葡萄,高温胁迫,恢复,光合作用,光系统Ⅱ," /> 葡萄,高温胁迫,恢复,光合作用,光系统Ⅱ,"/> Vitis vinifera L.,high temperature,recovery,photosynthesis,PSⅡ
,"/> <font face="Verdana">高温胁迫对‘赤霞珠’葡萄光合作用的影响</font>

中国农业科学 ›› 2010, Vol. 43 ›› Issue (13): 2744-2750 .doi: 10.3864/j.issn.0578-1752.2010.13.014

• 园艺 • 上一篇    下一篇

高温胁迫对‘赤霞珠’葡萄光合作用的影响

罗海波,马苓,段伟,李绍华,王利军   

  1. (中国科学院植物研究所)
  • 收稿日期:2009-12-15 修回日期:2010-03-05 出版日期:2010-07-01 发布日期:2010-07-01
  • 通讯作者: 王利军

Influence of Heat Stress on Photosynthesis in Vitis vinifera L. cv. Cabernet Sauvignon #br#

LUO Hai-bo,MA Ling, DUAN Wei, LI Shao-hua, WANG Li-jun   

  1. (中国科学院植物研究所)
  • Received:2009-12-15 Revised:2010-03-05 Online:2010-07-01 Published:2010-07-01
  • Contact: WANG Li-jun

摘要:

【目的】研究成熟期‘赤霞珠’葡萄的光合作用在高温胁迫下及恢复期间的变化,探讨葡萄光合作用抑制及恢复的机制。【方法】以‘赤霞珠’葡萄为试材,进行6 d 的高温(每日9:30—15:30 40℃,其余时间30℃)处理, 然后恢复 1 d。对照的温度每日6:30—18:30为30℃,其余时间20℃。恢复时的温度条件同对照的温度。测定高温处理前、处理过程中和恢复后葡萄叶片的气体交换参数和叶绿素荧光参数。【结果】每日40℃的高温处理后,葡萄叶片净光合速率(Pn)、气孔导度(Gs)、PSⅡ的实际光化学效率(ΦPSII)、光化学猝灭系数(qP)和单位面积有活性的反应中心的密度(RC/CSo)显著小于对照,细胞间隙二氧化碳浓度(Ci)、非光化学猝灭系数(NPQ)、光系统Ⅱ受体侧放氧复合体功能参数(Wk)和捕获的激子将电子传递到电子传递链中QA- 下游的其它电子受体的概率(ψEo)升高。经过1 d的恢复,高温处理的这些参数值与对照没有显著差异。【结论】40℃的高温胁迫导致‘赤霞珠’葡萄叶片的Pn显著下降,不过在常温下1 d就能够恢复。Pn下降不是气孔因子导致的结果,主要与PSⅡ供体侧和反应中心活性以及激发能的分配有关;Pn恢复上升,与上述因子都有关系。

关键词: 葡萄')">葡萄, 高温胁迫, 恢复, 光合作用, 光系统Ⅱ

Abstract:

【Objective】 Changes in photosynthesis in Vitis vinifera L. cv. Cabernet Sauvignon exposed to high temperature for extended periods and following recovery were investigated to reveal the mechanism of inhibition and recovery of photosynthesis. 【Method】 Two years old Cabernet Sauvignon grapevines were treated for 6 d at high temperature (40℃ from 9:30 to 15:30 and 30℃ during the rest of each day) and then at the same temperatures as the controls. The controls were treated at 30℃ from 6:30 to18:30 and 20℃ during the remainder of each day. Gas exchange and chlorophyll fluorescence parameters were measured before and during high temperature and following recovery. 【Result】 With heat stress, net photosynthetic rate (Pn), stomatal conductance (Gs), the actual PSⅡ efficiency (ΦPSII), the photochemical quenching coefficient (qP), and amount of active PSⅡ RCs per CS (RC/CSo) decreased, intercellular CO2 concentration (Ci), non-photochemical quenching (NPQ), and the damage of oxygen evolving complex indicated by WK, the probability that a trapped exciton moves an electron into the electron transport chain beyond QA- (ψEo) increased. When the grapevines recovered for 1 d from heat stress, the values of these parameters were similar to those in the controls. 【Conclusion】 Pn was decreased by heat stress at 40℃, but recovered to the control level after 1 d. The decrease in Pn were not due to stomatal limitations, but were associated with the donor side and reactive center of PSⅡ. The increase in Pn should be associated with the above all factors.

Key words: Vitis vinifera L.')">Vitis vinifera L., high temperature, recovery, photosynthesis, PSⅡ