广泛靶向代谢组学解析桃蚜危害对桃树次生代谢产物的影响

doi:10.3864/j.issn.0578-1752.2022.06.008

摘要/Abstract

摘要：

【目的】阐明桃蚜（Myzus persicae）危害条件下桃树抗蚜和感蚜品种的代谢响应机制,并确定桃树响应桃蚜危害过程中起关键作用的次生代谢产物。【方法】采用抗蚜和感蚜桃树幼嫩新梢进行桃蚜危害处理,取接种3 d的桃树新梢进行次生代谢产物提取,并利用超高效液相色谱串联质谱（UPLC-MS/MS）对抗蚜品种（品系）‘96-5-1’（9651）和枣油桃（ZYT）,感蚜品种中油13号（CN13）和中农金辉（ZNJH）进行广泛靶向代谢组分析,以|log₂ fold change|≥1,P-value≤0.01作为筛选差异代谢物（differentially altered metabolite,DAM）的阈值。采用OPLS-DA模型的VIP值表示抗蚜和感蚜品种之间的差异。【结果】对桃蚜危害3 d后的处理取样,处理后的样品进行广泛靶向代谢组学分析,全部样品中共鉴定出528种代谢物。通过主成分分析（PCA）、层次聚类分析（HCA）和维恩图分析,发现感蚜品种中油13号被桃蚜危害后的差异代谢物总数为7种,有2种含量下降,5种含量上升;中农金辉被桃蚜危害后的差异代谢物总数为7种,有3种含量下降,4种含量上升。抗蚜品种‘96-5-1’被桃蚜危害后的差异代谢物总数为33种,有1种含量下降,32种含量上升;枣油桃被桃蚜危害后的差异代谢物总数为55种,有12种含量下降,43种含量上升。大多数差异代谢物来自两个抗蚜品种,并且抗蚜品种中差异代谢物的变化幅度比感蚜品种更大。经过分析获得的15种次生代谢产物（6种氨基酸及其衍生物、5种酚酸、3种核苷酸及其衍生物、1种有机酸）可能与寿星桃来源的桃蚜抗性相关。【结论】抗蚜品种在桃蚜危害后显著上调的次生代谢产物可能参与桃树对桃蚜的抗性反应过程,这些次生代谢产物（氨基酸及其衍生物、酚酸类物质、核苷酸及其衍生物和有机酸）的调控是桃树抵抗桃蚜危害的重要机制。

关键词: 桃树, 桃蚜, 抗性, 代谢组学, 次生代谢产物, 超高效液相色谱串联质谱

Abstract:

【Objective】The objective of this study is to clarify the underlying biochemical mechanisms related to the resistance and susceptibility of peach towards Myzus persicae, and to identify the key secondary metabolites of peach responding to the M. persicae infection.【Method】New shoots of resistant (‘96-5-1’ (9651), ZaoYouTao (ZYT)) and susceptible (Zhong You 13 (CN13), Zhong Nong Jin Hui (ZNJH)) peach trees were inoculated with M. persicae for 3 days and used for secondary metabolite extraction and UPLC-MS/MS analysis. Differentially altered metabolites (DAMs) were screened with |log₂ fold change|≥1, P-value≤0.01 as threshold. The VIP value of the OPLS-DA model was used to perform differences between resistant and susceptible peach.【Result】To illustrate the biochemical mechanisms of M. persicae resistance in peach, aphid-resistant/aphid-susceptible peach varieties infested with M. persicae for 3 days. A total of 528 metabolites were identified in the treated samples through widely targeted metabolomics analysis. Using principal component analysis (PCA), hierarchical cluster analysis (HCA) and Venn diagram analysis, it was found that 7 DAMs were identified from the susceptible variety CN13, and 2 of them were significantly decreased, 5 of them were significantly increased. 7 DAMs were identified from the susceptible variety ZNJH, and 3 of them were significantly decreased, 4 of them were significantly increased. 33 DAMs were identified from the resistant variety ‘96-5-1’, and 1 of them was significantly decreased, 32 of them were significantly increased. 55 DAMs were identified from the resistant variety ZYT, and 12 of them were significantly decreased, 43 of them were significantly increased. The majority of the DAMs were identified from the two resistant varieties, and the overall magnitude of change was greater in the resistant varieties than that in the susceptible varieties. Finally, 15 secondary metabolites (6 amino acids and their derivatives, 5 phenolic acids, 3 nucleotides and their derivatives, and 1 organic acid) were considered to be involved in M. persicae resistance of ‘Shou xing tao’.【Conclusion】The significantly up-regulated secondary metabolites obtained in M. persicae-resistant peach varieties were mainly involved in the response to M. persicae feeding. The regulation of these secondary metabolites (amino acids and their derivatives, phenolic acids, nucleotides and their derivatives, organic acid) is the important mechanism of defense reaction to M. persicae.

Key words: peach, green peach aphid (Myzus persicae), resistance, metabolomics, secondary metabolite, UPLC-MS/MS

闫乐乐,卜璐璐,牛良,曾文芳,鲁振华,崔国朝,苗玉乐,潘磊,王志强. 广泛靶向代谢组学解析桃蚜危害对桃树次生代谢产物的影响[J]. 中国农业科学, 2022, 55(6): 1149-1158.

YAN LeLe,BU LuLu,NIU Liang,ZENG WenFang,LU ZhenHua,CUI GuoChao,MIAO YuLe,PAN Lei,WANG ZhiQiang. Widely Targeted Metabolomics Analysis of the Effects of Myzus persicae Feeding on Prunus persica Secondary Metabolites[J]. Scientia Agricultura Sinica, 2022, 55(6): 1149-1158.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2022.06.008

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序号 Code	代谢物种类 Species of metabolites	数目 Number
1	黄酮类Flavonoid	129
2	酚酸类Phenolic acids	88
3	脂质Lipid	65
4	氨基酸及其衍生物Amino acids and their derivatives	57
5	有机酸Organic acid	49
6	核苷酸及其衍生物Nucleotides and their derivatives	38
7	生物碱Alkaloid	20
8	木脂素和香豆素Lignin and coumarin	14
9	萜类Terpene	12
10	鞣质Tannin	3
11	其他类Others	53