中国农业科学 ›› 2020, Vol. 53 ›› Issue (21): 4365-4375.doi: 10.3864/j.issn.0578-1752.2020.21.006
高春华1,2(),冯波1(),曹芳1,李升东1,王宗帅1,张宾1,王峥1,孔令安1,王法宏1
收稿日期:
2020-05-11
接受日期:
2020-07-29
出版日期:
2020-11-01
发布日期:
2020-11-11
通讯作者:
冯波
作者简介:
高春华,E-mail:基金资助:
GAO ChunHua1,2(),FENG Bo1(),CAO Fang1,LI ShengDong1,WANG ZongShuai1,ZHANG Bin1,WANG Zheng1,KONG LingAn1,WANG FaHong1
Received:
2020-05-11
Accepted:
2020-07-29
Online:
2020-11-01
Published:
2020-11-11
Contact:
Bo FENG
摘要:
【目的】明确不同施氮量对高温胁迫后小麦同化物积累和转运的影响及其生理基础,以期为小麦抗逆稳产栽培提供技术和理论依据。【方法】于2018—2019年在济南和济阳两地进行,以济麦44为材料,田间搭建高温棚进行高温胁迫处理,设置2个温度处理(CK:未胁迫,H:花后高温胁迫),3个氮肥水平(低氮N1:180 kg·hm-2,常规氮N2:240 kg·hm-2,高氮N3:300 kg·hm-2)。通过分析小麦花前同化物质的转运、成熟期同化物质的积累与分配、叶片与籽粒中蔗糖合成酶在同化物转运中的作用,阐明了不同施氮量对花后高温胁迫后小麦籽粒产量形成的影响机制。【结果】不同施氮量对高温胁迫后小麦的减产率影响不同,N1处理减产率为54.78%(济南)和50.19%(济阳),N2处理为24.05%(济南)和25.29%(济阳),N3处理为54.49%(济南)和44.13%(济阳)。高温胁迫后,与N1和N3处理相比,N2处理成熟期同化物积累量、花前营养器官同化物向籽粒中转运量和转运率、花后同化物积累量和积累率、同化物向籽粒中的分配比例均显著增加;N2处理旗叶SPAD值、蔗糖合成酶SS-Ⅱ合成方向活性和籽粒蔗糖合成酶SS-Ⅰ分解方向活性增加。【结论】本试验条件下,施氮量为240 kg·hm-2可以显著减缓高温胁迫后旗叶衰老,维持旗叶中SS-Ⅱ和籽粒中SS-Ⅰ较高的酶活性,保持较高的同化物合成能力和向籽粒中的转运能力,提高同化物向籽粒中的积累量和分配比例,降低高温胁迫后小麦籽粒产量的损失率。
高春华,冯波,曹芳,李升东,王宗帅,张宾,王峥,孔令安,王法宏. 施氮量对花后高温胁迫后小麦同化物积累、转运及产量的影响[J]. 中国农业科学, 2020, 53(21): 4365-4375.
GAO ChunHua,FENG Bo,CAO Fang,LI ShengDong,WANG ZongShuai,ZHANG Bin,WANG Zheng,KONG LingAn,WANG FaHong. Effects of Nitrogen Application Rate on Assimilate Accumulation, Transportation and Grain Yield in Wheat Under High Temperature Stress After Anthesis[J]. Scientia Agricultura Sinica, 2020, 53(21): 4365-4375.
表1
高温胁迫和施氮量对小麦产量构成因素及同化物积累的影响"
处理 Treatment | 单穗重 WPP (g) | 穗粒数 GWPP | 千粒重 TKW (g) | 成熟期同化物积累量 AAAM (kg·hm-2) | 花后同化物积累速率AARAA (kg·hm-2·d-1) | 收获指数 HI (%) |
---|---|---|---|---|---|---|
济南JN | ||||||
CKN1 | 1.29±0.03b | 34.50±0.5c | 47.30±0.30a | 15785.52±114.40b | 157.16±1.03b | 50.92±0.63a |
CKN2 | 1.46±0.008a | 35.50±0.1b | 45.03±0.66b | 16967.30±283.20a | 182.83±1.35a | 47.96±1.65a |
CKN3 | 1.56±0.02a | 36.80±0.1a | 43.83±0.04c | 17716.02±607.11a | 180.31±12.75a | 48.24±1.53a |
HN1 | 0.87±0.06c | 29.75±0.25e | 19.56±0.24d | 13697.32±155.75c | 93.07±2.73c | 26.53±0.39c |
HN2 | 1.24±0.05b | 32.05±0.35d | 37.14±0.27c | 15671.66±173.46b | 138.45±1.64b | 39.41±0.09b |
HN3 | 0.94±0.02c | 30.45±0.05e | 17.12±0.54e | 14399.02±143.37c | 107.02±5.20c | 27.57±0.79c |
济阳JY | ||||||
CKN1 | 1.30±0.003c | 34.00±0.8b | 49.15±0.23a | 16190.53±79.64c | 153.17±0.002c | 45.00±0.50ab |
CKN2 | 1.40±0.003b | 36.60±0.2a | 45.32±0.03a | 16883.76±50.68b | 166.55±3.67b | 46.48±0.01a |
CKN3 | 1.66±0.004a | 37.75±0.05a | 44.05±0.36a | 18902.49±8.95a | 203.00±1.30a | 43.99±0.40b |
HN1 | 1.00±0.004e | 30.50±0.50c | 21.41±3.53b | 14331.65±52.49f | 103.47±1.36e | 25.33±0.69e |
HN2 | 1.13±0.14d | 32.95±0.15b | 38.71±1.25c | 15392.29±69.81d | 116.66±2.48d | 37.94±1.13c |
HN3 | 1.01±0.01e | 30.80±0.9c | 24.54±0.29c | 14565.30±94.87e | 88.96±0.56f | 31.89±0.41d |
FH | 691.42** | 369.32** | 885.17** | 368.11** | 691.45** | 1028.87** |
FN | 47.68** | 27.25** | 73.18** | 49.49** | 34.07** | 58.12** |
FH×N | 48.21** | 14.68** | 90.81** | 34.47** | 31.95** | 64.22** |
表2
花前同化物向籽粒的转运量及花后同化物积累量对高温胁迫和施氮量的响应"
处理 Treatment | 花前同化物AABA | 花后同化物AAAA | ||||
---|---|---|---|---|---|---|
转运量 TA (kg·hm-2) | 转运率 TR (%) | 籽粒贡献率 CR (%) | 积累量 AA (kg·hm-2) | 籽粒贡献率 CR (%) | ||
济南JN | ||||||
CKN1 | 2213.18±143.97b | 21.18±1.08a | 27.55±0.07b | 5343.56±35.02b | 72.45±0.07cd | |
CKN2 | 2144.03±116.87b | 19.93±0.65a | 26.39±0.10b | 6216.09±45.84a | 73.61±0.10c | |
CKN3 | 2655.26±128.60a | 22.91±0.77a | 31.10±1.43a | 6130.58±433.58a | 68.90±1.43d | |
HN1 | 746.73±106.73d | 7.07±0.85c | 20.49±0.40c | 3164.33±92.76c | 79.51±1.40b | |
HN2 | 1224.37±113.86c | 11.15±0.81b | 19.30±1.08c | 4707.26±55.68b | 80.20±1.58b | |
HN3 | 635.84±133.17d | 5.88±1.06c | 15.90±0.74d | 3638.70±176.82c | 84.10±0.74a | |
济阳JY | ||||||
CKN1 | 2020.81±16.37b | 18.93±0.14a | 27.74±0.40a | 5514.26±0.08c | 72.26±0.40b | |
CKN2 | 2145.54±48.82a | 19.26±0.64a | 27.34±0.71a | 5995.88±132.08b | 72.66±0.71b | |
CKN3 | 2255.84±37.18a | 19.43±0.39a | 27.14±0.68a | 7308.18±46.95a | 72.86±0.68b | |
HN1 | 653.49±28.92e | 6.16±0.27d | 18.00±0.37d | 3724.92±48.83e | 82.00±0.37a | |
HN2 | 1696.53±3.97c | 15.17±0.11b | 29.07±0.80a | 4199.76±89.11d | 70.93±0.80b | |
HN3 | 1320.79±35.39d | 11.63±0.35c | 28.44±0.94a | 3202.57±20.10f | 71.56±0.94b | |
FH | 516.60** | 765.81** | 193.99** | 726.97** | 128.53** | |
FN | 20.79** | 20.43** | 11.00** | 34.93** | 7.92** | |
FH×N | 23.5794** | 31.54** | 15.83** | 34.37** | 11.83** |
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