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Journal of Integrative Agriculture  2017, Vol. 16 Issue (06): 1268-1275    DOI: 10.1016/S2095-3119(15)61142-X
Section 4: Analysis of cellulosic ciomass Advanced Online Publication | Current Issue | Archive | Adv Search |
Effects of protein and lignin on cellulose and xylan anaylses of lignocellulosic biomass
James MacLellan1, Rui Chen1, Zhengbo Yue1, 2, Robert Kraemer1, Yan Liu1, Wei Liao1

1 Department of Biosystems and Agricultural Engineering, Michigan State University, MI 48824, USA

2 School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, P.R.China

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Abstract  Interactions of lignocellulosic components during fiber analysis were investigated using the highly adopted compositional analysis procedure from the National Renewable Energy Laboratory (NREL), USA.  Synthetic feedstock samples were used to study the effects of lignin/protein, cellulose/protein, and xylan/protein interaction on carbohydrate analysis.  Disregarding structural influence in the synthetic samples, lignin and protein components were the most significant (P<0.05) factors on cellulose analysis.  Measured xylan was consistent and unaffected by content variation throughout the synthetic analysis.  Validation of the observed relationships from synthetic feedstocks was fulfilled using real lignocellulosic feedstocks: corn stover, poplar, and alfalfa, in which similar results have been obtained, excluding cellulose analysis of poplar under higher protein content and xylan analysis of alfalfa under higher protein content.  The results elucidated that according to their protein and lignin contents of different lignocellulosic materials, accuracy of the NREL method on cellulose and xylan analyses could be improved by applying a stronger extraction step to replace water/ethanol extraction.
Keywords:   lignocellulosic biomass      cellulose      xylan      lignin      protein  
Received: 02 February 2015   Accepted:

This research has been supported by the Michigan Public Service Commission (PSC-09-19), USA, the Michigan Animal Agriculture Initiative, the Michigan State Agricultural Station, Michigan State University (MSU) AgBioResearch, and the MSU Vice President for Research and Graduate Studies, USA.

Corresponding Authors:  Wei Liao, Tel: +1-517-4327205, E-mail:    

Cite this article: 

James MacLellan, Rui Chen, Zhengbo Yue, Robert Kraemer, Yan Liu, Wei Liao. 2017. Effects of protein and lignin on cellulose and xylan anaylses of lignocellulosic biomass. Journal of Integrative Agriculture, 16(06): 1268-1275.

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