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| Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis |
| WANG Xiao-hui1*, WANG Yan-fang1, 2*, HUANG Hai-bi1, BAI Fan1, SHI Xiao-na1, MA Chang-jiao1, GAO Yuan1, ZHANG Jian-hua1, ZHANG Wen-guang3, HAO Yong-qing1 |
1 Key Laboratory of Microbiology & Immunology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China
2 Foundation Medicine College, Baotou Medical School, Baotou 014040, P.R.China
3 Key Laboratory of Animal Genetics and Genomics, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China |
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Abstract It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to mycoplasma research and application, including Mycoplasma mycoides subsp. capri (Mmc). Furthermore, little research on the characteristics of Mmc metabolism has been reported. In this study, Mmc PG3 strain cultures were investigated for dynamic gene expression. Culture samples were harvested during logarithmic phase (PG3-1), stationary phase (PG3-2), decline phase (PG3-3) and late decline phase (PG3-4). Twelve RNA samples (three replicates for each of the four growth stages considered) from these cultures were collected and sequenced. Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes (P<0.01) were linked to PG3 metabolism. The enzymes these genes coded were mainly involved in ATP synthase, pyrimidine metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism. Among these, cytidylate kinase, fructose 1,6-bisphosphate aldolases Class II, nicotinate-nucleotide adenylyltransferase and dihydrolipoamide dehydrogenase play a key role in Mmc metabolism. These results provide a baseline to build our understanding of the metabolic pathway of Mmc.
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Received: 04 July 2017
Accepted:
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| Fund: This work was supported by the Science and Technology Project of Inner Mongolia, China (201502070). |
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Corresponding Authors:
Correspondence HAO Yong-qing, E-mail: haoyq1960@163.com; ZHANG Wen-guang, E-mail: atcgnmbi@aliyun.com
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| About author: WANG Xiao-hui, Mobile: +86-15034782080, E-mail: wangxiaohui_hao@126.com; WANG Yan-fang, Mobile: +86-15849279411, E-mail: wyf-nmgnd@163.com; * These authors contributed equally to this study. |
Cite this article:
WANG Xiao-hui, WANG Yan-fang, HUANG Hai-bi, BAI Fan, SHI Xiao-na, MA Chang-jiao, GAO Yuan, ZHANG Jian-hua, ZHANG Wen-guang, HAO Yong-qing .
2018.
Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis. Journal of Integrative Agriculture, 17(2): 428-435.
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