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Journal of Integrative Agriculture  2018, Vol. 17 Issue (2): 348-358    DOI: 10.1016/S2095-3119(17)61653-8
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Identification and expression of the CEP gene family in apple (Malus×domestica)
LI Rui1, AN Jian-ping1, YOU Chun-xiang1, SHU Jing2, WANG Xiao-fei1, HAO Yu-jin
1 National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology/College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
2 Shandong Agriculture and Engineering University, Jinan 251100, P.R.China
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Abstract  Plant peptide hormones play important roles in plant growth and development.  Among these hormones, the C-TERMINALLYENCODED PEPTIDE (CEP) belongs to a newly found peptide family that regulates root development in Arabidopsis as well as in other species.  However, nothing is known about the CEP genes in apple (Malus×domestica, MdCEP).  In this study, a total of 27 apple CEP genes were identified through a genome-wide analysis and were phylogenetically divided into three classes (I, II and III).  The predicted MdCEP genes were distributed across 10 of 17 chromosomes with different densities.  Next, the gene structures and motif compositions of the MdCEP genes were analyzed.  Subsequently, the expression analysis suggested that the MdCEP genes were highly activated in roots and that MdCEP23 may play an important role in regulating the growth and development of roots.  Moreover, all of the MdCEP genes were responsive to multiple abiotic stresses, indicating that MdCEP genes may be involved with various aspects of physiological processes in apple.  Nearly one-third of MdCEP genes had a significant response to low nitrogen treatment.  Most of the MdCEP genes were up-regulated under stress, including mannitol, polyethylene glycol (PEG) and abscisic acid (ABA), suggesting that MdCEP genes may be involved in the drought stress response.  This study provides insight into the putative functions of the MdCEP genes using a genome-wide analysis of the CEP gene family. 
Keywords:   peptide signals        CEP gene family        expression analysis        apple  
Received: 02 February 2017   Accepted:

We thank the earmarked fund for the China Agriculture Research System (CARS-28), the National Natural Science Foundation of China (31471854, 31601742), the earmarked fund for the Shandong Province Agriculture Research, China (SDAIT-06-03), the Natural Science Foundation of Shandong Province, China (ZR2011CQ007).

Corresponding Authors:  Correspondence HAO Yu-jin, E-mail:; WANG Xiao-fei, E-mail:    
About author:  LI Rui,E-mail:

Cite this article: 

LI Rui, AN Jian-ping, YOU Chun-xiang, SHU Jing, WANG Xiao-fei, HAO Yu-jin . 2018. Identification and expression of the CEP gene family in apple (Malus×domestica). Journal of Integrative Agriculture, 17(2): 348-358.

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