Gabriela R, Marco A M, Carlos M, Gamalier L, Patricio H. Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars. Electronic Journal of Biotechnology, 2008, 11(5): 1-12.

[9]Aranzana M J, Carbó J, Arús P. Microsatellite variability in peach [Prunus persica (L.) Batsch]: Cultivar identification; marker mutation; pedigree inferences and population structure. Theoretical and Applied Genetics, 2003, 106(8): 1341-1352.

[10]Poljuha D, Sladonja B, Šeti? E, Miloti? A, Bandelj D, Jakše J, Javornik B. DNA fingerprinting of olive varieties in Istria (Croatia) by microsatellite markers. Scientia Horticulturae, 2008, 115(3): 223-230.

[11]Dangl G S, Yang J, Golino D A, Gradziel T. A practical method for almond cultivar identification and parental analysis using simple sequence repeat markers. Euphytica, 2009, 168: 41-48.

[12]艾呈祥, 张力思, 魏海蓉, 苑克俊, 金松南, 刘庆忠. 甜樱桃品种SSR指纹图谱数据库的建立. 中国农学通报, 2007, 23(5): 55-58.

Ai C X, Zhang L S, Wei H R, Yuan K J, Jin S N, Liu Q Z. Construction of molecular fingerprinting database for sweet cherry using SSR markers. Chinese Agricultural Science Bulletin, 2007, 23(5): 55-58. (in Chinese)

[13]王静毅, 陈业渊, 黄秉智, 于飞, 武耀廷. 部分香蕉品种SSR指纹图谱的构建. 果树学报, 2009, 26(5): 733-738.

Wang J Y, Chen Y Y, Huang B Z, Yu F, Wu Y T. Establishment of fingerprinting for bananas (Musa nana) by SSR marker. Journal of Fruit Science, 2009, 26(5): 733-738. (in Chinese)

[14]高运来, 朱荣胜, 刘春燕, 李文福, 蒋洪蔚, 李灿东, 姚丙晨, 胡国华, 陈庆山. 黑龙江部分大豆品种分子ID的构建. 作物学报, 2009, 35(2): 211-218.

Gao Y L, Zhu R S, Liu C Y, Li W F, Jiang H W, Li C D, Yao B C, Hu G H, Chen Q S. Establishment of molecular ID in soybean varieties in Heilongjiang, China. Acta Agronomica Sinica, 2009, 35(2): 211-218. (in Chinese)

[15]杨阳, 刘振, 赵洋, 梁国强. 湖南省主要茶树品种分子指纹图谱的构建. 茶叶科学, 2010, 30(5): 367-373.

Yang Y, Liu Z, Zhao Y, Liang G Q. Construction of DNA fingerprints for tea cultivars originated from Hunan province. Journal of Tea Science, 2010, 30(5): 367-373. (in Chinese)

[16]刘新龙, 马丽, 陈学宽, 应雄美, 蔡青, 刘家勇, 吴才文. 云南甘蔗自育品种DNA指纹身份证构建. 作物学报, 2010, 36(2): 202-210.

Liu X L, Ma L, Chen X K, Ying X M, Cai Q, Liu J Y, Wu C W. Establishment of DNA fingerprint ID in sugarcane cultivars in Yunnan, China. Acta Agronomica Sinica, 2010, 36(2): 202-210. (in Chinese)

[17]Creste S, Tulmann-Neto A, Figueira A. Detection of simple sequence repeats polymorphisms in denaturating polyacrylamide sequencing gels by silver staining. Plant Molecular Biology Reporter, 2001, 19(4): 299-306.

[18]Dirlewanger E, Cosson P, Tavaud M, Aranzana M J, Poizat C, Zanetto A, Arús P, Laigret F. Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.). Theoretical and Applied Genetics, 2002, 105(1): 127-138.

[19]Cipriani G, Lot G, Huang W G., Marrazzo M T, Peterlunger E, Testolin R. AC/GT and AG/CT microsatellite repeats in peach (Prunus persica (L.) Batsch): Isolation; characterisation and cross-species amplification in Prunus. Theoretical and Applied Genetics, 1999, 99(1/2): 65-72.

[20]Aranzana M J, Garcia-Mas J, Carbo J, Arús P. Development and variability analysis of microsatellite markers in peach. Plant Breeding, 2002, 121(1): 87-92.

[21]Romero C, Pedryc A, Muñoz V, Liácer G, Badenes M L. Genetic diversity of different apricot geographical groups determined by SSR markers. Genome, 2003, 46(2): 244-252.

[22]Testolin R, Marrazzo T, Cipriani G., Quarta R, Verde I, Dettori M T, Pancaldi M, Sansavini S. Microsatellite DNA in peach (Prunus persica (L.) Batsch) and its use infingerprinting and testing the genetic origin of cultivars. Genome, 2000, 43: 512-520.

[23]刘慧民, 刘一佳, 吕贵娥, 车代弟. 18种绣线菊分子身份证的构建和SCAR标记转化. 植物生理学通讯, 2009, 45(12): 1171-1176.

Liu H M, Liu Y J, Lü G E, Che D D. Establishment of molecular ID and conversion to SCAR marker in eighteen Spiraea species. Plant Physiology Communications, 2009, 45(12): 1171-1176. (in Chinese)

[24]郑海燕, 粟建光, 戴志刚, 李燕, 陈基权, 龚友才. 利用ISSR和RAPD标记构建红麻种质资源分子身份证.中国农业科学, 2010, 43(17): 3499-3510.

Zheng H Y, Su J G, Dai Z G, Li Y, Chen J Q, Gong Y C. Establishment of molecular identity for kenaf germplasm using ISSR and RAPD markers. Scientia Agricultura Sinica, 2010, 43(17): 3499-3510. (in Chinese)

[25]齐兰, 王文泉, 张振文, 叶剑秋, 李开绵. 利用SRAP标记构建18个木薯品种的DNA指纹图谱. 作物学报, 2010, 36(10): 1642-1648.

Qi L, Wang W Q, Zhang Z W, Ye J Q, Li K M. DNA fingerprinting analysis of 18 cassava varieties using sequence-related amplified polymorphism markers. Acta Agronomica Sinica, 2010, 36(10): 1642-1648. (in Chinese)