中国农业科学 ›› 2022, Vol. 55 ›› Issue (13): 2613-2628.doi: 10.3864/j.issn.0578-1752.2022.13.011
陈旭1(),郝雅琼1,聂兴华1,杨海莹1,刘松1,王雪峰2,曹庆芹1,秦岭1(),邢宇1()
收稿日期:
2021-10-18
接受日期:
2022-03-01
出版日期:
2022-07-01
发布日期:
2022-07-08
通讯作者:
秦岭,邢宇
作者简介:
陈旭,Tel:13463609439;E-mail: 基金资助:
CHEN Xu1(),HAO YaQiong1,NIE XingHua1,YANG HaiYing1,LIU Song1,WANG XueFeng2,CAO QingQin1,QIN Ling1(),XING Yu1()
Received:
2021-10-18
Accepted:
2022-03-01
Online:
2022-07-01
Published:
2022-07-08
Contact:
Ling QIN,Yu XING
摘要:
【目的】通过测定113份板栗品种(系)的数量、质量和假质量性状,分析其遗传变异,比较不同组群之间的性状差异,将SSR标记与性状进行关联,获得更多与SSR标记显著关联的性状,挖掘优异的等位变异位点,为开展板栗分子辅助育种的研究提供参考。【方法】测定并分析板栗总苞和坚果的38个数量、质量和假质量性状,利用SPSS和Graphpad软件对数量性状进行差异显著性和相关性分析,基于SSR标记进行遗传多样性分析,最后用TASSEL 2.1软件通过一般线性模型(general linear model,GLM)和混合线性模型(mixed linear model,MLM)分别对性状和标记进行关联分析。【结果】在遗传多样性分析中,21对SSR引物的有效等位基因数(Ne)、Shannon指数(I)、多态性信息含量(PIC>0.5)、观察杂合度(Ho)和期望杂合度(He)的平均值分别为3.164、1.269、0.589、0.593和0.635。根据群体结构分析分为2个主要组群,为了更好地比较性状差异,在聚类分析时将中间类型的品种单独划分为一组。在质量和假质量性状分析中,多样性指数变化范围为0.139—1.567,遗传多样性最高的性状是坚果光泽,最低的是底座接线;坚果形状、光泽、颜色等性状组群间频率分布差异明显。在数量性状分析中,变异系数的范围在3.96%—36.31%,苞重、总苞重和单粒重的变异系数均在30%以上,遗传变异程度高;果形指数和含水量变异系数均在10%以下,具有稳定的遗传特性;总苞和坚果的外观性状之间有强相关性,相关系数均在0.6以上;组1-组2和组2-组3在果实形状和重量中存在显著差异(P<0.05)。在关联分析中,GLM模型中有13个标记位点与18个表型性状极显著关联,表型变异解释率范围为15.12%—54.99%;MLM模型中有6个标记位点与7个表型性状极显著关联,表型变异解释率范围在8.66%—26.93%。【结论】本研究将SSR标记与表型性状进行关联分析,共发现13个标记位点与坚果单粒重等20个表型性状极显著关联,为开展板栗分子辅助育种的研究奠定了基础。
陈旭,郝雅琼,聂兴华,杨海莹,刘松,王雪峰,曹庆芹,秦岭,邢宇. 板栗总苞和坚果主要性状与SSR标记的关联分析[J]. 中国农业科学, 2022, 55(13): 2613-2628.
CHEN Xu,HAO YaQiong,NIE XingHua,YANG HaiYing,LIU Song,WANG XueFeng,CAO QingQin,QIN Ling,XING Yu. Association Analysis of Main Characteristics of Bur and Nut with SSR Markers in Chinese Chestnut[J]. Scientia Agricultura Sinica, 2022, 55(13): 2613-2628.
表1
SSR分子标记的多态性"
引物 Marker | 观察等位基因数 Na | 有效等位基因数 Ne | Shannon指数 I | 观测杂合度 Ho | 期望杂合度 He | 多态性信息含量 PIC |
---|---|---|---|---|---|---|
CmSI0396 | 4 | 2.553 | 1.069 | 0.708 | 0.608 | 0.542 |
CmSI0509 | 5 | 2.217 | 1.040 | 0.504 | 0.549 | 0.503 |
CmSI0561 | 8 | 1.988 | 1.132 | 0.460 | 0.497 | 0.477 |
CmSI0617 | 8 | 4.414 | 1.637 | 0.814 | 0.773 | 0.738 |
CmSI0658 | 6 | 4.454 | 1.589 | 0.796 | 0.775 | 0.740 |
CmSI0702 | 5 | 2.887 | 1.177 | 0.637 | 0.654 | 0.589 |
CmSI0735 | 6 | 2.753 | 1.199 | 0.646 | 0.637 | 0.568 |
CmSI0742 | 6 | 3.149 | 1.280 | 0.558 | 0.682 | 0.624 |
CmSI0800 | 13 | 5.240 | 1.959 | 0.708 | 0.809 | 0.786 |
CmSI0809 | 4 | 3.015 | 1.193 | 0.625 | 0.668 | 0.604 |
CmSI0853 | 6 | 3.835 | 1.518 | 0.681 | 0.739 | 0.699 |
CmSI0871 | 5 | 1.895 | 0.768 | 0.531 | 0.472 | 0.384 |
CmSI0883 | 6 | 3.776 | 1.531 | 0.681 | 0.735 | 0.702 |
CmSI0933 | 3 | 1.822 | 0.799 | 0.469 | 0.451 | 0.408 |
CmSI0938 | 4 | 1.774 | 0.740 | 0.204 | 0.436 | 0.368 |
CmSI0922 | 8 | 4.884 | 1.766 | 0.664 | 0.795 | 0.769 |
CmSI0930 | 11 | 4.617 | 1.838 | 0.779 | 0.783 | 0.761 |
CmSI0881 | 6 | 3.278 | 1.442 | 0.478 | 0.695 | 0.658 |
CmSI0614 | 6 | 4.464 | 1.590 | 0.805 | 0.776 | 0.740 |
CmSI0404 | 3 | 1.399 | 0.510 | 0.212 | 0.285 | 0.253 |
CmSI0747 | 3 | 2.042 | 0.880 | 0.496 | 0.510 | 0.456 |
均值 Average | 6 | 3.164 | 1.269 | 0.593 | 0.635 | 0.589 |
表2
板栗品种(系)质量性状的频率分布"
性状 Character | 频率分布 Frequency distribution (%) | 多样性指数H' Diversity index | ||||
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | ||
开裂方式Bur crack | 32.74 | 63.72 | 3.54 | 0.771 | ||
刺束分支角Prickle angle | 4.42 | 65.49 | 30.09 | 0.777 | ||
刺束密度Prickle density | 5.31 | 29.20 | 65.49 | 0.793 | ||
刺束颜色Prickle color | 11.50 | 88.50 | 0.357 | |||
坚果颜色均匀度Nut color evenness | 71.68 | 22.12 | 6.19 | 0.745 | ||
坚果光泽Nut glossiness | 21.24 | 30.09 | 13.27 | 20.35 | 15.04 | 1.567 |
果顶果肩Apex | 26.55 | 35.40 | 35.40 | 2.65 | 1.184 | |
茸毛分布Fuzz distribution | 56.64 | 22.12 | 21.24 | 0.985 | ||
茸毛颜色Fuzz color | 25.66 | 74.34 | 0.570 | |||
茸毛密度Fuzz density | 30.97 | 25.66 | 28.32 | 15.04 | 1.354 | |
筋线明显程度Obvious of stripes | 76.11 | 20.35 | 3.54 | 0.650 | ||
底座大小Hilum size | 9.73 | 50.44 | 39.82 | 0.939 | ||
底座光滑度Hilum smoothing | 44.25 | 55.75 | 0.687 | |||
涩皮剥离难易程度Difficulty of peeling inner skin | 12.39 | 87.61 | 0.375 | |||
刺苞形状Bur shape | 24.78 | 74.34 | 0.88 | 0.608 | ||
坚果颜色Nut color | 2.65 | 36.28 | 14.16 | 41.59 | 5.31 | 1.262 |
边果形状Nut shape | 77.88 | 22.12 | 0.528 | |||
底座接线Hilum margin | 97.35 | 0.88 | 1.77 | 0.139 | ||
种仁颜色Kernel color | 5.31 | 87.61 | 7.08 | 0.459 |
表3
板栗品种(系)的质量性状频率分布差异"
性状 Character | 组别 Groups | 频率分布 Frequency distribution (%) | ||||
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | ||
开裂方式 Bur crack | 1 | 47.06 | 47.06 | 5.88 | ||
2 | 29.33 | 68.00 | 2.67 | |||
3 | 33.33 | 61.90 | 4.76 | |||
刺束分支角 Prickle angle | 1 | 5.88 | 58.82 | 35.29 | ||
2 | 5.33 | 65.33 | 29.33 | |||
3 | 71.43 | 28.57 | ||||
刺束密度 Prickle density | 1 | 5.88 | 23.53 | 70.59 | ||
2 | 5.33 | 32.00 | 62.67 | |||
3 | 4.76 | 23.81 | 71.43 | |||
刺束颜色 Prickle color | 1 | 11.76 | 88.24 | |||
2 | 10.67 | 89.33 | ||||
3 | 14.29 | 85.71 | ||||
坚果颜色均匀度 Nut color evenness | 1 | 70.59 | 23.5 | 5.88 | ||
2 | 76.00 | 17.33 | 6.67 | |||
3 | 57.14 | 38.10 | 4.76 | |||
坚果光泽 Nut glossiness | 1 | 29.41 | 41.18 | 11.76 | 11.76 | 5.88 |
2 | 20.00 | 26.67 | 10.67 | 22.67 | 20.00 | |
3 | 19.05 | 33.33 | 23.81 | 19.05 | 4.76 | |
果顶果肩 Apex | 1 | 17.65 | 29.41 | 52.94 | ||
2 | 28.00 | 36.00 | 33.33 | 2.67 | ||
3 | 28.57 | 38.10 | 28.57 | 4.76 | ||
茸毛分布 Fuzz distribution | 1 | 88.24 | 5.88 | 5.88 | ||
2 | 48.00 | 25.33 | 26.67 | |||
3 | 61.90 | 23.81 | 14.29 | |||
茸毛颜色 Fuzz color | 1 | 23.53 | 76.47 | |||
2 | 26.67 | 73.33 | ||||
3 | 23.81 | 76.19 | ||||
茸毛密度 Fuzz density | 1 | 35.29 | 41.18 | 11.76 | 11.76 | |
2 | 29.33 | 18.67 | 32.00 | 20.00 | ||
3 | 33.33 | 38.10 | 28.57 | |||
筋线明显程度 Obvious of stripes | 1 | 76.47 | 23.53 | |||
2 | 78.67 | 16.00 | 5.33 | |||
3 | 66.67 | 33.33 | ||||
底座大小 Hilum size | 1 | 23.53 | 58.82 | 17.65 | ||
2 | 6.67 | 41.33 | 52.00 | |||
3 | 9.52 | 76.19 | 14.29 | |||
性状 Character | 组别 Groups | 频率分布 Frequency distribution (%) | ||||
1 | 2 | 3 | 4 | 5 | ||
底座光滑度 Hilum smoothing | 1 | 29.41 | 70.59 | |||
2 | 48.00 | 52.00 | ||||
3 | 42.86 | 57.14 | ||||
涩皮剥离难易程度 Difficulty of peeling inner skin | 1 | 23.53 | 76.47 | |||
2 | 9.33 | 90.67 | ||||
3 | 14.29 | 85.71 | ||||
刺苞形状 Bur shape | 1 | 17.65 | 82.35 | |||
2 | 24.00 | 74.67 | 1.33 | |||
3 | 33.33 | 66.67 | ||||
坚果颜色 Nut color | 1 | 17.65 | 70.59 | 11.76 | ||
2 | 2.67 | 42.67 | 18.67 | 33.33 | 2.67 | |
3 | 4.76 | 28.57 | 9.52 | 47.62 | 9.52 | |
边果形状 Nut shape | 1 | 47.06 | 52.94 | |||
2 | 85.33 | 14.67 | ||||
3 | 76.19 | 23.81 | ||||
底座接线 Hilum margin | 1 | 88.24 | 11.76 | |||
2 | 98.67 | 1.33 | ||||
3 | 100.00 | |||||
种仁颜色 Kernel color | 1 | 17.65 | 82.35 | |||
2 | 4.00 | 92.00 | 4.00 | |||
3 | 76.19 | 23.81 |
表4
板栗品种(系)主要数量性状的变异分析"
指标 Character | 极小值 Minimum | 极大值 Maximum | 平均值 Average | 标准差 S | 变异系数 CV (%) |
---|---|---|---|---|---|
总苞重 Total bur weight (g) | 11.93 | 120.83 | 65.91 | 22.02 | 33.41 |
苞重 Bur weight (g) | 7.76 | 79.95 | 38.66 | 14.04 | 36.31 |
刺苞宽Bur width (mm) | 50.80 | 105.31 | 80.54 | 10.77 | 13.38 |
刺苞厚Bur thickness (mm) | 42.58 | 88.06 | 66.45 | 8.14 | 12.25 |
刺苞高Bur height (mm) | 39.55 | 80.09 | 61.99 | 7.87 | 12.69 |
苞肉厚Bur skin thickness (mm) | 1.52 | 5.48 | 3.63 | 0.974 | 26.80 |
刺束长Prickle length (mm) | 6.80 | 18.66 | 13.77 | 2.21 | 16.08 |
刺苞的坚果数Nuts No./Bur | 1.38 | 3.10 | 2.50 | 0.305 | 12.21 |
单粒重Single nut weight (g) | 2.67 | 20.68 | 10.99 | 3.81 | 34.69 |
坚果厚Nut thickness (mm) | 13.86 | 32.05 | 20.60 | 3.25 | 15.76 |
坚果宽Nut width (mm) | 19.42 | 39.53 | 31.08 | 3.67 | 11.79 |
坚果高Nut height (mm) | 17.45 | 32.63 | 26.60 | 2.71 | 10.19 |
果形指数Nut shape index | 0.788 | 0.953 | 0.858 | 0.034 | 3.96 |
出实率Kernel yield (%) | 22.23 | 56.84 | 41.27 | 5.80 | 14.05 |
含水量Water content (%) | 44.46 | 60.69 | 52.03 | 3.57 | 6.85 |
可溶性糖含量Soluble sugar (%, DW) | 8.63 | 19.75 | 13.21 | 2.08 | 15.76 |
直链淀粉含量Amylose (%, DW) | 4.44 | 18.53 | 11.86 | 3.20 | 26.94 |
支链淀粉含量Amylopectin (%, DW) | 28.52 | 75.64 | 53.15 | 10.15 | 19.09 |
总淀粉含量Total starch (%, DW) | 40.22 | 85.18 | 65.01 | 9.39 | 14.45 |
表5
板栗品种(系)的数量性状差异比较"
指标 Character | 组1 Group1 | 组2 Group2 | 组3 Group3 |
---|---|---|---|
总苞重Total bur weight (g) | 56.29±17.47a | 72.26±21.37b | 51.03±16.40a |
苞重Bur weight (g) | 32.80±10.55a | 42.68±13.88b | 29.05±9.71a |
刺苞宽Bur width (mm) | 75.29±10.62a | 83.62±9.81b | 73.78±9.07a |
刺苞厚Bur thickness (mm) | 62.15±7.98a | 68.94±7.12b | 61.05±7.40a |
刺苞高Bur height (mm) | 57.34±7.09a | 64.58±6.58b | 56.54±7.96a |
苞肉厚Bur skin thickness (mm) | 3.30±0.82a | 3.84±0.96b | 3.18±0.89a |
刺束长Prickle length (mm) | 12.97±2.02a | 14.18±2.10a | 12.95±2.31a |
刺苞的坚果数Nuts No./Bur | 2.53±0.28a | 2.49±0.30a | 2.52±0.32a |
单粒重Single nut weight (g) | 9.24±3.05a | 12.03±3.74b | 8.67±2.87a |
坚果厚Nut thickness (mm) | 19.22±2.52a | 21.42±3.12b | 18.75±2.99a |
坚果宽Nut width (mm) | 29.78±3.47a | 32.06±3.34b | 28.63±3.35a |
坚果高Nut height (mm) | 25.63±2.71a | 27.32±2.38b | 24.82±2.71a |
果形指数Nut shape index | 0.86±0.04a | 0.85±0.04a | 0.87±0.02a |
出实率Kernel yield (%) | 41.69±4.50a | 40.71±6.31a | 42.95±4.10a |
含水量Water content (%) | 51.58±3.17a | 52.13±3.56a | 52.04±3.77a |
可溶性糖含量Soluble sugar (%, DW) | 12.62±2.11a | 13.19±1.98a | 13.83±2.19a |
直链淀粉含量Amylose (%, DW) | 12.94±2.93a | 11.77±3.26a | 11.21±2.81a |
支链淀粉含量Amylopectin (%, DW) | 52.51±11.56a | 52.82±9.68a | 55.09±9.96a |
总淀粉含量Total starch (%, DW) | 65.45±10.82a | 64.58±9.02a | 66.29±8.96a |
表6
GLM和MLM模型的关联分析结果"
性状 Character | 位点 Loci | GLM | MLM | ||
---|---|---|---|---|---|
P<0.01 | R2 (%) | P<0.01 | R2 (%) | ||
总苞重Total bur weight | CmSI0883 | 0.0017 | 36.84 | ||
CmSI0930 | 0.0021 | 46.90 | |||
苞重Bur weight | CmSI0883 | 0.0023 | 36.43 | ||
CmSI0930 | 0.001 | 49.07 | 0.0095 | 16.31 | |
刺苞宽Bur width | CmSI0883 | 0.0072 | 33.37 | ||
刺苞厚Bur thickness | CmSI0883 | 0.0045 | 33.90 | ||
刺苞高Bur height | CmSI0883 | 7.96E-04 | 37.47 | ||
刺束长Prickle length | CmSI0702 | 0.0018 | 28.87 | ||
CmSI0883 | 0.0084 | 34.38 | |||
刺苞的坚果数Nuts No./Bur | CmSI0800 | 0.0074 | 53.33 | 0.0027 | 19.99 |
单粒重Single nut weight | CmSI0853 | 0.0021 | 38.84 | ||
CmSI0930 | 0.0092 | 42.86 | |||
坚果宽Nut width | CmSI0930 | 0.0065 | 45.21 | ||
坚果高Nut height | CmSI0883 | 0.0035 | 36.59 | ||
含水量Water content | CmSI0735 | 6.71E-04 | 36.42 | ||
可溶性糖含量Soluble sugar | CmSI0800 | 0.0085 | 26.93 | ||
刺束密度Prickle density | CmSI0735 | 0.0064 | 26.79 | 0.0065 | 8.66 |
CmSI0742 | 0.0053 | 29.04 | |||
坚果颜色均匀度Nut color evenness | CmSI0871 | 8.14E-05 | 30.05 | ||
筋线明显程度Obvious of stripes | CmSI0800 | 0.0037 | 20.21 | ||
涩皮剥离难易程度Difficulty of peeling inner skin | CmSI0561 | 7.94E-05 | 42.52 | ||
刺苞形状Bur shape | CmSI0404 | 0.0061 | 15.12 | ||
坚果颜色Nut color | CmSI0881 | 0.0076 | 36.49 | ||
底座接线Hilum margin | CmSI0509 | 1.73E-06 | 45.52 | 1.20E-03 | 14.41 |
CmSI0881 | 5.96E-07 | 54.99 | 3.60E-03 | 16.29 | |
种仁颜色Kernel color | CmSI0617 | 0.0037 | 47.72 | 0.0041 | 17.93 |
CmSI0881 | 0.0033 | 37.95 | 0.0013 | 15.01 |
[1] |
武燕奇, 郭素娟. 5个板栗品种(系)对持续干旱胁迫和复水的生理响应. 中南林业科技大学学报, 2017, 37(10): 67-74. doi: 10.14067/j.cnki.1673-923x.2017.10.011.
doi: 10.14067/j.cnki.1673-923x.2017.10.011 |
WU Y Q, GUO S J. Physiological Response of five Chinese chestnut varieties (clone) after drought stress and rewatering. Journal of Central South University of Forestry & Technology, 2017, 37(10): 67-74. doi: 10.14067/j.cnki.1673-923x.2017.10.011. (in Chinese)
doi: 10.14067/j.cnki.1673-923x.2017.10.011 |
|
[2] | FAO. 2019. Value of agricultural production. http://faostat3.fao.org/. |
[3] | 田寿乐, 明桂冬, 张美勇, 单公华, 徐颖, 许林. 中国板栗育种进展. 第五届全国干果生产、科研进展学术研讨会论文集. 北京: 中国农业科学技术出版社, 2007: 92-97. |
TIAN S L, MING G D, ZHANG M Y, SHAN G H, XU Y, XU L. Advances in Chinese Chestnut breeding// Proceedings of the 5th National Conference on Dry Fruit Production and Scientific Research Progress. Beijing: China Agricultural Science and Technology Press, 2007: 92-97. (in Chinese) | |
[4] |
FONT I FORCADA C, ORAGUZIE N, IGARTUA E, MORENO M Á, GOGORCENA Y. Population structure and marker-trait associations for pomological traits in peach and nectarine cultivars. Tree Genetics & Genomes, 2013, 9(2): 331-349. doi: 10.1007/s11295-012-0553-0.
doi: 10.1007/s11295-012-0553-0 |
[5] |
左力辉, 张文林, 邱彤, 张军, 杨敏生. 新疆野苹果叶形性状变异及其与SSR标记关联分析. 园艺学报, 2015, 42(4): 759-768. doi: 10.16420/j.issn.0513-353x.2014-1030.
doi: 10.16420/j.issn.0513-353x.2014-1030 |
ZUO L H, ZHANG W L, QIU T, ZHANG J, YANG M S. Malus sieversii leaf traits variation and correlation analysis with SSR markers. Acta Horticulturae Sinica, 2015, 42(4): 759-768. doi: 10.16420/j.issn.0513-353x.2014-1030. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2014-1030 |
|
[6] |
FRESNEDO-RAMIREZ J, BINK M C A M, VAN DE WEG W E, FAMULA T R, CRISOSTO C H, TRETT T, GASIC K, GRADZIEL T M. QTL mapping of pomological traits in peach and related species breeding germplasm. Molecular Breeding, 2015, 35: 166.
doi: 10.1007/s11032-015-0357-7 |
[7] | 聂兴华, 郑瑞杰, 赵永廉, 曹庆芹, 秦岭, 邢宇. 利用荧光SSR分子标记评估中国栗属植物遗传多样性. 中国农业科学, 2021, 54(8): 1739-1753. |
NIE X H, ZHENG R J, ZHAO Y L, CAO Q Q, QIN L, XING Y. Genetic diversity evaluation of Castanea in China based on fluorescently labeled SSR. Scientia Agricultura Sinica, 2021, 54(8): 1739-1753. (in Chinese) | |
[8] |
张馨方, 张树航, 李颖, 郭燕, 王广鹏. 基于SSR标记的燕山板栗种质资源遗传多样性分析. 中国农业大学学报, 2020, 25(4): 61-71. doi: 10.11841/j.issn.1007-4333.2020.04.07.
doi: 10.11841/j.issn.1007-4333.2020.04.07 |
ZHANG X F, ZHANG S H, LI Y, GUO Y, WANG G P. Genetic diversity analysis of chestnut germplasm in Yanshan region based on SSR markers. Journal of China Agricultural University, 2020, 25(4): 61-71. doi: 10.11841/j.issn.1007-4333.2020.04.07. (in Chinese)
doi: 10.11841/j.issn.1007-4333.2020.04.07 |
|
[9] |
江锡兵, 龚榜初, 刘庆忠, 陈新, 吴开云, 邓全恩, 汤丹. 中国板栗地方品种重要农艺性状的表型多样性. 园艺学报, 2014, 41(4): 641-652. doi: 10.16420/j.issn.0513-353x.2014.04.008.
doi: 10.16420/j.issn.0513-353x.2014.04.008 |
JIANG X B, GONG B C, LIU Q Z, CHEN X, WU K Y, DENG Q E, TANG D. Phenotypic diversity of important agronomic traits of local cultivars of Chinese chestnut. Acta Horticulturae Sinica, 2014, 41(4): 641-652. doi: 10.16420/j.issn.0513-353x.2014.04.008. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2014.04.008 |
|
[10] |
李颖, 张树航, 郭燕, 张馨方, 王广鹏. 211份板栗种质资源花序表型多样性和聚类分析. 中国农业科学, 2020, 53(22): 4667-4682. doi: 10.3864/j.issn.0578-1752.2020.22.013.
doi: 10.3864/j.issn.0578-1752.2020.22.013 |
LI Y, ZHANG S H, GUO Y, ZHANG X F, WANG G P. Catkin phenotypic diversity and cluster analysis of 211 Chinese chestnut germplasms. Scientia Agricultura Sinica, 2020, 53(22): 4667-4682. doi: 10.3864/j.issn.0578-1752.2020.22.013. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2020.22.013 |
|
[11] |
ZHEBENTYAYEVA T N, SISCO P H, GEORGI L L, JEFFERS S N, PERKINS M T, JAMES J B, HEBARD F V, SASKI C, NELSON C D, ABBOTT A G. Dissecting resistance to Phytophthora cinnamomi in interspecific hybrid chestnut crosses using sequence-based genotyping and QTL mapping. Phytopathology, 2019, 109(9): 1594-1604.
doi: 10.1094/PHYTO-11-18-0425-R |
[12] |
KUBISIAK T L, NELSON C D, STATON M E, ZHEBENTYAYEVA T, SMITH C, OLUKOLU B A, FANG G C, HEBARD F V, ANAGNOSTAKIS S, WHEELER N, SISCO P H, ABBOTT A G, SEDEROFF R R. A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica). Tree Genetics & Genomes, 2013, 9(2): 557-571. doi: 10.1007/s11295-012-0579-3.
doi: 10.1007/s11295-012-0579-3 |
[13] |
JI F Y, WEI W, LIU Y, WANG G P, ZHANG Q, XING Y, ZHANG S H, LIU Z H, CAO Q Q, QIN L. Construction of a SNP-based high-density genetic map using genotyping by sequencing (GBS) and QTL analysis of nut traits in Chinese chestnut (Castanea mollissima Blume). Frontiers in Plant Science, 2018, 9: 816. doi: 10.3389/fpls.2018.00816.
doi: 10.3389/fpls.2018.00816 |
[14] |
NISHIO S, HAYASHI T, YAMAMOTO T, TERAKAMI S, IWATA H, IMAI A, TAKADA N, KATO H, SAITO T. Bayesian genome-wide association study of nut traits in Japanese chestnut. Molecular Breeding, 2018, 38(8): 1-16. doi: 10.1007/s11032-018-0857-3.
doi: 10.1007/s11032-018-0857-3 |
[15] |
JIANG X B, TANG D, GONG B C. Genetic diversity and association analysis of Chinese chestnut (Castanea mollissima Blume) cultivars based on SSR markers. Brazilian Journal of Botany, 2017, 40(1): 235-246. doi: 10.1007/s40415-016-0321-8.
doi: 10.1007/s40415-016-0321-8 |
[16] | 周必成, 欧阳绍湘, 周建仁, 黄发吉, 史涛, 屠和平, 熊冬连, 徐育海, 刘小武, 杨玉林, 许红霞. LY/T 1851-2009, 植物新品种特异性、一致性、稳定性测试指南——板栗[S]. 北京: 中国标准出版社, 2009. |
ZHOU B C, OUYANG S X, ZHOU J R, HUANG F J, SHI T, TU H P, XIONG D L, XU Y H, LIU X W, YANG Y L, XU H X. LY/T 1851-2009, Guidelines for the conduct of tests for distinctness, uniformity and stability-Chestnut (Castanea mollissima Bl.). Beijing: Standards Press of China, 2009. (in Chinese) | |
[17] | 刘庆忠. 板栗种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006. |
LIU Q Z. Descriptors and Data Standard for Chestnut (Castanea mollissima Blume). Beijing: Chinese Agriculture Press, 2006. (in Chinese) | |
[18] | UPOV. Guidelines for the conduct of tests for distinctness, uniformity and stability: Chestnut (Castanea mollissima Bl.) 2017.TG/124/4. |
[19] | 国家质量监督检验检疫总局, 中国国家标准化管理委员会. 植物新品种特异性、一致性和稳定性测试指南总则:GB/T 19557.1—2004[S]. 北京: 中国标准出版社, 2004. |
General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, Standardization Administration of the People’s Republic of China. General directives for the conduct of tests of distinctness, uniformity and stability for new varieties of plants:GB/T 19557.1—2004[S]. Beijing: Standards Press of China, 2004. (in Chinese) | |
[20] |
纪飞杨, 张惠真, 王广鹏, 张树航, 张卿, 房克凤, 邢宇, 曹庆芹, 秦岭. 板栗正反交后代坚果性状遗传倾向研究. 北京农学院学报, 2018, 33(4): 14-18. doi: 10.13473/j.cnki.issn.1002-3186.2018.0403.
doi: 10.13473/j.cnki.issn.1002-3186.2018.0403 |
JI F Y, ZHANG H Z, WANG G P, ZHANG S H, ZHANG Q, FANG K F, XING Y, CAO Q Q, QIN L. Studies on genetic tendency of nut characters in the progenies of reciprocal crosses in Castanea mollissima. Journal of Beijing University of Agriculture, 2018, 33(4): 14-18. doi: 10.13473/j.cnki.issn.1002-3186.2018.0403. (in Chinese)
doi: 10.13473/j.cnki.issn.1002-3186.2018.0403 |
|
[21] |
曹小艳, 李志, 张卿, 秦岭, 邢宇. 不同板栗品种(系)抗性淀粉综合评价. 中国粮油学报, 2019, 34(7): 39-46. doi: 10.3969/j.issn.1003-0174.2019.07.008.
doi: 10.3969/j.issn.1003-0174.2019.07.008 |
CAO X Y, LI Z, ZHANG Q, QIN L, XING Y. Comprehensive evaluation of resistant starch in different Chinese chestnut varieties (lines). Journal of the Chinese Cereals and Oils Association, 2019, 34(7): 39-46. doi: 10.3969/j.issn.1003-0174.2019.07.008. (in Chinese)
doi: 10.3969/j.issn.1003-0174.2019.07.008 |
|
[22] |
曹庆芹, 徐月, 冯永庆, 张瑞娟, 韩子德, 秦岭. 板栗基因组DNA不同提取方法的比较. 中国农学通报, 2007, 23(6): 160-163. doi: 10.3969/j.issn.1000-6850.2007.06.036.
doi: 10.3969/j.issn.1000-6850.2007.06.036 |
CAO Q Q, XU Y, FENG Y Q, ZHANG R J, HAN Z D, QIN L. Comparison studies on extracting genomic DNA of chestnut(Castanea mollissima. BL) by different methods. Chinese Agricultural Science Bulletin, 2007, 23(6): 160-163. doi: 10.3969/j.issn.1000-6850.2007.06.036. (in Chinese)
doi: 10.3969/j.issn.1000-6850.2007.06.036 |
|
[23] |
NIE X H, WANG Z H, LIU N W, SONG L, YAN B Q, XING Y, ZHANG Q, FANG K F, ZHAO Y L, CHEN X, WANG G P, QIN L, CAO Q Q. Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers. Journal of Integrative Agriculture, 2021, 20(5): 1277-1286.
doi: 10.1016/S2095-3119(20)63400-1 |
[24] | BRYMAN A, CRAMER D. Quantitative data analysis with IBM SPSS 17, 18 & 19: A guide for social scientists. Routledge, 2012. |
[25] |
MITTEER D R, GREER B D, FISHER W W, COHRS V L. Teaching behavior technicians to create publication-quality, single-case design graphs in graphpad prism 7. Journal of Applied Behavior Analysis, 2018, 51(4): 998-1010. doi: 10.1002/jaba.483.
doi: 10.1002/jaba.483 |
[26] |
FALUSH D, STEPHENS M, PRITCHARD J K. Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics, 2003, 164(4): 1567-1587. doi: 10.1093/genetics/164.4.1567.
doi: 10.1093/genetics/164.4.1567 |
[27] |
LIU K J, MUSE S V. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21(9): 2128-2129. doi: 10.1093/bioinformatics/bti282.
doi: 10.1093/bioinformatics/bti282 |
[28] |
PEAKALL R, SMOUSE P E. GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-An update. Bioinformatics, 2012, 28(19): 2537-2539. doi: 10.1093/bioinformatics/bts460.
doi: 10.1093/bioinformatics/bts460 |
[29] |
PEAKALL R, SMOUSE P E. GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 2006, 6(1): 288-295.
doi: 10.1111/j.1471-8286.2005.01155.x |
[30] |
张彦军, 苟作旺, 王兴荣, 李玥, 祁旭升. 西北地区和尚头小麦遗传多样性及农艺性状的关联分析. 草业学报, 2019, 28(2): 142-155. doi: 10.11686/cyxb2018124.
doi: 10.11686/cyxb2018124 |
ZHANG Y J, GOU Z W, WANG X R, LI Y, QI X S. An analysis of genetic diversity and linked agronomic traits of Heshangtou wheat in northwest China. Acta Prataculturae Sinica, 2019, 28(2): 142-155. doi: 10.11686/cyxb2018124. (in Chinese)
doi: 10.11686/cyxb2018124 |
|
[31] |
刘亚斌, 郭素娟, 孙传昊. 基于巢式分组设计的板栗刺苞与坚果形态多样性分析. 中南林业科技大学学报, 2020, 40(10): 51-60. doi: 10.14067/j.cnki.1673-923x.2020.10.006.
doi: 10.14067/j.cnki.1673-923x.2020.10.006 |
LIU Y B, GUO S J, SUN C H. Morphological diversity analysis of chestnut thorns bract and nuts based on nested grouping design. Journal of Central South University of Forestry & Technology, 2020, 40(10): 51-60. doi: 10.14067/j.cnki.1673-923x.2020.10.006. (in Chinese)
doi: 10.14067/j.cnki.1673-923x.2020.10.006 |
|
[32] |
苏淑钗, 林莉, 邓钰薪, 雷恒久. 华北品种群板栗品质的综合评价. 经济林研究, 2009, 27(2): 20-27. doi: 10.3969/j.issn.1003-8981.2009.02.006.
doi: 10.3969/j.issn.1003-8981.2009.02.006 |
SU S C, LIN L, DENG Y X, LEI H J. Synthetic evaluation of chestnut cultivar groups in North China. Nonwood Forest Research, 2009, 27(2): 20-27. doi: 10.3969/j.issn.1003-8981.2009.02.006. (in Chinese)
doi: 10.3969/j.issn.1003-8981.2009.02.006 |
|
[33] |
赖俊声, 江锡兵, 龚榜初, 杨龙, 汤丹. 板栗地方品种质量性状多样性分析. 浙江农业科学, 2016, 57(8): 1196-1200. doi: 10.16178/j.issn.0528-9017.20160814.
doi: 10.16178/j.issn.0528-9017.20160814 |
LAI J S, JIANG X B, GONG B C, YANG L, TANG D. Diversity analysis of quality traits of local varieties of Castanea mollissima. Journal of Zhejiang Agricultural Sciences, 2016, 57(8): 1196-1200. doi: 10.16178/j.issn.0528-9017.20160814. (in Chinese)
doi: 10.16178/j.issn.0528-9017.20160814 |
|
[34] |
刘国彬, 兰彦平, 兰卫宗, 曹均. 板栗农家品种资源坚果表型性状分析. 江西农业大学学报, 2013, 35(5): 977-981, 987. doi: 10.13836/j.jjau.2013171.
doi: 10.13836/j.jjau.2013171 |
LIU G B, LAN Y P, LAN W Z, CAO J. An analysis of phenotypic traits of native chestnut varieties. Acta Agriculturae Universitatis Jiangxiensis, 2013, 35(5): 977-981, 987. doi: 10.13836/j.jjau.2013171. (in Chinese)
doi: 10.13836/j.jjau.2013171 |
|
[35] |
刘国彬, 曹均, 兰彦平, 王金宝, 刘建玲, 兰卫宗. 板栗总苞与坚果表型多样性及其相关关系研究. 经济林研究, 2014, 32(2): 28-33. doi: 10.14067/j.cnki.1003-8981.2014.02.009.
doi: 10.14067/j.cnki.1003-8981.2014.02.009 |
LIU G B, CAO J, LAN Y P, WANG J B, LIU J L, LAN W Z. Phenotypic diversity of involucres and nut in Castanea mollissima and their relationship. Nonwood Forest Research, 2014, 32(2): 28-33. doi: 10.14067/j.cnki.1003-8981.2014.02.009. (in Chinese)
doi: 10.14067/j.cnki.1003-8981.2014.02.009 |
|
[36] | 江锡兵, 龚榜初, 汤丹, 刘庆忠, 陈新, 吴开云, 邓全恩. 中国部分板栗品种坚果表型及营养成分遗传变异分析. 西北植物学报, 2013, 33(11): 2216-2224. |
JIANG X B, GONG B C, TANG D, LIU Q Z, CHEN X, WU K Y, DENG Q E. Genetic variation of nut phenotype and nutrient of some of Chinese chestnut cultivars. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(11): 2216-2224. (in Chinese) | |
[37] |
马玉敏, 陈学森, 何天明, 吴传金, 王娜. 中国板栗3个野生居群部分表型性状的遗传多样性. 园艺学报, 2008, 35(12): 1717-1726. doi: 10.16420/j.issn.0513-353x.2008.12.001.
doi: 10.16420/j.issn.0513-353x.2008.12.001 |
MA Y M, CHEN X S, HE T M, WU C J, WANG N. Genetic diversity of morphological traits in wild populations of Castanea mollissima blume. Acta Horticulturae Sinica, 2008, 35(12): 1717-1726. doi: 10.16420/j.issn.0513-353x.2008.12.001. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2008.12.001 |
|
[38] |
刘希强, 张涵, 王学敏, 仪登霞, 王赞. 紫花苜蓿秋眠性的SSR标记关联分析. 中国农业科学, 2018, 51(2): 226-232. doi: 10.3864/j.issn.0578-1752.2018.02.003.
doi: 10.3864/j.issn.0578-1752.2018.02.003 |
LIU X Q, ZHANG H, WANG X M, YI D X, WANG Z. Association mapping of fall dormancy with SSR markers in alfalfa (Medicago sativa L.). Scientia Agricultura Sinica, 2018, 51(2): 226-232. doi: 10.3864/j.issn.0578-1752.2018.02.003. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2018.02.003 |
|
[39] |
侯丽媛, 董艳辉, 张春芬, 肖蓉, 邓舒, 聂园军, 赵菁, 曹秋芬. SSR分子标记技术在苹果种质资源及遗传育种研究中的应用. 山西农业科学, 2019, 47(6): 1107-1114. doi: 10.3969/j.issn.1002-2481.2019.06.41.
doi: 10.3969/j.issn.1002-2481.2019.06.41 |
HOU L Y, DONG Y H, ZHANG C F, XIAO R, DENG S, NIE Y J, ZHAO J, CAO Q F. Application of SSR molecular markers in germplasm resources and genetic breeding of apple. Journal of Shanxi Agricultural Sciences, 2019, 47(6): 1107-1114. doi: 10.3969/j.issn.1002-2481.2019.06.41. (in Chinese)
doi: 10.3969/j.issn.1002-2481.2019.06.41 |
|
[40] |
江锡兵, 汤丹, 龚榜初, 赖俊声. 基于SSR标记的板栗地方品种遗传多样性与关联分析. 园艺学报, 2015, 42(12): 2478-2488. doi: 10.16420/j.issn.0513-353x.2015-0484.
doi: 10.16420/j.issn.0513-353x.2015-0484 |
JIANG X B, TANG D, GONG B C, LAI J S. Genetic diversity and association analysis of local cultivars of Chinese chestnut based on SSR markers. Acta Horticulturae Sinica, 2015, 42(12): 2478-2488. doi: 10.16420/j.issn.0513-353x.2015-0484. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2015-0484 |
|
[41] |
袁欣捷, 方荣, 周坤华, 雷刚, 黄月琴, 陈学军. 辣椒重要农艺性状关联分析与优异等位变异发掘. 核农学报, 2020, 34(12): 2658-2672. doi: 10.11869/j.issn.100-8551.2020.12.2658.
doi: 10.11869/j.issn.100-8551.2020.12.2658 |
YUAN X J, FANG R, ZHOU K H, LEI G, HUANG Y Q, CHEN X J. Association analysis of important agronomic traits in pepper (Capsicum annuum L.) and mining of elite alleles. Journal of Nuclear Agricultural Sciences, 2020, 34(12): 2658-2672. doi: 10.11869/j.issn.100-8551.2020.12.2658. (in Chinese)
doi: 10.11869/j.issn.100-8551.2020.12.2658 |
|
[42] |
ZHANG Z W, ERSOZ E, LAI C Q, TODHUNTER R J, TIWARI H K, GORE M A, BRADBURY P J, YU J M, ARNETT D K, ORDOVAS J M, BUCKLER E S. Mixed linear model approach adapted for genome-wide association studies. Nature Genetics, 2010, 42(4): 355-360. doi: 10.1038/ng.546.
doi: 10.1038/ng.546 |
[43] |
PRICE A L, ZAITLEN N A, REICH D, PATTERSON N. New approaches to population stratification in genome-wide association studies. Nature Reviews Genetics, 2010, 11(7): 459-463. doi: 10.1038/nrg2813.
doi: 10.1038/nrg2813 |
[1] | 刘针杉, 涂红霞, 周荆婷, 马艳, 柴久凤, 王旨意, 杨鹏飞, 杨小芹, Kumail Abbas, 王浩, 王燕, 王小蓉. 中国樱桃正反交F1代果实主要性状的遗传分析[J]. 中国农业科学, 2023, 56(2): 345-356. |
[2] | 李周帅,董远,李婷,冯志前,段迎新,杨明羡,徐淑兔,张兴华,薛吉全. 基于杂交种群体的玉米产量及其配合力的全基因组关联分析[J]. 中国农业科学, 2022, 55(9): 1695-1709. |
[3] | 职蕾,者理,孙楠楠,杨阳,Dauren Serikbay,贾汉忠,胡银岗,陈亮. 小麦苗期铅耐受性的全基因组关联分析[J]. 中国农业科学, 2022, 55(6): 1064-1081. |
[4] | 王凯,张海亮,董祎鑫,陈少侃,郭刚,刘林,王雅春. 基于牧场管理数据的奶牛健康性状定义及遗传参数估计[J]. 中国农业科学, 2022, 55(6): 1227-1240. |
[5] | 郭燕, 张树航, 李颖, 张馨方, 王广鹏. 中国板栗36个叶片表型性状的多样性[J]. 中国农业科学, 2022, 55(5): 991-1009. |
[6] | 姜芬芬, 孙磊, 刘方东, 王吴彬, 邢光南, 张焦平, 张逢凯, 李宁, 李艳, 贺建波, 盖钧镒. 世界大豆生育阶段光温综合反应的地理分化和演化[J]. 中国农业科学, 2022, 55(3): 451-466. |
[7] | 李恒,字向东,王会,熊燕,吕明杰,刘宇,蒋旭东. 基于全基因组重测序的山羊产羔数性状关键调控基因的筛选[J]. 中国农业科学, 2022, 55(23): 4753-4768. |
[8] | 朱延松,张亚飞,程莉,杨胜男,赵婉彤,江东. 利用Target SSR-seq技术鉴定60份柑橘种质资源[J]. 中国农业科学, 2022, 55(22): 4458-4472. |
[9] | 逄洪波, 程露, 于茗兰, 陈强, 李玥莹, 吴隆坤, 王泽, 潘孝武, 郑晓明. 栽培稻芽期耐低温全基因组关联分析[J]. 中国农业科学, 2022, 55(21): 4091-4103. |
[10] | 刘树森,孙华,石洁,郭宁,马红霞,张海剑. 基于地上部症状的玉米根腐病分级及严重度与农艺性状的关系[J]. 中国农业科学, 2022, 55(20): 3939-3947. |
[11] | 姜朋, 张鹏, 姚金保, 吴磊, 何漪, 李畅, 马鸿翔, 张旭. 宁麦系列小麦品种的性状特点及相关基因位点分析[J]. 中国农业科学, 2022, 55(2): 233-247. |
[12] | 谢晓宇, 王凯鸿, 秦晓晓, 王彩香, 史春辉, 宁新柱, 杨永林, 秦江鸿, 李朝周, 马麒, 宿俊吉. 陆地棉吐絮率的限制性两阶段多位点全基因组关联分析及候选基因预测[J]. 中国农业科学, 2022, 55(2): 248-264. |
[13] | 刘丰,蒋佳丽,周琴,蔡剑,王笑,黄梅,仲迎鑫,戴廷波,曹卫星,姜东. 美国软麦籽粒品质变化趋势及对我国弱筋小麦标准达标度分析[J]. 中国农业科学, 2022, 55(19): 3723-3737. |
[14] | 李晓川,王朝海,周平,马维,吴瑞,宋治豪,梅艳. 马铃薯品种(系)田间晚疫病抗性评价和全基因组遗传多样性分析[J]. 中国农业科学, 2022, 55(18): 3484-3500. |
[15] | 张维理,张认连,张定祥,白占国,张晶,师华定. 世界主要国家土壤调查工作回顾[J]. 中国农业科学, 2022, 55(18): 3565-3583. |
|