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| Morphological, Biochemical and Genetic Analysis of a Brittle Stalk Mutant of Maize Inserted by Mutator |
| FU Xue-qian, FENG Jing, YU Bin, GAO You-jun, ZHENG Yong-lian, YUE Bing |
1.National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R.China
2.Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, P.R.China |
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摘要 Mutants on stalk strength are essential materials for the studies on the formation of plant cell wall. In this study, a brittle stalk mutant of maize, designated as Bk-x, was screened from a Mutator inserted mutant library. At the germination and early seedling stage, the mutant plants were indistinguishable from the normal ones. However, all of the plant organs were brittle after the 5th-leaf stage and remained brittle throughout the rest of the growing period. Microstructure observation showed that the cell wall in vascular bundle sheath of Bk-x was thinner than that in normal plants. The leaf mechanical strength in Bk-x was 77.9% of that in normal plants growing at Xishuangbanna (BN), Yunnan province and that was 61.7% in Wuhan (WH), Hubei Province, China. The proportion of cellulose was 12.3% in Bk-x, which was significantly lower than that in normal plants (26.7%), while the soluble sugar content was 36.1% in Bk-x, which is significantly higher than that in normal plants (12.4%). Genetic analysis using two F2 populations and one F2:3 families demonstrated that the trait of brittle stalk is controlled by a single recessive gene.
Abstract Mutants on stalk strength are essential materials for the studies on the formation of plant cell wall. In this study, a brittle stalk mutant of maize, designated as Bk-x, was screened from a Mutator inserted mutant library. At the germination and early seedling stage, the mutant plants were indistinguishable from the normal ones. However, all of the plant organs were brittle after the 5th-leaf stage and remained brittle throughout the rest of the growing period. Microstructure observation showed that the cell wall in vascular bundle sheath of Bk-x was thinner than that in normal plants. The leaf mechanical strength in Bk-x was 77.9% of that in normal plants growing at Xishuangbanna (BN), Yunnan province and that was 61.7% in Wuhan (WH), Hubei Province, China. The proportion of cellulose was 12.3% in Bk-x, which was significantly lower than that in normal plants (26.7%), while the soluble sugar content was 36.1% in Bk-x, which is significantly higher than that in normal plants (12.4%). Genetic analysis using two F2 populations and one F2:3 families demonstrated that the trait of brittle stalk is controlled by a single recessive gene.
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Received: 21 November 2011
Accepted:
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| Fund: This work was supported by the National High-Tech R&D Program of China (2006AA10A106). |
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Corresponding Authors:
Correspondence YUE Bing, Tel: +86-27-87282689, E-mail: yuebing@webmail.hzau.edu.cn
E-mail: yuebing@webmail.hzau.edu.cn
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| About author: FU Xue-qian, Tel: +86-27-87282689, E-mail: fxq@webmail.hzau.edu.cn; |
Cite this article:
FU Xue-qian, FENG Jing, YU Bin, GAO You-jun, ZHENG Yong-lian, YUE Bing.
2013.
Morphological, Biochemical and Genetic Analysis of a Brittle Stalk Mutant of Maize Inserted by Mutator. Journal of Integrative Agriculture, 12(1): 12-18.
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