Dongxiang wild rice (Oryza rufipogon Griff., DWR) is an accession of common wild rice collected from
Dongxiang County, Jiangxi Province, China. It is one of the second class national protected wild plants in
China. Our previous results and other studies confirmed that DWR has owned many useful genes lacking
in the cultivated rice, including strong cold tolerance, drought resistance, P-deficiency tolerance, etc.
Among these useful agronomic characteristics, the cold tolerance is the most significant feature. DWR can
successfully overwintering in Wuhan City, Hubei Province, China, where the minimum temperature reaches
to –12°C in winter. In order to develop useful germplasms with unique gene from DWR and utilize these
germplasms for cultivated rice improvement, we developed a set of BC1F10 backcross inbred lines (BILs)
through a backcrossing and single-seed descent program. Firstly, an elite indica-cultivated rice Xieqingzao B
(O. sativa L. ssp. indica, recurrent parent) and DWR (donor parent) were used to produce F1 hybrid (left
picture). Afterwards, the hybrid F1 was backcrossed to Xieqingzao B to create BC1F1 population. Finally,
the BC1F1 population selfed consecutively to obtain BC1F10 inbred lines using single-seed descent method.
The BC1F10 population was used for interesting traits assessment and QTL analysis. Some elite introgression
lines (ILs, right picture) with interesting genes from DWR have been identified. And these elite ILs would be
helpful for cloning and utilizing useful genes from wild rice. See pages 265–275 by LUO et al. in details.